Evaluation of three alum-precipitated Aeromonas hydrophila vaccines administered to Labeo rohita, Cirrhinus mrigala and Ctenopharyngodon idella: immunokinetics, immersion challenge and histopathology / Avaliação de três vacinas de Aeromonas hydrophila precipitadas com alúmen administradas a Labeo rohita, Cirrhinus mrigala e Ctenopharyngodon idella: imunocinética, desafio de imersão e histopatologia

Aeromonas hydrophila is a cause of infectious disease outbreaks in carp species cultured in South Asian countries including Pakistan. This bacterium has gained resistance to a wide range of antibiotics and robust preventive measures are necessary to control its spread. No prior use of fish vaccines has been reported in Pakistan. The present study aims to develop and evaluate inactivated vaccines against local strain of A. hydrophila in Pakistan with alum-precipitate as adjuvant. The immunogenic potential of vaccine was evaluated in two Indian major carps (Rohu: Labeo rohita, Mori: Cirrhinus mrigala) and a Chinese carp (Grass carp: Ctenopharyngodon idella). Fish were vaccinated intraperitoneally followed by a challenge through immersion. Fish with an average age of 4-5 months were randomly distributed in three vaccinated groups with three vaccine concentrations of 108, 109 and 1010 colony forming unit (CFU)/ml and a control group. Fixed dose of 0.1ml was applied to each fish on 1st day and a booster dose at 15 days post-vaccination (DPV). Blood samples were collected on 14, 28, 35, 48 and 60 DPV to determine antibody titers in blood serum using compliment fixation test (CFT). Fish were challenged at 60 DPV with infectious A. hydrophila with 108 CFU/ml through immersion. Significantly higher levels of antibody titers were observed from 28 DPV in all vaccinated groups as compared to those in the control group. In challenge experiment the average RPS (relative percent survivability) was 71% for groups vaccinated with 109 and 1010 CFU/ml and 86% for 108 CFU/ml. Vaccine with 108 CFU/ml induced highest immune response followed by 109 and 1010 CFU/ml. The immune response of L. rohita and C. idella was better than that of C. mrigala. In general, normal histopathology was [...].

Aeromonas hydrophila é uma causa de surtos de doenças infecciosas em espécies de carpas cultivadas em países do sul da Ásia, incluindo o Paquistão. Essa bactéria ganhou resistência a uma ampla gama de antibióticos, e medidas preventivas robustas são necessárias para controlar sua disseminação. Nenhum uso anterior de vacinas para peixes foi relatado no Paquistão. O presente estudo tem como objetivo desenvolver e avaliar vacinas inativadas contra cepa local de A. hydrophila no Paquistão com precipitado de alúmen como adjuvante. O potencial imunogênico da vacina foi avaliado em duas carpas principais indianas (Rohu: Labeo rohita, Mori: Cirrhinus mrigala) e uma carpa chinesa (Grass Carp: Ctenopharyngodon idella). Os peixes foram vacinados por via intraperitoneal, seguido de um desafio por imersão. Peixes com idade média de 4-5 meses foram distribuídos aleatoriamente em três grupos vacinados com três concentrações de vacina de 108, 109 e 1010 unidades formadoras de colônias (UFC) / ml e um grupo de controle. Foi aplicada dose fixa de 0,1ml em cada peixe no 1º dia e dose de reforço 15 dias pós-vacinação (DPV). Amostras de sangue foram coletadas em 14, 28, 35, 48 e 60 DPV para determinar os títulos de anticorpos no soro sanguíneo usando o teste de fixação de elogio (CFT). Os peixes foram desafiados a 60 DPV com infecciosa A. hydrophila com 108 CFU / ml por imersão. Níveis significativamente mais elevados de títulos de anticorpos foram observados em 28 DPV em todos os grupos vacinados, em comparação com aqueles no grupo de controle. Na experiência de desafio, o RPS médio (sobrevivência percentual relativa) foi de 71% para os grupos vacinados com 109 e 1010 CFU / ml e 86% para 108 CFU / ml. A vacina com 108 UFC / ml induziu a maior resposta imune seguida por 109 e 1010 UFC / ml. A resposta imune de L. rohita e C. idella foi melhor do que a de C. mrigala. Em geral, histopatologia normal foi observada em diferentes [...].

Review of potential risk groups for coronavirus disease 2019 (COVID-19).

The current pandemic of coronavirus disease 19 (COVID-19) is a global issue caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Studies have revealed that this virus results in poorer consequences and a higher rate of mortality in older adults and those with comorbidities such as cardiovascular disease, hypertension, diabetes and prolonged respiratory illness. In this review, we discuss in detail the potential groups at risk of COVID-19 and outline future recommendations to mitigate community transmission of COVID-19. The rate of COVID-19 was high in healthcare workers, smokers, older adults, travellers and pregnant women. Furthermore, patients with severe medical complications such as heart disease, hypertension, respiratory illness, diabetes mellitus and cancer are at higher risk of disease severity and mortality. Therefore, special effort and devotion are needed to diminish the threat of SARS-CoV-2 infection. Proper vaccination, use of sanitizers for handwashing and complete lockdown are recommended to mitigate the chain of COVID-19 transmission.

Evaluation of climate-induced waterlogging hazards in the south-west coast of Bangladesh using Geoinformatics.

Climate-induced waterlogging has been significantly affecting the lives and livelihood of people in the south-west coastal region of Bangladesh for a couple of decades. The objective of this study is to investigate the waterlogging hazards of Tala, a south-western coastal Upazila of Bangladesh by analyzing satellite image. An empirical model based on velocity of flow, depth of flow, and inundation depth has been proposed to assess waterlogging hazard in pre monsoon, monsoon, and post monsoon period. Landsat TM images for the years 1989, 2000, and 2011 were analyzed by using Geoinformatics including GIS and remote sensing techniques to quantify the water hazard. Three dominant land use classes such as "water," "vegetation," and "bare lands and others" were selected to identify the land use land cover change. Both FGD (focus group discussions) and KII (key informant interviews) were also accomplished to assess the waterlogging hazard. It was revealed that 0.7% of the study area (246 ha) was under water in 1989, which increased alarmingly to 34% (11,525 ha) in 2011. There was an increase in 62.9% of water bodies during 1989 to 2000, which was further expanded to 77% during 2000 to 2011. Satellite image analysis between 2011 and 2015 also showed that nearly 89% of the waterlogged area including floodplain is inundated by tidal saline water that supports shrimp cultivation. On the contrary, 11% of the waterlogged area was occupied by trapped rain water. Confirmation of saline water and fresh water was done by measuring electrical conductivity and conducting "mouth taste" during field visit. The decreasing rate of "bare lands and others" category indicates that there is around 69.4% of reduction in this category to accommodate the increasing water covering areas. The hazard model shows that the middle part of the Tala along with the flood plain of the Kabodak River usually have to suffer waterlogged in both pre monsoon and post monsoon period. It was found that low flow in the upstream side, siltation in the Kabodak River, elevated riverbed, and recent increase in total annual rainfall have resulted in waterlogging problem. Combining inputs obtained from FGD, KII, and economic study of drainage, the TRM (tidal river management) followed by re-excavation of silted riverbed may provide a sustainable solution to solve the prolonged waterlogging situation.

Care of terminally-ill patients: an opinion survey among critical care healthcare providers in the Middle East.

BACKGROUND: Modern medicine has allowed physicians to support the dying terminally-ill patient with artificial means. However, a common dilemma faced by physicians in general, and intensivist in particular is when to limit or withdraw aggressive intervention. OBJECTIVE: To study the effect of training background and seniority on Do-not to resuscitate (DNR) decisions in the Middle East. METHODS: Anonymous questionnaire sent to members of the Pan Arab Society of Critical Care. RESULTS: The response rate was 46.2%. Most of the responders were Muslim (86%) and consultants (70.9%). Majority of the responders were trained in western countries. Religion played a major role in 59.3% for making the DNR decision. DNR was considered equivalent to comfort care by 39.5%. In a futile case scenario, Do Not Escalate Therapy was preferred (54.7%). The likelihood of a patient, once labeled DNR, being clinically neglected was a concern among 46.5%. Admission of DNR patients to the ICU was acceptable for 47.7%. Almost one-half of the responders (46.5%) wanted physicians to have the ultimate authority in the DNR decision. Training background was a significant factor affecting the interpretation of the term no code DNR (P< 0.008). CONCLUSION: Training background and level of seniority in critical care provider does not impact opinion on most of end of life issues related to care of terminally-ill patients. However, DNR is considered equivalent to comfort care among majority of Middle Eastern trained physicians.

Length-weight relationship and condition factor of Liza subviridis (Valenciennes, 1836) of Parangipettai waters, southeast coast of India.

Determination of Length-weight Relationship (LWR) of any commercially important fish is crucial to validate the wild stock level, to predict their wellbeing in the natural habitat and for various sustainable fishery management practices. Liza subviridis (Valenciennes) is noted to be highly abundant along the coast of Parangipettai, South east coast of India. Hence, the present study was aimed to establish Length-weight relationship and condition factor of Greenback mullet, Liza subviridis (Valenciennes) occurring in Vellar estuary, Parangipettai (lat. 11 degrees 30' N, long. 79 degrees 46' E) using least square method. To determine the actual relationship between length and weight of L. subviridis exponent coefficient or equilibrium constant (b) and relative condition factor (Kn) analysis were adopted. The females were found to be heavier than males at similar length. The equilibrium constant 'b' was found to be 2.7106 in males and 2.8927 in females. The corresponding parabolic representation for male was W = 0.0462L(2.7106) and for female W = 0.0382L(2.8927). The equilibrium constant did not obey the cube law as it deviated significantly from 3 in the case of males. The relative condition factor around 1 and little over it revealed the well-being of L. subviridis in Parangipettai waters.

A pharmacological and phytochemical evaluation of medicinal plants used by the Harbang clan of the Tripura tribal community of Mirsharai area, Chittagong district, Bangladesh.

BACKGROUND: Recent years have witnessed a continual decline of medicinal plant availability as well as a decline in the numbers of tribal traditional healers and their medicinal knowledge in Bangladesh. Yet these medicinal plants used for treatment of various ailments by tribal traditional healers can be of considerable interest to modern science in their potential for discovery of lead compounds, which can lead to better drugs. OBJECTIVE: The primary objective of the present study was to conduct an ethnomedicinal survey among the traditional healers of the Harbang clan (Tripura tribe) of Mirsharai to gain information on medicinal plants used to treat various ailments with the further objective of evaluating the efficacy of these medicinal plants when compared with known phytochemicals and modern-research-based pharmacologic activity studies on these plants. METHODS: Semistructured interviews and guided field-walk methods were used to gather information on medicinal plants used by the Tripura traditional healers. Along with plants, information was also collected on plant parts used, formulations, and dosages. Information on phytochemicals as well as pharmacologic activity studies on these plants (if any) was obtained from several data bases. SETTING: The survey was conducted among the traditional healers of the Harbang clan (Tripura tribe) residing in Mirsharai, Chittagong district, Bangladesh. RESULTS: The traditional healers of the Tripura tribal community of Mirsharai use 64 plant species distributed into 38 families for treatment of various ailments. CONCLUSIONS: Information on phytochemicals and pharmacologic activity studies conducted on a number of the plants (used by Tripura tribal healers) by modern scientific methods validated the traditional use of a number of plants and suggested that they can form a good source of newer drugs. The survey further highlighted the importance of gathering such ethnomedicinal information for effective conservation of tribal medicinal knowledge and medicinally important plant species.

Trimetallic supported catalyst for renewable source of energy and environmental control through CO2 conversion.

A supported catalyst and a catalytic process have been developed for the conversion of carbgas (CO2 + (100 ppm) H2O + 1% H2) as a renewable source of energy and as a measure for the control of carbon dioxide -- a greenhouse gas. The carbgas was passed over a trimetallic supported catalyst consisting of ruthenium (Ru), manganese (Mn) and cobalt (Co) dispersed on a high surface area titanium dioxide support at 673 K and at atmospheric pressure with a gas space velocity of 6000-7200/h. The catalytic reaction produces methanol and propyne in a fixed bed reactor system. The catalyst simultaneously splits water into hydrogen and oxygen, and carbon dioxide into carbon and oxygen under very mild reaction conditions and at atmospheric pressure. The oxygen generated during the reaction and the addition of hydrogen during the catalytic reaction not only generates a considerable amount of energy for the reaction to proceed but also sustains the oxidation states of Ru, Mn and Co. This process maintains the specific active oxidation states of the metals during the catalytic run -- a key step in the process.

Propiedades antidiarreicas de Diospyros peregrina en el modelo de diarrea inducida con aceite de ricino en ratones / Anti-diarrhoeal properties of Diospyros peregrina in the castor oil-induced diarrhoea model in mice

Diospyros peregrina (Gruke), de la familia de las ebenáceas, posee varias propiedades medicinales y se utiliza enla medicina tradicional de Bangladesh. En este estudio se investigaron los extractos de metanol de la corteza y lassemillas de esta planta en el modelo de diarrea inducida con aceite de ricino en ratones. También se estudiaronlas propiedades antibacterianas de los extractos frente a varias bacterias patógenas causantes de diarrea y disenteríamediante el método de difusión en disco. Con una dosis de 250 y 500 mg/kg de peso corporal, los extractosretrasaron la aparición de la diarrea. Los extractos también redujeron la frecuencia y la gravedad de la diarrea enlos animales de los grupos de prueba durante todo el período de estudio. A igual dosis, los extractos retrasaron eltránsito intestinal del preparado de carbón en los animales del grupo de prueba en comparación con los del grupode control, y los resultados fueron signifi cativos estadísticamente. En ambos casos, el extracto de semilla resultó máspotente que el extracto de corteza. El extracto de corteza inhibió el crecimiento de Staphylococcus aureus, Shigelladysentery, Escherichia coli y Pseudomonas aeruginosa, mientras que el extracto de semilla demostró poseer actividadinhibitoria frente a S. aureus, S. dysentery y E. coli

Diospyros peregrina Gruke. (Ebenaceae) possesses a number of medicinal properties, and is used in Bangladeshi traditional ;;medicine. In this study, the methanol extracts of the barks and seeds of this plant have been investigated in ;;the castor oil induced diarrhoea model in mice. The extracts were also studied for their anti-bacterial properties by ;;disc diffusion method against a number of pathogenic bacteria responsible for causing diarrhoea and dysentery. At ;;the doses of 250 and 500 mg/kg body weight, the extracts delayed the onset of diarrhoea. The extracts also reduced the ;;frequency and severity of diarrhoea in test animals throughout the study period. At the same doses, the extracts delayed ;;the intestinal transit of charcoal meal in test animals as compared to the control, and the results were statistically signifi ;;cant. In both cases the seed extract was found to be more potent than the bark extract. The bark extract inhibited ;;the growth of Staphylococcus aureus, Shigella dysentery, Escherichia coli and Pseudomonas aeruginosa, whereas the ;;seed extract demonstrated inhibitory activity against S. aureus, S. dysentery and E. coli

Intercomparison of INAA and ICP-MS results for thorium determination in Pakistani diet.

A pilot study on ingestion and organ content of trace elements of importance in radiological protection was to be carried out in Pakistan. Baseline analytical data on daily dietary intake of thorium was to be measured using Instrumental Neutron Activation Analysis (INAA) technique. To determine the accuracy and reliability of our technique, some samples were measured in Pakistan using INAA and in Japan using Inductively Coupled Plasma-Mass Spectrometry. For intercomparison of results overall mean Z-scores were calculated. The results showed validity of our technique. Mean value of 232Th concentration in Pakistani diet samples using INAA technique is 0.0062 +/- 0.0028 microg/g and with ICP-MS technique is 0.0069 +/- 0.0032 microg/g.

Determination of Optical Constants of Solgel-Derived Inhomogeneous TiO(2) Thin Films by Spectroscopic Ellipsometry and Transmission Spectroscopy.

Amorphous and nanocrystalline TiO(2) thin films coated on a vitreous silica substrate by a solgel dip coating method are investigated for optical properties by spectroscopic ellipsometry (SE) together with transmission spectroscopy. A method of analysis of SE data to determine the degree of inhomogeneity of TiO(2) films has also been presented. Instead of the refractive index, the volume fraction of void has been assumed to vary along the thickness of the films and an excellent agreement between the experimental and calculated data of SE below the fundamental band gap has been obtained. The transmission spectrum of these samples is inverted to obtain the extinction coefficient k spectrum in the wavelength range of 300-1600 nm by using the refractive indices and parameters of structure determined by SE. The nonzero extinction coefficient below the fundamental band-gap energy (3.2 eV) has been obtained for the nanocrystalline TiO(2) and shows the presence of optical scattering in the film.

Effects of socioeconomic factors, psychological stress, smoking, alcohol and caffeine on preterm delivery.

The relation between preterm birth and socioeconomic and psychological factors, smoking, alcohol and caffeine consumption are studied. Gestational age was determined from ultrasound and maternal dates. Adverse social circumstances are associated with preterm birth which inturn effects fetal growth.