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1.
J Behav Ther Exp Psychiatry ; 82: 101896, 2024 03.
Article in English | MEDLINE | ID: mdl-37741178

ABSTRACT

BACKGROUND AND OBJECTIVES: In recent years eye-tracking studies have provided converging evidence that socially anxious individuals avoid looking at other people's faces in social situations. In addition to these objective measures, the Gaze Anxiety Rating Scale (GARS) has increasingly been used as a self-report measure of gaze avoidance. However, extant results concerning its predictive validity were inconsistent. Moreover, no study has considered social anxiety and gaze anxiety together to examine their relative contributions to actual gaze behavior. METHODS: To address these two questions, eye-tracking data collected from 81 female students during the initial 6 min of a face-to-face conversation with a female confederate were analyzed. Gaze anxiety and social anxiety were measured via the GARS and the Leibowitz Social Anxiety Scale. RESULTS: The results revealed that gaze anxiety was associated with reduced face gaze while speaking. Social anxiety was not only associated with decreased face gaze during speaking, but also across the initial conversation. Moreover, there was no evidence that gaze anxiety made an additional contribution to social anxiety in predicting face gaze behavior. LIMITATIONS: This study examined face gaze instead of eye gaze. Additionally, the self-report data were not collected on the same day as the eye-tracking data. CONCLUSIONS: The findings indicate that, in a community sample, gaze anxiety does predict actual gaze behavior during a face-to-face initial encounter, but social anxiety is a stronger predictor.


Subject(s)
Anxiety Disorders , Anxiety , Humans , Female , Fear , Fixation, Ocular , Communication
2.
Anxiety Stress Coping ; 36(4): 460-474, 2023 07.
Article in English | MEDLINE | ID: mdl-36153759

ABSTRACT

BACKGROUND: Social anxiety has long been related to reduced eye contact, and this feature is seen as a causal and a maintaining factor of social anxiety disorder. The present research adds to the literature by investigating the relationship between social anxiety and visual avoidance of faces in a reciprocal face-to-face conversation, while taking into account two aspects of conversations as potential moderating factors: conversational role and level of intimacy. METHOD: Eighty-five female students (17-25 years) completed the Leibowitz Social Anxiety Scale and had a face-to-face getting-acquainted conversation with a female confederate. We alternated conversational role (talking versus listening) and manipulated intimacy of the topics (low versus high). Participants' gaze behavior was registered with Tobii eye-tracking glasses. Three dependent measures were extracted regarding fixations on the face of the confederate: total duration, proportion of fixations, and mean duration. RESULTS: The results revealed that higher levels of social anxiety were associated with reduced face gaze on all three measures. The relation with total fixation duration was stronger for low intimate topics. The relation with mean fixation duration was stronger during listening than during speaking. CONCLUSION: The results highlight the importance of studying gaze behavior in a naturalistic social interaction.


Subject(s)
Phobia, Social , Social Interaction , Humans , Female , Eye Movements , Fear , Anxiety
3.
J Biol Chem ; 295(22): 7726-7742, 2020 05 29.
Article in English | MEDLINE | ID: mdl-32332099

ABSTRACT

G protein-coupled receptor signaling is required for the navigation of immune cells along chemoattractant gradients. However, chemoattractant receptors may couple to more than one type of heterotrimeric G protein, each of which consists of a Gα, Gß, and Gγ subunit, making it difficult to delineate the critical signaling pathways. Here, we used knockout mouse models and time-lapse microscopy to elucidate Gα and Gß subunits contributing to complement C5a receptor-mediated chemotaxis. Complement C5a-mediated chemokinesis and chemotaxis were almost completely abolished in macrophages lacking Gnai2 (encoding Gαi2), consistent with a reduced leukocyte recruitment previously observed in Gnai2-/- mice, whereas cells lacking Gnai3 (Gαi3) exhibited only a slight decrease in cell velocity. Surprisingly, C5a-induced Ca2+ transients and lamellipodial membrane spreading were persistent in Gnai2-/- macrophages. Macrophages lacking both Gnaq (Gαq) and Gna11 (Gα11) or both Gna12 (Gα12) and Gna13 (Gα13) had essentially normal chemotaxis, Ca2+ signaling, and cell spreading, except Gna12/Gna13-deficient macrophages had increased cell velocity and elongated trailing ends. Moreover, Gnaq/Gna11-deficient cells did not respond to purinergic receptor P2Y2 stimulation. Genetic deletion of Gna15 (Gα15) virtually abolished C5a-induced Ca2+ transients, but chemotaxis and cell spreading were preserved. Homozygous Gnb1 (Gß1) deletion was lethal, but mice lacking Gnb2 (Gß2) were viable. Gnb2-/- macrophages exhibited robust Ca2+ transients and cell spreading, albeit decreased cell velocity and impaired chemotaxis. In summary, complement C5a-mediated chemotaxis requires Gαi2 and Gß2, but not Ca2+ signaling, and membrane protrusive activity is promoted by G proteins that deplete phosphatidylinositol 4,5-bisphosphate.


Subject(s)
Calcium Signaling , Chemotaxis , Heterotrimeric GTP-Binding Proteins/metabolism , Macrophages/metabolism , Models, Biological , Receptor, Anaphylatoxin C5a/metabolism , Animals , Heterotrimeric GTP-Binding Proteins/genetics , Mice, Knockout , Receptor, Anaphylatoxin C5a/genetics
4.
J Vis Exp ; (158)2020 04 02.
Article in English | MEDLINE | ID: mdl-32310228

ABSTRACT

Chemotaxis is receptor-mediated guidance of cells along a chemical gradient, whereas chemokinesis is the stimulation of random cell motility by a chemical. Chemokinesis and chemotaxis are fundamental for the mobilization and deployment of immune cells. For example, chemokines (chemotactic cytokines) can rapidly recruit circulating neutrophils and monocytes to extravascular sites of inflammation. Chemoattractant receptors belong to the large family of G protein-coupled receptors. How chemoattractant (i.e., ligand) gradients direct cell migration via G protein-coupled receptor signaling is not yet fully understood. In the field of immunology, neutrophils are popular model cells for studying chemotaxis in vitro. Here we describe a real-time two-dimensional (2D) chemotaxis assay tailored for mouse resident macrophages, which have traditionally been more difficult to study. Macrophages move at a slow pace of ~1 µm/min on a 2D surface and are less well suited for point-source migration assays (e.g., migration towards the tip of a micropipette filled with chemoattractant) than neutrophils or Dictyostelium discoideum, which move an order of magnitude faster. Widely used Transwell assays are useful for studying the chemotactic activity of different substances, but do not provide information on cell morphology, velocity, or chemotactic navigation. Here we describe a time-lapse microscopy-based macrophage chemotaxis assay that allows quantification of cell velocity and chemotactic efficiency and provides a platform to delineate the transducers, signal pathways, and effectors of chemotaxis.


Subject(s)
Chemotaxis , Macrophages/cytology , Time-Lapse Imaging/methods , Animals , Dictyostelium/cytology , Macrophages/metabolism , Mice , Monocytes/cytology , Neutrophils/cytology , Receptors, G-Protein-Coupled/metabolism , Signal Transduction
5.
J Anxiety Disord ; 70: 102193, 2020 03.
Article in English | MEDLINE | ID: mdl-32058889

ABSTRACT

Although visual avoidance of faces is a hallmark feature of social anxiety disorder (SAD) on clinical and theoretical grounds, empirical support is equivocal. This review aims to clarify under which conditions socially anxious individuals display visual avoidance of faces. Through a systematic search in Web of Science and PubMed up to March 2019 we identified 61 publications that met the inclusion criteria. We discuss the influence of three factors on the extent to which socially anxious individuals avoid looking at faces: (a) severity of social anxiety symptoms (diagnosed SAD versus High Social Anxiety levels in community samples [HSA] or related characteristics [Shyness, Fear of Negative Evaluation]), (b) three types of social situation (computer facial-viewing tasks, speaking tasks, social interactions), and (c) development (age-group). Adults with SAD exhibit visual avoidance across all three types of social situations, whereas adults with HSA exhibit visual avoidance in speaking and interaction tasks but not in facial-viewing tasks. The relatively few studies with children and adolescents suggest that visual avoidance emerges during adolescence. The findings are discussed in the context of cognitive-behavioral and skills-deficit models. Suggestions for future research include the need for developmental studies and more fine-grained analyses of specific areas of the face.


Subject(s)
Anxiety/physiopathology , Anxiety/psychology , Avoidance Learning , Face , Phobia, Social/physiopathology , Phobia, Social/psychology , Vision, Ocular , Fear , Humans
6.
Mem Cognit ; 47(8): 1619-1620, 2019 Nov.
Article in English | MEDLINE | ID: mdl-31215013

ABSTRACT

In the article "Effects of Grammar Complexity on Artificial Grammar Learning" by E. Van den Bos and F. Poletiek, published in Memory & Cognition, 2008, 36(6), 1122-1131, doi:10.3758/MC.36.6.1122, an error was made in the computation of topological entropy (TE).

7.
Behav Cogn Psychother ; 47(2): 148-163, 2019 Mar.
Article in English | MEDLINE | ID: mdl-29769157

ABSTRACT

BACKGROUND: Cognitive behavioural therapy (CBT) has relatively poor outcomes for youth with social anxiety, possibly because broad-based CBT is not tailored to their specific needs. Treatment of social anxiety in youth may need to pay more attention to negative social cognitions that are considered a key factor in social anxiety development and maintenance. AIMS: The aim of the present study was to learn more about the role of performance quality in adolescents' cognitions about their social performance and, in particular, the moderating role social anxiety plays in the relationship between performance quality and self-cognitions. METHOD: A community sample of 229 participants, aged 11 to 18 years, gave a speech and filled in questionnaires addressing social anxiety, depression, expected and self-evaluated performance, and post-event rumination. Independent observers rated the quality of the speech. The data were analysed using moderated mediation analysis. RESULTS: Performance quality mediated the link between expected and self-evaluated performance in adolescents with low and medium levels of social anxiety. For adolescents with high levels of social anxiety, only a direct link between expected and self-evaluated performance was found. Their self-evaluation was not related to the quality of their performance. Performance quality also mediated the link between expected performance and rumination, but social anxiety did not moderate this mediation effect. CONCLUSIONS: Results suggest that a good performance does not help socially anxious adolescents to replace their negative self-evaluations with more realistic ones. Specific cognitive intervention strategies should be tailored to the needs of socially anxious adolescents who perform well.


Subject(s)
Anxiety/diagnosis , Anxiety/psychology , Diagnostic Self Evaluation , Rumination, Cognitive , Self-Assessment , Speech , Adolescent , Anxiety/therapy , Child , Depression/diagnosis , Depression/psychology , Female , Humans , Male , Self Report , Surveys and Questionnaires
8.
Psychoneuroendocrinology ; 78: 159-167, 2017 04.
Article in English | MEDLINE | ID: mdl-28209542

ABSTRACT

Contradictory findings have been reported on the relation between social anxiety and the cortisol response to social evaluation in youth. The present longitudinal study aimed to clarify this relation by taking pubertal development into account. Data were collected in two waves, two years apart, for a community sample of 196 participants, aged 8-17 years at Time 1. Pubertal development and social anxiety were assessed with self-report questionnaires. Salivary cortisol was obtained before and after participants completed the Leiden Public Speaking Task. Data were analyzed using regression analysis with clustered bootstrap. The dependent variable was the cortisol area under the curve. Social anxiety and pubertal development scores were decomposed into between- and within-participants components. Between participants, the relation between social anxiety and the cortisol response to public speaking varied with pubertal development: socially anxious individuals showed higher responses at low levels of pubertal development, but lower responses at high levels of pubertal development. Within participants, an increase in social anxiety over time was associated with a lower cortisol response. The results are in line with the suggestion that the responses of socially anxious individuals change from elevated in childhood to attenuated in adolescence and adulthood. Attenuation of the cortisol response is explained by theories proposing that the stress response changes with the duration of the stressor.


Subject(s)
Anxiety/physiopathology , Hydrocortisone/analysis , Hypothalamo-Hypophyseal System/physiopathology , Pituitary-Adrenal System/physiopathology , Adolescent , Child , Female , Humans , Male , Saliva/chemistry , Self Report , Speech , Surveys and Questionnaires
9.
Dev Psychol ; 52(7): 1151-63, 2016 07.
Article in English | MEDLINE | ID: mdl-27177160

ABSTRACT

Adolescents become increasingly sensitive to social evaluation. Some previous studies have related this change to pubertal development. The present longitudinal study examined the role of sociocognitive development. We investigated whether or not the transition to recursive thinking, the ability to think about (others') thoughts, would be associated with changes in the magnitude and timing of the cortisol response to social evaluation. Salivary cortisol was obtained during the Leiden Public Speaking Task. The task was administered twice with a 2-year interval to 221 participants, aged 9-17 years at Time 1. The area under the curve was computed to assess the magnitude of the overall cortisol response. Two difference scores, reflecting speech anticipation and speech delivery, were computed to assess the timing of the cortisol response. Recursive thinking was measured with a cartoon description task. Regression analyses with clustered bootstrap controlling for pubertal development, age, and general cognitive functioning showed that the transition to recursive thinking predicted an increase in the cortisol response to speech anticipation, but was unrelated to the magnitude of the overall cortisol response. This is in line with the view that increasing sensitivity to social evaluation in adolescence is mainly due to the effects of pubertal hormones on affective regions of the brain. Sociocognitive development affected the timing rather than the magnitude of the cortisol response. The results suggest that recursive thinking enables earlier realization of social-evaluative threat. (PsycINFO Database Record


Subject(s)
Adolescent Development/physiology , Hydrocortisone/metabolism , Social Behavior , Stress, Psychological/metabolism , Thinking , Adolescent , Anxiety/etiology , Anxiety/metabolism , Area Under Curve , Child , Cognition , Cohort Studies , Female , Humans , Longitudinal Studies , Male , Psychological Tests , Puberty/metabolism , Puberty/psychology , Regression Analysis , Saliva/metabolism , Speech/physiology
10.
Front Psychol ; 6: 158, 2015.
Article in English | MEDLINE | ID: mdl-25745408

ABSTRACT

This study investigated whether the negative effect of complexity on artificial grammar learning could be compensated by adding semantics. Participants were exposed to exemplars from a simple or a complex finite state grammar presented with or without a semantic reference field. As expected, performance on a grammaticality judgment test was higher for the simple grammar than for the complex grammar. For the simple grammar, the results also showed that participants presented with a reference field and instructed to decode the meaning of each exemplar (decoding condition) did better than participants who memorized the exemplars without semantic referents (memorize condition). Contrary to expectations, however, there was no significant difference between the decoding condition and the memorize condition for the complex grammar. These findings indicated that the negative effect of complexity remained, despite the addition of semantics. To clarify how the presence of a reference field influenced the learning process, its effects on the acquisition of two types of knowledge (first- and second-order dependencies) and on participants' awareness of their knowledge were examined. The results tentatively suggested that the reference field enhanced the learning of second-order dependencies. In addition, participants in the decoding condition realized when they had knowledge relevant to making a grammaticality judgment, whereas participants in the memorize condition demonstrated some knowledge of which they were unaware. These results are in line with the view that the reference field enhanced structure learning by making certain dependencies more salient. Moreover, our findings stress the influence of complexity on artificial grammar learning.

11.
Psychophysiology ; 52(3): 316-24, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25267560

ABSTRACT

Long-term stability of individual differences in stress responses has repeatedly been demonstrated in adults, but few studies have investigated the development of stability in adolescence. The present study was the first to investigate the stability of individual differences in heart rate, parasympathetic (RMSSD, pNN50, HF), sympathetic (LF/HF, SC), and HPA-axis (salivary cortisol) responses in a youth sample (8-19 years). Responses to public speaking were measured twice over 2 years. Stability was moderate for absolute responses and task delta responses of HR, RMSSD, pNN50, and HF. Stability was lower for SC and task delta responses of LF/HF and cortisol. Anticipation delta responses showed low stability for HR and cortisol. The latter was moderated by age or puberty, so that individual differences were more stable in more mature individuals. The results support the suggestion that stress responses may be reset during adolescence, but only for the HPA axis.


Subject(s)
Autonomic Nervous System/physiopathology , Hypothalamo-Hypophyseal System/physiopathology , Individuality , Pituitary-Adrenal System/physiopathology , Speech/physiology , Stress, Psychological/physiopathology , Adolescent , Child , Female , Heart Rate/physiology , Humans , Hydrocortisone/analysis , Male , Saliva/chemistry , Young Adult
12.
Child Dev ; 85(1): 220-36, 2014.
Article in English | MEDLINE | ID: mdl-23638912

ABSTRACT

Stress responses to social evaluation are thought to increase during adolescence, which may be due to pubertal maturation. However, empirical evidence is scarce. This study is the first to investigate the relation between pubertal development and biological responses to a social-evaluative stressor longitudinally. Participants performed the Leiden Public Speaking Task twice, with a 2-year interval (N = 217; age at Time 1: 8-17 years). The results support an increase in sensitivity to social evaluation during adolescence. The overall cortisol and alpha-amylase responses increased-both between and within participants-and were more strongly related to self-reported pubertal development than to age. The cortisol response shifted from speech delivery toward anticipation. The alpha-amylase response increased in both phases.


Subject(s)
Hydrocortisone/metabolism , Puberty/physiology , Salivary alpha-Amylases/metabolism , Social Behavior , Stress, Psychological/metabolism , Adolescent , Adolescent Development/physiology , Child , Female , Humans , Longitudinal Studies , Male , Stress, Psychological/enzymology
13.
Psychol Res ; 74(2): 138-51, 2010 Mar.
Article in English | MEDLINE | ID: mdl-19212781

ABSTRACT

In the contextual cueing paradigm, Endo and Takeda (in Percept Psychophys 66:293-302, 2004) provided evidence that implicit learning involves selection of the aspect of a structure that is most useful to one's task. The present study attempted to replicate this finding in artificial grammar learning to investigate whether or not implicit learning commonly involves such a selection. Participants in Experiment 1 were presented with an induction task that could be facilitated by several characteristics of the exemplars. For some participants, those characteristics included a perfectly predictive feature. The results suggested that the aspect of the structure that was most useful to the induction task was selected and learned implicitly. Experiment 2 provided evidence that, although salience affected participants' awareness of the perfectly predictive feature, selection for implicit learning was mainly based on usefulness.


Subject(s)
Knowledge , Learning/physiology , Linguistics , Retention, Psychology/physiology , Adolescent , Adult , Analysis of Variance , Attention/physiology , Cues , Female , Humans , Language Tests , Male , Mental Recall/physiology , Photic Stimulation , Reaction Time/physiology
14.
Mem Cognit ; 36(6): 1122-31, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18927030

ABSTRACT

The present study identified two aspects of complexity that have been manipulated in the implicit learning literature and investigated how they affect implicit and explicit learning of artificial grammars. Ten finite state grammars were used to vary complexity. The results indicated that dependency length is more relevant to the complexity of a structure than is the number of associations that have to be learned. Although implicit learning led to better performance on a grammaticality judgment test than did explicit learning, it was negatively affected by increasing complexity: Performance decreased as there was an increase in the number of previous letters that had to be taken into account to determine whether or not the next letter was a grammatical continuation. In particular, the results suggested that implicit learning of higher order dependencies is hampered by the presence of longer dependencies. Knowledge of first-order dependencies was acquired regardless of complexity and learning mode.


Subject(s)
Attention , Linguistics , Semantics , Verbal Learning , Adolescent , Adult , Association Learning , Female , Generalization, Psychological , Humans , Male , Mental Recall , Transfer, Psychology
15.
Cognition ; 85(2): 177-87, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12127698

ABSTRACT

Recognizing oneself, easy as it appears to be, seems at least to require awareness of one's body and one's actions. To investigate the contribution of these factors to self-recognition, we presented normal subjects with an image of both their own and the experimenter's hand. The hands could make the same, a different or no movement and could be displayed in various orientations. Subjects had to tell whether the indicated hand was theirs or not. The results showed that a congruence between visual signals and signals indicating the position of the body is one component on which self-recognition is based. Recognition of one's actions is another component. Subjects had most difficulty in recognizing their hand when movements were absent. When the two hands made different movements, subjects relied exclusively on the movement cue and recognition was almost perfect. Our findings are in line with pathological alterations in the sense of body and the sense of action.


Subject(s)
Awareness , Body Image , Kinesthesis , Recognition, Psychology , Adult , Female , Humans , Male
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