Impact of brewery sludge application on heavy metal build-up, translocation, growth and yield of bread wheat (Triticum aestivum L.) crop in Northern Ethiopia.

In a field study, the impact of different levels of brewery sludge (BS) enrichment on Triticum aestivum L. (wheat plants) was examined in terms of growth, yield, heavy metal absorption, and potential health risks linked to plant consumption. Using a randomized complete block design with seven treatments and three blocks, the study showed that applying up to 12 t ha-1 brewery sludge significantly improved all agronomic parameters (except harvest index) compared to control and mineral-fertilized soil. Heavy metal translocation was generally low, except for Cu and Pb. The sequence of heavy metal translocation was Cu > Pb > Cd > Ni > Zn > Mn > Cr from soil to spikes and Cu > Zn > Mn > Pb > Ni > Cd > Cr from soil to grain. Heavy metal loads were mostly higher in roots than in the above-ground crop parts. The target hazard quotient (THQ), hazard index (HI), and target cancer risk (TCR) within wheat grain remained within safe limits for all BS treatments. Consequently, consuming this wheat grain is considered safe regarding heavy metals. Thus, utilizing brewery sludge at 12 t ha-1 as a fertilizer for wheat production and as an alternative method for sludge disposal is plausible.

High-Performance Macroporous Free-Standing Microbial Fuel Cell Anode Derived from Grape for Efficient Power Generation and Brewery Wastewater Treatment.

Microbial fuel cells (MFCs) have the potential to directly convert the chemical energy in organic matter into electrical energy, making them a promising technology for achieving sustainable energy production alongside wastewater treatment. However, the low extracellular electron transfer (EET) rates and limited bacteria loading capacity of MFCs anode materials present challenges in achieving high power output. In this study, three-dimensionally heteroatom-doped carbonized grape (CG) monoliths with a macroporous structure were successfully fabricated using a facile and low-cost route and employed as independent anodes in MFCs for treating brewery wastewater. The CG obtained at 900 °C (CG-900) exhibited excellent biocompatibility. When integrated into MFCs, these units initiated electricity generation a mere 1.8 days after inoculation and swiftly reached a peak output voltage of 658 mV, demonstrating an exceptional areal power density of 3.71 W m-2. The porous structure of the CG-900 anode facilitated efficient ion transport and microbial community succession, ensuring sustained operational excellence. Remarkably, even when nutrition was interrupted for 30 days, the voltage swiftly returned to its original level. Moreover, the CG-900 anode exhibited a superior capacity for accommodating electricigens, boasting a notably higher abundance of Geobacter spp. (87.1%) compared to carbon cloth (CC, 63.0%). Most notably, when treating brewery wastewater, the CG-900 anode achieved a maximum power density of 3.52 W m-2, accompanied by remarkable treatment efficiency, with a COD removal rate of 85.5%. This study provides a facile and low-cost synthesis technique for fabricating high-performance MFC anodes for use in microbial energy harvesting.

Promoting bioremediation of brewery wastewater, production of bioelectricity and microbial community shift by sludge microbial fuel cells using biochar as anode.

Seeking low-cost and eco-friendly electrode catalyst of microbial fuel cell (MFC) reactor has received extensive attention in recent decades. In this study, a sludge MFC was coupled with biochar-modified-anode (BC-300, BC-400, and BC-500) for actual brewery wastewater treatment. The physicochemical properties of biochar largely depended on the pyrolysis temperature, further affecting the removal efficiency of wastewater indicators. BC-400 MFC proved to be efficient for TN and NH4+-N removal, while the maximum removal efficiencies of COD and TP were achieved by BC-500 MFC, reaching respectively 97.14 % and 89.67 %. Biochar could promote the degradation of dissolved organic matter (DOM) in wastewater by increasing the electrochemical performances of MFC. The maximum output voltage of BC-400 MFC reached 410.24 mV, and the maximum electricity generation of 108.05 mW/m2 was also obtained, surpassing the pristine MFC (BCC-MFC) by 4.67 times. High-throughput sequencing results illustrated that the enrichment of electrochemically active bacteria (EAB) and functional bacteria (Longilinea, Denitratisoma, and Pseudomonas) in BC-MFCs, contributed to pollutants degradation and electron transfer. Furthermore, biochar affected directly the electrical conductivity of wastewater, simultaneously changing microbial community composition of MFC anode. Considering both enhanced removal efficiency of pollutants and increased power generation, the results of this study would offer technical reference for the application of biochar as MFC catalyst for brewery wastewater treatment.

Impact of Brewers' Spent Grain-Containing Biscuit on Postprandial Glycaemic Response in Individuals with Metabolic Syndrome: A Crossover Randomised Controlled Trial.

Brewers' spent grain (BSG) is a fibre and protein-rich by-product of beer-brewing. Fermenting BSG with Rhizopus oligosporus can further increase its content of soluble fibre, protein and certain antioxidants. Since nutrients rich in BSG can improve postprandial glycaemic response, this study assessed the postprandial glucose response (PPGR) and postprandial insulin response (PPIR) controlling effect of consuming 30% wheat flour substituted biscuits with autoclaved BSG (ABSG) or fermented BSG (FBSG) in individuals with metabolic syndrome (MetS). The effect on postprandial lipid panel, breath hydrogen (H2) and methane (CH4) concentration and subjective appetite response was also examined. Fifteen subjects with MetS participated in this crossover randomised controlled trial, and blood was collected at 9 time-points for 4 h after consumption of control biscuits (Control), ABSG and FBSG. A significant interaction effect was observed (Pinteraction = 0.013) for the glucose time-points concentration. At 180 min, the glucose concentration was lowered after the consumption of ABSG (p = 0.010) and FBSG (p = 0.012) compared to the Control. Moreover, the FBSG resulted in a significantly lower glucose incremental area under curve (iAUC) compared to the Control (p = 0.028). Insulin level was also lowered at 180 min after the ABSG (p = 0.010) and FBSG (p = 0.051) consumption compared to the Control. However, no difference was noted for postprandial lipid panel, breath H2 and CH4 concentration and subjective appetite response. In conclusion, the consumption of BSG-incorporated biscuits can attenuate PPGR, and fermented BSG incorporation conferred a further PPGR controlling benefit.

Energy audit and associated carbon footprint estimation for a Meta Abo brewery.

Industrial development in Ethiopia is rapidly increasing, leading to a growing gap between energy supply and demand. To address this, efficient energy utilization in existing industries is crucial. Energy audits identify energy losses and recommend saving measures. Therefore, this study evaluates energy efficiency through an audit and estimates greenhouse gas emissions for a Meta Abo brewery. The indirect method of energy audit and the greenhouse gas protocol of carbon footprint estimation were used to evaluate the energy efficiency and carbon emissions of Meta Abo brewery. The boiler efficiency for Bono Energia and Cochran boilers was found to be 79.2% and 80.9%, respectively. Poor insulation caused an estimated annual fuel loss of 35,350 l (638,768 Ethiopian birr) for steam pipes, while steam leakage resulted in a loss of 31,614 l (571,265 Ethiopian birr). The factory's high electricity expense was attributed to a diesel generator consuming 6000 l/d. Greenhouse gas emissions raised from 9156 to 22,697 tons of CO2 equivalent between 2014 and 2018. Implementing the proposed energy-saving measures could save 20.4 TJ of thermal and electrical energy annually, costing approximately 8.5 million Ethiopian birr, and reduce boiler emissions by 455 tons of CO2 equivalent. Therefore, implementation of these measures is recommended.

More than just a beer - Brewers' spent grain, spent hops, and spent yeast as potential functional fillers for polymer composites.

Beer is among the most popular beverages in the world, with the production distributed uniformly between the biggest continents, so the utilization of brewing by-products is essential on a global scale. Among their potential recipients, the plastics industry offers extensive range of potential products. Herein, the presented study investigated the application of currently underutilized solid brewing by-products (brewers' spent grain, spent hops, spent yeast) as fillers for highly-filled poly(ε-caprolactone)-based composites, providing the first direct connection between spent hops or spent yeast and the polymer composites. Comprehensive by-product characterization revealed differences in chemical composition. The elemental C:O ratio, protein content, and Trolox equivalent antioxidant capacity varied from 1.40 to 1.89, 12.9 to 32.4 wt%, and 2.41 to 10.24 mg/g, respectively, which was mirrored in the composites' structure and performance. Morphological analysis pointed to the composition-driven hydrophilicity gap limiting interfacial adhesion for high shares of brewers' spent grain and spent hops, due to high hydrophilicity induced by carbohydrate content. Phytochemicals and other components of applied by-products stimulated composites' oxidative resistance, shifting oxidation onset temperature from 261 °C for matrix over 360 °C for high spent yeast shares. Simultaneously, spent yeast also provided compatibilizing effects for poly(ε-caprolactone)-based composites, reducing complex viscosity compared to other fillers and indicating its highest affinity to poly(ε-caprolactone)due to the lowest hydrophilicity gap. The presented results indicate that the proper selection of brewing by-products and adjustment of their shares creates an exciting possibility of engineering composites' structure and performance, which can be transferred to other polymers differing with hydrophilicity.

A critical review on the biotechnological potential of Brewers' waste: Challenges and future alternatives.

In order to comply with the stringent discharge guidelines issued by governmental organizations to protect the ecosystem, the substantial amounts of effluent and sturdy wastes produced by the beer brewing process need to be discarded or handled in the most affordable and secure manner. Huge quantities of waste material released with each brew bestow a significant opportunity for the brewing sector to move towards sustainability. The concept of circular economy and the development of technological advancements in brewery waste processing have spurred interest to valorize brewery waste for implementation in various sectors of medical and food science, industrial science, and many more intriguing fields. Biotechnological methods for valorizing brewery wastes are showing a path towards green chemistry and are feasible and advantageous to environment. The study unfolds most recent prospectus for brewery waste usage and discusses major challenges with brewery waste treatment and valorization and offers suggestions for further work.

Recovery of Cerium Salts from Sewage Sludge Resulting from the Coagulation of Brewery Wastewater with Recycled Cerium Coagulant.

Due to the high cost and limited sources of cerium coagulants, it is extremely important to take measures to recycle this raw material. This paper presents the new possibility of recovering cerium(III) chloride, cerium(III) sulphate, cerium(IV) sulphate, and potentially phosphate from sewage sludge (101.5 g/kg Ce and 22.2 g/kg total P) through a brewery wastewater treatment process using recycled CeCl3 as a coagulant. In order to recover the Ce and P, the sludge was subjected to extraction using an HCl solution. Optimal process conditions were determined by means of central composite design and response surface methodology (CCD/RSM) for three input parameters (HCl mass, reaction time, and extractant volume). Under optimal conditions (0.35 g HCl per 1 g of sludge, 40 min reaction time, extractant volume of 25 mL per 1 g of sludge), the highest efficiency obtained was 99.6% and 97.5% for Ce and P, respectively. Cerium(III) oxalate as Ce2(C2O4)3∙10H2O was precipitated from the obtained solution using H2C2O4 (99.97%) and decomposed into CeO2 (at 350 °C), which was afterwards subjected to a reaction with HCl (30%, m/m) and H2O2 (30%, m/m), which led to the crystallisation of CeCl3∙7H2O with a purity of 98.6% and a yield of 97.0%. The obtained CeO2 was also subjected to a reaction with H2SO4 (96%, m/m) and H2O2 (30%, m/m), which produced Ce2(SO4)3 with a yield of 97.4%. The CeO2 was also subjected to a reaction with only H2SO4 (96%, m/m), which produced Ce(SO4)2 with a yield of 98.3%. The filtrate obtained after filtering the Ce2(C2O4)3∙10H2O contained 570 mg/L of P, which enabled its use as a source of phosphorus compounds. The presented processes of Ce and potentially P recovery from sewage sludge originating from brewery wastewater contribute to the idea of a circular economy.

Biological recovery of phosphorus (BioP-Rec) from wastewater streams using brewer's yeast on pilot-scale.

Most recent advances for phosphorus (P) recovery using brewery yeast on laboratory scale were used to scale up to a pilot-scale process (BioP-Rec module) and applied in a full-scale wastewater treatment plant (WWTP). A P balance was established for WWTP Markranstädt according to two thresholds: (1) the economic feasibility threshold for P recovery of 0.05 kg/m3 of free P, and (2) the German Sewage Sludge Ordinance (GSSO) threshold, which demands that all WWTPs with a P content in dry matter (DM) of biosolids of 20 gP/kgDM or higher in the coming years must perform mandatory P recovery. In terms of defined thresholds, return and excess sludges were identified as the most feasible WWTP process streams for P recovery. In a 1 m3 BioP-Rec module a 3 stage process was established. From the P-rich water-phase of the return sludge produced in stage 1, which contained 0.051 kg/m3 of free P, 77.56% was taken up by P-depleted brewer's yeast Saccharomyces pastorianus in 3 h in stage 2. In stage 3, the yeast was concentrated in 1 h to produce yeast sludge as a fertilizer product. We demonstrated a novel pilot-scale process for the production of bio-based P-rich fertilizer.

Auto-Brewery Syndrome After COVID-19 Infection.

Auto-brewery syndrome (ABS) is a rare medical condition, wherein gut microbiota ferment carbohydrates to alcohol. Risk factors for ABS include diets high in carbohydrates and sugars, diabetes mellitus, prior gastrointestinal surgery, nonalcoholic fatty liver disease, and certain genetic mutations, among others. We provide a case of ABS that developed after known COVID-19 infection, which may be one of the contributing factors to its development.

Gut and bladder fermentation syndromes: a narrative review.

We recently reported the first clinical case of bladder fermentation syndrome (BFS) or urinary auto-brewery syndrome, which caused the patient to fail abstinence monitoring. In BFS, ethanol is generated by Crabtree-positive fermenting yeast Candida glabrata in a patient with poorly controlled diabetes. One crucial characteristic of BFS is the absence of alcoholic intoxication, as the bladder lumen contains transitional epithelium with low ethanol permeability. In contrast, patients with gut fermentation syndrome (GFS) or auto-brewery syndrome can spontaneously develop symptoms of ethanol intoxication even without any alcohol ingestion because of alcoholic fermentation in the gut lumen. In abstinence monitoring, a constellation of laboratory findings with positive urinary glucose and ethanol, negative ethanol metabolites, and the presence of yeast in urinalysis should raise suspicion for BFS, whereas endogenous ethanol production needs to be shown by a carbohydrate challenge test for GFS diagnosis. GFS patients will also likely fail abstinence monitoring because of the positive ethanol blood testing. BFS and GFS are treated by yeast eradication of fermenting microorganisms with antifungals (or antibiotics for bacterial GFS cases) and modification of underlying conditions (diabetes for BFS and gut dysbiosis for GFS). The under-recognition of these rare medical conditions has led to not only harm but also adverse legal consequences for patients, such as driving under the influence (DUI). GFS patients may be at risk of various alcohol-related diseases.

Xanthan gum and pectin as beverage stabilizers reduce the digestive enzyme hydrolysis of antioxidant and antihypertensive peptides obtained from a brewery byproduct.

An acidic beverage was formulated with xanthan gum (XG), pectin (P) and brewer spent grain (BSG) peptides with antioxidant and antihypertensive properties. The impact of hydrocolloids levels on peptide bioaccessibility was studied. Peptides were obtained from BSG using Purazyme and Flavourzyme enzymes. BSG peptides were fractionated by ultrafiltration (UF) and four fractions were obtained: F1 (>10 kDa), F2 (10-5 kDa), F3 (1-5 kDa), and F4 (<1 kDa). F3 showed the highest protein purity, ferulic acid content, proportion of amphipathic peptides, and bioactive properties (ABTS+ radical scavenging and ACE-I inhibitory activity). The identified peptides from F3 by tandem mass spectrometry were 138. In silico analysis showed that 26 identified peptides had ABTS+ inhibitory activity, while 59 ones presented good antihypertensive properties. The effect of XG and P levels on bioaccessibility of F3 peptides in the formulated beverages was studied by a central composite experimental design. It was observed that F3 peptides interacted with hydrocolloids by electrostatic forces at pH of formulated beverages. The addition of hydrocolloids to formulation modulated the release of the antioxidant peptides and protected the degradation of ACE-I inhibitory peptides from F3 during simulated gastrointestinal digestion. Finally, the level of hydrocolloids that produced intermediate viscosities in the formulated beverages improved the bioaccessibility of the F3 peptides.

Implementation of the water footprint as a water performance indicator in industrial manufacturing units located in Greece: challenges and prospects.

Global water resources are under great pressure, so sustainable water and wastewater management is essential for every industrial unit. The water footprint (WF) is a multidimensional indicator of volumetric water use and pollution and is applied throughout the production cycle from the supply chain to the end user. The state-of-the art method of calculating the direct (operational) WF requires detailed records of water consumption and wastewater production per process, that are usually not available. In order to effectively implement the indicator, modifications to the standard calculation method are usually necessary, to meet the requirements and characteristics of each production unit. The present study focuses in three production units in Greece (i.e., cement, brewery, and natural cosmetics) and analyzes the modifications and assumptions required for the operational WF assessment, calculated for each production process where possible and for the whole unit. The WF assessment performed for the three case studies showed that both water consumption rates and operational WFs are within the lowest values (4.7 hL water/hL beer for brewery case study and 0.155 L/kg cement for cement case study) of the ranges reported in the international literature (4-8.8 hL water/hL beer and 0.14-0.413 L/kg cement). The total operational WF of the brewery after applying mass balances was calculated at 23.8 hL water/hL beer with virtual grey WF contributing at 79.7% and blue WF at 20.3%. For the cement manufacturing unit, using estimations from the plant operators and data from international literature, the total operational WF of the plant was assessed at 0.159 L/kg cement with blue WF contributing at 95.8%, green WF at 1%, and virtual grey WF at 3.2%. For the natural cosmetics unit, after modifying the system boundaries properly, the total operational WF was assessed at 81.6 L/kg bulk with virtual grey WF contributing at 88.8%. Through WF assessment, strategic actions could be identified to reduce water consumption and a benchmark could be provided to assess water performance between companies of the same branch.

Biorefinery of brewery spent grain to obtain bioproducts with high value-added in the market.

The brewery industry is under economic and environmental pressure to minimize residual management costs, particularly brewery spent grain (BSG), which accounts for 80-85% (w/w) of the total by-products generated. BSG is a lignocellulosic material primarily composed of carbohydrates, proteins and lipids. Developing a biorefinery model for conversion of BSG into value-added products is a plausible idea. Previous work optimized the pretreatment of BSG with the ionic liquid [N1112OH][Gly] and further release of fermentable sugar-containing solutions by enzymatic hydrolysis, using an enzymatic cocktail obtained by solid-state fermentation of BSG with Aspergillus brasiliensis CECT 2700 and Trichoderma reesei CECT 2414. The current work ends the biorefinery process, studying the fermentation of these culture media with two LAB strains, Lactobacillus pentosus CECT 4023 and Lactobacillus plantarum CECT 221, from which the production of organic acids, bacteriocins, and microbial biosurfactants (mBS) was obtained. In addition to the bacteriocin activity observed, a mass balance of the whole biorefinery process quantified the production of 106.4 g lactic acid and 6.76 g mBS with L. plantarum and 116.1 g lactic acid and 4.65 g mBS with L. pentosus from 1 kg of dry BSG. Thus, BSG shows a great potential for waste valorization, playing a major role in the implementation of biomass biorefineries in circular bioeconomy.

Data on the effects of brewery sludge and blended NPS fertilizer rates on yield and yield components of maize (Zea Mays L.) in North Mecha District, Northwestern Ethiopia.

Maize is one of the most important cereal crops in Ethiopia. However, its yield is lower than its potential mainly due to low soil fertility problems. Therefore, this field experiment was conducted during 2021 main rainy season with the objective of improving maize production through application of brewery sludge and blended NPS fertilizers in North Mecha district, Northwestern Ethiopia. Factorial combination of three levels of brewery sludge (0, 10 and 20 t ha-1) and four levels of blended NPS fertilizer rates (0, 50, 100 and 150 kg ha-1) were laid out in RCBD with three replications. The remaining necessary agronomic practices and crop management activities were undertaken uniformly. The data presented under this dataset article includes phenological Parameters (i.e. Days to 50 % emergence, Days to 50 % tasseling, Days to 50 % silking and Days to 90 % physiological maturity), growth parameters (i.e. Plant height, leaf area and leaf area index) and yield and yield component parameters (i.e. Number of ears plant-1, Above ground dry biomass yield, Ear length, Number of rows ear-1, Number of kernels row-1, Number of kernels ear-1, Grain yield, Thousand kernels weight, Stover yield and Harvest index). All the collected data were subjected to analysis of variance (ANOVA) and the analysis was carried out using the SAS version 9.0 software computer program's General Linear Model (GLM) procedure [1]. As described in Montgomery [2], the residuals were examined to verify the normal distribution and homogeneous variance model assumptions on the error terms for each response variable. Because the twelve treatment combinations were randomized within each block, the independence assumption is valid. When a treatment effect was significant, multiple means comparison was performed at a 5 % level of significance using the least significant difference (Fisher's LSD) method to generate letter groupings and correlation analysis was performed using the Pearson correlation procedure found in SAS. This dataset article, therefore gives information about the effects of brewery sludge and blended NPS fertilizer rates on maize productivity. Additionally, it provides the economic feasibility of brewery sludge integrated application with blended NPS fertilizer than sole application of blended chemical fertilizers. Hence, this information can allow other researchers to review the supplement data, methods, and make detailed analysis, which possibly giving rise to new lines of inquiry. This can also give rise to new collaborations and boost the reputation of the present research data within the scientific community and to make it available to everyone around the subject matter to use as they wish.

Current strategies for the management of valuable compounds from hops waste for a circular economy.

World beer production generates large volumes of waste discharged with every brew. Recently, new methods of reducing and reusing hops waste: hot trub (HT), and brewer-spent hops (BSH) are being exploited to improve the circular economy processes. This review outlines the current achievements in the management of hops waste. Following an in-depth review of various scientific publications, current strategies are discussed as a sustainable alternative to food waste exploitation and an inexpensive source of valuable compounds. Moreover, key aspects concerning the nutritional value of hops waste and the potential to enhance the functional properties of food and beverages are highlighted. Due to their nutritional composition, hops residues may be used as prospective sources of added-value co-products or additives for food enrichment, especially for products rich in fat, or as a new source of vegetable protein.

The Associations of Auto-Brewery Syndrome and Diabetes Mellitus: A Literature Review and Clinical Perspective.

Endogenous production of alcohol without the external intake of alcohol is called auto-brewery syndrome (ABS), and to get its levels to rise to a level that it has physical symptoms of alcohol intake is rare. The most common cause of ABS is the metabolism of ingested carbohydrates by intestinal microflora. This occurrence does not happen in all normal individuals but only in some high-risk individuals. Patients with diabetes mellitus (DM) have been hypothesized to be at high risk for ABS. We searched databases, such as PubMed, Medline, and PubMed Central, to search for existing literature with relevant keywords. In the finalized review, we have included 30 relevant articles. Alcohol formed in the gut gets absorbed in the bloodstream and immediately gets metabolized, so usually it does not achieve a level in blood high enough to cause symptoms. In high-risk patients, there is an increase in the level of bloodstream alcohol above a certain level, so it shows symptoms. Because there is higher blood glucose in DM, the patients have been shown to be at increased risk for developing ABS. Similarly, obesity is also a risk factor for DM, making it a high-risk condition for ABS. The most involved pathogens are Candida and Saccharomyces.

Do microbes evade domestication? - Evaluating potential ferality among diastatic Saccharomyces cerevisiae.

Certain lineages of the wine, beer and bread yeast Saccharomyces cerevisiae have diastatic activity. They contain the chimeric gene STA1 that codes for an extracellular glucoamylase which enables the strains to degrade starch and dextrins. Beer contaminations by diastatic yeasts can be dangerous because they can cause super-attenuation due to the consumption of otherwise non-fermentable oligosaccharides, gushing and off-flavours. Given that diastatic yeasts can be used for beer fermentation it is important to understand the relationship between production and contaminant strains, their natural reservoirs and entry routes into the brewery. Here, we analyze real cases of contamination in a Portuguese craft brewery over a period of 18 months. By analyzing with whole genome sequencing several contaminants, we show that recurrent contaminations by diastatic yeasts are caused by environmental strains. Moreover, some beer contaminants were closely related to diastatic environmental strains isolated in Botswana. We observed the widespread presence of domestication signatures in diastatic strains. Moreover, the combined phylogeny of STA1 and its ancestor, SGA1, suggested a single STA1 origin, as ancient as the entire lineage of diastatic yeasts. Together, our results suggest that diastatic yeasts isolated in natural settings could be escaping from domestication settings and becoming feral.

Bacterial community dynamics and associated genes in hydrocarbon contaminated soil during bioremediation using brewery spent grain.

Brewery spent grain (BSG) has previously been exploited in bioremediation. However, detailed knowledge of the associated bacterial community dynamics and changes in relevant metabolites and genes over time is limited. This study investigated the bioremediation of diesel contaminated soil amended with BSG. We observed complete degradation of three total petroleum hydrocarbon (TPH C10-C28) fractions in amended treatments as compared to one fraction in the unamended, natural attenuation treatments. The biodegradation rate constant (k) was higher in amended treatments (0.1021k) than in unamended (0.059k), and bacterial colony forming units increased significantly in amended treatments. The degradation compounds observed fitted into the elucidated diesel degradation pathways and quantitative PCR results showed that the gene copy numbers of all three associated degradation genes, alkB, catA and xylE, were significantly higher in amended treatments. High-throughput sequencing of 16S rRNA gene amplicons showed that amendment with BSG enriched autochthonous hydrocarbon degraders. Also, community shifts of the genera Acinetobacter and Pseudomonas correlated with the abundance of catabolic genes and degradation compounds observed. This study showed that these two genera are present in BSG and thus may be associated with the enhanced biodegradation observed in amended treatments. The results suggest that the combined evaluation of TPH, microbiological, metabolite and genetic analysis provides a useful holistic approach to assessing bioremediation.

Assessing the potential of Chlorella sp. phycoremediation liquid digestates from brewery wastes mixture integrated with bioproduct production.

Digestates from different anaerobic digesters are promising substrates for microalgal culture, leading to effective wastewater treatment and the production of microalgal biomass. However, further detailed research is needed before they can be used on a large scale. The aims of this study were to investigate the culture of Chlorella sp. in DigestateM from anaerobic fermentation of brewer's grains and brewery wastewater (BWW) and to explore the potential use of the biomass produced under different experimental conditions, including diverse cultivation modes and dilution ratios. Cultivation in DigestateM initiated from 10% (v/v) loading, with 20% BWW, obtained maximum biomass production, reaching 1.36 g L-1 that was 0.27g L-1 higher than 1.09 g L-1 of BG11. In terms of DigestateM remediation, the maximum removal of ammonia nitrogen (NH4 +-N), chemical oxygen demand, total nitrogen, and total phosphorus reached 98.20%, 89.98%, 86.98%, and 71.86%, respectively. The maximum lipid, carbohydrate, and protein contents were 41.60%, 32.44%, and 27.72%, respectively. The growth of Chlorella sp. may be inhibited when the Y(II)-Fv/Fm ratio is less than 0.4.