ABSTRACT
65,000 sheep skin cDNA clones were gridded in high density on to nylon membranes and screened for (CA)n and (GA)n repeat containing clones. 296 dinucleotide repeat-containing clones were identified with approximately 85% non-redundancy. Clones were single-pass 5' sequenced and we compared the Expressed Sequence Tag (EST) sequences to the Swiss-Prot database to ascertain their identity and/or putative function. We then aligned the ESTs against the human genomic sequence to determine the locations of human orthologous sequences. Finally, we developed a subset of polymorphic microsatellite markers and positioned them on the ovine linkage map.
Subject(s)
Gene Library , Microsatellite Repeats/genetics , Sheep, Domestic/genetics , Skin/chemistry , Skin/metabolism , Animals , Chromosome Mapping/methods , Chromosome Mapping/veterinary , Cloning, Molecular/methods , DNA, Complementary/genetics , Databases, Genetic , Dinucleotide Repeats/genetics , Expressed Sequence Tags , Genome, Human , HumansABSTRACT
A medium-density linkage map of the ovine genome has been developed. Marker data for 550 new loci were generated and merged with the previous sheep linkage map. The new map comprises 1093 markers representing 1062 unique loci (941 anonymous loci, 121 genes) and spans 3500 cM (sex-averaged) for the autosomes and 132 cM (female) on the X chromosome. There is an average spacing of 3.4 cM between autosomal loci and 8.3 cM between highly polymorphic [polymorphic information content (PIC) > or = 0.7] autosomal loci. The largest gap between markers is 32.5 cM, and the number of gaps of > 20 cM between loci, or regions where loci are missing from chromosome ends, has been reduced from 40 in the previous map to 6. Five hundred and seventy-three of the loci can be ordered on a framework map with odds of > 1000 : 1. The sheep linkage map contains strong links to both the cattle and goat maps. Five hundred and seventy-two of the loci positioned on the sheep linkage map have also been mapped by linkage analysis in cattle, and 209 of the loci mapped on the sheep linkage map have also been placed on the goat linkage map. Inspection of ruminant linkage maps indicates that the genomic coverage by the current sheep linkage map is comparable to that of the available cattle maps. The sheep map provides a valuable resource to the international sheep, cattle, and goat gene mapping community.
Subject(s)
Chromosome Mapping/methods , Genetic Linkage , Genome , Sheep/genetics , Animals , Cattle , Female , Genetic Markers/genetics , Genotype , Male , Meiosis/genetics , Microsatellite Repeats/genetics , Minisatellite Repeats/genetics , Polymorphism, Restriction Fragment LengthSubject(s)
Anion Exchange Protein 1, Erythrocyte/genetics , Birds/genetics , Chromosome Mapping , Growth Hormone/genetics , Human Growth Hormone/genetics , Mammals/genetics , Polymorphism, Genetic , Animals , Base Sequence , Chickens/genetics , Conserved Sequence , Female , Genetic Carrier Screening , Genetic Linkage , Humans , Male , Mice/genetics , PedigreeABSTRACT
In work aimed at extending the chicken genome linkage map, a heat shock protein gene, HSP108, was shown by restriction fragment length polymorphism (RFLP) analysis to be linked to one of two replication histone gene clusters located on chromosome 1. Linkage was estimated using the LOD score method, on segregation data from seven pedigreed three-generation families (245 individuals). The maximum likelihood estimate of the recombination fraction (theta) was 0.215.
