Suspicion of abdominal injuries in high-energy trauma patients: which clinical factors influence decision making for diagnostic imaging?

Background: The choice of diagnostic imaging for high-energy trauma (HET) patients is highly debated. Currently, different diagnostic imaging is used in trauma centres to identify abdominal injuries. However, it remains unclear when physicians have a suspicion for abdominal injuries, and when diagnostic imaging is performed. Over-triage may lead to unnecessary diagnostics in relatively minor injured HET-patients.Purpose: We investigated which clinical factors influence the decision to perform a focused assessment with sonography in trauma (FAST) or abdominal computed tomography (CT) in HET-patients. Additionally, we investigated which clinical factors determined whether HET-patients were admitted to the hospital or discharged from the emergency department.Methods: We performed a retrospective data analysis of all HET-patients in a single level II trauma centre in the Netherlands, between June 2015 and January 2017.Results: 316 HET-patients were included in this study. We found two clinical factors that proved to significantly predict whether a FAST or abdominal CT was performed: abdominal pain and the degree of concomitant injury. Furthermore, we found that the degree of concomitant injury as well as low haemoglobin levels proved to significantly predict whether a patient was admitted to the hospital for observation.Conclusion: This study clarifies on which clinical factors the decision is taken to perform diagnostic imaging to identify abdominal injuries. Future prospective multicentre studies should clarify whether these clinical factors are trustworthy predictors of abdominal injuries, and whether patients can safely be discharged after trauma work-up.

[Lactic acidosis in the postictal state]. / Lactaatacidose in de postictale fase.

Epilepsy is a neurological disorder with an annual incidence in the Netherlands of 30 per 100,000 people. We present two cases of a patient admitted to the emergency department upon experiencing a generalized seizure. In each case, severe metabolic lactic acidosis was identified through routine laboratory diagnostics. Based on their clinical presentation, we had no reasons to suspect another cause of this severe acidosis apart from the seizure. We repeated arterial blood sample one to two hours later and found that both pH and lactate were normalized. Severe lactic acidosis may occur in patients who experience seizures but otherwise do not require treatment. Taking an arterial blood sample from these patients in the emergency setting will be of limited value, because in most patients hyperlactatemia in the postictal state is self-limiting. In some patients, however, a persistent hyperlactatemia may indicate a serious underlying pathology. It is therefore advisable to repeat an arterial blood sample a few hours later.

Use of the HPV MLPA assay in cervical cytology for the prediction of high grade lesions.

Current screening methods for uterine cervical cancer such as Papanicolaou smears and/or high risk human Papillomavirus (HR-HPV) detection have a high negative predictive value but a low positive predictive value for the presence of high grade cervical lesions. Therefore, new parameters are needed to reduce the rate of unnecessary referrals for colposcopy. The predictive value of the HPV multiplex ligation-dependent probe amplification (MLPA) assay, which can assess simultaneously HPV16/18 viral load and viral integration, was evaluated. The assay was applied to 170 cervical cytological samples, and the results were correlated with the matching histological follow-up. The GP5+/6+ assay and qPCR were used as a control for HR-HPV typing. The MLPA assay classified a higher percentage of cases as high-risk (high-viral load and/or viral integration) with higher grades of dysplasia. There was a high correlation between the HPV MLPA assay and qPCR for viral load and HPV genotyping, and between the MLPA assay and the GP5+/6+ assay for HPV genotyping. The sensitivity and specificity of the HPV MLPA assay for the detection of high-grade lesions were 44% and 93%, respectively. This study demonstrates that the HPV MLPA assay can reliably detect HPV 16/18, viral load, and viral integration in cytological samples. Also, high-risk classification correlated well with the presence of high-grade dysplasia. However, for the implementation of the MLPA assay into clinical practice, additional HR-HPV types need to be included to increase the sensitivity of the assay, and thereby increase its negative predictive value.