ABSTRACT
The hormone salicylic acid (SA) plays a crucial role in plant immunity by activating responses that arrest pathogen ingress. SA accumulation also penalizes growth, a phenomenon visible in mutants that hyperaccumulate SA, resulting in strong growth inhibition. An important question, therefore, is why healthy plants produce basal levels of this hormone when defense responses are not activated. Here, we show that basal SA levels in unchallenged plants are needed for the expression of a number of immunity-related genes and receptors, such as RECEPTOR-LIKE PROTEIN 23 (RLP23). This was shown by depleting basal SA levels in transgenic Arabidopsis lines through the overexpression of the SA-inactivating hydroxylases DOWNY MILDEW-RESISTANT 6 (DMR6) or DMR6-LIKE OXYGENASE 1. RNAseq analysis revealed that the expression of a subset of immune receptor and signaling genes is strongly reduced in the absence of SA. The biological relevance of this was shown for RLP23: In SA-depleted and SA-insensitive plants, responses to the RLP23 ligand, the microbial pattern nlp24, were strongly reduced, whereas responses to flg22 remained unchanged. We hypothesize that low basal SA levels are needed for the expression of a subset of immune system components that enable early pathogen detection and activation of immunity.
Subject(s)
Arabidopsis Proteins , Arabidopsis , Gene Expression Regulation, Plant , Plant Diseases , Plant Immunity , Salicylic Acid , Salicylic Acid/metabolism , Arabidopsis/genetics , Arabidopsis/immunology , Arabidopsis/microbiology , Plant Immunity/genetics , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Plant Diseases/microbiology , Plant Diseases/immunology , Plant Diseases/genetics , Plants, Genetically ModifiedABSTRACT
Plants possess an effective immune system to combat most microbial attackers. The activation of immune responses to biotrophic pathogens requires the hormone salicylic acid (SA). Accumulation of SA triggers a plethora of immune responses (like massive transcriptional reprogramming, cell wall strengthening, and production of secondary metabolites and antimicrobial proteins). A tradeoff of strong immune responses is the active suppression of plant growth and development. The tradeoff also works the opposite way, where active growth and developmental processes suppress SA production and immune responses. Here, we review research on the role of SA in the growth-immunity tradeoff and examples of how the tradeoff can be bypassed. This knowledge will be instrumental in resistance breeding of crops with optimal growth and effective immunity.
