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1.
Cancer Immunol Immunother ; 67(10): 1505-1518, 2018 Oct.
Article in English | MEDLINE | ID: mdl-30039426

ABSTRACT

In elderly acute myeloid leukemia (AML) patients post-remission treatment options are associated with high comorbidity rates and poor survival. Dendritic cell (DC)-based immunotherapy is a promising alternative treatment strategy. A novel allogeneic DC vaccine, DCP-001, was developed from an AML-derived cell line that uniquely combines the positive features of allogeneic DC vaccines and expression of multi-leukemia-associated antigens. Here, we present data from a phase I study conducted with DCP-001 in 12 advanced-stage elderly AML patients. Patients enrolled were in complete remission (CR1/CR2) (n = 5) or had smoldering disease (n = 7). All patients were at high risk of relapse and ineligible for post-remission intensification therapies. A standard 3 + 3 dose escalation design with extension to six patients in the highest dose was performed. Patients received four biweekly intradermal DCP-001 injections at different dose levels (10, 25, and 50 million cells DCP-001) and were monitored for clinical and immunological responses. Primary objectives of the study (feasibility and safety) were achieved with 10/12 patients completing the vaccination program. Treatment was well tolerated. A clear-cut distinction between patients with and without detectable circulating leukemic blasts during the vaccination period was noted. Patients with no circulating blasts showed an unusually prolonged survival [median overall survival 36 months (range 7-63) from the start of vaccination] whereas patients with circulating blasts, died within 6 months. Long-term survival was correlated with maintained T cell levels and induction of multi-functional immune responses. It is concluded that DCP-001 in elderly AML patients is safe, feasible and generates both cellular and humoral immune responses.


Subject(s)
Cancer Vaccines/immunology , Dendritic Cells/immunology , Immunotherapy , Leukemia, Myeloid, Acute/prevention & control , T-Lymphocytes/immunology , Aged , Cancer Vaccines/administration & dosage , Female , Humans , Leukemia, Myeloid, Acute/immunology , Male , Middle Aged , Remission Induction , Treatment Outcome
2.
J Immunother ; 40(9): 315-322, 2017.
Article in English | MEDLINE | ID: mdl-28961609

ABSTRACT

Multiple myeloma (MM) is characterized by progressive immune dysregulation, loss of myeloma-specific immunity, and an immunosuppressive milieu that fosters disease growth and immune escape. Accordingly, cancer vaccines that reverse tumor-associated immune suppression represent a promising therapeutic avenue of investigation. We examined the potential of an allogeneic cellular vaccine to generate immune responses against MM tumor cells. The DCOne vaccine is comprised of a human myeloid leukemia cell line differentiated into a fully functional dendritic cell, expressing a range of tumor-associated antigens that are also known targets in MM. We found that the myeloma-specific antigens expressed by the DCOne vaccine can traffic via extracellular vesicles to surrounding antigen-presenting cells, thus stimulating autologous T-cell responses. Indeed, coculture of peripheral blood mononuclear cells from patients with MM with the DCOne vaccine resulted in the expansion of activated CD8 T cells expressing interferon-γ and perforin, with no significant change in the percentage of CD4 T cells producing interleukin-10. Further, coculture of patient's tumor cells with peripheral blood mononuclear cells and DCOne induced cytotoxic T-lymphocyte-mediated killing of autologous MM cells. These findings demonstrate that the allogeneic DCOne vaccine can induce T-cell activation and myeloma-specific immunity via cross presentation of antigens by native antigen-presenting cells.


Subject(s)
Antigens, Neoplasm/immunology , CD8-Positive T-Lymphocytes/immunology , Dendritic Cells/immunology , Extracellular Vesicles/immunology , Immunotherapy, Adoptive/methods , Multiple Myeloma/therapy , Cancer Vaccines , Cell Differentiation , Cell Line, Tumor , Coculture Techniques , Cross-Priming , Cytotoxicity, Immunologic , Dendritic Cells/transplantation , Humans , Interferon-gamma/metabolism , Isoantigens/immunology , Lymphocyte Activation , Multiple Myeloma/immunology , Perforin/metabolism , Tumor Microenvironment
3.
Immunotherapy ; 5(11): 1183-90, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24188673

ABSTRACT

AIMS: Vaccination with acute myeloid leukemia (AML)-derived dendritic cells (DCs) is a promising immunotherapeutic approach to prevent relapse of AML. However, in clinical practice AML-derived DC culture is unfeasible in 40% of cases. Here, we demonstrate that AML cells can be expanded in vitro prior to differentiation with cocktails of cytokines with known myeloid growth-promoting effects. RESULTS: Nine out of 13 initially CD14(-) samples gain de novo CD14 (>10%) expression (69% increment; p = 0.01) after in vitro expansion. These expanded CD14(+) leukemic cells displayed a high probability (six out of six initially CD14(-) samples tested) to differentiate into DCs upon culture with GM-CSF, TNF-α and IL-4. CONCLUSION: Induction of CD14 on initially CD14(-) AML cells potentially increases the number of patients eligible for DC-based immunotherapy.


Subject(s)
Dendritic Cells , Leukemia, Myeloid, Acute , Lipopolysaccharide Receptors/immunology , Vaccination , Cell Line, Tumor , Cytokines/immunology , Cytokines/pharmacology , Dendritic Cells/immunology , Dendritic Cells/transplantation , Female , Humans , Leukemia, Myeloid, Acute/immunology , Leukemia, Myeloid, Acute/therapy , Male
4.
Respir Med ; 107(7): 959-66, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23639272

ABSTRACT

BACKGROUND: Smoking in asthma occurs frequently and is associated with increased symptom severity, an impaired response to corticosteroids, and accelerated lung function decline. Airway pathology in smoking asthmatics is characterized by neutrophilia and epithelial changes such as goblet cell hyperplasia and increased proliferation. Bronchial CD8(+) T cells are implicated in lung function decline in asthma and COPD. We hypothesized that smoking modifies airway inflammation in asthma by increasing the number of CD8(+) T cells at an early stage. OBJECTIVES & METHODS: To study effects of smoking on airway pathology in bronchial biopsies from atopic patients with controlled intermittent or mild persistent asthma (12 smokers, 9.7 py and 11 never-smokers, 0.0 py; 20-50 yrs; FEV1 > 70% predicted; PC20MCh < 8 mg/mL, no ICS) using immunohistochemistry. RESULTS: Smoking asthmatics showed higher numbers of bronchial CD8(+) T cells (55.8 vs 23.9 cells/0.1 mm(2); p = 0.001) and CD68(+) macrophages (7.5 vs 4.6 cells/0.1 mm(2), p = 0.012), and a lower CD4(+)/CD8(+) cell ratio (0.16 vs 0.40; p = 0.007) compared with non-smoking asthmatics, but no difference in neutrophils. Furthermore, the % intact epithelium was higher in smoking asthmatics (49.3 vs 23.3, p = 0.001). CONCLUSION: Smoking asthmatics with a limited smoking history show a distinct pattern of airway pathology characterized by a bronchial infiltrate of CD8(+) T cells and CD68(+) macrophages, and epithelial remodelling resembling COPD-like features. This raises the hypothesis that early presence of CD8(+) T cells contributes to disease progression in smoking asthmatics.


Subject(s)
Asthma/etiology , Asthma/immunology , CD8-Positive T-Lymphocytes/pathology , Smoking/adverse effects , Adult , Asthma/pathology , Asthma/physiopathology , Biopsy , Bronchi/immunology , Bronchi/pathology , Bronchitis/etiology , Bronchitis/immunology , Bronchitis/pathology , Bronchoscopy/methods , CD4-CD8 Ratio , Cross-Sectional Studies , Disease Progression , Female , Forced Expiratory Volume/physiology , Humans , Lymphocyte Count , Male , Middle Aged , Respiratory Mucosa/immunology , Respiratory Mucosa/pathology , Smoking/immunology , Smoking/physiopathology , Young Adult
5.
Cancer Immunol Immunother ; 61(2): 181-191, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21874304

ABSTRACT

Appropriate activation of dendritic cells (DC) is essential for successful active vaccination and induction of cell-mediated immunity. The scarcity of precursor cells, as well as long culture methods, have hampered wide-scale application of DC vaccines derived from CD34(+) precursors, despite their suggested superior efficacy over the more commonly applied monocyte-derived DC (MoDC). Here, employing the CD34(+)/CD14(+) AML-derived human DC progenitor cell line MUTZ3, we show that cytostatic anthraquinone-derivatives (i.e., the anthracenedione mitoxantrone and the related anthracyclin doxorubicin) induce rapid differentiation of CD34(+) DC precursors into functional antigen-presenting cells (APC) in a three-day protocol. The drugs were found to act specifically on CD34(+), and not on CD14(+) DC precursors. Importantly, these observations were confirmed for primary CD34(+) and CD14(+) DC precursors from peripheral blood. Mitoxantrone-generated DC were fully differentiated within three days and after an additional 24 h of maturation, were as capable as standard 9-day differentiated and matured DC to migrate toward the lymph node-homing chemokines CCL19 and CCL21, to induce primary allogeneic T cell proliferation, and to prime functional MART1-specific CD8(+) T lymphocytes. Our finding that anthraquinone-derivatives like mitoxantrone support rapid high-efficiency differentiation of DC precursors may have consequences for in vitro production of DC vaccines as well as for novel immunochemotherapy strategies.


Subject(s)
Cancer Vaccines , Dendritic Cells/metabolism , Immunotherapy , Myeloid Progenitor Cells/metabolism , Neoplasms/immunology , Anthracyclines/pharmacology , Anthraquinones/pharmacology , Antigens, CD34/metabolism , Antineoplastic Agents/pharmacology , Cell Differentiation , Cell Line , Chemokine CCL19/metabolism , Chemokine CCL21/metabolism , Cytostatic Agents/pharmacology , Dendritic Cells/immunology , Dendritic Cells/pathology , Dendritic Cells/transplantation , Humans , Immunity, Cellular , Lipopolysaccharide Receptors/metabolism , Myeloid Progenitor Cells/immunology , Myeloid Progenitor Cells/pathology , Neoplasms/therapy
6.
Respir Res ; 12: 59, 2011 May 02.
Article in English | MEDLINE | ID: mdl-21529380

ABSTRACT

The airway epithelium forms a barrier against infection but also produces antimicrobial peptides (AMPs) and other inflammatory mediators to activate the immune system. It has been shown that in allergic disorders, Th2 cytokines may hamper the antimicrobial activity of the epithelium. However, the presence of Th2 cytokines also affects the composition of the epithelial layer which may alter its function. Therefore, we investigated whether exposure of human primary bronchial epithelial cells (PBEC) to Th2 cytokines during mucociliary differentiation affects expression of the human cathelicidin antimicrobial protein (hCAP18)/LL-37 and human beta defensins (hBD), and antimicrobial activity.PBEC were cultured at an air-liquid interface (ALI) for two weeks in the presence of various concentrations of IL-4 or IL-13. Changes in differentiation and in expression of various AMPs and the antimicrobial proteinase inhibitors secretory leukocyte protease inhibitor (SLPI) and elafin were investigated as well as antimicrobial activity.IL-4 and IL-13 increased mRNA expression of hCAP18/LL-37 and hBD-2. Dot blot analysis also showed an increase in hCAP18/LL-37 protein in apical washes of IL-4-treated ALI cultures, whereas Western Blot analysis showed expression of a protein of approximately 4.5 kDa in basal medium of IL-4-treated cultures. Using sandwich ELISA we found that also hBD-2 in apical washes was increased by both IL-4 and IL-13. SLPI and elafin levels were not affected by IL-4 or IL-13 at the mRNA or protein level. Apical wash obtained from IL-4- and IL-13-treated cultures displayed increased antimicrobial activity against Pseudomonas aeruginosa compared to medium-treated cultures. In addition, differentiation in the presence of Th2 cytokines resulted in increased MUC5AC production as has been shown previously.These data suggest that prolonged exposure to Th2 cytokines during mucociliary differentiation contributes to antimicrobial defence by increasing the expression and release of selected antimicrobial peptides and mucus.


Subject(s)
Antimicrobial Cationic Peptides/metabolism , Bronchi/metabolism , Cell Differentiation , Epithelial Cells/metabolism , Interleukin-13/metabolism , Interleukin-4/metabolism , Mucociliary Clearance , Respiratory Mucosa/metabolism , Antimicrobial Cationic Peptides/genetics , Blotting, Western , Bronchi/immunology , Bronchi/microbiology , Cathelicidins/metabolism , Cells, Cultured , Elafin/metabolism , Enzyme-Linked Immunosorbent Assay , Epithelial Cells/immunology , Epithelial Cells/microbiology , Humans , Mucin 5AC/metabolism , Pseudomonas aeruginosa/growth & development , Pseudomonas aeruginosa/metabolism , RNA, Messenger/metabolism , Respiratory Mucosa/immunology , Respiratory Mucosa/microbiology , Reverse Transcriptase Polymerase Chain Reaction , Secretory Leukocyte Peptidase Inhibitor/metabolism , Time Factors , beta-Defensins/metabolism
7.
Am J Pathol ; 176(4): 1648-59, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20150432

ABSTRACT

Ischemia-reperfusion injury is the major cause of delayed graft function in transplanted kidneys, an early event significantly affecting long-term graft function and survival. Several studies in rodents suggest that the alternative pathway of the complement system plays a pivotal role in renal ischemia-reperfusion injury. However, limited information is currently available from humans and larger animals. Here we demonstrated that 30 minutes of ischemia resulted in the induction of C4d/C1q, C4d/MLB, and MBL/MASP-2 deposits in a swine model of ischemia-reperfusion injury. The infusion of C1-inhibitor led to a significant reduction in peritubular capillary and glomerular C4d and C5b-9 deposition. Moreover, complement-inhibiting treatment significantly reduced the numbers of infiltrating CD163(+), SWC3a(+), CD4a(+), and CD8a(+) cells. C1-inhibitor administration led to significant inhibition of tubular damage and tubular epithelial cells apoptosis. Interestingly, we report that focal C4d-deposition colocalizes with C1q and MBL at the peritubular and glomerular capillary levels also in patients with delayed graft function. In conclusion, we demonstrated the activation and a pathogenic role of classical and lectin pathways of complement in a swine model of ischemia-reperfusion-induced renal damage. Therefore, inhibition of these two pathways might represent a novel therapeutic approach in the prevention of delayed graft function in kidney transplant recipients.


Subject(s)
Complement System Proteins/metabolism , Kidney Diseases/pathology , Lectins/chemistry , Reperfusion Injury/metabolism , Animals , Complement C1 Inhibitor Protein/biosynthesis , Complement C1q/metabolism , Complement C4b/metabolism , Disease Models, Animal , Female , Graft Survival , Humans , Immunohistochemistry/methods , Ischemia/pathology , Kidney Diseases/metabolism , Peptide Fragments/metabolism , Swine
8.
Mol Immunol ; 44(5): 803-11, 2007 Feb.
Article in English | MEDLINE | ID: mdl-16740309

ABSTRACT

The composition of the airway epithelium is dynamic and epithelial differentiation is regulated by endogenous mediators as well as inhaled substances. In atopic asthma the differentiation of the epithelium is altered. Various studies have addressed the ability of cultured airway epithelial cells to release the eosinophil-attractant chemokines eotaxin, eotaxin-2 and eotaxin-3 using epithelial cell lines or poorly differentiated primary cells. Since little is known about the role of the epithelial differentiation state in the response of epithelial cells to stimuli that increase production of mediators such as the eotaxins, we analyzed the effect of differentiation state on the production of the eotaxins. In particular, we investigated the effects of the Th2 cytokines IL-4 and IL-13 on eotaxin-2 and -3 production by primary human bronchial epithelial cells and examined whether their production is affected by epithelial cell differentiation using both submerged and air-liquid interface (ALI) cultures. The results show that both IL-4 and IL-13 increase eotaxin-2 and -3 mRNA expression and protein release in submerged- and ALI-cultures. Moreover, epithelial differentiation in ALI-cultures appeared an important determinant in the regulation of eotaxin-2 and -3. Mucociliary differentiation of the epithelial cells was induced by culture in the presence of a high concentration of retinoic acid (RA), whereas low concentrations of RA resulted in a flattened squamous epithelial phenotype. Mucociliary differentiated ALI-cultures expressed and released more eotaxin-3 upon stimulation with IL-4/IL-13, whereas eotaxin-2 production was predominantly found in squamous differentiated ALI-cultures. TNFalpha reduced IL-4-induced eotaxin-2 release in submerged cultures but not in ALI-cultures; no effects on eotaxin-3 synthesis were observed. The results indicate that epithelial differentiation is an important determinant in Th2 cytokine-induced eotaxin-2 and -3 release by airway epithelial cells. These findings may provide new insights into the role of airway epithelial differentiation and Th2 cytokines in the pathogenesis of inflammatory lung disorders such as asthma.


Subject(s)
Chemokines, CC/biosynthesis , Epithelial Cells/cytology , Interleukin-13/pharmacology , Interleukin-4/pharmacology , Bronchi/cytology , Cell Culture Techniques , Cell Differentiation/physiology , Cells, Cultured , Chemokine CCL24 , Chemokine CCL26 , Chemokines, CC/genetics , Epithelial Cells/drug effects , Epithelial Cells/metabolism , Humans , RNA, Messenger/biosynthesis , Th2 Cells/immunology , Tumor Necrosis Factor-alpha/pharmacology
9.
Respir Res ; 6: 140, 2005 Nov 25.
Article in English | MEDLINE | ID: mdl-16309548

ABSTRACT

BACKGROUND: Increased airway epithelial proliferation is frequently observed in smokers. To elucidate the molecular mechanisms leading to these epithelial changes, we studied the effect of cigarette smoke condensate (CSC) on cell proliferation, wound closure and mitogen activated protein kinase (MAPK) activation. We also studied whether modulation of intracellular glutathione/thiol levels could attenuate CSC-induced cell proliferation. METHODS: Cells of the bronchial epithelial cell line NCI-H292 and subcultures of primary bronchial epithelial cells were used for the present study. The effect of CSC on epithelial proliferation was assessed using 5-bromo-2-deoxyuridine (BrdU) incorporation. Modulation of epithelial wound repair was studied by analysis of closure of 3 mm circular scrape wounds during 72 hours of culture. Wound closure was calculated from digital images obtained at 24 h intervals. Activation of mitogen-activated protein kinases was assessed by Western blotting using phospho-specific antibodies. RESULTS: At low concentrations CSC increased proliferation of NCI-H292 cells, whereas high concentrations were inhibitory as a result of cytotoxicity. Low concentrations of CSC also increased epithelial wound closure of both NCI-H292 and PBEC, whereas at high concentrations closure was inhibited. At low, mitogenic concentrations, CSC caused persistent activation of ERK1/2, a MAPK involved in cell proliferation. Inhibition of cell proliferation by high concentrations of CSC was associated with activation of the pro-apoptotic MAP kinases p38 and JNK. Modulation of intracellular glutathione (GSH)/thiol levels using N-acetyl-L-cysteine, GSH or buthionine sulphoximine (BSO), demonstrated that both the stimulatory and the inhibitory effects of CSC were regulated in part by intracellular GSH levels. CONCLUSION: These results indicate that CSC may increase cell proliferation and wound closure dependent on the local concentration of cigarette smoke and the anti-oxidant status. These findings are consistent with increased epithelial proliferation in smokers, and may provide further insight in the development of lung cancer.


Subject(s)
Bronchi/metabolism , Bronchi/pathology , Epithelial Cells/metabolism , Epithelial Cells/pathology , Glutathione/metabolism , Tars/pharmacology , Wound Healing/drug effects , Bronchi/drug effects , Cell Proliferation/drug effects , Cells, Cultured , Dose-Response Relationship, Drug , Epithelial Cells/drug effects , Humans , MAP Kinase Signaling System/drug effects , Reactive Oxygen Species/metabolism
10.
J Leukoc Biol ; 77(4): 444-50, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15591123

ABSTRACT

Most antimicrobial peptides have been discovered based on activity-guided purification procedures, which used assays to determine their antimicrobial activity. Nevertheless, recent studies have shown that antimicrobial peptides also exert a range of other functions. Based on these observations, antimicrobial peptides are now not only implicated in host defense against infection but also in other immune reactions, inflammation, and wound-repair processes. The activities of neutrophil defensins and the cathelicidin hCAP-18/LL-37, antimicrobial peptides that are abundantly expressed in the human neutrophil, are the subject of an increasing number of studies. Exposure to neutrophil defensins and hCAP-18/LL-37 results in increases in mediator expression and release, chemotaxis, and proliferation of inflammatory and epithelial cells and fibroblasts, and the mechanisms underlying these effects have been partly elucidated. This review is focused on the effects of neutrophil defensins and hCAP-18/LL-37 on airway epithelial cells.


Subject(s)
Antimicrobial Cationic Peptides/physiology , Lung Diseases/physiopathology , Neutrophils/physiology , Respiratory Mucosa/physiology , Anticarcinogenic Agents , Humans , Inflammation , Cathelicidins
11.
Am J Respir Cell Mol Biol ; 30(2): 193-201, 2004 Feb.
Article in English | MEDLINE | ID: mdl-12871849

ABSTRACT

Human airways are frequently exposed to potentially harmful agents that cause tissue injury. Upon such injury, a repair process is initiated that comprises cell migration, proliferation, and differentiation. We have previously shown that human neutrophil defensins (human neutrophil peptides 1-3 [HNP1-3]) induce airway epithelial cell proliferation. Because of the role of cell proliferation in epithelial wound repair, we investigated the effect of HNP1-3 on airway epithelial wound closure and mucin gene expression in vitro. Using NCI-H292 airway epithelial cell cultures, we demonstrated that HNP1-3 cause a dose- and time-dependent increase of wound closure as well as increased cell migration. Furthermore, HNP1-3 caused a biphasic activation of the mitogen-activated protein kinase extracellular-regulated kinase 1 and 2 (ERK1/2). Both the effects of HNP1-3 on wound closure and ERK1/2 activation were blocked by specific inhibitors of the mitogen-activated protein kinase kinase MEK, whereas inhibitors of epidermal growth factor receptor tyrosine kinase, phosphatidylinositol 3-kinase, and Src did block defensin-enhanced wound closure but not ERK1/2 activation. Finally, HNP1-3 increased mRNA encoding the mucins MUC5B and MUC5AC, suggesting a role for defensins in mucous cell differentiation. These results indicate that neutrophil defensins increase epithelial wound repair in vitro, which involves migration and proliferation, and mucin production. Neutrophil defensin-enhanced wound repair appears to require epidermal growth factor receptor activation and downstream signaling pathways.


Subject(s)
Anti-Infective Agents/metabolism , Epithelial Cells/metabolism , Mucins/metabolism , Neutrophils/metabolism , Respiratory Mucosa/pathology , alpha-Defensins/metabolism , Cell Line , Cell Movement , Enzyme Activation , Epithelial Cells/cytology , ErbB Receptors/metabolism , Humans , MAP Kinase Signaling System/physiology , Mitogen-Activated Protein Kinases/metabolism , Mucins/genetics , Respiratory Mucosa/cytology
12.
Am J Physiol Cell Physiol ; 285(2): C343-52, 2003 Aug.
Article in English | MEDLINE | ID: mdl-12700137

ABSTRACT

Leukocyte adhesion is mediated totally and transendothelial migration partially by heterotypic interactions between the beta1- and beta2-integrins on the leukocytes and their ligands, Ig-like cell adhesion molecules (Ig-CAM), VCAM-1, and ICAM-1, on the endothelium. Both integrins and Ig-CAMs are known to have signaling capacities. In this study we analyzed the role of VCAM-1-mediated signaling in the control of endothelial cell-cell adhesion and leukocyte transendothelial migration. Antibody-mediated cross-linking of VCAM-1 on IL-1beta-activated primary human umbilical vein endothelial cells (pHUVEC) induced actin stress fiber formation, contractility, and intercellular gaps. The effects induced by VCAM-1 cross-linking were inhibited by C3 toxin, indicating that the small GTPase p21Rho is involved. In addition, the effects of VCAM-1 were accompanied by activation of Rac, which we recently showed induce intercellular gaps in pHUVEC in a Rho-dependent fashion. With the use of a cell-permeable peptide inhibitor, it was shown that Rac signaling is required for VCAM-1-mediated loss of cell-cell adhesion. Furthermore, VCAM-1-mediated signaling toward cell-cell junctions was accompanied by, and dependent on, Rac-mediated production of reactive oxygen species and activation of p38 MAPK. In addition, it was found that inhibition of Rac-mediated signaling blocks transendothelial migration of monocytic U937 cells. Together, these data indicate that VCAM-1-induced, Rac-dependent signaling plays a key role in the modulation of vascular-endothelial cadherin-mediated endothelial cell-cell adhesion and leukocyte extravasation.


Subject(s)
Cell Adhesion/physiology , Cell Communication/physiology , Chemotaxis, Leukocyte/physiology , Endothelium, Vascular/metabolism , Leukocytes/metabolism , Vascular Cell Adhesion Molecule-1/metabolism , rac GTP-Binding Proteins/metabolism , ADP Ribose Transferases/pharmacology , Botulinum Toxins/pharmacology , Cell Adhesion/drug effects , Cell Communication/drug effects , Cell Line , Chemotaxis, Leukocyte/drug effects , Endothelium, Vascular/cytology , Endothelium, Vascular/drug effects , Enzyme Inhibitors/pharmacology , Humans , Interleukin-1/pharmacology , Leukocytes/cytology , Leukocytes/drug effects , Mitogen-Activated Protein Kinases/drug effects , Mitogen-Activated Protein Kinases/metabolism , Peptide Fragments/pharmacology , Reactive Oxygen Species/metabolism , Signal Transduction/drug effects , Signal Transduction/physiology , Vascular Cell Adhesion Molecule-1/drug effects , p38 Mitogen-Activated Protein Kinases , rac GTP-Binding Proteins/drug effects , rhoA GTP-Binding Protein/drug effects , rhoA GTP-Binding Protein/metabolism
13.
J Leukoc Biol ; 72(1): 167-74, 2002 Jul.
Article in English | MEDLINE | ID: mdl-12101277

ABSTRACT

Repair of injured airway epithelium is often accompanied by an influx of leukocytes, and these cells have been suggested to contribute to the repair process. The aim of the present study was to investigate the effect of neutrophil defensins--antimicrobial peptides present in large amounts in the neutrophil--on proliferation of cultured lung epithelial cells. Neutrophil defensins at 4-10 microg/ml enhanced proliferation of the A549 lung epithelial cell line as assessed using cell counting, BrdU incorporation, and the tetrazolium salt MTT assay. Higher, cytotoxic concentrations of defensins decreased cell proliferation. Whereas defensin-induced cell proliferation was not inhibited by the EGF receptor tyrosine kinase inhibitor AG1478, it was completely inhibited by the mitogen-activated protein (MAP) kinase kinase (MEK) inhibitor U0126, suggesting that defensins mediate cell proliferation via an EGF receptor-independent, MAP kinase signaling pathway. Although the cytotoxic effect of defensins was inhibited by alpha1-proteinase inhibitor, the defensin-induced cell proliferation was not affected. These data suggest that neutrophil defensins may possibly be involved in epithelial repair in the airways by inducing lung epithelial cell proliferation.


Subject(s)
Defensins/pharmacology , Lung/cytology , Respiratory Mucosa/cytology , Cell Division/drug effects , Defensins/isolation & purification , Dose-Response Relationship, Drug , Epithelial Cells/cytology , Epithelial Cells/drug effects , Epithelial Cells/metabolism , ErbB Receptors/physiology , Growth Substances/pharmacology , Humans , MAP Kinase Signaling System , Neutrophils/chemistry , Respiratory Mucosa/drug effects , Respiratory Mucosa/metabolism , Serine Proteinase Inhibitors/pharmacology , Tumor Cells, Cultured , alpha 1-Antitrypsin/pharmacology
14.
J Cell Sci ; 115(Pt 9): 1837-46, 2002 May 01.
Article in English | MEDLINE | ID: mdl-11956315

ABSTRACT

The integrity of the endothelium is dependent on cell-cell adhesion, which is mediated by vascular-endothelial (VE)-cadherin. Proper VE-cadherin-mediated homotypic adhesion is, in turn, dependent on the connection between VE-cadherin and the cortical actin cytoskeleton. Rho-like small GTPases are key molecular switches that control cytoskeletal dynamics and cadherin function in epithelial as well as endothelial cells. We show here that a cell-penetrating, constitutively active form of Rac (Tat-RacV12) induces a rapid loss of VE-cadherin-mediated cell-cell adhesion in endothelial cells from primary human umbilical veins (pHUVEC). This effect is accompanied by the formation of actin stress fibers and is dependent on Rho activity. However, transduction of pHUVEC with Tat-RhoV14, which induces pronounced stress fiber and focal adhesion formation, did not result in a redistribution of VE-cadherin or an overall loss of cell-cell adhesion. In line with this observation, endothelial permeability was more efficiently increased by Tat-RacV12 than by Tat-RhoV14. The loss of cell-cell adhesion, which is induced by Tat-RacV12, occurred in parallel to and was dependent upon the intracellular production of reactive oxygen species (ROS). Moreover, Tat-RacV12 induced an increase in tyrosine phosphorylation of a component the VE-cadherin-catenin complex, which was identified as alpha-catenin. The functional relevance of this signaling pathway was further underscored by the observation that endothelial cell migration, which requires a transient reduction of cell-cell adhesion, was blocked when signaling through ROS was inhibited. In conclusion, Rac-mediated production of ROS represents a previously unrecognized means of regulating VE-cadherin function and may play an important role in the (patho)physiology associated with inflammation and endothelial damage as well as with endothelial cell migration and angiogenesis.


Subject(s)
Actin Cytoskeleton/metabolism , Cadherins/metabolism , Cell Adhesion/physiology , Endothelium, Vascular/metabolism , Reactive Oxygen Species/metabolism , rac GTP-Binding Proteins/metabolism , Antigens, CD , Cells, Cultured , Endothelium, Vascular/cytology , Fibroblasts/cytology , Fibroblasts/metabolism , Humans , Phosphorylation , Signal Transduction/physiology , Transduction, Genetic , Tyrosine/metabolism , rho GTP-Binding Proteins/metabolism
15.
J Immunol ; 168(2): 588-96, 2002 Jan 15.
Article in English | MEDLINE | ID: mdl-11777950

ABSTRACT

The success of stem cell transplantation depends on the ability of i.v. infused stem cells to engraft the bone marrow, a process referred to as homing. Efficient homing requires migration of CD34(+) cells across the bone marrow endothelium, most likely through the intercellular junctions. In this study, we show that loss of vascular endothelial (VE)-cadherin-mediated endothelial cell-cell adhesion increases the permeability of monolayers of human bone marrow endothelial cells (HBMECs) and stimulates the transendothelial migration of CD34(+) cells in response to stromal cell-derived factor-1alpha. Stromal cell-derived factor-1alpha-induced migration was dependent on VCAM-1 and ICAM-1, even in the absence of VE-cadherin function. Cross-linking of ICAM-1 to mimic the leukocyte-endothelium interaction induced actin stress fiber formation but did not induce loss of endothelial integrity, whereas cross-linking of VCAM-1 increased the HBMEC permeability and induced gaps in the monolayer. In addition, VCAM-1-mediated gap formation in HBMEC was accompanied by and dependent on the production of reactive oxygen species. These data suggest that modulation of VE-cadherin function directly affects the efficiency of transendothelial migration of CD34(+) cells and that activation of ICAM-1 and, in particular, VCAM-1 plays an important role in this process through reorganization of the endothelial actin cytoskeleton and by modulating the integrity of the bone marrow endothelium through the production of reactive oxygen species.


Subject(s)
Bone Marrow/physiology , Cadherins/physiology , Cell Movement/physiology , Endothelium, Vascular/physiology , Hematopoietic Stem Cells/physiology , Antigens, CD , Antigens, CD34/biosynthesis , Cell Adhesion/physiology , Cell Line, Transformed , Endothelium/cytology , Endothelium/physiology , Endothelium, Vascular/cytology , Gap Junctions/metabolism , Gap Junctions/physiology , Hematopoietic Stem Cells/cytology , Humans , Intercellular Adhesion Molecule-1/physiology , Permeability , Reactive Oxygen Species/pharmacology , Vascular Cell Adhesion Molecule-1/physiology , rho GTP-Binding Proteins/physiology
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