[Improvement of the health care delivery system in war-time: monitoring of servicemen's health status].

Authors came to conclusion that the constant monitoring of servicemen's health status allows the commanding officer to receive detailed information about combat effectiveness. In case of battle injury or trauma the information used by the medical service will be the base for early health care delivery and organization of evacuation. The information about health status of injured may be used as a base for diagnosis at all stages of evacuation. Authors came to conclusion that individual monitoring of health status will help to further health care delivery system and first stages of medical evacuation.

Nondenaturing electrophoresis. Fractionating of photosynthetic pigment--protein complexes and blood plasma proteins.

Efficient polyacrylamide gel electrophoresis of labile proteins and protein complexes is reviewed. If only 0.001-0.01% SDS is dissolved in the electrode buffers, the detergent does not exhibit denaturing activity and guarantees high quality of electrophoresis. Even the structure and oxygen-producing activity of the labile photosystem PS2 are preserved after electrophoretic separation of photosynthetic pigment--protein complexes from Anacystis nidulans R2 or other cyanobacteria. The overall spectra of absorption or fluorescence of isolated pigment--protein complexes are equal to the corresponding spectra of the photosynthetic membrane. The distribution of chlorophyll molecules between the components of the photosynthetic apparatus coincides in spectral analysis data and gel fraction densitometry. More than 15 electrophoretic fractions of pigment--protein complexes of chloroplasts from green algae and higher plants were observed including some fractions of PS1, some spectrally different forms of light harvesting pigment--protein complexes, and their oligomers. High resolving capacity of electrophoresis was demonstrated by separation of plasma proteins. Low denaturing activity and low thermal dissipation of the electrode buffer solution allow the use of large diameter tubes (3.5 and 8 cm) in polyacrylamide gel electrophoresis. The cell destruction time and the membrane dissolving time are minimized. The method of electrophoretic staining of the gels was tested.

[4-(n-Dimethylaminostyryl)-1-methylpyridinium fluorescence in a living cell]. / Fluorestsentsiia 4-(n-dimetilaminostiril)-1-metilpiridiniia v zhivoi kletke.

A fluorescent compound 4-(p-dimethylinostyryl)-1-methylpyridinium (DSM) has been synthesized, having the absorption maximum about 450 nm and the fluorescence maximum about 590 nm in a water solution. A considerable increase of its fluorescence intensity is found in DNA solutions. The binding of DSM with membranes leads to a shift of its fluorescence maximum to 550 nm. Polychromatic properties of DSM (green fluorescence in membranes, rich yellow - in energized mitochondria, red-orange - in nuclei) are found in DSM stained cells. DSM fluorescence is sensitive to changes in the energized state of cells; the uncupler dinitrophenol or respiration inhibitors-cyanide and amital-cause a strong decrease in the DSM fluorescence intensity in mitochondria. It is ascertained that DSM itself has a low toxicity with respect to cell energy: it had no influence on the mobility of Tetrahymena pyriformis during 23 hours after staining. Thus, DSM may be used as a fluorescent probe for live cells.