ABSTRACT
Peripheral blood leukocytic migratory activity (LMA) was studied in patients with primary or recurrent erysipelas. A screening cell migration test (SCMT) was used in vitro and it established the prognostic value of MAL parameters at week 1 after the onset of erysipelas. It has been shown that a rapid transition of LMA from the phase of acceleration to that of inhibition characterizes the formation of an adequate immune response, corresponds to the good course of the disease, and has a low likelihood of recurrences. The probability of a recurrence is much higher than that when LMA tends to transit from suppression to acceleration and when LMA parameters are constant in the phase of suppression or acceleration. No transition of LMA to the phase of suppression in early convalescence suggests that the formation of an immune response to streptococcus is delayed.
Subject(s)
Cell Movement/immunology , Erysipelas/immunology , Leukocytes/immunology , Streptococcus pyogenes/immunology , Erysipelas/diagnosis , Erysipelas/therapy , Female , Humans , Male , Prognosis , Recurrence , Time FactorsABSTRACT
Peripheral blood leukocytic migratory activity (LMA) was studied in 51 patients with recurrent erysipelas versus 63 patients with primary erysipelas. To reveal LMA, the authors employed in vitro a screening cell migration test as an indicator of the cooperation of T and B lymphocytes and macrophages, by stimulating with polysaccharide A, surface proteins, L-antigen, hyaluronidase, streptolysine-O, and a complete set of Grasse S. pyogenes. In patients with recurrent erysipelas, undulating LMA changes were found in the course of the disease in response to the stimulation with partial specific S. pyogenes antigens. There were differences in the time course of LMA changes, when stimulated with specific surface streptococcus antigens and with components of streptococcus with toxic activity. Significant LMA differences were found in relation to the pattern of a local process: active LMA changes from acceleration to suppression in the erythematous-hemorrhagic form and hyperergic reactions of LMA acceleration in the bullous-hemorrhagic one.
Subject(s)
Cell Movement/immunology , Erysipelas/immunology , Leukocytes/immunology , Streptococcus pyogenes/immunology , Antigens, Bacterial , Cell Migration Assays/methods , Female , Humans , Male , RecurrenceABSTRACT
Peripheral blood leukocytic migratory activity (LMA) was studied in 63 patients with primary erysipelas. To reveal LMA, a screening cell migration test (SCMT) was used as an indicator of the cooperation of T- and B-lymphocytes and macrophages in the stimulation with polysaccharide A, surface proteins, L-antigen, hyaluronidase, streptolysin O, a complete S. pyogenes antigen complex after Grasse. The prognostic value of MAL parameters was established at week 1 after the onset of erysipelas. A rapid transition of LMA from the phase of acceleration to that of inhibition was shown to characterize the formation of an adequate response, to correspond to the good course of the disease, and to be followed by the low likelihood of recurrences. The probability of a subsequent recurrence is much higher than that when LMA tends to transit from suppression to acceleration and when LMA parameters are constant in the phase of suppression or acceleration. No transition of LMA to the phase of suppression in early convalescence suggests that the formation of an immune response to streptococcus is delayed.
Subject(s)
Antigens, Bacterial/immunology , Cell Movement/immunology , Erysipelas/immunology , Streptococcus pyogenes/immunology , T-Lymphocytes/immunology , Antigens, Bacterial/pharmacology , Erysipelas/diagnosis , Female , Humans , Lymphocyte Activation , Male , T-Lymphocytes/drug effectsABSTRACT
Described are the results of approbation of the cell-migration screening test reflecting the outcome of cooperation between T- and B-cells and macrophages. It was shown, in adults and children with intestine infection, to be a highly-effective tool for the detection of suppressed immune response during early disease stages at stimulation in vitro by Shiga toxin at nano- and picogram concentration; it can also be used for the evaluation of the shaping specific anti-Shiga-toxic immune response. The parameters of the migration activity of peripheral-blood leucocytes at exacerbation and convalescence were demonstrated to correlate with the age of sick children and with the severity of intestine infection as well as with the level of a Shiga-like toxin detected in coprofilters and circulating immune complexes.
Subject(s)
Bacterial Infections/immunology , Cell Movement/drug effects , Intestinal Diseases/immunology , Leukocytes/immunology , Shiga Toxin/pharmacology , Adult , Age Factors , Bacterial Infections/microbiology , Cells, Cultured , Child , Child, Preschool , Hematologic Tests/methods , Humans , Intestinal Diseases/microbiology , Predictive Value of Tests , Shiga Toxin/immunologyABSTRACT
The paper is devoted to an analysis of the clinical manifestations of keratoconjunctivitis sicca in Sjogren's syndrome in combination with SLE, sclerodermia systematica, rheumatoid arthritis, and in Sjogren's disease. Some characteristic signs of a course of keratoconjunctivitis sicca in Sjogren's disease and syndrome were defined.
