Mood variability during adolescent development and its relation to sleep and brain development.

Mood swings, or mood variability, are associated with negative mental health outcomes. Since adolescence is a time when mood disorder onset peaks, mood variability during this time is of significant interest. Understanding biological factors that might be associated with mood variability, such as sleep and structural brain development, could elucidate the mechanisms underlying mood and anxiety disorders. Data from the longitudinal Leiden self-concept study (N = 191) over 5 yearly timepoints was used to study the association between sleep, brain structure, and mood variability in healthy adolescents aged 11-21 at baseline in this pre-registered study. Sleep was measured both objectively, using actigraphy, as well as subjectively, using a daily diary self-report. Negative mood variability was defined as day-to-day negative mood swings over a period of 5 days after an MRI scan. It was found that negative mood variability peaked in mid-adolescence in females while it linearly increased in males, and average negative mood showed a similar pattern. Sleep duration (subjective and objective) generally decreased throughout adolescence, with a larger decrease in males. Mood variability was not associated with sleep, but average negative mood was associated with lower self-reported energy. In addition, higher thickness in the dorsolateral prefrontal cortex (dlPFC) compared to same-age peers, suggesting a delayed thinning process, was associated with higher negative mood variability in early and mid-adolescence. Together, this study provides an insight into the development of mood variability and its association with brain structure.

The role of autism and alexithymia traits in behavioral and neural indicators of self-concept and self-esteem in adolescence.

LAY ABSTRACT: Developing a positive view of the self is important for maintaining a good mental health, as feeling negative about the self increases the risk of developing internalizing symptoms such as feelings of depression and anxiety. Even though autistic individuals regularly struggle with these internalizing feelings, and both self-concept and internalizing feelings are known to develop during adolescence, there is a lack of studies investigating the development of positive self-concept and self-esteem in autistic adolescents. Here, we studied academic, physical, and prosocial self-concept as well as self-esteem in adolescent males with and without autism on both the behavioral and neural level. We additionally focused on similarities in one's own and peers' perspectives on the self, and we assessed a potential role of alexithymia (i.e. having trouble identifying and describing one's feelings) in developing a more negative view of the self. Results showed that there were no group differences in self-esteem, self-concept, or underlying neural activation. This shows that autistic adolescent males use the same neural processes when they evaluate their traits. However, regardless of clinical diagnosis, a higher number of autism traits was related to a less positive physical and prosocial self-concept, whereas more difficulty identifying one's feelings was related to lowered self-esteem and less activation in medial prefrontal cortex during self-evaluations. Therefore, in treatment of autistic adolescents with low self-esteem, it is important to take into account and possibly aim to improve alexithymic traits as well.

Longitudinal self-concept development in adolescence.

This longitudinal behavioral neuroimaging study tested two hypotheses concerning self-concept development in adolescence: domain-specific self-concept and similarity between own (direct) and perceived peers' (reflected) opinions of the self. Participants (N = 189; 10-24 years) evaluated their traits in academic, physical appearance and prosocial domains from direct and reflected perspectives in an functional magnetic resonance imaging session across three time points (TP1: n = 160; TP2: n = 151; TP3: n = 144). Behaviorally, we observed a mid-adolescent dip in self-concept positivity, which was strongest for the academic domain, showing domain differentiation in mid-adolescence. Self-evaluations were associated with activity in, e.g. medial prefrontal cortex (mPFC) and temporal-parietal junction (TPJ). mPFC showed an adolescent-emerging peak in activation, pronounced more for direct than reflected self-evaluations. TPJ activation was generally stronger for reflected self-evaluations, and activation linearly increased with age for both reflected and direct self-evaluations. Longitudinal prediction analyses showed that positivity of self-evaluations predicted increases in self-concept clarity and less fear of negative evaluation 1 and 2 years later, highlighting the developmental benefits of acquiring a positive self-concept. Together, we show that adolescent self-development is characterized by dissociable neural patterns underlying self-evaluations in different domains, and from reflected and direct perspectives, confirming adolescence as a formative phase for developing a coherent and positive self-concept.

Variability and change in adolescents' prosocial behavior across multiple time scales.

We examined variability and change in adolescents' prosocial behaviors directed to peers and friends across four time scales: two-years, one-year, two-monthly, and daily. Data from three longitudinal datasets with a total of 569 adolescents (55.7% girl, Mage  = 15.23, SD = 3.90) were included. The overall time-related stability of prosocial behavior across time scales was moderate to excellent. Variability did not differ between early (age 10-15) and late (age 16-21) adolescence, but late adolescence was associated with higher mean levels of prosociality. Finally, results indicated that prosocial behaviors measured over longer periods (i.e., two-years and one-year) were positively associated with cognitive processes (perspective taking), whereas prosocial behaviors measured over shorter periods (i.e., two-monthly) were positively associated with affective processes (empathy).

Longitudinal neural and behavioral trajectories of charity contributions across adolescence.

This study examined the development of prosocial charity donations and neural activity in the ventral striatum when gaining rewards for self and for charity. Participants 10-22 years (95% European heritage) participated in three annual behavioral-fMRI waves (T1: n = 160, T2: n = 167, T3: n = 175). Behaviorally, donations to charity as measured with an economic Dictator Game increased with age. Perspective taking also increased with age. In contrast, self-gain and charity-gain enjoyment decreased with age. Ventral striatum activity was higher for rewards for self than for charity, but this difference decreased during adolescence. Latent growth curve models revealed that higher donations were associated with a smaller difference between ventral striatum activation for self and charity. These findings show longitudinal brain-donations associations in adolescence.

A perspective on enhancing representative samples in developmental human neuroscience: Connecting science to society.

Marginalized groups are often underrepresented in human developmental neuroscientific studies. This is problematic for the generalizability of findings about brain-behavior mechanisms, as well as for the validity, reliability, and reproducibility of results. In the present paper we discuss selection bias in cohort studies, which is known to contribute to the underrepresentation of marginalized groups. First, we address the issue of exclusion bias, as marginalized groups are sometimes excluded from studies because they do not fit the inclusion criteria. Second, we highlight examples of sampling bias. Recruitment strategies are not always designed to reach and attract a diverse group of youth. Third, we explain how diversity can be lost due to attrition of marginalized groups in longitudinal cohort studies. We provide experience- and evidence-based recommendations to stimulate neuroscientists to enhance study population representativeness via science communication and citizen science with youth. By connecting science to society, researchers have the opportunity to establish sustainable and equal researcher-community relationships, which can positively contribute to tackling selection biases.

Positive and Negative Risk-Taking in Adolescence and Early Adulthood: A Citizen Science Study During the COVID-19 Pandemic.

Sensation seeking is an important underlying factor of both positive and negative forms of risk-taking during adolescence and early adulthood. However, macro-factors such as the global COVID-19 pandemic may influence sensation seeking opportunities and risk-taking behaviors that are considered negative and positive. Therefore, the primary aim of this study was to examine the associations between sensation seeking and behaviors that are considered positive or negative forms of risk-taking during the Covid-19 pandemic in a sample of adolescents and early adults (N = 660, M age = 22.91, SD = 3.14). Using citizen science methods, negative risk-taking was defined as taking unaccepted risks, such as falsifying vaccination reports or deliberately contracting COVID-19. Positive risk-taking was defined as taking socially accepted risks, such as balancing between the risk to infect elderly people and the need to socialize with peers. Results showed that participants with higher levels of sensation seeking took more positive and negative COVID-19 related risks. An additional finding was that sensation seeking was positively associated with the need to contribute to society. This indicates that during adolescence and early adulthood, sensation seeking may be a driving factor for both positive (i.e., socially accepted) and negative (i.e., socially unaccepted) risk-taking in the context of a high-stake global pandemic, arguing against a one-direction negative relation between sensation seeking and risk-taking.

Longitudinal associations between social media use, mental well-being and structural brain development across adolescence.

Youth of today grow up in a digital social world but the effects on well-being and brain development remain debated. This study tracked longitudinal associations between structural brain development, social media use and mental well-being. The study demonstrated two pathways of heterogeneity in brain development. First, adolescents who used social media more than their peers showed higher baseline cortical thickness in lateral prefrontal cortex (PFC) and medial PFC; and stronger decreases in the lateral PFC and temporal parietal junction. In contrast, adolescents with lower mental well-being showed lower baseline levels of surface area in the medial PFC and posterior superior temporal sulcus relative to their peers. Whereas the associations between structural brain development and well-being remained significant after correction for multiple testing, the results for social media use did not survive FDR correction. These findings demonstrate that although social media use and mental well-being were both associated with differential trajectories of brain development, the associations we report are distinct. These results show a nuanced perspective on the presumed relations between social media use and well-being and provide a starting point to further examine neural mechanisms that could explain which adolescents thrive by social media and which might be harmed.

The nature of the self: Neural analyses and heritability estimates of self-evaluations in middle childhood.

How neural correlates of self-concept are influenced by environmental versus genetic factors is currently not fully understood. We investigated heritability estimates of behavioral and neural correlates of self-concept in middle childhood since this phase is an important time window for taking on new social roles in academic and social contexts. To do so, a validated self-concept fMRI task was applied in a twin sample of 345 participants aged between 7 and 9 years. In the self-concept condition, participants were asked to indicate whether academic and social traits applied to them whereas the control condition required trait categorization. The self-processing activation analyses (n = 234) revealed stronger medial prefrontal cortex (mPFC) activation for self than for control conditions. This effect was more pronounced for social-self than academic self-traits, whereas stronger dorsolateral prefrontal cortex (DLPFC) activation was observed for academic versus social self-evaluations. Behavioral genetic modeling (166 complete twin pairs) revealed that 25-52% of the variation in academic self-evaluations was explained by genetic factors, whereas 16-49% of the variation in social self-evaluations was explained by shared environmental factors. Neural genetic modeling (91 complete twin pairs) for variation in mPFC and anterior prefrontal cortex (PFC) activation for academic self-evaluations confirmed genetic and unique environmental influences, whereas anterior PFC activation for social self-evaluations was additionally influenced by shared environmental influences. This indicates that environmental context possibly has a larger impact on the behavioral and neural correlates of social self-concept at a young age. This is the first study demonstrating in a young twin sample that self-concept depends on both genetic and environmental factors, depending on the specific domain.

Explicit and implicit self-esteem in youth with autism spectrum disorders.

LAY ABSTRACT: Having a stable and good self-esteem is important for maintaining a good mental health. However, having low self-esteem is a risk factor for developing depressive, anxious, or uncooperative/aggressive symptoms. While many individuals with an autism spectrum disorder have these symptoms, there is a lack of studies on self-esteem in this group. We studied self-esteem of youth with autism spectrum disorder and the connection to their co-occurring symptoms. To do this, different self-esteem profiles were investigated, including explicit self-esteem (how someone says their self-esteem is after reflecting on it), implicit self-esteem (how someone's self-esteem is on a task that does not give them time to reflect on it), and the difference between both (high explicit with low implicit self-esteem or low explicit with high implicit self-esteem). Our results show that youth with autism spectrum disorder report lower self-esteem than youth without autism spectrum disorder when they have reflected on it (explicit self-esteem). And parents of children with autism spectrum disorder report that their children have even lower self-esteem. Implicit self-esteem was the same for youth with and without autism spectrum disorder. Furthermore, we found that within youth with autism spectrum disorder, there was a negative relationship between explicit self-esteem and depressive symptoms, and between implicit self-esteem and externalizing behavior. Taken together, youth with autism spectrum disorder are at risk for developing low self-esteem and when they do they have a higher risk of developing co-occurring problems. Therefore we stress that it is important to measure and improve the self-esteem of youth with autism spectrum disorder, so they develop less co-occurring problems and have a higher quality of life.

Longitudinal associations between structural prefrontal cortex and nucleus accumbens development and daily identity formation processes across adolescence.

We tested whether adolescents with daily high identity uncertainty showed differential structural brain development across adolescence and young adulthood. Participants (N = 150, MageT1 15.92 years) were followed across three waves, covering 4 years. Self-reported daily educational identity and structural brain data of lateral prefrontal cortex (lPFC)/anterior cingulate cortex (ACC), medial PFC, and nucleus accumbens (NAcc) was collected across three waves. All hypotheses were pre-registered. Latent class growth analyses confirmed 2 identity subgroups: an identity synthesis class (characterized by strong commitments, and low uncertainty), and an identity moratorium class (high daily identity uncertainty). Latent growth curve models revealed, on average, delayed maturation of the lateral PFC/ACC and medial PFC and stable NAcc. Yet, adolescents in identity moratorium showed lower levels and less decline in NAcc gray matter volume. Lateral PFC/ACC and medial PFC trajectories did not differ between identity subgroups. Exploratory analyses revealed that adolescents with higher baseline levels and delayed maturation of lateral PFC/ACC and medial PFC gray matter volume, surface area, and cortical thickness reported higher baseline levels and stronger increases of in-depth exploration. These results provide insight into how individual differences in brain development relate to fluctuations in educational identity development across adolescence and young adulthood.

Direct and reflected self-concept show increasing similarity across adolescence: A functional neuroimaging study.

In adolescence, the perceived opinions of others are important in the construction of one's self-concept. Previous studies found involvement of medial Prefrontal Cortex (mPFC) and temporal-parietal junction (TPJ) in direct (own perspective) and reflected (perceived perspective of others) self-evaluations, but no studies to date examined differences in these processes across adolescence. In this study, 150 adolescents between 11 and 21 years old evaluated their traits from their own perspective and from the perceived perspective of peers in a fMRI session. Results showed overlapping behavioural and neural measures for direct and reflected self-evaluations, in mPFC, precuneus and right TPJ. The difference in behavioural ratings declined with age, and this pattern was mirrored by activity in the mPFC, showing a diminishing difference in activation for direct > reflected self-evaluations with increasing age. Right TPJ was engaged more strongly for reflected > direct evaluations in adolescents who were less positive about themselves, and those who showed who showed less item-by-item agreement between direct and reflected self-evaluations. Together, the results suggest that the internalization of others' opinions in constructing a self-concept occurs on both the behavioural and neural levels across adolescence, which may aid in developing a stable self-concept.

Neural responses for evaluating self and mother traits in adolescence depend on mother-adolescent relationships.

An important task in adolescence is to achieve autonomy while preserving a positive relationship with parents. Previous fMRI studies showed largely overlapping activation in medial prefrontal cortex (mPFC) for evaluating self and close-other traits but separable activation for self and non-close other. Possibly, more similar mPFC activation reflects closeness or warmth in relationships. We investigated neural indicators of the mother-adolescent relationship in adolescents between 11 and 21 years (N = 143). Mother-adolescent relationship was measured using (i) mothers' and adolescents' trait evaluations about each other, (ii) observations of warmth, negativity and emotional support in mother-adolescent conversation and (iii) similarity in adolescents' neural activation for evaluating self vs mother traits. Results showed relatively more similar mPFC activation in adolescents who evaluated their mothers' traits more positively, suggesting that this is possibly a neural indicator of mother-adolescent relationship quality. Furthermore, mid-adolescence was characterized by more negative mother-adolescent interaction compared to early and late adolescence. This effect co-occurred with mid-adolescent peaks in dorsal striatum, dorsal anterior cingulate cortex and superior temporal sulcus activation in evaluating traits of self vs mother. These results suggest more negative relationships and stronger self-focus in mid-adolescence.

Alexithymic traits can explain the association between puberty and symptoms of depression and anxiety in adolescent females.

Symptoms of internalizing disorders such as depression and anxiety increase in adolescence, especially in females. However, gender differences in depression and anxiety symptoms emerge only after puberty onset. Levels of alexithymia, characterized by difficulties identifying and describing one's emotions, are elevated in depression and anxiety, and fluctuate across adolescence in a gender-specific manner. This study investigated changes in alexithymia across adolescence, and explored the potential role of alexithymia in the development of depression and anxiety, separately for females and males. Accordingly, 140 adolescents aged 11 to 21 years (77 female) completed self-report measures of alexithymia, depression and anxiety, and pubertal development. For females alone, pubertal maturation was associated with alexithymic traits (specifically difficulties identifying and describing feelings), as well as symptoms of depression and anxiety. After accounting for alexithymia, the relationship between puberty and depression and anxiety was absent or reduced in females. Thus, alexithymic traits may have differential consequences for males and females, and possibly contribute towards increased depression and anxiety symptoms in females during adolescence. We propose that developmental changes in alexithymia should be considered when studying the onset and development of internalizing psychological disorders during adolescence.

Task intentions and their implementation into actions: cognitive control from adolescence to middle adulthood.

Cognitive control processes involved in human multitasking arise, mature, and decline across age. This study investigated how age modulates cognitive control at two different levels: the level of task intentions and the level of the implementation of intentions into the corresponding actions. We were particularly interested in specifying maturation of voluntary task choice (intentions) and task-switching execution (their implementations) between adolescence and middle adulthood. Seventy-four participants were assigned to one of the four age groups (adolescents, 12-17 years; emerging adults, 18-22 years; young adults, 23-27 years; middle-aged adults, 28-56 years). Participants chose between two simple cognitive tasks at the beginning of each trial before pressing a spacebar to indicate that the task choice was made. Next, a stimulus was presented in one of the three adjacent boxes, with participants identifying either the location or the shape of the stimulus, depending on their task choice. This voluntary task-switching paradigm allowed us to investigate the intentional component (task choice) separately from its implementation (task execution). Although all participants showed a tendency to repeat tasks more often than switching between them, this repetition bias was significantly stronger in adolescents than in any adult group. Furthermore, participants generally responded slower after task switches than after task repetitions. This switch cost was similar across tasks in the two younger groups but larger for the shape than the location task in the two older groups. Together, our results demonstrate that both task intentions and their implementation into actions differ across age in quite specific ways.