Participating in a School-Integrated Daily Exercise Program Improves Motor Performance Significantly in School-Children.

Children's sedentary time has increased, while daily physical activity and motor performance have decreased. We evaluated an integrated school-based exercise program by assessing changes in motor skills after one year and comparing these changes to children who did not participate. We included 303 children from five schools in this longitudinal study and assigned them either to the exercise group (EG; n = 183 with daily exercise program) or the waiting group (WG; n = 120). Motor skills were assessed at baseline and after one year. Mixed modeling was used to analyze inter-group differences of change in motor skills and to determine the effect of sex, age group, and weight status. EG improved more strongly than WG for sprint, side jumps (both p = 0.017), stand and reach (p = 0.012), and ergometry (p ≤ 0.001) when compared to WG. Girls improved more strongly in the sit-ups than boys, second graders more than fifth graders in the backwards balance and the ergometry, and non-overweight children more in the standing long jump than overweight children. The exercise program is effective in increasing motor skills and physical fitness. Girls were not disadvantaged, and overweight children profited as much as their non-overweight peers in all categories but one.

In vitro evaluation of the influence of titanium nitride coating on the retention force between components of two-part abutments.

BACKGROUND: Two-part abutments are typically made up of a base composed of titanium and a ceramic build-up. The long-term outcomes are affected by the mechanical durability. The purpose of the present investigation was to evaluate and compare the retention force of two-part abutment systems with titanium or titanium nitride bases-as fixed with zirconia components and with various surface treatments. METHODS: A total of 60 two-part abutments were investigated-with a titanium base (n = 30) or titanium nitride coated bases (n = 30) and bonded with zirconia ceramic build-ups. The bonding surfaces were treated with aluminium oxide blasting, with an average particle size of 110 µm. The titanium bases were then pretreated with Alloy Primer or Clearfil Ceramic Primer. The ceramic build-ups were only treated with Clearfil Ceramic Primer. For twenty test specimens, no chemical pretreatment was performed. Test specimens were classified into six groups in accordance with the pretreatment (A-F; n = 10). A resin-based luting agent was employed to attach the two parts. Specimens were then subjected to artificial thermal aging (104 cycles with 5 °C/55 °C). The retention force between the two parts was then investigated with a pull-off test. The findings were analyzed by ANOVA statistics. Fracture patterns were examined by electron microscopy. RESULTS: In the absence of primer, titanium nitride coated bases gave significantly greater retention forces than other samples (p < 0.05). Chemical preconditioning with silane coupling agents did not effect on the retention force of coated bases. CONCLUSIONS: The results of the current study suggested that modifying metal surfaces by coating the base with titanium nitride not only has esthetic and biological advantages, but also enhances the mechanical properties of the adhesive bond of two-part abutments.

Biofilm formation on zirconia and titanium over time-An in vivo model study.

OBJECTIVES: The aim of this study was to evaluate volume, vitality and diversity of biofilms on the abutment materials zirconia and titanium as a function of time using an in vivo model for the biofilm formation. MATERIALS AND METHODS: The development of biofilms on zirconia and titanium grade 4 test specimens in the human oral cavity over time was analysed. After pretreatment, a total of 96 titanium and 96 zirconia discs were fixed on 12 composite splints, which were worn by 12 volunteers. After 6 hr, 24 hr, 3 days and 5 days, biofilms on 48 specimens of each material were analysed with confocal laser scanning microscopy (CLSM). The microbiota composition on the other 48 test specimens was examined using full-length 16S sequence analysis. Statistical analysis was performed by SPSS and R, and level of significance was set at 0.05. RESULTS: Confocal laser scanning microscopy analysis of the biofilms revealed significant changes in volume over time on zirconia and titanium. The material did not significantly influence the volume or live/dead ratio at the individual time points. The composition of the microbiome was influenced by the age of the biofilm, but not by the material of the test specimen. The most frequently found bacteria were Streptococcus spp., followed by Neisseria spp., Rothia spp., Haemophilus spp., Gemella spp. and Abiotrophia spp. CONCLUSIONS: On both materials, the quantity and diversity of the microbiome increased over time. Apart from a slight difference in Veillonella abundance at one time point, there were no significant differences between zirconia and titanium.

Fitting accuracy of ceramic veneered Co-Cr crowns produced by different manufacturing processes.

PURPOSE: The purpose of this in vitro study was to evaluate the fitting accuracy of single crowns made from a novel presintered Co-Cr alloy prepared with a computer-aided design and computer-aided manufacturing (CAD/CAM) technique, as compared with crowns manufactured by other digital and the conventional casting technique. Additionally, the influence of oxide layer on the fitting accuracy of specimens was tested. MATERIALS AND METHODS: A total of 40 test specimens made from Co-Cr alloy were investigated according to the fitting accuracy using a replica technique. Four different methods processing different materials were used for the manufacture of the crown copings (milling of presintered (Ceramill Sintron-group_cer_sin) or rigid alloy (Tizian NEM-group_ti_nem), selective laser melting (Ceramill NPL-group_cer_npl), and casting (Girobond NB-group_gir_nb)). The specimens were adapted to a resin model and the outer surfaces were airborne-particle abraded with aluminum oxide. After the veneering process, the fitting accuracy (absolute marginal discrepancy and internal gap) was evaluated by the replica technique in 2 steps, before removing the oxide layer from the intaglio surface of the crowns, and after removing the layer with aluminum oxide airborne-particle abrasion. Statistical analysis was performed by multifactorial analysis of variance (ANOVA) (α=.05). RESULTS: Mean absolute marginal discrepancy ranged between 20 µm (group_cer_npl for specimens of Ceramill NPL) and 43 µm (group_cer_sin for crowns of Ceramill Sintron) with the oxide layer and between 19 µm and 28 µm without the oxide layer. The internal gap varied between 33 µm (group_ti_nem for test samples of Tizian NEM) and 75 µm (group_gir_nb for the base material Girobond NB) with the oxide layer and between 30 µm and 76 µm without the oxide layer. The absolute marginal discrepancy and the internal gap were significantly influenced by the fabrication method used (P<.05). CONCLUSION: Different manufacturing techniques had a significant influence on the fitting accuracy of single crowns made from Co-Cr alloys. However, all tested crowns showed a clinically acceptable absolute marginal discrepancy and internal gap with and without oxide layer and could be recommended under clinical considerations. Especially, the new system Ceramill Sintron showed acceptable values of fitting accuracy so it can be suggested in routine clinical work.

Antibacterial Effect of Cupral® on Oral Biofilms - An In-Vitro Study.

Objective: This study aimed to assess the efficacy of Cupral®, a Ca(OH)2 and Cu2+ based materials used in endodontics, against biofilms of the oral species Streptococcus oralis, Streptococcus gordonii and Aggregatibacter actinomycetemcomitans at different maturation stages. Methods: Biofilms of the bacterial target species were grown in brain heart infusion (BHI) medium for 1 and 5 days on titanium disks (titanium, grade 4) to collect microbial communities at different stages of biofilm maturation. Biofilms were subjected to different Cupral® concentrations (4-, 15- and 50-fold dilution) to assess the antimicrobial- and biofilm dissolving effect. 0.2% chlorhexidine gluconate (CHX) solution was used as a positive control. Biovolume and antibacterial efficacy were analyzed by live/dead staining in combination with confocal laser scanning microscopy (CLSM) to quantify biofilm detachment and antibacterial efficacy. Results: All tested Cupral® concentration showed a strong antibacterial effect on tested bacterial species at all biofilm maturation stages. Efficacy of biofilms detachment was concentration dependent, i.e. higher Cupral® concentrations generally led to increased biofilm detachment. The antibacterial efficacy of tested Cupral® concentration was at least equal to CHX treatment (P=0.03). Conclusion: Cupral® shows a strong anti-biofilm efficacy and may be applied for oral biofilm treatment and control in dental disciplines other than endodontics.

Stabilizing mandibular complete dentures by a single midline implant-influence on quality of life: 2-year results from a randomized clinical trial comparing different loading protocols.

OBJECTIVES: The knowledge about the influence of dental treatment on health-related quality of life (HRQoL) is still limited. The aim of this multicenter randomized controlled clinical trial was to assess the effect of stabilizing an existing complete denture, by means of a single mandibular implant, on HRQoL. Furthermore, the impact of the loading protocol, i.e., immediate or delayed loading, in edentulous patients was evaluated. METHODS: One hundred fifty-eight participants aged 60-89 years were randomly assigned to study group A (immediate loading; n = 81) and to group B (delayed loading; n = 78). All participants received a single midline implant in the mandible. The implants were either immediately loaded (group A) or after a closed healing period of 3 months (group B) by connecting the existing mandibular complete dentures to ball attachments. HRQoL was assessed with the Short Form-36 questionnaire of health (SF-36) at baseline, 4 months, and 24 months after implant loading. RESULTS: Improvement of HRQoL by means of a single implant-retained mandibular overdenture could not be demonstrated after 4 and 24 months of implant loading. Furthermore, the application of two different loading protocols did not influence HRQoL ratings of study participants. CONCLUSION: The loading protocol is not a factor, influencing HRQoL in patients treated by a single midline implant in the edentulous mandible. CLINICAL RELEVANCE: A single midline implant in the edentulous mandible, stabilizing a mandibular complete denture, cannot be recommended for improving HRQoL.

Two-part implant abutments with titanium and ceramic components: Surface modification affects retention forces-An in-vitro study.

OBJECTIVES: Two-part abutments consist of titanium base and ceramic coping. Their long-term success is largely determined by the mechanical stability. The aim of the present study was to investigate the retention forces of two-part implant abutments. The study included zirconia and lithium disilicate ceramics copings, with different surface treatments and resin-based luting agents. MATERIAL AND METHODS: The analysis of retention forces was based on a total of 70 test specimens. Seven surface modifications and three resin-based luting agents were employed for the bonding of components in the seven groups (n = 70). All surfaces of titanium bases-except for a control-were pretreated with aluminum oxide blasting, either alone or in combination with surface activating primers. Surfaces of ceramic copings were also treated mechanically by sandblasting, either alone or with acid etching or different primers. All specimens underwent thermal aging (104 cycles, 5°C/55°C). The retention forces between the two parts were measured with a pull-off test. The results were analyzed by two-way ANOVA statistics. Fracture patterns were evaluated by light and scanning electron microscopy. RESULTS: No mechanical pretreatment of the titanium (group 2) base resulted in the lowest retention. The combination with Monobond plus leads to the highest pull-off forces for both ceramic materials. CONCLUSIONS: Surface modifications and resin-based agents influence the retention of components of two-part abutments. Lithium disilicate ceramic copings reached comparable results of retention to the typically used zirconia copings.

Fracture Strength of Ceramic Posterior Occlusal Veneers for Functional Rehabilitation of an Abrasive Dentition.

PURPOSE: To evaluate the load at fracture and influence of artificial aging of posterior teeth occlusal veneers ("table tops") made of two different ceramics. MATERIALS AND METHODS: A total of 80 table tops were produced from feldspathic ceramic (VM) and zirconia-reinforced lithium silicate ceramic (CD) using computer-aided design/computer-assisted manufacturing (CAD/CAM). Half of the specimens from each ceramic were aged prior to the load test. RESULTS: Mean ± standard deviation (SD) load at fracture was significantly higher for CD (1,571.1 N ± 297.0 N) than for VM (573.6 N ± 86.4 N) (P < .001). After aging, load at fracture increased significantly to 1,819.0 N (± 310.6 N) for CD and to 745.2 N (± 168.3 N) for VM. CONCLUSION: The use of zirconia-reinforced lithium silicate ceramic for molar nonprep table tops should be uncomplicated according to the high load-at-fracture values. Mechanical stability of either ceramic is not compromised by aging.

Retention Forces between Titanium and Zirconia Components of Two-Part Implant Abutments with Different Techniques of Surface Modification.

BACKGROUND: The adhesive connection between titanium base and zirconia coping of two-part abutments may be responsible for the failure rate. A high mechanical stability between both components is essential for the long-term success. PURPOSE: The aim of the present in-vitro study was to evaluate the influence of different surface modification techniques and resin-based luting agents on the retention forces between titanium and zirconia components in two-part implant abutments. MATERIALS AND METHODS: A total of 120 abutments with a titanium base bonded to a zirconia coping were investigated. Two different resin-based luting agents (Panavia F 2.0 and RelyX Unicem) and six different surface modifications were used to fix these components, resulting in 12 test groups (n = 10). The surface of the test specimens was mechanically pretreated with aluminium oxide blasting in combination with application of two surface activating primers (Alloy Primer, Clearfil Ceramic Primer) or a tribological conditioning (Rocatec), respectively. All specimens underwent 10,000 thermal cycles between 5°C and 55°C in a moist environment. A pull-off test was then conducted to determine retention forces between the titanium and zirconia components, and statistical analysis was performed (two-way anova). Finally, fracture surfaces were analyzed by light and scanning electron microscopy. RESULTS: No significant differences were found between Panavia F 2.0 and RelyX Unicem. However, the retention forces were significantly influenced by the surface modification technique used (p < 0.001). For both luting agents, the highest retention forces were found when adhesion surfaces of both the titanium bases and the zirconia copings were pretreated with aluminium oxide blasting, and with the application of Clearfil Ceramic Primer. CONCLUSION: Surface modification techniques crucially influence the retention forces between titanium and zirconia components in two-part implant abutments. All adhesion surfaces should be pretreated by sandblasting. Moreover, a phosphate-based primer serves to enhance long-term retention of the components.

Single dental implant retained mandibular complete dentures--influence of the loading protocol: study protocol for a randomized controlled trial.

BACKGROUND: Over the years, there has been a strong consensus in dentistry that at least two implants are required to retain a complete mandibular denture. It has been shown in several clinical trials that one single median implant can retain a mandibular overdenture sufficiently well for up to 5 years without implant failures, when delayed loading was used. However, other trials have reported conflicting results with in part considerable failure rates when immediate loading was applied. Therefore it is the purpose of the current randomized clinical trial to test the hypothesis that immediate loading of a single mandibular midline implant with an overdenture will result in a comparable clinical outcome as using the standard protocol of delayed loading. METHODS/DESIGN: This prospective nine-center randomized controlled clinical trial is still ongoing. The final patient will complete the trial in 2016. In total, 180 edentulous patients between 60 and 89 years with sufficient complete dentures will receive one median implant in the edentulous mandible, which will retain the existing complete denture using a ball attachment. Loading of the median implant is either immediately after implant placement (experimental group) or delayed by 3 months of submerged healing at second-stage surgery (control group). Follow-up of patients will be performed for 24 months after implant loading. The primary outcome measure is non-inferiority of implant success rate of the experimental group compared to the control group. The secondary outcome measures encompass clinical, technical and subjective variables. The study was funded by the Deutsche Forschungsgemeinschaft (German research foundation, KE 477/8-1). DISCUSSION: This multi-center clinical trial will give information on the ability of a single median implant to retain a complete mandibular denture when immediately loaded. If viable, this treatment option will strongly improve everyday dental practice. TRIAL REGISTRATION: The trial has been registered at Deutsches Register Klinischer Studien (German register of clinical trials) under DRKS-ID: DRKS00003730 since 23 August 2012. (http://www.germanctr.de).

Interfacial adhesion of zirconia/veneer bilayers with different thermal characteristics.

The aim of this study was to investigate how changes in the thermal characteristics of veneer ceramics with almost identical chemical and mechanical properties but with different coefficients of thermal expansion (CTE) can modify their interfacial adhesion to zirconia. 48 bilayers made of one Y-TZP ceramic and four veneer ceramics were fabricated (n=12). Thermal residual stresses were calculated on the basis of the CTE and glass transition temperatures. After defined notching all specimens were loaded in a four-point bending test and the critical loads were recorded which induced stable crack extension at the adhesion interface. The strain energy release rate (G, J/m(2)) was calculated and was taken as a measure of interfacial adhesion. The CTE of the veneer ceramics were significantly correlated with their adhesion to Y-TZP (p<0.001). Interfacial adhesion in zirconia/veneer bilayers is predominantly affected by the thermal characteristics of the veneer ceramic.