ABSTRACT
Electrically active field-effect transistors (FET) based biosensors are of paramount importance in life science applications, as they offer direct, fast, and highly sensitive label-free detection capabilities of several biomolecules of specific interest. In this work, we report a detailed investigation on surface functionalization and covalent immobilization of biomarkers using biocompatible ethanolamine and poly(ethylene glycol) derivate coatings, as compared to the conventional approaches using silica monoliths, in order to substantially increase both the sensitivity and molecular selectivity of nanowire-based FET biosensor platforms. Quantitative fluorescence, atomic and Kelvin probe force microscopy allowed detailed investigation of the homogeneity and density of immobilized biomarkers on different biofunctionalized surfaces. Significantly enhanced binding specificity, biomarker density, and target biomolecule capture efficiency were thus achieved for DNA as well as for proteins from pathogens. This optimized functionalization methodology was applied to InP nanowires that due to their low surface recombination rates were used as new active transducers for biosensors. The developed devices provide ultrahigh label-free detection sensitivities â¼1 fM for specific DNA sequences, measured via the net change in device electrical resistance. Similar levels of ultrasensitive detection of â¼6 fM were achieved for a Chagas Disease protein marker (IBMP8-1). The developed InP nanowire biosensor provides thus a qualified tool for detection of the chronic infection stage of this disease, leading to improved diagnosis and control of spread. These methodological developments are expected to substantially enhance the chemical robustness, diagnostic reliability, detection sensitivity, and biomarker selectivity for current and future biosensing devices.
Subject(s)
Antigens, Protozoan/analysis , Biosensing Techniques/instrumentation , Chagas Disease/diagnosis , Nanowires/chemistry , Trypanosoma cruzi/isolation & purification , Antibodies, Immobilized/chemistry , Antigens, Protozoan/genetics , Biomarkers/analysis , Biosensing Techniques/methods , Chagas Disease/parasitology , DNA/analysis , DNA/genetics , Equipment Design , Humans , Indium/chemistry , Models, Molecular , Phosphines/chemistry , Surface Properties , Transistors, Electronic , Trypanosoma cruzi/geneticsABSTRACT
Optical mode-splitting is an efficient tool to shape and fine-tune the spectral response of resonant nanophotonic devices. The active control of mode-splitting, however, is either small or accompanied by undesired resonance-shifts, often much larger than the resonance splitting. We report a control mechanism that enables reconfigurable and widely tunable mode splitting while efficiently mitigating undesired resonance shifts. This is achieved by actively controlling the excitation of counter-traveling modes in coupled resonators. The transition from a large splitting (80 GHz) to a single-notch resonance is demonstrated using low-power microheaters (35 mW). We show that the spurious resonance shift in our device is only limited by thermal crosstalk, and resonance-shift-free splitting control may be achieved.
