Differential warming at crown scale impacts walnut primary growth onset and secondary growth rate.

Trees are exposed to significant spatio-temporal thermal variations, which can induce intracrown discrepancies in the onset and dynamics of primary and secondary growth. In recent decades, an increase in late winter and early spring temperatures has been observed, potentially accelerating bud break, cambial activation and their potential coordination. Intracrown temperature heterogeneities could lead to asymmetric tree shapes unless there is a compensatory mechanism at the crown level. An original warming experiment was conducted on young Juglans regia trees in a greenhouse. From February to August, the average temperature difference during the day between warmed and control parts was 4°C. The warming treatment advanced the date of budbreak significantly, by up to 14 days. Warming did not alter secondary growth resumption but increased growth rates, leading to higher xylem cell production (twice as many) and to an increase in radial increment (+80% compared to control). Meristems resumptions were asynchronous without coordination in response to temperature. Buds on warmed branches began to swell two weeks prior to cambial division, which was one week earlier than on control branches. A difference in carbon and water remobilisation at the end of bud ecodormancy was noted under warming. Overall, our results argue for a lack of compensatory mechanisms at the crown scale, which may lead to significant changes in tree architecture in response to intra-crown temperature heterogeneities.

Identifying indicators of apple bud dormancy status by exposure to artificial forcing conditions.

Dormancy in temperate fruit trees is a mechanism of temporary growth suspension, which is vital for tree survival during winter. Studies on this phenomenon frequently employ scientific methods that aim to detect the timing of dormancy release. Dormancy release occurs when trees have been exposed to sufficient chill, allowing them to resume growth under conducive conditions. This study investigates dormancy dynamics in two apple (Malus × domestica Borkh.) cultivars, 'Nicoter' and 'Topaz', by sampling branches in an orchard over 14 weeks (2019-2020) and over 31 weeks (2021-2022) and subjecting them to a 42-day budbreak forcing period in a growth chamber. Temporal changes in budbreak percentages demonstrated dormancy progression in the studied apple cultivars and allowed distinguishing the three main dormancy phases: paradormancy (summer dormancy), endodormancy (deep dormancy), and ecodormancy (spring dormancy), along with transition periods between them. Using these data, we explored the suitability of several alternative methods to determine endodormancy release. Tabuenca's test, which predicts dormancy release based on the differences in dry weights of buds with and without forcing, showed promise for this purpose. However, our data indicated a need for considerable adjustments and validation of this test. Bud weight and water content of buds in the orchard did not align with budbreak percentages under forcing conditions, rendering them unsuitable for determining endodormancy release in 'Nicoter' and 'Topaz'. Shoot growth cessation did not seem to be connected with either dormancy progression or dormancy depth of the studied cultivars, whereas leaf fall coincided with the beginning of the transition from endo- to ecodormancy. This work addresses methodological limitations in dormancy research and suggests considering the mean time to budbreak and budbreak synchrony as additional criteria to assess tree dormancy status.

A Hierarchical Model to Predict Time of Flowering of Kiwifruit Using Weather Data and Budbreak Dynamics.

Accurate prediction of flowering times is essential for efficient orchard management for kiwifruit, facilitating timely pest and disease control and pollination interventions. In this study, we developed a predictive model for flowering time using weather data and observations of budbreak dynamics for the 'Hayward' and 'Zesy002' kiwifruit. We used historic data of untreated plants collected from 32 previous studies conducted between 2007 and 2022 and analyzed budbreak and flowering timing alongside cumulative heat sum (growing degree days, GDDs), chilling unit (CU) accumulation, and other environmental variables using weather data from the weather stations nearest to the study orchards. We trained/parameterized the model with data from 2007 to 2019, and then evaluated the model's efficacy using testing data from 2020 to 2022. Regression models identified a hierarchical structure with the accumulation of GDDs at the start of budbreak, one of the key predictors of flowering time. The findings suggest that integrating climatic data with phenological events such as budbreak can enhance the predictability of flowering in kiwifruit vines, offering a valuable tool for kiwifruit orchard management.

Cold hardiness-informed budbreak reveals role of freezing temperatures and daily fluctuation in chill accumulation model.

Fundamental questions in bud dormancy remain, including what temperatures fulfill dormancy requirements (i.e., chill accumulation). Recent studies demonstrate freezing temperatures promote chill accumulation and cold hardiness influences time to budbreak - the phenotype used for dormancy evaluations. Here we evaluated bud cold hardiness (CH) and budbreak responses of grapevines (Vitis hybrids) throughout chill accumulation under three treatments: constant (5 °C), fluctuating (-3.5 to 6.5 °C daily), and field conditions (Madison, WI, USA). Chill treatments experiencing lower temperatures promoted greater gains in cold hardiness (CHfield>CHfluctuating>CHconstant). All treatments decreased observed time to budbreak with increased chill accumulation. However, perceived treatment effectiveness changed when time to budbreak was adjusted to remove cold acclimation effects. Among three classic chill models (North Carolina, Utah, and Dynamic), none were able to correctly describe adjusted time to budbreak responses to chill accumulation. Thus, a new model is proposed that expands the range of chill accumulation temperatures to include freezing temperatures and enhances chill accumulation under fluctuating temperature conditions. Most importantly, our analysis demonstrates adjustments for uneven acclimation change the perceived effectiveness of chill treatments. Therefore, future work in bud dormancy would benefit from simultaneously evaluating cold hardiness.

Genetic and molecular regulation of chilling requirements in pear: breeding for climate change resilience.

Pear (Pyrus spp.) is a deciduous fruit tree that requires exposure to sufficient chilling hours during the winter to establish dormancy, followed by favorable heat conditions during the spring for normal vegetative and floral budbreak. In contrast to most temperate woody species, apples and pears of the Rosaceae family are insensitive to photoperiod, and low temperature is the major factor that induces growth cessation and dormancy. Most European pear (Pyrus Communis L.) cultivars need to be grown in regions with high chilling unit (CU) accumulation to ensure early vegetative budbreak. Adequate vegetative budbreak time will ensure suitable metabolite accumulation, such as sugars, to support fruit set and vegetative development, providing the necessary metabolites for optimal fruit set and development. Many regions that were suitable for pear production suffer from a reduction in CU accumulation. According to climate prediction models, many temperate regions currently suitable for pear cultivation will experience a similar accumulation of CUs as observed in Mediterranean regions. Consequently, the Mediterranean region can serve as a suitable location for conducting pear breeding trials aimed at developing cultivars that will thrive in temperate regions in the decades to come. Due to recent climatic changes, bud dormancy attracts more attention, and several studies have been carried out aiming to discover the genetic and physiological factors associated with dormancy in deciduous fruit trees, including pears, along with their related biosynthetic pathways. In this review, current knowledge of the genetic mechanisms associated with bud dormancy in European pear and other Pyrus species is summarized, along with metabolites and physiological factors affecting dormancy establishment and release and chilling requirement determination. The genetic and physiological insights gained into the factors regulating pear dormancy phase transition and determining chilling requirements can accelerate the development of new pear cultivars better suited to both current and predicted future climatic conditions.

Target enrichment sequencing coupled with GWAS identifies MdPRX10 as a candidate gene in the control of budbreak in apple.

The timing of floral budbreak in apple has a significant effect on fruit production and quality. Budbreak occurs as a result of a complex molecular mechanism that relies on accurate integration of external environmental cues, principally temperature. In the pursuit of understanding this mechanism, especially with respect to aiding adaptation to climate change, a QTL at the top of linkage group (LG) 9 has been identified by many studies on budbreak, but the genes underlying it remain elusive. Here, together with a dessert apple core collection of 239 cultivars, we used a targeted capture sequencing approach to increase SNP resolution in apple orthologues of known or suspected A. thaliana flowering time-related genes, as well as approximately 200 genes within the LG9 QTL interval. This increased the 275 223 SNP Axiom® Apple 480 K array dataset by an additional 40 857 markers. Robust GWAS analyses identified MdPRX10, a peroxidase superfamily gene, as a strong candidate that demonstrated a dormancy-related expression pattern and down-regulation in response to chilling. In-silico analyses also predicted the residue change resulting from the SNP allele associated with late budbreak could alter protein conformation and likely function. Late budbreak cultivars homozygous for this SNP allele also showed significantly up-regulated expression of C-REPEAT BINDING FACTOR (CBF) genes, which are involved in cold tolerance and perception, compared to reference cultivars, such as Gala. Taken together, these results indicate a role for MdPRX10 in budbreak, potentially via redox-mediated signaling and CBF gene regulation. Moving forward, this provides a focus for developing our understanding of the effects of temperature on flowering time and how redox processes may influence integration of external cues in dormancy pathways.

Time to budbreak is not enough: cold hardiness evaluation is necessary in dormancy and spring phenology studies.

BACKGROUND: Dormancy of buds is an important phase in the life cycle of perennial plants growing in environments where unsuitable growth conditions occur seasonally. In regions where low temperature defines these unsuitable conditions, the attainment of cold hardiness is also required for survival. The end of the dormant period culminates in budbreak and flower emergence, or spring phenology, one of the most appreciated and studied phenological events - a time also understood to be most sensitive to low-temperature damage. Despite this, we have a limited physiological and molecular understanding of dormancy, which has negatively affected our ability to model budbreak. This is also true for cold hardiness. SCOPE: Here we highlight the importance of including cold hardiness in dormancy studies that typically only characterize time to budbreak. We show how different temperature treatments may lead to increases in cold hardiness, and by doing so also (potentially inadvertently) increase time to budbreak. CONCLUSIONS: We present a theory that describes evaluation of cold hardiness as being key to clarifying physiological changes throughout the dormant period, delineating dormancy statuses, and improving both chill and phenology models. Erroneous interpretations of budbreak datasets are possible by not phenotyping cold hardiness. Changes in cold hardiness were very probably present in previous experiments that studied dormancy, especially when those included below-freezing temperature treatments. Separating the effects between chilling accumulation and cold acclimation in future studies will be essential for increasing our understanding of dormancy and spring phenology in plants.

Significant increases in Donghong kiwifruit yield by a novel umbrella-shaped trellis system and identification of associated molecular mechanisms.

China is the largest kiwifruit producer in the world, accounting for more than half of the total. However, in terms of yield per unit area, China is much lower than the global average and lags behind that of other countries. Yield improvement is of critical importance for the current kiwifruit industry in China. In this study, an improved overhead pergolas trellis (OPT) system, namely, the umbrella-shaped trellis (UST) system, was developed for Donghong kiwifruit, which is now the second most popular and widely cultivated red-fleshed kiwifruit in China. Surprisingly, the estimated yield on the UST system was more than two times higher than that with a traditional OPT, while the external fruit quality was maintained and the internal fruit quality was improved. One of the mechanisms contributing to the yield improvement was the significant promotion of the vegetative growth of canes at 6 ~ 10 mm in diameter by the UST system. The upper canopy of the UST treatment served as a natural shading condition for the lower fruiting canopy and thus had positive effects on the accumulation of chlorophylls and total carotenoids in the fruiting canopy. The most productive zones on the fruiting canes (6 ~ 10 mm in diameter) contained significantly higher (P < 0.05) levels of zeatin riboside (ZR) and auxin (IAA) and ratios of ZR/gibberellin (GA), ZR/abscisic acid (ABA), and ABA/GA. A relatively high carbon/nitrogen ratio may promote the flower bud differentiation process of Donghong kiwifruit. The outcomes of this study provide a scientific basis for manifold increase in production of kiwifruit and contribute to the sustainability of the kiwifruit industry.

Assisted migration is plausible for a boreal tree species under climate change: A quantitative and population genetics study of trembling aspen (Populus tremuloides Michx.) in western Canada.

A novel method was tested for improving tree breeding strategies that integrate quantitative and population genetics based on range-wide reciprocal transplant experiments. Five reciprocal common garden tests of Populus tremuloides were investigated including 6450 trees across western Canada focusing on adaptation traits and growth. Both genetic parameters and home-site transplant models were evaluated. We found a genetic trade-off between growth and early spring leaf flush and late fall senescence. Coefficients of phenotypic variation (CVp) of cell lysis (CL), a measure of freezing injury, shrank from 0.28 to 0.10 during acclimation in the fall, and the CVp slope versus the freezing temperature was significantly different from zero (R 2 = 0.33, p = .02). There was more between-population genetic variation in fall phenology than in spring leaf phenology. We suggest that P. tremuloides demonstrated a discrepancy between the ecological optimum and the physiological optimum minimum winter temperature. The sub-optimal growing condition of P. tremuloides is potentially caused by the warmer ecological optimum than the physiological optimum. Assisted migration and breeding of fast growers to reforest cooler plantation sites can improve productivity. Transferring the study populations to less than 4°C of extreme minimum temperature appears safe for reforestation aligning with the historical recolonization direction of the species. This is equivalent to a 5-10° latitudinal northward movement. Fall frost hardiness is an effective criterion for family selection in the range tested in this study.

Woody species do not differ in dormancy progression: Differences in time to budbreak due to forcing and cold hardiness.

Budbreak is one of the most observed and studied phenological phases in perennial plants, but predictions remain a challenge, largely due to our poor understanding of dormancy. Two dimensions of exposure to temperature are generally used to model budbreak: accumulation of time spent at low temperatures (chilling) and accumulation of heat units (forcing). These two effects have a well-established negative correlation; with more chilling, less forcing is required for budbreak. Furthermore, temperate plant species are assumed to vary in chilling requirements for dormancy completion allowing proper budbreak. Here, dormancy is investigated from the cold hardiness standpoint across many species, demonstrating that it should be accounted for to study dormancy and accurately predict budbreak. Most cold hardiness is lost prior to budbreak, but rates of cold hardiness loss (deacclimation) vary among species, leading to different times to budbreak. Within a species, deacclimation rate increases with accumulation of chill. When inherent differences between species in deacclimation rate are accounted for by normalizing rates throughout winter by the maximum rate observed, a standardized deacclimation potential is produced. Deacclimation potential is a quantitative measurement of dormancy progression based on responsiveness to forcing as chill accumulates, which increases similarly for all species, contradicting estimations of dormancy transition based on budbreak assays. This finding indicates that comparisons of physiologic and genetic control of dormancy require an understanding of cold hardiness dynamics. Thus, an updated framework for studying dormancy and its effects on spring phenology is suggested where cold hardiness in lieu of (or in addition to) budbreak is used.

Phenology explains different storage remobilization in two congeneric temperate tree species with contrasting leaf habit.

The dependence of trees on carbon and nutrient storage is critical to predicting the forest vulnerability under climate change, but whether evergreen and deciduous species differ in their use and allocation of stored resources during spring phenology is unclear. Using a high temporal resolution, we evaluated the role of spring phenology and shoot growth as determinants of the carbon and nutrient storage dynamics in contrasting leaf habits. We recorded the phenology and shoot elongation and determined the concentrations of total non-structural carbohydrates (NSCs), starch, soluble carbohydrates, nitrogen (N) and phosphorus (P) in buds, expanding shoots and previously formed shoots of two sympatric Nothofagus species with contrasting leaf habit. Species reached similar shoot lengths, though shoot expansion started 35 days earlier and lasted c. 40 days more in the deciduous species. Thus, although the deciduous species had a relatively constant shoot growth rate, the evergreen species experienced a conspicuous growth peak for c. 20 days. In the evergreen species, the greatest decreases in NSC concentrations of previously formed shoots and leaves coincided with the maximum shoot expansion rate and fruit filling, with minimums of 63 and 65% relative to values at bud dormancy, respectively. In contrast, minimum NSC concentrations of the previously formed shoots of the deciduous species were only 73% and occurred prior to the initiation of shoot expansion. Bud N and P concentrations increased during budbreak, whereas previously formed shoots generally did not decrease their nutrient concentrations. Late spring phenology and overlapping of phenophases contributed to the greater dependence on storage of proximal tissues in the studied evergreen compared with deciduous species, suggesting that phenology is a key determinant of the contrasting patterns of storage use in evergreen and deciduous species.

Water Regime Monitoring of the Royal Walnut (Juglans regia L.) Using Sap Flow and Dendrometric Measurements.

Changes in the distribution of annual rainfall totals, together with the increase in temperature over the last 40 years, are causing more frequent periods of drought, and plants are more often exposed to water stress. The aim of this study was to monitor the effect of different water regimes (irrigated and non-irrigated) of individuals of walnut tree (Juglans regia L.) in a private orchard located in the West of Slovakia. Our research was focused on dendrometric and sap flow measurements in the period from 28 March to 2 June 2019. The results showed differences in the sap flow of walnut trees during the budbreak period: when trees were irrigated, sap flow in the diurnal cycle was around 130 g·h-1 (20.48%), higher than in the non-irrigated treatment. Dendrometric differences between the irrigated and non-irrigated treatments were not significant. The sap flow data in the flowering period of the irrigated variant were slightly higher at 150 g·h-1 (35.62%) than non-irrigated. Dendrometric differences were more significant when the difference between the variants was more than 1.5 mm. Continuation of this research and analysis of the data obtained in the coming years will allow us to evaluate the effects of the environment on fruit trees in the long term.

Unraveling the role of MADS transcription factor complexes in apple tree dormancy.

A group of MADS transcription factors (TFs) are believed to control temperature-mediated bud dormancy. These TFs, called DORMANCY-ASSOCIATED MADS-BOX (DAM), are encoded by genes similar to SHORT VEGETATIVE PHASE (SVP) from Arabidopsis. MADS proteins form transcriptional complexes whose combinatory composition defines their molecular function. However, how MADS multimeric complexes control the dormancy cycle in trees is unclear. Apple MdDAM and other dormancy-related MADS proteins form complexes with MdSVPa, which is essential for the ability of transcriptional complexes to bind to DNA. Sequential DNA-affinity purification sequencing (seq-DAP-seq) was performed to identify the genome-wide binding sites of apple MADS TF complexes. Target genes associated with the binding sites were identified by combining seq-DAP-seq data with transcriptomics datasets obtained using a glucocorticoid receptor fusion system, and RNA-seq data related to apple dormancy. We describe a gene regulatory network (GRN) formed by MdSVPa-containing complexes, which regulate the dormancy cycle in response to environmental cues and hormonal signaling pathways. Additionally, novel molecular evidence regarding the evolutionary functional segregation between DAM and SVP proteins in the Rosaceae is presented. MdSVPa sequentially forms complexes with the MADS TFs that predominate at each dormancy phase, altering its DNA-binding specificity and, therefore, the transcriptional regulation of its target genes.

Hydrogen Peroxide Increases during Endodormancy and Decreases during Budbreak in Grapevine (Vitis vinifera L.) Buds.

Changes in the level of hydrogen peroxide (H2O2) is a good indicator to monitor fluctuations in cellular metabolism and in the stress responses. In this study, the changes in H2O2 content during bud endodormancy (ED) and budbreak were analysed in grapevine (Vitis vinifera L.). The results showed a gradual increase in the H2O2 content during the development of bud ED, which was mainly due to an increase in the activity of peroxidases (PODs). The maximum H2O2 content reached in the grapevine buds coincided with the maximum depth of bud ED. In contrast, during budbreak, the H2O2 content decreased. As the plant hormones cytokinin (CK) and auxin play an important role in budbreak and growth resumption in grapevine, the effect of exogenous applications of H2O2 on the expression of genes involved in CK and auxin metabolism was analysed. The results showed that H2O2 represses the expression of the CK biosynthesis genes VvIPT3a and VvLOG1 and induces the expression of the CK-inactivating gene VvCKX3, thus reducing potentially the CK content in the grapevine bud. On the other hand, H2O2 induced the expression of the auxin biosynthesis genes VvAMI1 and VvYUC3 and of the auxin transporter gene VvPIN3, thus increasing potentially the auxin content and auxin transport in grapevine buds. In general, the results suggest that H2O2 in grapevine buds is associated with the depth of ED and negatively regulates its budbreak.

RNAi-mediated repression of dormancy-related genes results in evergrowing apple trees.

DORMANCY-ASSOCIATED MADS-box (DAM) and SHORT VEGETATIVE PHASE (SVP) genes have been implicated in the regulation of winter dormancy in perennials. Ectopic expression of apple (Malus × domestica Borkh. 'Royal Gala') DAM and SVP genes delays budbreak and constrains lateral shoot outgrowth. In this study, we used RNA interference (RNAi) to simultaneously target all apple DAM and SVP genes in order to study their role and mode of action in the regulation of bud dormancy, budbreak and flowering. A synthetic construct carrying a hairpin fragment assembled from sequences specific to coding regions of three DAM and two SVP genes was used to generate transgenic lines. Reduced expression of DAM/SVP genes resulted in delayed leaf senescence and abscission in autumn, failure to enter bud dormancy in winter and continual growth of new leaves regardless of the season for over 3 years. Precocious flowering but normal flower morphology, fertility and fruit development were observed. The non-dormant phenotype was associated with modified phytohormone composition. The content of gibberellins (GAs) and jasmonates (JAs) was significantly increased in terminal buds of RNAi lines compared with wildtype plants, accompanied by elevated expression of the key GA biosynthesis pathway gene GIBBERELLIN 20 OXIDASE-2 (MdGA20ox-2) along with the FLOWERING LOCUS T gene MdFT2. The key mediator of plasmodesmatal closure, MdCALLOSE SYNTHASE 1 (MdCALS1), was repressed in RNAi lines. This study provides functional evidence for the role of DAM/SVP genes in vegetative phenology of apple and paves the way for production of low-chill varieties suitable for growth in warming climates.

Dormancy of Floral Buds of Rabbiteye Blueberry in a Mild Winter Climate

Abstract Blueberry is an important fruit crop, with many health benefits. Despite its importance, much remains to be studied concerning the dormancy dynamics in rabbiteye cultivars growing in a mild winter climate. In this research, the dormancy in blueberry, rabbiteye cultivars 'Bluegem', 'Climax', 'Delite', and 'Powderblue', was studied in a mild winter region. The single-node cuttings biological test and the evaluation of the hydric status were performed in dormant winter reproductive buds. These experiments were performed during fall and winter in one year (2016). Moreover, chilling hours under or equal to 7.2 ºC were measured, and chill units were calculated according to Utah Model [1], Modified Utah Model [2], and Blueberry Model [3]. In conclusion, the four cultivars showed a similar pattern, revealing a dormant state in the initial sampling dates and a released dormancy in the final treatments, showing the decrease of dormancy in June and July. However, Delite was earlier than the other cultivars. Bluegem and Delite required 134.0 chilling hours, 127.0 chill units (Utah Model), 198.5 chill units (Modified Utah Model), and 971.5 chill units (Blueberry Model) for 50% of their green tip buds reach the opened bud stage. Climax required 44.0, -11.0, 56.5, and 440.5, respectively. And Powderblue required 44.0, 5.5, 77.0, and 725.0 respectively. This study can bring some insights into crop management and production of this important fruit crop, especially in a global climate-changing scenario, related to flowering and dormancy control, as well as helping to select suitable cultivars to a region, concerning chilling requirements.

Tempo of gene regulation in wild and cultivated Vitis species shows coordination between cold deacclimation and budbreak.

Dormancy release, loss of cold hardiness and budbreak are critical aspects of the annual cycle of deciduous perennial plants. Molecular control of these processes is not fully understood, and genotypic variation may be important for climate adaptation. To gain greater understanding of these processes, single-node cuttings from wild (Vitis amurensis, V. riparia) and cultivated Vitis genotypes (V. vinifera 'Cabernet Sauvignon', 'Riesling') were collected from the vineyard during winter and placed under forcing conditions. Cold hardiness was measured daily, and buds were collected for gene expression analysis until budbreak. Wild Vitis genotypes had faster deacclimation and budbreak than V. vinifera. Temperature-sensing related genes were quickly and synchronously differentially expressed in all genotypes. Significant changes in the pattern of expression changes for eight major metabolic and hormone related pathways were seen across all genotypes. Downregulation of ABA synthesis appears to play an important role in loss of cold hardiness and budbreak in all genotypes. This role was validated through an observed halt in cold hardiness loss of 'Riesling' buds treated with exogenous ABA. The gene expression cascade that occurs during deacclimation and budbreak phenology of fast (wild) and slow (cultivated) grapevines appears coordinated and temporally conserved within these phenotypes.

Histone modification and activation by SOC1-like and drought stress-related transcription factors may regulate AcSVP2 expression during kiwifruit winter dormancy.

The SHORT VEGETATIVE PHASE (SVP)-like and DORMANCY ASSOCIATED MADS-BOX (DAM) genes have been shown to regulate winter dormancy in woody perennials. In kiwifruit, AcSVP2 affects the duration of dormancy in cultivars that require high chill for dormancy release. In this study, we used a low-chill kiwifruit Actinidia chinensis 'Hort16A' to further study the function and regulation of AcSVP2. Overexpression of AcSVP2 in transgenic A. chinensis delayed budbreak in spring. A reduction in the active trimethylation histone marks of the histone H3K4 and acetylation of histone H3 contributed to the reduction of AcSVP2 expression towards dormancy release, while the inactive histone marks of trimethylation of the histone H3K27 and H3K9 in AcSVP2 locus did not show significant enrichment at the end of winter dormancy. Analysis of expression in shoot buds showed that AcSVP2 transcript was elevated in dormant buds during winter months and declined prior to budbreak, which was coordinated with expression of some of kiwifruit SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like genes. Screening of 101 transcription factors in an assay with a 2.3 kb promoter region of AcSVP2 found that kiwifruit SOC1-like genes are able to activate the AcSVP2 promoter. We further identified additional transcription factors associated with drought/osmotic stress and dormancy which may regulate AcSVP2 expression.

Time-to-event analysis to evaluate dormancy status of single-bud cuttings: an example for grapevines.

BACKGROUND: The reduced growth of plants during the winter causes a lack in the perceptibility of the phenological events making challenging the study of dormancy. For deciduous crops, dormancy is generally determined by evaluating budbreak of single-node cuttings that are exposed to conditions suitable for growth. However, the absence of a statistical basis for analyzing and interpreting the budbreak behavior evaluated as the percent budbreak, the average time to budbreak and the time to reach 50% budbreak, has caused inconsistent and contradictory criteria to identify the dormancy status of different deciduous crops. RESULTS: In this study, a method was developed to analyze the duration between sampling and budbreak of single-node cuttings and to estimate the dormancy status for grapevines (Vitis vinifera L.) based on the time-to-event distribution of the observations. This method estimates the probability curve of budbreak for each sample and classifies each curve into paradormancy, endodormancy, and ecodormancy according to the significance when compared to a sample curve estimated from cuttings collected during paradormancy and referred to as "reference." CONCLUSION: The approach described in this study provided a comparison of the budbreak distribution of cuttings collected during distinct phases with a confidence of 95%. It also allowed the estimation of the date of occurrence of the dormancy stages for two grapevine cultivars 'Cabernet Sauvignon' and 'Chardonnay,' based on the variability within the sampling season rather than on fixed arbitrary criteria. This approach can also be used to analyze budbreak data of single-node cuttings from other common deciduous crops.

High-resolution genetic linkage map of European pear (Pyrus communis) and QTL fine-mapping of vegetative budbreak time.

BACKGROUND: Genomic analysis technologies can promote efficient fruit tree breeding. Genotyping by sequencing (GBS) enables generating efficient data for high-quality genetic map construction and QTL analysis in a relatively accessible way. Furthermore, High-resolution genetic map construction and accurate QTL detection can significantly narrow down the putative candidate genes associated with important plant traits. RESULTS: We genotyped 162 offspring in the F1 'Spadona' x 'Harrow Sweet' pear population using GBS. An additional 21 pear accessions, including the F1 population's parents, from our germplasm collection were subjected to GBS to examine diverse genetic backgrounds that are associated to agriculturally relevant traits and to enhance the power of SNP calling. A standard SNP calling pipeline identified 206,971 SNPs with Asian pear ('Suli') as the reference genome and 148,622 SNPs with the European genome ('Bartlett'). These results enabled constructing a genetic map, after further stringent SNP filtering, consisting of 2036 markers on 17 linkage groups with a length of 1433 cM and an average marker interval of 0.7 cM. We aligned 1030 scaffolds covering a total size of 165.5 Mbp (29%) of the European pear genome to the 17 linkage groups. For high-resolution QTL analysis covering the whole genome, we used phenotyping for vegetative budbreak time in the F1 population. New QTLs associated to vegetative budbreak time were detected on linkage groups 5, 13 and 15. A major QTL on linkage group 8 and an additional QTL on linkage group 9 were confirmed. Due to the significant genotype-by-environment (GxE) effect, we were able to identify novel interaction QTLs on linkage groups 5, 8, 9 and 17. Phenotype-genotype association analysis in the pear accessions for main genotype effect was conducted to support the QTLs detected in the F1 population. Significant markers were detected on every linkage group to which main genotype effect QTLs were mapped. CONCLUSIONS: This is the first vegetative budbreak study of European pear that makes use of high-resolution genetic mapping. These results provide tools for marker-assisted selection and accurate QTL analysis in pear, and specifically at vegetative budbreak, considering the significant GxE and phenotype-plasticity effects.