ABSTRACT
In this experimental study, the influence of surgery-induced proinflammatory cytokines on tumor recurrence in the lung was investigated. A reproducible human in vitro assay was developed to study the adhesion of HT29 colon carcinoma cells to monolayers of microvascular endothelial cells of the lung (HMVECs-L) or human umbilical venous endothelial cells (HUVECs). Preincubation of HMVECs-L with maximally active concentrations of IL-1beta and TNF-alpha, but not with IL-6, resulted in at least 250% adhesion compared to control adhesion (p Subject(s)
Carcinoma/metabolism
, Cell Adhesion Molecules/metabolism
, Colonic Neoplasms/metabolism
, Endothelial Cells/metabolism
, Interleukin-1/metabolism
, Peptide Fragments/metabolism
, Tumor Necrosis Factor-alpha/metabolism
, Cell Adhesion
, Cell Line, Tumor
, Cell Proliferation
, Dose-Response Relationship, Drug
, E-Selectin/metabolism
, Gene Expression Regulation, Neoplastic
, Humans
, Immunoenzyme Techniques
, Immunohistochemistry
, In Vitro Techniques
, Inflammation
, Intercellular Adhesion Molecule-1/metabolism
, Interleukin-1beta
, Interleukin-6/metabolism
, Time Factors
, Vascular Cell Adhesion Molecule-1/metabolism
