ABSTRACT
Los tumores de células granulosas afectan raramente al esófago. Presentamos el caso de tumores de células granulosas del esófago y revisar la literatura. Se presenta una paciente de 21 años de edad y piel negra con síntomas de reflujo gastroesofágico. La endoscopia digestiva demostró un tumor de 2 cm que protruía hacia la luz del órgano, localizado en el tercio inferior, con mucosa sana. El esofagograma corroboró el hallazgo en la porción abdominal del órgano y la biopsia endoscópica informó que se trataba de tumores de células granulosas. El acceso videolaparoscópico fue convertido a cirugía convencional debido a la abertura mucosa y a que quedaba un pequeño fragmento del tumor. El periodo posoperatorio transcurrió sin complicaciones. Un año después de la operación no existía evidencia de recidiva. Los tumores de células granulosas son raros en el esófago. Los tumores pequeños (< 1 cm) pueden ser tratados conservadoramente, mientras que los de mayor volumen deben ser resecados mediante diferentes técnicas: endoscópicas y cirugía de mínimo acceso o convencional(AU)
Granulosa cell tumors rarely affect the esophagus. We present the case of granulosa cell tumors of the esophagus and literature review. A 21-year-old patient of black skin presents with symptoms of gastroesophageal reflux. The digestive endoscopy showed a 2-cm tumor that protruded towards the organ lumen, located in the lower third, with healthy mucosa. The esophagogram corroborated the finding in the abdominal portion of the organ, and the endoscopic biopsy reported that there were granulosa cell tumors. Videolaparoscopic access was converted to conventional surgery due to the mucosal opening and because a small fragment of the tumor was remaining. The postoperative period had no complications. One year after the operation, there was no evidence of recurrence. Granulosa cell tumors are rare in the esophagus. Small tumors (smaller than 1 cm) can be treated conservatively, while larger tumors should be resected using different techniques: endoscopic and minimal access or conventional surgery(AU)
Subject(s)
Humans , Female , Adult , Esophagoscopy/methods , Granular Cell Tumor/diagnostic imaging , Endoscopy/methodsABSTRACT
Los tumores de células granulosas afectan raramente al esófago. Presentamos el caso de tumores de células granulosas del esófago y revisar la literatura. Se presenta una paciente de 21 años de edad y piel negra con síntomas de reflujo gastroesofágico. La endoscopia digestiva demostró un tumor de 2 cm que protruía hacia la luz del órgano, localizado en el tercio inferior, con mucosa sana. El esofagograma corroboró el hallazgo en la porción abdominal del órgano y la biopsia endoscópica informó que se trataba de tumores de células granulosas. El acceso videolaparoscópico fue convertido a cirugía convencional debido a la abertura mucosa y a que quedaba un pequeño fragmento del tumor. El periodo posoperatorio transcurrió sin complicaciones. Un año después de la operación no existía evidencia de recidiva. Los tumores de células granulosas son raros en el esófago. Los tumores pequeños (< 1 cm) pueden ser tratados conservadoramente, mientras que los de mayor volumen deben ser resecados mediante diferentes técnicas: endoscópicas y cirugía de mínimo acceso o convencional(AU)
Granulosa cell tumors rarely affect the esophagus. We present the case of granulosa cell tumors of the esophagus and literature review. A 21-year-old patient of black skin presents with symptoms of gastroesophageal reflux. The digestive endoscopy showed a 2-cm tumor that protruded towards the organ lumen, located in the lower third, with healthy mucosa. The esophagogram corroborated the finding in the abdominal portion of the organ, and the endoscopic biopsy reported that there were granulosa cell tumors. Videolaparoscopic access was converted to conventional surgery due to the mucosal opening and because a small fragment of the tumor was remaining. The postoperative period had no complications. One year after the operation, there was no evidence of recurrence. Granulosa cell tumors are rare in the esophagus. Small tumors (smaller than 1 cm) can be treated conservatively, while larger tumors should be resected using different techniques: endoscopic and minimal access or conventional surgery(AU)
Subject(s)
Humans , Female , Adult , Esophagoscopy/methods , Granular Cell Tumor , Endoscopy/methodsABSTRACT
O tumor de células granulares é uma lesão incomum que apresenta predileção pela cavidade oral, por apresentar baixa taxa de recidiva, o tratamento de escolha é a excisão cirúrgica simples. O objetivo caracterizar um caso de tumor de células granulares bifocal em mucosa jugal e explanar características clínicas e histopatológicas acerca da lesão. Relata-se o caso de uma paciente de 60 anos apresentando dois pequenos nódulos de superfície lisa em mucosa jugal, consistência fibroelástica e coloração levemente amarelada. Foi realizada a excisão cirúrgica das duas lesões a partir de uma biópsia excisional, onde microscopicamente foi observada uma neoplasia de células granulares arranjadas em ilhas, chegando ao diagnóstico de Tumor de células granulares. O diagnóstico final da lesão foi obtido a partir do exame histopatológico, visto que, a aparência clínica da lesão é inespecífica, sendo de extrema importância a realização da biópsia excisional. A paciente continua em acompanhamento há 6 meses e não demonstra recidiva da lesão(AU)
The granulosa cell tumor constitutes a rare disease that predominates in the tongue and has a low rate of recurrence, simply by surgical removal. The objective is to characterize a case of granulosa cells bifocal tumor in the oral mucosa with some clinical and histopathologic features to this injury. A 60-year-old patient presented with two small nodules in the smooth surface, consistency and slightly yellowish fibroelastic. These lesions were removed by excisional biopsy. From the microscopic point of view, it was demonstrated that there was a neoplasia with granule cells in some areas and the diagnosis was granular cell tumor. The definitive diagnosis of the lesion is obtained by histopathology, as the clinical appearance of the lesion is nonspecific, so it is very important to perform the excisional biopsy. The patient remains under follow-up for six months and shows no recurrence(AU)
El tumor de células granulosas es una enfermedad rara que predomina en la lengua y tiene una baja tasa de recurrencia; se trata mediante la extirpación quirúrgica simple. El próposito es caracterizar un caso de tumor de células granulosas bifocal en la mucosa bucal con algunas características clínicas y histopatológicas de esta lesión. Se presenta un paciente de 60 años con dos pequeños nódulos de superficie lisa, consistencia fibroelástica y ligeramente amarillento. Estas lesiones fueron extirpadas por biopsia excisional. Desde el punto de vista microscópico se demostró una neoplasia con células granulares en islas y se llegó al diagnóstico de tumor de células granulares. El diagnóstico definitivo de la lesión se obtiene de la histopatología, pues el aspecto clínico de la lesión es inespecífica, por lo que resulta muy importante la realización de la biopsia excisional. El paciente continúa bajo seguimiento durante 6 meses y no muestra la recurrencia(AU)
Subject(s)
Humans , Female , Middle Aged , Biopsy/methods , Granular Cell Tumor/diagnosis , Granular Cell Tumor/pathology , Mouth Mucosa/injuriesABSTRACT
The objective of this study was to evaluate the vitrification of bovine preantral follicles with dimethylsulfoxide (D) and sucrose (S) plus α-tocopherol 5mmol/L (T5) or 10mmol/L (T10) and, evaluate the thawed with minimal essential medium (m) with or without sucrose (s). Ovaries of cows were collected from slaughterhouse for the experiment I (n=66) and II (n=51). In the laboratory ovarian fragments were randomly assigned either to fresh control and 8 vitrification treatments (Controle and Dm; Dms, DSm; DSms; DST5m; DST5ms; DST10m; DST10ms). Ovarian fragments were placed in vitrification solution (5 min) and immersed in liquid nitrogen (-196°C), after a week, the fragments were thawed and analyzed. In the experiments I, preantral follicles were morphologically observed for histological evaluation, (normal; degenerated and developing of stage). In the experiment II, preantral follicles were mechanically isolated from ovarian tissue and examined with trypan blue, where dead and live corresponded to stained or non-stained. The treatments DSm, DSms and DST10m were effective in preserving the morphology in situ. However, the viability of isolated preantral follicles after vitrification remained high only in treatment DST10m. Thus, DST10m preserves survival rates and morphological integrity during vitrification of bovine preantral follicles.
Os objetivos deste estudo foram avaliar a vitrificação de folículos pré-antrais bovinos com dimetilsulfóxido (D) e sacarose (S) adicionando α-tocoferol 5mmol/L (T5) ou 10mmol/L (T10) e, avaliar o aquecimento com meio essencial mínimo (m) com ou sem sacarose (s). Ovários de fêmeas bovinas foram coletados de abatedouro, para o experimento I (n= 66) e II (n= 51). No laboratório fragmentos ovarianos foram distribuídos aleatoriamente para o controle fresco e 8 tratamentos de vitrificação (Controle e Dm; Dms, a DSm; DSms; DST5m; DST5ms; DST10m; DST10ms). Os fragmentos ovarianos foram colocados na solução de vitrificação (5 min) e imersos em nitrogênio líquido (-196°C). Após uma semana os fragmentos foram aquecidos e analisados. No experimento I, folículos pré-antrais foram observados morfologicamente para avaliação histológica (normal, degenerados e estádio de desenvolvimento). No experimento II, folículos pré-antrais foram mecanicamente isolados do tecido ovariano e examinados com o azul de trypan, observando mortos e vivos corados e não corados respetivamente. Os tratamentos a DSm, DSms e DST10m foram eficazes na preservação da morfologia in situ. No entanto, a viabilidade de folículos pré-antrais isolados após a vitrificação manteve-se elevada apenas no tratamento DST10m. Assim, DST10m preservou as taxas de sobrevivência e integridade morfológica durante a vitrificação de folículos pré-antrais bovinos.
Subject(s)
Animals , Female , Cattle , alpha-Tocopherol , Dimethyl Sulfoxide , Ovarian Follicle/anatomy & histology , Oocytes , Sucrose , Vitrification , Antioxidants , Cryopreservation/veterinary , Granulosa CellsABSTRACT
El tumor de células granulosas (TCG) es una entidad infrecuente de la piel y tejidos blandos. Ocurre con más frecuencia en mujeres de raza negra, entre la segunda y sexta década de vida. La localización habitual es la cavidad oral, pero pueden aparecer en otros sitios. El compromiso vulvar ha sido reportado en el 5-16%. Aunque la mayoría son benignos, existe una rara variante maligna agresiva. El tratamiento de elección es la escisión quirúrgica amplia. Presentamos un caso de TCG en la vulva de una mujer de 65 años de edad y revisamos la literatura.
Granulosa cell tumor (GCT) is an uncommon skin and soft tissues disease. It occurs most often in black women between secondand sixth decade of life. Usual location involves oral cavity, but may appear in other sites. Vulvar commitment has been reportedin 5-16% of the cases. Mostly benign, but there is a rare aggressive malignant variant. Treatment choice is wide surgical excision.We report a 65-year-old woman with GCT of the vulva and review the literature.
