Volatiles of rhizobacteria Serratia and Stenotrophomonas alter growth and metabolite composition of Arabidopsis thaliana.

The emission of volatiles is a common, but mostly neglected, ability of bacteria that is important for inter- and intraspecific interactions. Currently, limited information is available on how the bacterial volatile (mVOC) signal is integrated into a plant's life at the physiological, transcriptional and metabolic level. Previous results provided evidence for volatile-dependent regulation of WRKY18, a pathogen-responsive transcription factor of Arabidopsis thaliana in co-culture with two rhizobacteria, Serratia plymuthica HRO-C48 and Stenotrophomonas maltophilia R3089. Dual cultures of these bacteria and A. thaliana; application of the common mVOC 2-phenyl-ethanol; extraction of metabolites of A. thaliana after exposure to bacterial volatiles; and analysis of the metabolomes (GC-TOF/MS) were carried out. The prominent microbial aromatic compound 2-phenyl-ethanol, emitted by both bacteria, negatively affects growth of A. thaliana wild type, whereas WRKY18 T-DNA insertion mutants were significantly more tolerant than wild-type seedlings. This paper also demonstrates for the first time the impact of the rhizobacterial volatiles on the metabolome of A. thaliana. Upon mVOC exposure the plants rearrange their metabolism by accumulation of e.g. amino acids and TCA intermediates that potentially allow plants to cope with and survive this stress. Our findings illustrate the high degree of complexity of metabolic rearrangements underlying the interactions of bacterial volatile elicitors and resulting plant responses. Furthermore, the impact of the volatile 2-phenyl-ethanol as a signal in the WRKY18-dependent pathway highlights this compound as an important molecular player.

Electrodiffusional uptake of organic cations by pea seed coats. Further evidence for poorly selective pores in the plasma membrane of seed coat parenchyma cells.

In developing seeds, the permeability of the plasma membrane of seed coat parenchyma cells is crucial for the supply of nutrients to the embryo. Here, we report characteristics of the transport of the organic cation choline and the basic amino acid L- histidine (His; cation at pH 5, electroneutral at pH 7) into isolated seed coats of pea (Pisum sativum). Supplied at sub-micromolar concentrations, choline(+) accumulated in the seed coat tissue 5.1 +/- 0.8-fold, His(+) 2.4 +/- 0.3-fold, and His(0) 1.3 +/- 0.2-fold. Taking into consideration that at pH 5 His influxes as a cation but effluxes as a neutral molecule, these accumulations are in reasonable agreement with (electro) diffusional uptake at the prevailing membrane potential of -55 +/- 3 mV. At a concentration of 100 mM, choline(+) and His(+), but not His(0), depolarized the membrane of the parenchyma cells and neither of the substrates was accumulated. At this concentration, the relative influx (the ratio of influx and external concentration, a measure for membrane permeability) of choline and His was approximately 10 micromol g(-1) fresh weight min(-1) M(-1), similar to that found for neutral amino acids, sucrose, glucose, and mannitol. At lower concentrations, the relative influx of choline(+) and His(+) increased because of increasingly more negative membrane potentials, giving rise to apparent saturation kinetics. It is suggested that transport of organic cations can proceed by a general, poorly selective pore in the plasma membrane of seed coat parenchyma cells. This pore is thought to be responsible for the unloading of a range of solutes that serve as nutrients for the embryo.