ABSTRACT
In order to study the effects of salicylic acid on the growth and physiological performance of Ulva prolifera with different proliferative styles, we took U. prolifera from vegetative (VU) and spore proliferative cells (SU) as materials, and cultured them under different salicylic acid concentrations to investigate their growth rate, chlorophyll fluorescence, SOD and soluble protein content. The results showed that the growth of both VU and SU was promoted by low concentration of salicy-lic acid, especially for VU. Under 0.2 Μg·mL-1 salicylic acid treatment, VU showed the highest relative growth rate of 21.0%, and the maximum photochemical efficiency (Fv/Fm) increased by 9.8% compared with SU. Additionally, salicylic acid affected the SOD activity significantly, and the enzyme activity of VU increased by 52.0% and 198.6% under 0.2 and 0.5 Μg·mL- 1 salicylic acid treatment, and that of SU increased by 54.1% and 38.0%, respectively. Salicylic acid also promoted the relative electron transfer rate (rETR), photosynthesis and protein content of both VU and SU. In conclusion, salicylic acid benefited the growth of two kinds of U. prolifera, especially for VU.
Subject(s)
Salicylic Acid , Ulva , Chlorophyll , PhotosynthesisABSTRACT
To study the combined effects of exogenous salicylic acid (SA) and ultraviolet radiation (UV) on marine green algae Ulva prolifera under high (160 Μmol·m-2·s-1) and low (70 Μmol·m-2·s-1) light intensities, the growth, chlorophyll fluorescence parameters, photosynthetic rate of oxygen, superoxide dismutase (SOD) activity, soluble polysaccharide and soluble protein contents were investigated after they grew with or without ultraviolet (UV, 3.2 W·m-2) radiation in the presence or absence of SA (10 Μg·mL-1) for three days. The treatments included control group (CK), SA, UV and UV+SA treatments. Results showed that under the low light intensity without UV condition, the relative growth rate was enhanced, Chl a and soluble protein contents were decreased by SA. Under the high light intensity without UV condition, the relative growth rate was decreased, Chl a content, respiratory rate, photosynthetic rate of oxygen, soluble polysaccharide and soluble protein contents were enhanced by SA. Under the high light intensity with UV condition, the relative growth rate, Chl a and soluble polysaccharide contents were enhanced by UV+SA. Additionally, under the low light intensity with UV condition, compared to UV treatment, the maximum photochemical efficiency (Fv/Fm) and soluble protein contents were respectively increased by 139.8% and 32.2% under the UV+SA treatment. In conclusion, SA reduced the inhibitory effects of U. prolifera induced by UV, and the effects were more significant under the high light intensity.
Subject(s)
Salicylic Acid , Ultraviolet Rays , Ulva , Chlorophyll , Light , PhotosynthesisABSTRACT
Hemophilia A (Hemophilia A, HA) is an X-linked recessive hereditary coagulation function disorder, the deficiency and dysfunction of blood coagulation were caused by the mutations of gene encoding clotting factor VIII. The treatment of hemophilia A still depends on the replacement therapy with blood coagulation factor. However, the repeated infusion of clotting factor will produce the neutralizing antibody against FVIII, then resulting in one of the serious complications. The reports on the incidence of inhibitor are different at home and abroad. Due to diverse factors, the inhibitors of hemophilia A clotting factor mainly can be divided into genetic and environmental factors, In this review, the inhibitors of hemophilia A clotting factor and their risk factors are briefly summarized.
ABSTRACT
In order to study the combined effects of brassinosteroids and salinity on the growth and physiological performance of Ulva prolifera under low temperature condition, we investigated the growth, chlorophyll content, chlorophyll fluorescence parameters, soluble protein and carbohydrate contents in this algae, which was grown under three salinity in the presence or absence of 24-epibrassinolide. The results showed that, compared to control salinity (25) treatment, the growth rate of U. prolifera was enhanced by 45.9% under the moderate hyposaline condition (10), but decreased under low salinity (5) treatment, which showed high contents of chlorophyll and soluble protein. However, the presence of EBR (0.2 mg·L-1) significantly reduced the growth of U. prolifera, especially under the control salinity (25) treatment, under which the fresh mass decreased and more spores were released. Additionally, the effective quantum yield (Fv'/Fm'), the activity of SOD and the content of soluble carbohydrate also decreased, but the soluble protein content increased under the control salinity treatment in the presence of EBR. In conclusion, moderatehypo-saline condition could be used to enhance the growth of U. prolifera at 15 â, and under normal salinity (25), the EBR could be used to promote the release of spores and produce more materials for mass production of U. prolifera.
Subject(s)
Brassinosteroids/pharmacology , Salinity , Steroids, Heterocyclic/pharmacology , Ulva/growth & development , Chlorophyll/analysis , Cold Temperature , Sodium ChlorideABSTRACT
The aim of study was to investigate the relationship between polymorphisms of FCGR2B232 1/T oligonucleotide and the susceptibility of children with idiopathic thrombocytopenic purpura (ITP). DNA from 76 patients with ITP and 37 controls was extracted. The SNPs of FCGR2B-232 was detected by polymerase chain reaction (PCR) combined with direct sequencing. The genotype distribution and allele frequency among different groups were compared. The results showed that the genotype (I/I, I/T, T/T) of FCGR2B-232 were 55.3%, 42.1%, and 2.6% in 76 patients with ITP, while 81.1%, 18.9%, 0% in 37 controls. The allele frequencies of FCGR2B-232 in patients with ITP were 76.3% (I232) and 23.7% (T232), but 90.5% and 9.5% in controls. There were significant differences in genotype distributions between the ITP patients and controls (chi(2) = 7.45, = 0.024). The enrichment in Thr232 allele carrier was also significant among the ITP patients as compared with the controls (chi(2) = 7.18, p = 0.007, odds ratio 3.47). There were also significant differences in allele frequencies between the ITP patients and controls [chi(2) = 6.54, p = 0.011, odds ratio 2.97, 95% CI (1.25 - 7.05)]. It is concluded that the polymorphisms of FCGR2B-232 significantly correlates with the susceptibility of children suffering from ITP. The minor Thr232 allele may be a risk genetic factor to ITP children.
Subject(s)
Child , Child, Preschool , Humans , Infant , Alleles , Case-Control Studies , Gene Frequency , Genetic Predisposition to Disease , Genotype , Polymorphism, Genetic , Purpura, Thrombocytopenic, Idiopathic , Genetics , Receptors, IgG , GeneticsABSTRACT
Objective To evaluate the relationship between Henoch-Schonlein purpura (HSP) accompanying renal impairment and helicobater pylori(Hp) infection.Methods This study consisted of 304 patients with HSP.The patients were divided into 2 groups(group A and group B) based on Hp infection or not(91 cases in group A and 213 cases in group B).Compared with the rates of accompanying renal impairment in 2 groups.And observed the recovery from renal impairment between the patients who were turned into negative(group C)and patients still were positive after the anti-Hp therapy(group D).Numeration data were analyzed by ?2 test.Results Group A which was with Hp infected,the accompanying renal impairment ratio was 65.9%.Group B which was without Hp infected,the ratio was 35.2%.There was significant difference between 2 groups(?2=24.378 P
