ABSTRACT
Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue. This technique is particularly informative for studying how stem cells build and maintain the mammary epithelium during development and pregnancy. Here we describe two experimental systems based on Cre/loxP technology (CreERT2/loxP and rtTA/tetO-Cre/loxP), which allow the inducible, permanent labeling of mammary epithelial cells following the administration of either tamoxifen or doxycycline.
Subject(s)
Cell Lineage/physiology , Mammary Glands, Animal/physiology , Stem Cells/physiology , Animals , Breast/physiology , Cell Lineage/drug effects , Doxycycline/pharmacology , Epithelial Cells/drug effects , Epithelial Cells/physiology , Female , Mammary Glands, Animal/drug effects , Mice , Stem Cells/drug effects , Tamoxifen/pharmacologyABSTRACT
The ENBDC workshop "Methods in Mammary Gland Development and Cancer" is an established international forum to showcase the latest technical advances in the field. The eighth meeting focused on emerging concepts and technologies for studying normal and neoplastic breast development.
