Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Type of study
Language
Publication year range
1.
Mutat Res ; 409(1): 49-56, 1998 Oct 21.
Article in English | MEDLINE | ID: mdl-9806502

ABSTRACT

Cells from Cockayne's syndrome (CS) patients are hypersensitive to the cytotoxic effects of UV-irradiation and are defective in transcription coupled repair (TCR). We have examined the mutagenic consequences of impaired TCR in the Chinese hamster cell line UV61, the rodent homologue of CS complementation group B. Analysis of the two major UV-induced photolesions, cyclobutane pyrimidine dimers (CPD) and pyrimidine 6-4 pyrimidone photoproducts (6-4 PP), revealed that repair of CPD from the transcribed strand was strongly reduced in UV61 cells, but repair of 6-4 PP was indistinguishable from that in wild-type hamster cells. UV-induced mutation induction was enhanced in UV61 compared to that observed in repair proficient cells. The spectrum of UV-induced base substitutions in UV61 was clearly different from that observed in wild-type hamster cells and resembled the spectrum previously observed in nucleotide excision repair deficient hamster cells. In UV61 cells a strong strand bias for mutation induction was found; assuming that premutagenic lesions occur at dipyrimidine sequences, 76% of the mutations could be attributed to lesions in the transcribed strand. These data strongly favour the hypothesis that defective TCR of CPD is responsible for the enhanced UV-induced mutagenesis in UV61 cells.


Subject(s)
Cockayne Syndrome/genetics , Cockayne Syndrome/metabolism , Mutagenesis , Pyrimidine Dimers/genetics , Pyrimidine Dimers/metabolism , Animals , Base Sequence , CHO Cells , Cell Survival/radiation effects , Cricetinae , DNA/genetics , DNA Repair/genetics , DNA Repair/radiation effects , Humans , Hypoxanthine Phosphoribosyltransferase/genetics , Pyrimidine Dimers/radiation effects , RNA/biosynthesis , Radiation Tolerance/genetics , Transcription, Genetic , Ultraviolet Rays
2.
Mutagenesis ; 12(6): 417-24, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9412994

ABSTRACT

The role of the nucleotide excision repair (NER) pathway in removal of DNA ethylation damage was investigated by means of hprt mutational spectra analysis in the NER-deficient Chinese hamster ovary cell line UV5, which lacks ERCC2/XPD, and in its parental cell line AA8. Both cell lines were exposed to ethyl methanesulfonate (EMS) or N-ethyl-N-nitrosourea (ENU). EMS gave a similar dose-dependent increase in hprt mutants in UV5 compared with AA8. In both cell lines EMS-induced mutations in the hprt coding region consisted almost exclusively of GC-->AT transitions, probably due to the direct miscoding lesion O6-ethylguanine. ENU, an agent that in addition to O6-ethylguanine also induces other O-alkylation products, was significantly more mutagenic in UV5 than in AA8. Mutational spectra analysis showed that the proportions of ENU-induced GC-->AT, AT-->TA and AT-->GC base pair changes were similar for both cell lines. ENU-induced DNA lesions that may be involved in GC-->AT transitions are O6-ethylguanine and O2-ethylcytosine, the latter being a chemically stable DNA lesion of which the miscoding properties and repair characteristics are largely unknown. ENU-induced AT-->TA transversions are probably caused by O2-ethylthymine, which mispairs with thymine. In AA8 thymines in ENU-induced AT-->TA transversions were exclusively located in the non-transcribed strand of the hprt gene, whereas in UV5 30% of these thymines were found in the transcribed strand. Together, these results indicate that O6-ethylguanine is a poor substrate for NER in rodent cells and that O2-ethylpyrimidines are preferentially removed from the transcribed strand of the hprt gene by NER.


Subject(s)
Alkylating Agents/toxicity , DNA Repair , Ethyl Methanesulfonate/toxicity , Ethylnitrosourea/toxicity , Genes/drug effects , Hypoxanthine Phosphoribosyltransferase/drug effects , Hypoxanthine Phosphoribosyltransferase/genetics , Mutagens/toxicity , Animals , CHO Cells , Cricetinae , DNA/drug effects , DNA Damage , DNA Mutational Analysis , Guanine/metabolism , Mutagenicity Tests , Mutation , Species Specificity , Thymine/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...