Risk assessment of fungal materials.

Sustainable fungal materials have a high potential to replace non-sustainable materials such as those used for packaging or as an alternative for leather and textile. The properties of fungal materials depend on the type of fungus and substrate, the growth conditions and post-treatment of the material. So far, fungal materials are mainly made with species from the phylum Basidiomycota, selected for the mechanical and physical properties they provide. However, for mycelium materials to be implemented in society on a large scale, selection of fungal species should also be based on a risk assessment of the potential to be pathogenic, form mycotoxins, attract insects, or become an invasive species. Moreover, production processes should be standardized to ensure reproducibility and safety of the product.

Flexible Fungal Materials: Shaping the Future.

Fungi are a revolutionary, smart, and sustainable manufacturing platform that can be used to upcycle byproducts and wastes into flexible fungal materials (FFMs) such as chitin- and ß-glucan-based foams, paper, and textiles. With highly adaptable manufacturing pathways, the efficiency and properties of these materials depend on the biomass source and fermentation method. Liquid substrates provide fast, upscalable, and compact production processes but are susceptible to contamination and are limited to paper-like materials for printing, wound dressings, and membranes. Solid-state fermentation is cheaper but struggles to deliver homogeneous fungal growth and is used to produce fungal foams for packaging, insulation, textiles, and leather substitutes. The broad range of applications and uses of biological organisms in materials hallmarks fungi as forerunners in improving environmental sustainability globally.

Fungal mycelium classified in different material families based on glycerol treatment.

Fungal mycelium is an emerging bio-based material. Here, mycelium films are produced from liquid shaken cultures that have a Young's modulus of 0.47 GPa, an ultimate tensile strength of 5.0 MPa and a strain at failure of 1.5%. Treating the mycelial films with 0-32% glycerol impacts the material properties. The largest effect is observed after treatment with 32% glycerol decreasing the Young's modulus and the ultimate tensile strength to 0.003 GPa and 1.8 MPa, respectively, whereas strain at failure increases to 29.6%. Moreover, glycerol treatment makes the surface of mycelium films hydrophilic and the hyphal matrix absorbing less water. Results show that mycelium films treated with 8% and 16-32% glycerol classify as polymer- and elastomer-like materials, respectively, while non-treated films and films treated with 1-4% glycerol classify as natural material. Thus, mycelium materials can cover a diversity of material families.