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1.
Leukemia ; 22(7): 1387-94, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18418406

ABSTRACT

Mismatching for human leukocyte antigen (HLA)-DPB1 in unrelated donor hematopoietic stem cell transplantation (URD-SCT) has been associated with a decreased risk of disease relapse, indicating that HLA-DP may represent a target for graft-versus-leukemia (GVL) reactivity in HLA class II-expressing hematological malignancies. To investigate whether HLA-DP-specific T cells could mediate GVL reactivity following HLA-DPB1-mismatched URD-SCT and donor lymphocyte infusion (DLI), we analyzed the immune response in a patient with leukemic lymphoplasmacytic lymphoma responding to DLI without graft-versus-host disease. The emergence of leukemia-reactive CD4+ T cells during the clinical immune response was demonstrated by interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot(ELISPOT)analysis. Following clonal isolation of these leukemia-reactive CD4+ T cells, blocking studies, panel studies and retroviral transduction experiments of both mismatched HLA-DPB1 alleles identified HLA-DPB1(*)0201 and HLA-DPB1(*)0301 as the targets of this immune response. The HLA-DPB1-specific CD4+ T-cell clones were capable of recognizing and lysing several HLA-DP-expressing myeloid and lymphoid hematological malignant cells. Since HLA-DP expression is mainly restricted to hematopoietic cells, HLA-DP may be used as a specific target for immunotherapy following T-cell-depleted URD-SCT. Therefore, in patients with HLA class II-expressing hematological malignancies HLA-DP-mismatched SCT may be preferable over fully matched SCT allowing DLI to induce a GVL effect.


Subject(s)
HLA-DP Antigens/immunology , Hematopoietic Stem Cell Transplantation , Histocompatibility Antigens Class II/analysis , Immunotherapy, Adoptive , Leukemia, Lymphocytic, Chronic, B-Cell/therapy , CD4-Positive T-Lymphocytes/immunology , Female , Graft vs Host Disease/etiology , Graft vs Leukemia Effect , HLA-DP beta-Chains , Histocompatibility Testing , Humans , Leukemia, Lymphocytic, Chronic, B-Cell/immunology , Middle Aged
3.
Hum Immunol ; 67(11): 874-83, 2006 Nov.
Article in English | MEDLINE | ID: mdl-17145367

ABSTRACT

Low alloreactivity of umbilical cord blood (UCB) T-cells may explain diminished graft-versus-host-disease after UCB transplantation. We investigated whether UCB T-cells have an intrinsic lower capacity to become activated. T-cells from UCB or adult blood (AB) were stimulated with anti-CD3 and anti-CD28 antibodies. On days 1-3 after stimulation, T-cell activation was determined by CD25 expression, proliferation was measured, and kinetics of cell division were analyzed by staining with CFSE. UCB and AB T cells exhibited similar numbers of activated and proliferating cells, but the extent of activation was lower in UCB T-cells. Enzyme-linked immunospot analysis showed lower levels and slower kinetics of IL-2, IL-4, IL-10, and IFN-gamma secreting cells for UCB T-cells. Comparison of UCB T-cells with CD45RA+ naive or CD45RO+ memory T cells purified from AB showed relatively low numbers of IL-4 and IL-10 secreting T cells in CD45RA+ AB T-cells and UCB T-cells as compared with CD45RO+ AB T cells. Numbers of IL-2 or IFN-gamma secreting cells in adult CD45RA+ T-cells were lower than in CD45RO+ T-cells but higher than in UCB T-cells. Thus diminished reactivity of UCB T-cells was not caused by a lower capacity to become activated and proliferate but may be explained by a lower extent of activation in UCB T cells, the absence of memory T cells in UCB, and differences between naive T cells from UCB and AB.


Subject(s)
Cytokines/biosynthesis , Immunologic Memory/physiology , Interleukin-2 Receptor alpha Subunit , Lymphocyte Activation/immunology , T-Lymphocytes/immunology , Adult , Antibodies/immunology , CD28 Antigens/immunology , CD3 Complex/immunology , Cell Proliferation , Fetal Blood , Humans , Interleukin-2 Receptor alpha Subunit/metabolism , Kinetics , Leukocyte Common Antigens/metabolism , Leukocytes, Mononuclear
4.
Leukemia ; 20(6): 1040-6, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16525495

ABSTRACT

Although donor lymphocyte infusion (DLI) induces complete remissions in 70% of patients with relapsed chronic myeloid leukemia (CML) after allogeneic stem-cell transplantation (SCT), some patients are refractory to DLI by showing disease persistence. In a patient who received DLI for relapsed CML, we observed persisting molecular disease despite a hematological and cytogenetic remission in the absence of graft-versus-host disease (GVHD). To determine the nature of this immune response, we isolated leukemia-reactive donor T-cell clones from the bone marrow (BM) of the patient at the time of clinical response. Four different types of CD8+ HLA class I restricted T-cell clones were obtained that were cytotoxic against Ebstein-Barr virus-transformed B-cell lines (EBV-LCL) of the patient, but not the donor, indicating recognition of minor histocompatibility antigens (mHags). By using survival studies with CFSE labelled BM cells populations, a hematopoietic progenitor cell inhibition assay and direct morphological examination we showed that the T-cell clones recognized mature monocytic and myeloid cells, whereas immature BM progenitor cells were insufficiently lysed. This patient's refractoriness for DLI appears to be caused by inadequate lysis of progenitor cells by these cytotoxic T cells. These findings support the hypothesis that for eradication of CML a cytotoxic T-cell response against leukemic progenitor cells is essential.


Subject(s)
Antigens, Differentiation/immunology , Hematopoietic Stem Cell Transplantation/adverse effects , Hematopoietic Stem Cells/immunology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/immunology , Leukemia, Myeloid, Chronic-Phase/immunology , Neoplasm, Residual/immunology , T-Lymphocytes, Cytotoxic/immunology , Adult , Antigens, Differentiation/biosynthesis , CD8-Positive T-Lymphocytes/immunology , Cell Lineage/immunology , Drug Resistance, Neoplasm , Humans , Lymphocyte Transfusion/methods , Male , Neoplasm, Residual/genetics , T-Lymphocytes, Cytotoxic/transplantation , Transplantation, Homologous
5.
Leukemia ; 19(1): 83-90, 2005 Jan.
Article in English | MEDLINE | ID: mdl-15526024

ABSTRACT

Graft-vs-leukemia reactivity after donor lymphocyte infusion (DLI) can be mediated by donor T cells recognizing minor histocompatibility antigens (mHags) on recipient hematopoietic cells. To study the diversity of cells involved in this immune response, hematopoietic cell reactive T cells were directly clonally isolated from peripheral blood of patients entering complete remission after DLI. T cells were briefly stimulated with bone marrow cells from patients pretransplant, and IFNgamma-secreting T cells were directly clonally isolated, and expanded. Cytotoxic T-lymphocyte (CTL) clones from individual patients used multiple distinct HLA-restricting molecules and varied in reactivity against patient-derived normal and/or malignant hematopoietic cells. For each patient, CTL clones specific for known immunodominant mHags as well as distinct unknown mHags were found. Within individual patients, CTL clones using the same HLA-restricting element could show differential recognition patterns, indicating further diversity in mHag reactivity. CTL clones from individual patients exhibiting identical specificities could show oligoclonal origin. In conclusion, the direct cloning technique shows that the response to hematopoietic cells after DLI is directed against multiple distinct mHags, including but not limited to known immunodominant mHags, implying that immunotherapy with T cells against multiple mHag specificities may be more effective in eradicating malignant cells.


Subject(s)
Cell Transplantation , Interferon-gamma/metabolism , Major Histocompatibility Complex , T-Lymphocytes/immunology , Follow-Up Studies , Humans , Recurrence , T-Lymphocytes/metabolism , T-Lymphocytes/transplantation
6.
Hum Immunol ; 65(4): 328-39, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15120187

ABSTRACT

Because a relatively low incidence and severity of graft-versus-host disease after umbilical cord blood (UCB) transplantation is observed, we investigated whether T cells from UCB or adult blood (AB) were differentially activated by antigen-presenting cells with or without human leukocyte antigen (HLA)-DR expression. T cells from UCB or AB, or CD45RA(+) naive T cells and CD45RO(+) memory T cells separated from AB, were stimulated with the HLA-DR(+) or HLA-DR(-) cell line AML193. On days 1-3 after stimulation, numbers of interleukin (IL)-2, IL-4, IL-10 or interferon gamma (IFN-gamma)-secreting cells were determined by enzyme-linked immunospot analysis. No IL-4 or IL-10 was produced. AML193-DR(+) cells induced IL-2 and IFN-gamma secretion with slower kinetics and lower levels in UCB T cells than in AB T cells. AML193-DR(+) cells induced comparable IL-2 but higher IFN-gamma secretion in CD45RA(+) T cells from AB than in UCB T cells. AML193-DR(-) cells did not induce IL-2- or IFN-gamma secretion in UCB T cells, but stimulated both CD45RA(+) and CD45RO(+) T cells from AB to secrete IL-2 and IFN-gamma. Thus, not only the absence of memory T cells but also the inability to respond to HLA-DR-negative antigen-presenting cells and the slower kinetics and level of activation found for naive T cells from UCB as compared with AB may partly explain the reduced antirecipient reactivity after UCB transplantation.


Subject(s)
Antigen-Presenting Cells/immunology , Cytokines/biosynthesis , Fetal Blood/immunology , HLA Antigens/metabolism , Histocompatibility Antigens Class II/metabolism , T-Lymphocytes/immunology , Antigens, CD/immunology , Antigens, Differentiation, T-Lymphocyte/immunology , Cell Line , Fetal Blood/cytology , HLA-A Antigens/immunology , HLA-D Antigens/immunology , Humans , Interferon-gamma/biosynthesis , Interleukin-2/biosynthesis , Isoantigens/immunology , Lectins, C-Type , Receptors, Interleukin-2/immunology
7.
Leukemia ; 18(4): 798-808, 2004 Apr.
Article in English | MEDLINE | ID: mdl-14973499

ABSTRACT

Donor T cells recognizing hematopoiesis-restricted minor histocompatibility antigens (mHags) HA-1 and HA-2 on malignant cells play a role in the antileukemia effect of donor lymphocyte infusion (DLI) in patients with relapsed leukemia after allogeneic stem cell transplantation. We quantified the contribution of HA-1 and HA-2 specific T cells to the total number of leukemia-reactive T cells in three HA-2 and/or HA-1 positive patients responding to DLI from their mHag negative donors. Clinical responses occurring 5-7 weeks after DLI were accompanied by an increase in percentages HLA-DR expressing T cells within the CD8+ T cell population. To clonally analyze the leukemia-reactive immune response, T cells responding to the malignancy by secreting IFNgamma were isolated from peripheral blood, directly cloned, and expanded. Tetramer analysis and specific lysis of peptide-pulsed target cells showed that 3-35% of cytotoxic T lymphocyte (CTL) clones isolated were specific for HA-1 or HA-2. TCR VB analysis showed oligoclonal origin of the HA-1 and HA-2 specific CTL clones. The HA-1 and HA-2 specific CTL clones inhibited leukemic progenitor cell growth in vitro. The relatively high frequency of HA-1 and HA-2 specific T cells within the total number of tumor-reactive T cells illustrates relative immunodominance of mHags HA-1 and HA-2.


Subject(s)
Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy , Lymphocyte Transfusion/methods , T-Lymphocytes/cytology , T-Lymphocytes/immunology , Cell Culture Techniques , Clone Cells/cytology , Clone Cells/immunology , Cytotoxicity, Immunologic , Female , Graft vs Leukemia Effect , HLA-DR Antigens/analysis , Hematopoiesis , Humans , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/immunology , Male , Minor Histocompatibility Antigens/immunology , Multiple Myeloma/immunology , Multiple Myeloma/therapy , Neoplasm Proteins/immunology , Oligopeptides/immunology , Salvage Therapy/methods , T-Lymphocytes/transplantation , T-Lymphocytes, Cytotoxic/cytology , T-Lymphocytes, Cytotoxic/immunology , Transplantation, Homologous
8.
Methods ; 31(2): 113-9, 2003 Oct.
Article in English | MEDLINE | ID: mdl-12957568

ABSTRACT

Adoptive immunotherapy with donor T lymphocytes may be used as a treatment for relapsed leukemia after allogeneic hematopoietic stem cell transplantation (SCT). In vitro selected and expanded anti-leukemic T cells may be more effective in inducing a response in vivo. To identify the anti-leukemic reactivity of in vitro generated T cells, standard target cell read-out assays like the 51Cr-release assay are not always appropriate. We developed an assay in which the ability of T cells to antigen specifically inhibit the in vitro growth of leukemic progenitor cells in the presence of cytokines can be measured. This assay allows the evaluation of the cytolytic or suppressive potential of leukemia reactive T cells for prolonged periods of time. The assay is based on inhibition of [3H]thymidine incorporation by the leukemic progenitor cells induced by multiple hematopoietic growth factors. T cell clones with a known specificity were used to compare the analytic potential of the new assay with those of other cytotoxicity assays. Based on the results of the T cell clones, a modification of a limiting dilution assay was developed to identify anti-leukemic allogeneic T cells in HLA identical donor-recipient combinations selected on their ability to inhibit the in vitro growth of CML or AML progenitor cells, to be used for the generation of leukemia-reactive CTL lines for clinical use.


Subject(s)
Immunotherapy, Adoptive/methods , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/immunology , Leukemia, Myeloid/immunology , T-Lymphocytes, Cytotoxic/immunology , Acute Disease , Antigens, CD34/immunology , Cell Division/drug effects , Clone Cells , Colony-Forming Units Assay , Cytokines/immunology , Cytotoxicity, Immunologic , Dose-Response Relationship, Drug , Humans , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy , Leukemia, Myeloid/pathology , Leukemia, Myeloid/therapy , Neoplastic Stem Cells/immunology , Neoplastic Stem Cells/pathology , Thymidine/metabolism , Tumor Cells, Cultured
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