Analysis of the Effect of Fish Bars Made of Bilih Fish (Mystacoleuseus padangensis Blkr) Flour to Reduce Oxidative Stress in a Diabetic Rat Model.

This study aims to analyze the effect of Bilih fish bars (BFB) on oxidative stress based on the levels of MDA and SOD in diabetic rat models. This study used a Randomized Complete Design (RCD). Forty white male rats of the Sprague-Dawley strain were placed into the following five groups: normal and diabetic rats that were fed either the standard feed and metformin, BFB, BFB, and metformin or BFF with a zinc dose of 0.54 mg. A single dose of STZ (40 mg/kg) was used to induce diabetes in the rats. The intervention lasted for 30 d. The differences in MDA and SOD levels between groups were determined with one-way ANOVA followed by Duncan's New Multiple Range Test, and the significance of the statistical level was set at p<0.05. The intervention with BFB and metformin, BFB, and BFF resulted in a decrease in blood glucose levels. The levels of MDA in rats that received the intervention with Bilih fish were 8.236±0.46 µmol/L for the BFB group and 8.266±0.66 µmol/L for the BFF group, which were both lower compared to the control normal rats (8.279±0.51 µmol/L). The levels of SOD in rats that received the intervention with BFB and BFF were higher compared to the diabetic rats with standard feed, but this increase was not significant (p>0.05). BFB and BFF lowered blood sugar levels and decreased the oxidative stress levels based on MDA levels in a diabetic rat model.

Valproic Acid Exposure of Pregnant Rats During Organogenesis Disturbs Pancreas Development in Insulin Synthesis and Secretion of the Offspring.

Valproic acid (VPA) plays a role in histone modifications that eventually inhibit the activity of histone deacetylase (HDAC), and will affect the expressions of genes Pdx1, Nkx6.1, and Ngn3 during pancreatic organogenesis. This experiment was designed to study the effect of VPA exposure in pregnant rats on the activity of HDAC that controls the expression of genes regulating the development of beta cells in the pancreas to synthesize and secrete insulin. This study used 30 pregnant Sprague-Dawley rats, divided into 4 groups, as follows: (1) a control group of pregnant rats without VPA administration, (2) pregnant rats administered with 250 mg VPA on day 10 of pregnancy, (3) pregnant rats administered with 250 mg VPA on day 13 of pregnancy, and (4) pregnant rats administered with 250 mg VPA on day 16 of pregnancy. Eighty-four newborn rats born to control rats and rats administered with VPA on days 10, 13, and 16 of pregnancy were used to measure serum glucose, insulin, DNA, RNA, and ratio of RNA/DNA concentrations in the pancreas and to observe the microscopical condition of the pancreas at the ages of 4 to 32 weeks postpartum with 4-week intervals. The results showed that at the age of 32 weeks, the offspring of pregnant rats administered with 250 mg VPA on days 10, 13, and 16 of pregnancy had higher serum glucose concentrations and lower serum insulin concentrations, followed by decreased concentrations of RNA, and the ratio of RNA/DNA in the pancreas. Microscopical observations showed that the pancreas of the rats born to pregnant rats administered with VPA during pregnancy had low immunoreaction to insulin. The exposure of pregnant rats to VPA during pregnancy disturbs organogenesis of the pancreas of the embryos that eventually disturb the insulin production in the beta cells indicated by the decreased insulin secretion during postnatal life.

The traditional plant, Andrographis paniculata (Sambiloto), exhibits insulin-releasing actions in vitro.

AIM: to examine the effect of A.paniculata on pancreatic b-cells. METHODS: sixty minutes incubation of BRIN-BD11 in Modified Kreb-Ringer Solution containing 16.7 mM glucose (KRB-3) + 0.625 - 2.5 mg/mL A.paniculata evoked 1.7 - 3.73 fold of insulin secretion compared to 16.7 mM glucose only (p = 0.003 - p < 0.001). RESULTS: compared to the effect of 100 mM glibenclamide, 60 minutes incubation of BRIN-BD11 in KRB-3 containing 1.25 and 2.5 mg/mL A. paniculata evoked 1.5 fold (p=0.034) and 2.3 fold (p=0.001) insulin secretion. Twenty minutes incubation of BRIN-BD11 in KRB-3 + 0.625-5 mg/mL A.paniculata, evoked 1.4 - 4.7 fold (p = 0.002 - p < 0.001) of insulin secretion compared to 16.7 mM glucose only. Twenty minutes incubation of BRIN-BD11 in KRB-1 containing 1.11 mM glucose + 0.625 - 10 mg/mL A.paniculata, evoked 1.3 - 3.7 fold (p = 0.019 - p < 0.001) of insulin secretion compared to 16.7 mM glucose only. CONCLUSION: this study conclude that A.paniculata was a very strong, dose dependent insulinotropic agent, glucose dependent and independent insulin secreting agent. This study also conclude that A.paniculata affected one of the membrane receptors, mostly ATP-dependent potassium channels (K+ATP).