ABSTRACT
Alkaptonuria is a rare autosomal recessive disorder of metabolism caused by deficiency of homogentisic acid oxidase and resulting in accumulation of homogentisic acid in collagenous structures. This causes the classic clinical triad: (1) homogentisic aciduria (urine blackens on standing when oxidized or alkalinized); (2) eumelanin-like pigmentation of skin, sclera, cartilages, etc and (3) degenerative ochronic arthropathies usually in the fourth decade of life. Other important but more rare consequences of alkaptonuric ochronosis are cardiovascular and urinary tract involvement. We present a case of ochronosis with multiple visceral involvement: skin (fingers, ear sclera), severe spondylarthropaty with extensive calcifications of intervertebral discs and reduced mobility, osteoarthritis of both knees, right hip ostonecrosis, cardiovascular involvement (severe stenosis and insufficiency of aortic valve that) and urinary tract involvement (nephrolitiasis)
Subject(s)
Homogentisate 1,2-Dioxygenase/deficiency , Age Factors , Alkaptonuria , Aortic Valve Stenosis/etiology , Diagnosis, Differential , Ear, External/pathology , Fingers/pathology , Humans , Male , Middle Aged , Nephrolithiasis/etiology , Ochronosis/complications , Ochronosis/diagnosis , Radiography , Spondylarthropathies/diagnostic imaging , Spondylarthropathies/etiologyABSTRACT
Immunologic abnormalities observed in Systemic Sclerosis (SSc) patients consist of chronic mononuclear cell infiltration of affected tissues, dysregulation of lymphokine and growth factor production, and autoantibodies production. Expansion of CD4+T cells within the tissue seems to involve their activation that precedes this process. Therefore, CD4+T cells activation, as an early immune event, appears to be an important process in the development and maintaining of SSc. In SSc the disturbance of peripheral tolerance mechanisms could be also responsible for CD4+T cells activation. Consequently, we reevaluated CD4+T cells positive for CD25, GITR, CTLA-4, CD45RO, or Foxp3 in SSc patients, by comparison with healthy donors (HDs), and in correlation with clinical features of the disease. Our results reargued for activation of peripheral blood CD4+T cells in SSc patients. Thus, increased percentages of CD25+ and GITR+ CD4+T cells were found in SSc patients by comparison with HDs. Direct correlation between the percentage of GITR+CD4+T cells and disease activity recommended these cells as a good candidate for disease progression. In SSc patients, the negative regulators of T cells activation are also affected. Thus, CTLA-4+ and Foxp3+ CD4+T cell percentages were significantly reduced in SSc patients when compared to HDs. Indirect correlation between the percentage of CD152+CD4+T cells and autoantibodies (aScl70) presence or disease type highlighted the role of these cells in the disturbance of peripheral tolerance. The absence of the direct correlation between CD152+CD4+T cells and CD45RO+CD4+T cells, correlation observed only in HDs, raised the hypothesis that in SSc patients, memory T cells can be easily activated, and by consequence, they can enter within affected tissues. These data reconfirm the activation state of SSc CD4+T cells and point out some abnormalities in peripheral tolerance mechanisms that can contribute to SSc pathogeny.
Subject(s)
CD4-Positive T-Lymphocytes/immunology , Immunity, Cellular , Scleroderma, Systemic/immunology , Antigens, CD/biosynthesis , Antigens, CD/immunology , CD4-Positive T-Lymphocytes/metabolism , CD4-Positive T-Lymphocytes/pathology , CTLA-4 Antigen , Disease Progression , Flow Cytometry , Forkhead Transcription Factors/biosynthesis , Forkhead Transcription Factors/immunology , Glucocorticoid-Induced TNFR-Related Protein , Humans , Immune Tolerance , Immunologic Memory , Interleukin-2 Receptor alpha Subunit/biosynthesis , Interleukin-2 Receptor alpha Subunit/immunology , Leukocyte Common Antigens/biosynthesis , Leukocyte Common Antigens/immunology , Lymphocyte Activation , Receptors, Nerve Growth Factor/biosynthesis , Receptors, Nerve Growth Factor/immunology , Receptors, Tumor Necrosis Factor/biosynthesis , Receptors, Tumor Necrosis Factor/immunology , Scleroderma, Systemic/metabolism , Scleroderma, Systemic/pathology , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , T-Lymphocyte Subsets/pathologyABSTRACT
El diagnóstico de los tumores que debutan con metástasis óseas supone un problema frecuente. La estrategia diagnóstica debe dirigirse hacia las neoplasias más frecuentes y hacia aquellas en las que el tratamiento pueda obtener resultado. El estudio del tubo digestivo en ausencia de síntomas, normalmente queda excluido de esta búsqueda. Presentamos un paciente joven con metástasis óseas en múltiples localizaciones, sin otras manifestaciones clínicas acompañantes, en el que se detectó la presencia de un cáncer gástrico como origen de las mismas (AU)
