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Mem. Inst. Oswaldo Cruz ; 104(4): 649-654, July 2009. ilus, graf, tab
Article in English | LILACS | ID: lil-523735

ABSTRACT

Candida glabrata is an opportunistic fungal pathogen that can cause severe invasive infections and can evade phagocytic cell clearance. We are interested in understanding the virulence of this fungal pathogen, in particular its oxidative stress response. Here we investigated C. glabrata, Saccharomyces cerevisiae and Candida albicans responses to two different oxidants: menadione and cumene hydroperoxide (CHP). In log-phase, in the presence of menadione, C. glabrata requires Cta1p (catalase), while in a stationary phase (SP), Cta1p is dispensable. In addition, C. glabrata is less resistant to menadione than C. albicans in SP. The S. cerevisiae laboratory reference strain is less resistant to menadione than C. glabrata and C. albicans; however S. cerevisiaeclinical isolates (CIs) are more resistant than the lab reference strain. Furthermore, S. cerevisiae CIs showed an increased catalase activity. Interestingly, in SP C. glabrata and S. cerevisiae are more resistant to CHP than C. albicans and Cta1p plays no apparent role in detoxifying this oxidant.


Subject(s)
Benzene Derivatives/pharmacology , Candida/drug effects , Oxidants/pharmacology , Oxidative Stress/drug effects , Saccharomyces cerevisiae/drug effects , /pharmacology , Candida albicans/drug effects , Candida albicans/metabolism , Candida glabrata/drug effects , Candida glabrata/metabolism , Candida/metabolism , Catalase/drug effects , Catalase/metabolism , Saccharomyces cerevisiae/metabolism
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