1.
J Chromatogr B Analyt Technol Biomed Life Sci
; 852(1-2): 631-4, 2007 Jun 01.
Article
in English
| MEDLINE
| ID: mdl-17321806
ABSTRACT
An LC-MS/MS method has been developed for the quantitative determination of a protein drug (Tenecteplase; M(W) 58,777 Da) in rat plasma. The protein was digested with trypsin without prior clean-up of the plasma sample, without the use of a label nor internal standard. A limited validation was performed to assess the linearity, the sensitivity and the specificity of the method. In addition, the developed method was applied to the quantitative analysis of Tenecteplase in rat plasma samples originating from a single-dose study in rats.