ABSTRACT
The microbiological diagnosis of infection for hematological malignancy patients receiving chemotherapy or allogeneic hematopoietic stem cell transplantation (allo-HSCT) patients relies primarily on standard microbial culture, especially blood culture, which has many shortcomings, such as having low positive rates, being time-consuming and having a limited pathogenic spectrum. In this prospective observational self-controlled test accuracy study, blood, cerebrospinal fluid (CSF), and bronchoalveolar lavage fluid (BALF) samples were collected from chemotherapy or allo-HSCT patients with clinical symptoms of infections who were hospitalized at Peking University First Hospital. Possible pathogens were detected by the method based on recombinant mannan-binding lectin (MBL) magnetic bead enrichment (M1 method) and simultaneously by a standard method. The analytical sensitivity of M1 method was close to that of standard culture method. Besides, the turn-around time of M1-method was significantly shorter than that of standard culture method. Moreover, the M1 method also added diagnostic value through the detection of some clinically relevant microbes missed by the standard method. M1 method could significantly increase the detection efficiency of pathogens (including bacteria and fungi) in immunocompromised patients. KEY POINTS: ⢠The detection results of M1-method had a high coincidence rate with that of standard method ⢠M1 method detected many pathogens which had not been found by standard clinic method.
Subject(s)
Mannose-Binding Lectin , Humans , Bronchoalveolar Lavage Fluid , Bacteria , Immunocompromised Host , Magnetic Phenomena , High-Throughput Nucleotide SequencingABSTRACT
Aim To investigate the potential protective effect of quercetin on chronic ethanol exposure induced autophagy dysfunction .Methods The male C57 BL/6 J mice were divided randomly into four groups: Nor-mal control group ( Con);Ethanol group ( Eth);Etha-nol plus Quercetin group ( Eth+Qu); Quercetin con-trol group ( Qu ) .The mice were pair-fed with either regular or ethanol-containing Lieber De Carli liquids diets for 15 weeks and the related parameters were as-sayed .Result Compared to Con group , chronic etha-nol feeding led to serious aminotransferases leakage , hepatic lipid accumulation , and liver oxidative dam-age.Additionally, long-term ethanol exposure caused lysosome damage , cathepsin B leakage to cytoplasm and decreased the expression of LAMP 2A which was the key protein for the autophagosome-lysosome fusion . As expect , quercetin supplementation evidently allevia-ted lysosome damage and decreased the abnormal LC 3-II and p62 accumulation , accelerated the autophago-some-lysosome fusion and increased the expression of LAMP2A.Conclusion Quercetin may alleviate the liver injury induced by chronic alcohol exposure by a-meliorating the lysosomal damage and up-regulating LAMP2 A expression .
