Biohydrogen production by Enterobacter cloacae and Citrobacter freundii in carrier induced granules.

Carrier induced granular particles comprising Enterobacter cloacae and Citrobacter freundii were used to generate H(2) from sucrose in an anaerobic fluidized bed bioreactor. At a hydraulic retention time of 4.5 h, 95.8% of the sucrose was consumed and the rate of H(2) production reached 180 mmol H(2) l h(-1). Biogas composition for H(2) and CO(2) was 42 and 55%, respectively.