Feeding limestone buffer to limit-fed dairy steers fed a high inclusion rate of distiller grains.

The study objective was to evaluate dietary limestone buffer inclusion rates for impacting nutrient digestibility and excretion when growing dairy cattle are fed high distiller grains with solubles (DDGS) limit-fed ration. The hypothesis was that feeding more limestone buffer would offset a low rumen pH when feeding a high DDGS inclusion rate. Five ruminally cannulated Holstein and Brown Swiss steers were used in a crossover design having 2-wk periods to evaluate high and low buffer inclusion rates when limit-fed. Treatments were similar in ingredient composition being: 1) 40% DDGS at 0.80% high CaCO3 buffer inclusion (HIGH) and 2) 40% DDGS with 0.25% low CaCO3 buffer inclusion (LOW) with the remaining ration consisting of grass hay with minerals and vitamins. Rations were limit-fed at 2.50% of body weight (BW) using Calan feeding doors with steers being weighed every 2 wk with ration amounts adjusted accordingly. Rumen fluid was collected via the ruminal cannula at the start and end of each period for pH, ammonia-n, and volatile fatty acid (VFA)s. Fecal grab samples were collected at the end of each period for measurement of total-tract nutrient digestibility. There were no treatment-by-period interactions (P > 0.10) for any of the growth parameters measured. Gains in frame growth parameters were similar (P > 0.10) for steers fed both rations. BWs and gains were similar (P > 0.10) for steers fed both rations. Steers fed the LOW ration demonstrated a numeric improvement in average daily gain combined with a tendency (P < 0.06) for lower dry matter intake resulting in an improved (P < 0.01) feed efficiency (gain:feed) compared with steers fed the HIGH ration. Steers fed HIGH demonstrated greater (P < 0.01) ruminal ammonia-N and isovalerate concentrations compared with steers fed the LOW buffer ration. Steers fed both buffer inclusion rates were similar (P > 0.10) for ruminal pH and remaining VFAs concentrations. Steers fed both buffer inclusion rates were similar (P > 0.10) in DM and organic matter digestibilities. Limit-feeding a high DDGS inclusion rate ratio combined with a low buffer inclusion rate improved feed efficiency while maintaining growth performance. The study hypothesis was rejected in that feed efficiency can be enhanced when feeding a high DDGS ration by feeding a low calcium carbonate buffer.

Electricity production and nutrient recovery from waste activated sludge via microbial fuel cell and subsequent struvite crystallization: Effect of low temperature thermo-alkaline pretreatment.

Microbial fuel cell (MFC) and subsequent struvite crystallization are available low-carbon environmental- friendly techniques for resource utilization of waste activated sludge (WAS). In this study, low temperature thermo-alkaline pretreatment (LTTAP) was innovatively proposed for enhancing MFC electricity generation and subsequent struvite crystallization from WAS. The results indicated that LTTAP at 75 °C and pH 10 not only substantially shortened the start-up time of MFC to 3-4 days, but also significantly increased maximum power density to 5.38 W/m3. Moreover, thermo-alkaline pretreated WAS effectively exhibited stable and high output voltage over long period, compared to unpretreated WAS. Furthermore pretreated WAS can provide an effective pH buffering function for MFC operation. In addition, about 90 % of phosphate in the pretreated WAS supernatant was recovered by struvite crystallization. The findings herein provided a new route for enhancing electricity production and nutrient recovery from WAS, which can realize the full-scale applicationof WAS resource utilization.

Desalting strategies for native mass spectrometry.

In native mass spectrometry (MS) salts are indispensable for preserving the native structures of biomolecules, but detrimental to mass sensitivity, resolution, and accuracy. Such a conflict makes desalting in native MS more challenging, distinctive, and sample-dependent than in peptide-centric MS. This review first briefly introduces the charged residue mechanism whereby native-like gaseous protein ions are released from electrospray droplets, revealing a higher degree of salt adduction than denatured proteins. Subsequently, this review summarizes and explores the existing strategies, underlying mechanisms and future perspectives of desalting in native MS. These strategies mainly focus on buffer exchange into volatile salts (offline and online approaches), addition of solution additives (e.g., anion, supercharging reagent, solution phase chelator and amino acid), use of submicron electrospray emitters (down to 60 nm), and other potential approaches (e.g., induced and electrophoretic nanoelectrospray ionization). The strategies of online buffer exchange and using nanoscale electrospray emitters are highlighted. This review would not only be a valuable addition to the field of sample preparation in MS, but would also serve as a beginner's guide to desalting in native MS.

Alternative buffer systems in biopharmaceutical formulations and their effect on protein stability.

The formulation of biopharmaceutical drugs is designed to eliminate chemical instabilities, increase conformational and colloidal stability of proteins, and optimize interfacial stability. Among the various excipients involved, buffer composition plays a pivotal role. However, conventional buffers like histidine and phosphate buffers may not always be the optimal choice for all monoclonal antibodies (mAbs). In this study, we investigated the effects of several alternative buffer systems on seven different mAbs, exploring various combinations of ionic strengths, concentrations of the main buffer component, mAb concentrations, and stress conditions. Protein stability was assessed by analyzing soluble aggregate formation through size exclusion chromatography. At low protein concentrations, protein instability after temperature stress was exclusively observed in the bis-TRIS/ glucuronate buffer. Conversely, freeze-thaw stress led to a significant increase in aggregate formation in tested formulations, highlighting the efficacy of several alternative buffers, particularly arginine/ citrate, in preserving protein stability. Under temperature stress, the introduction of arginine to histidine buffer systems provided additional stabilization, while the addition of lysine resulted in protein destabilization. Similarly, the incorporation of arginine into histi-dine/HCl buffer further enhanced protein stability during freeze--thaw cycles. At high protein concentrations, the histidine/citrate buffer emerged as one of the most optimal choices for addressing temperature and light-induced stress. The efficacy of histidine buffers in combating light stress might be attributed to the light-absorbing properties of histidine molecules. Our findings demonstrate that the development of biopharmaceutical formulations should not be confined to conventional buffer systems, as numerous alternative options exhibit comparable or even superior performance.

Electrochemical sensing of vitamin B6 (pyridoxine) by adapted carbon paste electrode.

The recent investigation targets to use adapted carbon paste (CP) with copper nanoparticles (CuNs) operating in a phosphate buffer (PBS) medium with a pH range of 5.0-8.0, to synthesize a novel, susceptible, and simple electrochemical sensor for the detection of one of the most important drugs, vitamin B6. Copper (Cu) is one of the most three common essential trace elements found in the bodies of both humans and animals, along with iron and zinc for all crucial physiological and biochemical functions. Its properties, which are assessed using a variety of methods including scanning electron microscopy (SEM), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS), have also drawn a lot of attention recently. We considered the effects of pH, buffer, scan rate, interference, and calibration curve. The susceptible electrode's linear calibration curve encompassed concentration values between 8.88 and 1000.0 µM. The calculated limits of detection and quantification were 32.12 and 107.0 µM, respectively. Furthermore, this method was established in real human urine samples and drug validation which have been shown satisfactory results for vitamin B6 detection.

Efficient one step extraction process of Gramicidin S from Aneurinibacillus aneurinilyticus biomass.

Currently, Gramicidin S (GR-S) is produced enzymatically with the drawback of the presence of trifluoroacetic acid (TFA) or produced by fermentation involving several separation and purification steps. Therefore, this study is focused on the use of green solvents as unique extraction step to produce Gramicidin S from microbial biomass of Aneurinibacillus aneurinilyticus. Among the tested solvents, such as ethanol, acidic ethanol or buffer phosphate, the most favorable was acidic ethanol, extracting 96% of Gramicidin S from cells with a purity of 90%. Using acidic ethanol, extraction time within the range of 30-120 min exhibited minimal impact on Gramicidin S yield, whereas the biomass-to-extractant ratio emerged as a critical parameter. Gramicidin S extracts were characterized using Fourier Transform Infrared Spectroscopy (FTIR), Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS), and Electrospray Ionization Mass Spectrometry (ESI-MS) coupled with Ultra Performance Liquid Chromatography (UPLC) and compared with commercial Gramicidin S.

Interactions of human serum albumin with phosphate and Tris buffers: impact on paclitaxel binding and nanoparticles self-assembly.

AIM: To investigate the conformational changes in human serum albumin (HSA) caused by chemical (CD) and thermal denaturation (TD) at pH 7.4 and 9.9, crucial for designing controlled drug delivery systems with paclitaxel (PTX). METHODS: Experimental and computational methods, including differential scanning calorimetry (DSC), UV-Vis and intrinsic fluorescence spectroscopy, mean diameter, polydispersity index (PDI), ζ-potential, encapsulation efficiency (EE), in vitro release and protein docking studies were conducted to study the HSA denaturation and nanoparticles (NPs) preparation. RESULTS: TD at pH 7.4 produced smaller NPs (287.1 ± 12.9 nm) than CD at pH 7.4 with NPs (584.2 ± 47.7 nm). TD at pH 9.9 exhibited high EE (97.3 ± 0.2%w/w) with rapid PTX release (50% within 1h), whereas at pH 7.4 (96.4 ± 2.1%w/w), release only 40%. ζ-potentials were around -30 mV. CONCLUSION: Buffer type and pH significantly influence NP properties. TD in PBS at pH 7.4, provided optimal conditions for a stable and efficient drug delivery system.

Slot Blot- and Electrospray Ionization-Mass Spectrometry/Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry-Based Novel Analysis Methods for the Identification and Quantification of Advanced Glycation End-Products in the Urine.

Proteins, saccharides, and low molecular organic compounds in the blood, urine, and saliva could potentially serve as biomarkers for diseases related to diet, lifestyle, and the use of illegal drugs. Lifestyle-related diseases (LSRDs) such as diabetes mellitus (DM), non-alcoholic steatohepatitis, cardiovascular disease, hypertension, kidney disease, and osteoporosis could develop into life-threatening conditions. Therefore, there is an urgent need to develop biomarkers for their early diagnosis. Advanced glycation end-products (AGEs) are associated with LSRDs and may induce/promote LSRDs. The presence of AGEs in body fluids could represent a biomarker of LSRDs. Urine samples could potentially be used for detecting AGEs, as urine collection is convenient and non-invasive. However, the detection and identification of AGE-modified proteins in the urine could be challenging, as their concentrations in the urine might be extremely low. To address this issue, we propose a new analytical approach. This strategy employs a method previously introduced by us, which combines slot blotting, our unique lysis buffer named Takata's lysis buffer, and a polyvinylidene difluoride membrane, in conjunction with electrospray ionization-mass spectrometry (ESI)/matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). This novel strategy could be used to detect AGE-modified proteins, AGE-modified peptides, and free-type AGEs in urine samples.

Buffer system improves the removal of host cell protein impurities in monoclonal antibody purification.

Polysorbates (PS) are commonly used as stabilizers of biopharmaceuticals such as monoclonal antibodies (mAbs). However, they are prone to chemical and enzymatic degradation. The latter can be caused by residual host cell proteins (HCPs) in the drug substance. Degradation affects the functionality of the PS surfactant which can lead to formation of particles. An increasing number of publications describe enzymatic PS degradation. Significant efforts have been made to characterize HCP removal during Downstream Processing (DSP) of mAbs and to develop mitigation strategies. Here we describe the use of glycine buffer for acidic elution in Protein A affinity chromatography compared to acetate buffer, which is more commonly used in the biopharmaceutical industry. Increased turbidity was observed during pH re-adjustment after low pH virus inactivation when using glycine buffer. Analytical data suggests that this turbidity is caused by the formation of precipitates which include HCP and DNA impurities. Additionally, as a zwitterion, glycine does not contribute to conductivity; this further enhances HCP removal during anion-exchange flow-through chromatography. Although glycine is well known as a possible elution buffer for Protein A affinity chromatography, its positive impact on HCP removal and PS stability have not yet been described in literature.

Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes.

BACKGROUND: Vibrio natriegens, a halophilic marine γ-proteobacterium, holds immense biotechnological potential due to its remarkably short generation time of under ten minutes. However, the highest growth rates have been primarily observed on complex media, which often suffer from batch-to-batch variability affecting process stability and performance. Consistent bioprocesses necessitate the use of chemically defined media, which are usually optimized for fermenters with pH and dissolved oxygen tension (DOT) regulation, both of which are not applied during early-stage cultivations in shake flasks or microtiter plates. Existing studies on V. natriegens' growth on mineral media report partially conflicting results, and a comprehensive study examining the combined effects of pH buffering, sodium concentration, and medium osmolality is lacking. RESULTS: This study evaluates the influence of sodium concentration, pH buffering, and medium osmolality on the growth of V. natriegens under unregulated small-scale conditions. The maximum growth rate, time of glucose depletion, as well as the onset of stationary phase were observed through online-monitoring the oxygen transfer rate. The results revealed optimal growth conditions at an initial pH of 8.0 with a minimum of 300 mM MOPS buffer for media containing 20 g/L glucose or 180 mM MOPS for media with 10 g/L glucose. Optimal sodium chloride supplementation was found to be between 7.5 and 15 g/L, lower than previously reported ranges. This is advantageous for reducing industrial corrosion issues. Additionally, an osmolality range of 1 to 1.6 Osmol/kg was determined to be optimal for growth. Under these optimized conditions, V. natriegens achieved a growth rate of 1.97 ± 0.13 1/h over a period of 1 h at 37 °C, the highest reported rate for this organism on a mineral medium. CONCLUSION: This study provides guidelines for cultivating V. natriegens in early-stage laboratory settings without pH and DOT regulation. The findings suggest a lower optimal sodium chloride range than previously reported and establish an osmolality window for optimal growth, thereby advancing the understanding of V. natriegens' physiology. In addition, this study offers a foundation for future research into the effects of different ions and carbon sources on V. natriegens.

Optimized method for higher yield of alveolar macrophage isolation for ex vivo studies.

Alveolar macrophages (AMs) are a fully differentiated lung-resident immune cell population and are a critical component of lung immunity. AMs can be easily isolated from mice via bronchoalveolar lavage fluid (BALF) collection. The quality and quantity of AMs in BALF isolation are critical for generating reliable and high-quality data for ex vivo studies. Traditional techniques use ice-cold (4°C) buffer to collect AMs in BALF and result in low yield. Hence, a new method that consistently gives a higher yield of AMs is needed. We demonstrate here an optimized method that significantly increases the quantity of AM recovery in BALF (>2.8 times than the traditional method). Our method uses a warm-buffer (37°C) containing EDTA. We compared the viability and functional parameters (cytokine/chemokine expression, phagocytosis) of AMs isolated by our new and traditional methods. Our study revealed that AMs collected using our method have similar viability and functional characteristics to those collected using traditional method. Hence, our new method can be used for the collection of a higher number of AMs without altering their function. This protocol might also be useful for isolating tissue-resident immune cells from other anatomical sites for ex vivo and other downstream applications.

Efficacy of Carbonate Buffer Mixture in Preventing Hoof Lamella Injury Associated with Subacute Ruminal Acidosis in Dairy Goats.

Subacute ruminal acidosis (SARA) is a prevalent metabolic disorder in highly productive dairy cows that results in serious issues, including hoof lamellar injuries. This study aimed to investigate the efficacy of a carbonate buffer mixture (CBM) in preventing hoof lamella injury in dairy goats, a species also susceptible to SARA due to similar feeding practices over a 17-week period. Twenty-four healthy dairy goats were randomly assigned to three groups: control, SARA, and CBM groups. The control group received a standardized diet, whereas the SARA and CBM groups were subjected to a high-grain feeding regimen to induce SARA. The CBM group received a daily supplement of 10 g CBM mixed with their diet. Clinical assessments, including body temperature, rumen pH, inflammatory markers, matrix metalloproteinases (MMPs), and hoof lamellar injuries, were monitored throughout the study. The results showed that the CBM group maintained a more stable rumen pH and had lower levels of inflammatory markers than the SARA group did. The incidence of hoof lamellar injury was slightly lower in the CBM group. These findings suggest that long-term CBM supplementation may mitigate SARA-associated hoof lamella injury in dairy goats by regulating the rumen environment, fostering the growth of healthy bacterial communities, and by reducing the production of harmful metabolites. The use of CBM as a dietary supplement may have significant implications in improving the health, welfare, and productivity of dairy animals.

Bidirectional pH Buffer Effect Facilitates High-Reversible Aqueous Zinc Ion Batteries.

Aqueous zinc ion batteries (AZIBs) stand out from the crowd of energy storage equipment for their superior energy density, enhanced safety features, and affordability. However, the notorious side reaction in the zinc anode and the dissolution of the cathode materials led to poor cycling stability has hindered their further development. Herein, ammonium salicylate (AS) is a bidirectional electrolyte additive to promote prolonged stable cycles in AZIBs. NH4 + and C6H4OHCOO- collaboratively stabilize the pH at the interface of the electrolyte/electrode and guide the homogeneous deposition of Zn2+ at the zinc anode. The higher adsorption energy of NH4 + compared to H2O on the Zn (002) crystal plane mitigates the side reactions on the anode surface. Moreover, NH4 + is similarly adsorbed on the cathode surface, maintaining the stability of the electrode. C6H4OHCOO- and Zn2+ are co-intercalation/deintercalation during the cycling process, contributing to the higher electrochemical performance of the full cell. As a result, with the presence of AS additive, the Zn//Zn symmetric cells achieved 700 h of highly reversible cycling at 5 mA cm-2. In addition, the assembled NH4V4O10(NVO)//Zn coin and pouch batteries achieved higher capacity and higher cycle lifetime, demonstrating the practicality of the AS electrolyte additive.

Inactivation Validation of Ebola, Marburg, and Lassa Viruses in AVL and Ethanol-Treated Viral Cultures.

High-consequence pathogens such as the Ebola, Marburg, and Lassa viruses are handled in maximum-containment biosafety level 4 (BSL-4) laboratories. Genetic material is often isolated from such viruses and subsequently removed from BSL-4 laboratories for a multitude of downstream analyses using readily accessible technologies and equipment available at lower-biosafety level laboratories. However, it is essential to ensure that these materials are free of viable viruses before removal from BSL-4 laboratories to guarantee sample safety. This study details the in-house procedure used for validating the inactivation of Ebola, Marburg, and Lassa virus cultures after incubation with AVL lysis buffer (Qiagen) and ethanol. This study's findings show that no viable virus was detectable when high-titer cultures of Ebola, Marburg, and Lassa viruses were incubated with AVL lysis buffer for 10 min, followed by an equal volume of 95% ethanol for 3 min, using a method with a sensitivity of ≤0.8 log10 TCID50 as the limit of detection.

Buffer Stock Inventory Control Mechanism: An Approach of Minimizing the Buffer Stock Level Through Segmentation at a Tertiary Care Rural Hospital.

Background Efficient management of hospital inventories, particularly pharmaceuticals, is essential for ensuring timely patient care and optimizing resource allocation. Buffer stock is the backup stock, which is kept for providing a supply of drugs when the main stock is consumed and new stock is in the process of procurement. The buffer stock is sometimes kept in excess, which causes unnecessary overutilization of the financial resources. Therefore, the author's team aimed to optimize the inventory control of the buffer stock. This study addressed these challenges by integrating always better control (ABC) and vital, essential, and desirable (VED) analysis to categorize drugs based on consumption patterns and clinical importance to minimize buffer stock and ensure optimum resource allocation. To overcome these challenges, there is a need to integrate financial and clinical factors into inventory management decisions. Methods This study was done at the pharmacy of an apex hospital situated in a rural region of western India. The study aimed at the categorization of drugs that require strict inventory management control. The drugs and consumables used in the month of April 2024 in the hospital pharmacy were considered for the study & inventory control methods were applied.  Results The study discovered that a subgroup of drugs accounted for a significant portion of the pharmacy's budget, with 12.18% categorized as high-value (Category A), 22.07% as moderate-value (Category B), and 65.74% as low-value (Category C) items. ABC analysis showed that Category A drugs consumed 70.1% of the average daily expenditure (ADE) of the pharmacy, while Category B and Category C drugs contributed 19.9% and 9.98% of the ADE, respectively. VED analysis classified drugs into vital (11.34%), essential (58.26%), and desirable (30.39%) categories. The ABC-VED matrix further categorized drugs into subgroups, with class I items constituting 20.62% of the inventory and accounting for 73.82% of the ADE. Class II items comprised 56.69% of the inventory, consuming 22.91% of the ADE, while class III items constituted 22.68% of the inventory and consumed 3.28% of the ADE. Conclusion ABC and VED analysis integration provides a comprehensive framework for optimizing drug inventory management in healthcare facilities. By considering both financial and clinical factors, this approach enables tailored management strategies, minimizing buffer stock, eliminating stockouts, and enhancing patient care. The rural hospital's location is responsible for maintaining the buffer stock level and the reorder level quantity. This study highlighted the role of inventory control techniques in healthcare facilities, particularly in pharmacies, to ensure the availability of essential medications while optimizing resource utilization. By integrating segmentation techniques such as ABC and VED analysis, this study provided valuable insights into categorizing drugs based on their consumption patterns, criticality, and importance in the clinical area.

Effect of Fixed Orthodontic Appliances on Oral Microbial Changes and Dental Caries Risk in Children: A 6-Month Prospective Study.

Introduction: Orthodontic treatment can alter the oral environment, leading to plaque accumulation and changes in microbial colonization. This study aimed to assess microbiological changes in 10 to 16-year-old children undergoing fixed orthodontic treatment. Methodology: The study included children aged 10-16 years, with one group wearing fixed orthodontic appliances and a control group without brackets. Data on salivary pH, buffer capacity, Streptococcus mutans, and Lactobacillus spp were collected at baseline and after 6 months. Logistic regression models evaluated associations between orthodontic treatment and elevated bacterial levels, adjusting for age, gender, pH, and buffer capacity. Results: Wearing fixed orthodontic appliances was significantly associated with elevated Streptococcus mutans (aOR: 6.62, 95% CI: 1.91-21.88) and Lactobacillus spp (aOR: 9.49, 95% CI: 2.57-35.07). Gender showed a significant association with Lactobacillus spp levels (aOR: 0.28, 95% CI: 0.09-0.87). Conclusion: Fixed orthodontic appliances significantly impact oral microbial changes, underscoring the need for proper oral hygiene during orthodontic treatment to reduce dental caries risk.

Highly effective strategy for isolation of mononuclear cells from frozen cord blood.

BACKGROUND AIMS: Cord blood mononuclear cells (CBMCs) comprise a variety of single-nucleated cells found in the cord blood, mainly consisting of monocytes and lymphocytes. They also include a smaller proportion of other cell types, such as hematopoietic stem and progenitor cells (HSPCs) and mesenchymal stromal cells (MSCs). CBMCs are vital for acquiring hematopoietic stem and progenitor cells, MSCs, and other immune cells, like natural killer cells. These cells are essential for supporting subsequent research and clinical applications. Although automated equipment for CBMC enrichment has shown promise, the high cost of these machines and the expense of disposable consumables limit their routine use. Furthermore, limited information is available on manual strategies for isolating CBMCs from cryopreserved cord blood. Therefore, we aimed to optimize the dilution buffer and refine the isolation procedure for CBMCs. METHODS: We enhanced the CBMC recovery rate from cryopreserved cord blood using an optimized dilution buffer and a modified isolation procedure. RESULTS: We achieved average recovery rates of 42.4 % and 54.3 % for CBMCs and CD34+ cells, respectively. Notably, all reagents used in the isolation procedure were of GMP-grade or pharmaceutical preparations, underscoring the potential clinical benefits of our strategy. DISCUSSION: We devised an optimized protocol suitable for routine research and clinical applications for enhanced recovery of CBMCs from cryopreserved cord blood units using an optimized dilution buffer and a modified isolation procedure.

Buffered versus Non-buffered Local Anaesthesia in Minor Oral Surgery - A Comparative Study.

Introduction: Commercially available local anaesthetics are acidic solutions associated with the vasopressor sting on injection, relatively slower onset of action and pain during palatal injections. The above drawbacks can be addressed by anaesthetic buffering. This prospective study was aimed at comparing the efficacy of buffered and non-buffered local anaesthesia in the extraction of grossly decayed maxillary molar teeth in relation to pain on local infiltration, onset and duration of action of local anaesthesia. Materials and Methods: This is a prospective randomised controlled trial done on 100 patients who required bilateral extraction of maxillary molar teeth. In the study group, patients were given buffered local anaesthesia (which was prepared by mixing 2% lignocaine with 1:80,000 adrenaline and 8.4% sodium bicarbonate) before extraction. In the control group, non-buffered local anaesthesia (2% lignocaine with 1:80,000 adrenaline) was given before extraction. Results: Statistical data confirmed that buffering reduces pain on infiltration, decreases the onset and increases the duration of action of the local anaesthesia compared to non-buffered local anaesthesia. All the parameters measured were statistically significant (P = 0.001). Discussion: The study concludes that buffered local anaesthesia was more beneficial than non-buffered local anaesthesia in reducing pain on injection, providing a quicker onset of local anaesthesia and increasing the duration of action of the local anaesthesia. Buffering is a safe, easy and efficient process and should be routinely followed to provide a better experience to the patients.

The Buffer Capacity of Polyelectrolyte Microcapsules Depends on the Type of Template.

One of the key physicochemical parameters of polyelectrolyte microcapsules (PMCs) is their buffer capacity (BC). The BC of the microcapsules allows for an assessment of the change in protonation state across the entire polyelectrolyte system, which directly impacts the buffer barrier of PMCs, as well as the stability and physical properties of their shell. However, the buffer capacity of PMCs and their behavior under changes in ionic strength and temperature can differ depending on the type of core used to form the microcapsules. As part of this study, we revealed the buffer capacity (BC) of polyelectrolyte microcapsules formed on polystyrene cores (PMCPs) and studied the influence of ionic strength and environmental temperature on the BC of these capsules. We found that the buffer capacity of PMCPs differs from the BC of water at a pH above 8; the addition of sodium chloride leads to an increase in buffer capacity in alkaline conditions, and conversely, thermal treatment leads to its decrease at a pH of 9. The results obtained are different from the BC of polyelectrolyte microcapsules formed on CaCO3 cores, which suggests a difference in the physicochemical properties of these types of capsules. The buffer capacity of polyelectrolyte microcapsules depends on the type of template used.

Evaluation and Development of Analytical Procedures to Assess Buffering Capacity of Carbonate Ruminant Feed Buffers.

The inclusion of rumen buffers in ruminant feeds has gained widespread adoption for the prevention of rumen acidosis, thereby avoiding the negative production and health consequences of low rumen pH and resulting in improved feed efficiency. Benchmarking and quality controlling the performance of rumen buffer materials is of significant interest to feed mills and end-user producers. The aim of this study was to evaluate, develop and optimise a laboratory protocol to consistently and robustly evaluate rumen buffering materials in order to predict their in vivo efficacy. Three different methods were evaluated for determining the buffering potential of carbonate buffer materials: (a) 2 and 8 h static pH, (b) 8 h fixed HCl acid load addition and (c) 3 h acidotic diet simulation using acetic acid. Buffer material, threshold pH, test duration and interactions between all three variables were significant (p < 0.001) in evaluating the performance of the buffer materials. The acidotic diet simulation was found to provide a different ranking of materials to the 8 h fixed HCl acid load methodology. The results highlight the importance of method selection and test parameters for accurately evaluating the potential efficacy of rumen buffer materials.