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1.
Andrologia ; 50(2)2018 Mar.
Article in English | MEDLINE | ID: mdl-28730747

ABSTRACT

We investigated the prevalence of 5HT2C receptor gene polymorphisms in Egyptian patients with lifelong premature ejaculation. A total of 350 participants were enrolled in a prospective study. Two hundred and forty-five cases with lifelong premature ejaculation joined this study, in addition to 105 controls. We instructed the partners of the cases to measure the IELT of the first intercourse only using a stopwatch for 1 month. Genotyping was carried out at the end of the study. The results showed that the majority of the patients and controls were Cys/Cys. A highly significant statistical association was found between the studied gene polymorphisms and IELT among cases (p-values = .009). The study emphasised the potential role of 5HT2C receptor gene polymorphisms in patients with lifelong premature ejaculation.


Subject(s)
Polymorphism, Genetic , Premature Ejaculation/genetics , Receptor, Serotonin, 5-HT2C/genetics , Adult , Case-Control Studies , Egypt , Genotyping Techniques , Humans , Male , Prospective Studies , Time Factors , Young Adult
2.
J Obstet Gynaecol Res ; 24(1): 21-5, 1998 Feb.
Article in English | MEDLINE | ID: mdl-9564101

ABSTRACT

OBJECTIVE: To evaluate the ability of leukocyte esterase dipstick test (LET) performed in cervical mucus to predict direct immunofluorescent (DIF)-verified Chlamydia trachomatis (Ct) cervical infection. METHOD: Eighty women with inflammatory cervico-vaginal smear were submitted to both an endocervical smear, to verify Ct cervicitis by DIF technique; and a cervical mucus sample to perform LET. Results of LET and DIF were matched. RESULTS: Among 30 Chlamydial positive subjects, cervical mucus esterase test was positive in 28 (93.3%); while among 50 Chlamydial negative controls, esterase test was negative in 43 subject (86%). The sensitivity, specificity, positive and negative predictive values for LET were 93.3%, 86%, 80% and 95% respectively. CONCLUSION: Cervical mucus LET is a simple, cost effective screening test, with promising accuracy, for Ct cervical infection among women with inflammatory cervico-vaginal smears.


Subject(s)
Carboxylic Ester Hydrolases/analysis , Cervix Mucus/enzymology , Chlamydia Infections/diagnosis , Chlamydia trachomatis , Mass Screening/methods , Uterine Cervicitis/diagnosis , Biomarkers/analysis , Chlamydia Infections/enzymology , Chlamydia Infections/pathology , Cohort Studies , Female , Fluorescent Antibody Technique, Direct , Humans , Pilot Projects , Predictive Value of Tests , Uterine Cervicitis/enzymology , Uterine Cervicitis/pathology , Vaginal Smears
3.
J Toxicol Clin Toxicol ; 31(1): 31-41, 1993.
Article in English | MEDLINE | ID: mdl-8433414

ABSTRACT

Venom from the ringhals cobra (H. haemachatus) caused extensive chemosis, prolonged corneal edema and marked miosis when applied locally to the eyes of two strains of rabbits: Buskat (albino) and Chinchilla (pigmented). In the pigmented rabbits, the corneal edema progressed to complete corneal opacification with corneal and conjunctival neovascularization that remained unchanged until the end of the experiment (70 d). In the albino rabbits the corneal cloudiness and conjunctivitis cleared within three weeks of venom instillation. Treatment with heparin and tetracycline markedly improved the corneal opacification syndrome, while treatment with specific antivenom resulted only in partial improvement. It is postulated that the venom owes its ocular effects to its cardiotoxin, the only venom fraction capable of inducing the corneal opacification syndrome. The protective effect of heparin is probably through its electrostatic binding to the cardiotoxin in the venom. Tetracycline could form ionic bonding at several sites in the strongly basic cardiotoxin. The corneal opacification syndrome was associated with the ability of the snakes to spit since venoms from the other non-spitting cobras were devoid of this activity.


Subject(s)
Corneal Opacity/chemically induced , Elapid Venoms/toxicity , Animals , Antivenins/therapeutic use , Corneal Neovascularization/chemically induced , Corneal Neovascularization/physiopathology , Corneal Opacity/drug therapy , Corneal Opacity/physiopathology , Elapid Venoms/antagonists & inhibitors , Female , Heparin/therapeutic use , Male , Rabbits , Snakes , Species Specificity , Tetracycline/therapeutic use
4.
J Toxicol Clin Toxicol ; 31(1): 45-62, 1993.
Article in English | MEDLINE | ID: mdl-8433415

ABSTRACT

Fractionation of H. haemachatus, N. nigricollis, N. nivea and N. melanoleuca venoms using Amberlite CG-50 and (NH4)HCO3 elution gradient chromatography yielded 11-13 fractions for each venom. One fraction, F X, from H. haemachatus, two fractions, F X and F XI, from N. nigricollis and one fraction, F VIII, from N. melanoleuca venoms possessed the whole of ocular activity of the venoms. The fractions were the only venom fractions that caused cardiac depressant activity; their effect was reversed by raising Ca++ concentration in the physiological solution; they did not influence the twitches of the phrenic nerve hemidiaphragm and guinea-pig ileum preparations. Further purification of the fractions on Sephadex G-50 followed by fractionation on Amberlite CG-50 yielded fractions free from phospholipase A2 activity but possessing the same ocular effects. Similarly, the cardiotoxin from commercial N. nigricollis venom caused the same ocular effects as the crude venom and its purified cardiotoxic fractions. All cardiotoxic fractions as well as N. nigricollis cardiotoxin, caused extensive chemosis, blepharitis and corneal opacification with corneal and subconjunctival neovascularization. On a weight basis, the cardiotoxins were weaker in their oculotoxic activity than the corresponding parent crude venoms possibly because of the potentiating effect of phospholipase A2 in the crude venoms. It is postulated that in spitting cobras the cardiotoxins are responsible for the corneal opacification syndrome. In other cobra venoms the stable binding of cardiotoxins with acidic proteins limits their possible ocular effects. Only in the venoms of the spitting species are the cardiotoxins present in an appropriately free form to cause the ocular opacification syndrome.


Subject(s)
Cobra Cardiotoxin Proteins/toxicity , Corneal Opacity/chemically induced , Animals , Chromatography, Ion Exchange , Cobra Cardiotoxin Proteins/isolation & purification , Corneal Opacity/physiopathology , Female , Guinea Pigs , Heart/drug effects , Male , Muscle, Smooth/drug effects , Rabbits , Rats
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