Relationship of selected properties of Cambisols to altitude and forest ecosystems of four vegetation grades.

Currently, little is known about the spatial variability of significant soil properties and their relationships to forest ecosystems of different vegetation grades. This work evaluates the variability of the properties of the upper layer of Cambisol taxa and their relationship to altitude and forest ecosystems of 2nd to 5th forest vegetation grades selected in the Western Carpathians using PCA and regression analysis. The content of clay, total carbon and total nitrogen, humus, energy, and ash in the soils varied between 5.43 and 11.53 %, 21-65 mg g-1, 1.9-4.7 mg g-1, 36-112 mg g-1, 438.4-5845.7 J g-1 and 852.9-946.3 mg g-1, and C/N, pHH2O, and pHKCl values ranged between 11.2 and 16.7, 4.0-5.8 and 3.1-4.6. PCA showed that EAC in the 3rd oak-beech vegetation grade had significantly higher pH values and significantly lower energy content, ESC in the 4th beech vegetation grade had a significantly higher ash content and a significantly lower energy content, and DC in the 5th fir-beech vegetation grade had a significantly higher content of Ct, Nt, and humus. Linear regression revealed a strong negative correlation between the energy content and soil reaction (R2 for pHH2O = 0.48; R2 for pHKCl = 0.38) for all Cambisol taxa. Ct content and ash show a strong negative correlation (R2 = 0.78). The positive relationship between altitude and FVGs was found only for the soil Ct (R2 = 0.87), Nt (R2 = 0.81), and humus content (R2 = 0.87). A strong negative linear relationship between altitude and FVGs showed the ash content (R2 = 0.77). In turn, the oscillatory, polynomial course had a relationship between the clay content (R2 = 0.65) and energy (R2 = 0.75) to altitude and FVGs. Recognizing significant soil variables and better understanding their impact on the development of forest ecosystems is a prerequisite for distinguishing areas with the highest risk of their damage under conditions of various anthropogenic interventions and climate change. Therefore, this topic continues to require increased research efforts. For this reason, a better understanding of the relationships between soil properties and ecologically differentiated communities of forest ecosystems will allow us to identify areas with the highest risk of ecological changes that could lead to the degradation of European forests in the future.

Long-term evolution of shrub prescribed burning effects on topsoil organic matter and biological activity in the Central Pyrenees (NE-Spain).

Since the last half of the 20th Century, scrubs have been invading subclimatic grasslands in the montane and subalpine stages of Spain due to the decrease of the grazing activity. This shrub encroachment reduces biodiversity and the ecopastoral value of the region and leads to the accumulation of woody fuel, which represents a high fire risk. To control the encroachment, prescribed burnings are performed but their effects on soils over the years are still undetermined. This study aims to research about the long-term effects of a prescribed burn of Echinospartum horridum (Vahl) Roth. on topsoil organic matter and biological activity. Soil sampling was carried out in Tella-Sin (Central Pyrenees, Aragón, Spain) and four treatments were selected: unburned (UB), immediately burned (B0), burned 6 years before (B6, mid-term) and burned 10 years before (B10, long-term). Among the obtained results, an immediately after burning decrease on ß-D-glucosidase activity (GLU) was found, which did not recover over time. Other properties did not have an immediate reduction but did so over time: total soil organic carbon (SOC), labile carbon (DOC), total nitrogen (TN), basal soil respiration (bSR). And others were not affected at all: microbial biomass carbon (MBC), and the microbial metabolic quotient (qCO2). Moreover, the normalized soil respiration (nSR) increased with the time, which implies an acceleration of the potential mineralization of soil organic carbon. In short, although the elimination of the dense shrubs by fire has not entailed major immediate soil modifications, which would be typical of a low severity prescribed burn, several mid- and long-term effects in the C cycle have been observed. Future studies will have to discern what is the main cause of these modifications (soil microbial composition, edaphoclimatic changes, lack of soil cover and soil loss, soil fertility, etc.).

Soil microbial communities following 20 years of fertilization and crop rotation practices in the Czech Republic.

BACKGROUND: Although fertilization and crop rotation practices are commonly used worldwide in agriculture to maximize crop yields, their long-term effect on the structures of soil microorganisms is still poorly understood. This study investigated the long-term impact of fertilization and crop rotation on soil microbial diversity and the microbial community structure in four different locations with three soil types. Since 1996, manure (MF; 330 kg N/ha), sewage sludge (SF; 330 and SF3x; 990 kg N/ha), and NPK (NPK; 330 kg N/ha) fertilizers were periodically applied to the soils classified as chernozem, luvisol and cambisol, which are among the most abundant or fertile soils used for agricultural purposes in the world. In these soils, potato (Solanum tuberosum L.), winter wheat (Triticum aestivum L.), and spring barley (Hordeum vulgare L.) were rotated every three years. RESULTS: Soil chemistry, which was significantly associated with location, fertilization, crop rotation, and the interaction of fertilization and location, was the dominant driver of soil microbial communities, both prokaryotic and fungal. A direct effect of long-term crop rotation and fertilization on the structure of their communities was confirmed, although there was no evidence of their influence on microbial diversity. Fungal and bacterial communities responded differently to fertilization treatments; prokaryotic communities were only significantly different from the control soil (CF) in soils treated with MF and SF3x, while fungal communities differed across all treatments. Indicator genera were identified for different treatments. These taxa were either specific for their decomposition activities or fungal plant pathogens. Sequential rotation of the three crops restricted the growth of several of the indicator plant pathogens. CONCLUSIONS: Long-term fertilization and crop rotation significantly altered microbial community structure in the soil. While fertilization affected soil microorganisms mainly through changes in nutrient profile, crop rotations lead to the attraction and repulsion of specific plant pathogens. Such changes in soil microbial communities need to be considered when planning soil management.

Short-term effects of land management change linked to cover crop on soil organic carbon in Mediterranean olive grove hillsides.

The spatial distribution of soil organic carbon (SOC) is essential to estimate the SOC reserves. Therefore, the soils ability to store organic carbon is a key factor for climate regulation and for other soil functions. The soil management and the topographic position play an important role in SOC variation, especially when the landscape is not uniform (Mediterranean areas). Many researches have explored the SOC distribution according to topographic position in hillsides for long-term, but very few studies have focused on the short term. Therefore, it is necessary to know, the changes that taking place in the soil due to land management change (LMC) in these irregular surfaces for sustainable agricultural production and its implications on climate change regulation. This study aims to assess the influence of topographic position and LMC on SOC stock (SOC-S) in Mediterranean olive groves (OG) soils in short term (2 years). In this line, three experimental plots were selected in three topographical position (summit - S, backslope - B and toeslope - T). In these plots, the land management was modified from conventional tillage (CT) to no tillage (NT) with application of pruned olive branch chippings branches and vegetation cover (spontaneous vegetation) in the OG streets. The studied soils did not show important changes due to LMC in their physical properties for short term, in addition, these soils were characterized by low organic matter content (<1.2%). LMC caused a SOC reduction in surface, and a SOC increase in the Bw horizon. The N concentrations showed a similar trend to SOC and the C:N ratios were highly variable (4.37: C horizon-NT-S; 13.45 Bw/C horizon -CT-B). Normally, the SOC-S concentrations decreasing in depth. LMC for two years showed soil carbonization (S and T position) and decarbonization (B position) processes. The SOC-S increased 1.88 Mg ha-1 y-1 and 0.47 Mg ha-1 y-1 for S and T topographic position respectively, however the SOC-S decreased in B position 5.27 Mg ha-1 y-1. Therefore, LMC has a positive effect on soil carbon reserves in S and T position, conversely in B position, this effect was negative.

Temporal and spatial variability of chemical and isotopic composition of soil solutions from cambisols - field study and experiments.

The chemical and isotopic composition of soil solutions is highly relevant for environmental and forensic tasks. We investigated interstitial solutions from soil horizons of three cambisols in Styria (Austria). The soils consisted mainly of quartz, feldspar and clay minerals with a vertical variability. Two soil solution fractions from meso-, macro- and micropores (m) and micropores only (µ) were extracted at two subsequent hydraulic pressure steps corresponding to matrix potentials of up to pF 5.43 and from 5.43 to 5.73, respectively. While solute concentrations indicated diverse distribution in soil solution fractions m and µ, heavy stable hydrogen and oxygen isotopes of H2O (-92.5‰<δ2H<-34.4‰; -11.9‰<δ18O<-4.0‰, VSMOW) are clearly enriched in the µ versus m fractions. Principal component analysis on the hydrochemical data set indicates that the intensity of the overall silicate weathering is higher in autumn versus spring, whereas the anthropogenic impact on weathering behaves inversely. The anthropogenic impact is related to seasonal variability of nitrification of N-fertilizers. In consequence of evaluated signals for overall silicate weathering about three-fourths of the soil solutions sampled in autumn indicated elevated total dissolved solid concentration vs. those in spring accompanied with washing out solutes from the soil cover following precipitation events in autumn before sampling. Isotopic shift of soil solutions from the local meteoric water line in spring obviously followed an evaporation trend because of less precipitation and high evaporation before sampling. Experimentally simulated evaporation of soil samples confirmed the observed isotopic evaporation trend. Wetting experiments indicated the infiltration of water within minutes into the micropores of the soils. Exchange of water molecules between micro-, meso- and macropores is an almost instantaneous process and soil solutions in micropores are not as isolated from the soil water system as it was formerly suggested, e.g. for plant uptake. Highly dynamic and complex mechanisms in the gas-water-solid system of soils have to be considered for the application of elemental and isotope proxies related to environmental, forensic and agricultural tasks.

Glyphosate and AMPA adsorption in soils: laboratory experiments and pedotransfer rules.

Adsorption of the herbicide glyphosate and its main metabolite AMPA (aminomethylphosphonic acid) was investigated on 17 different agricultural soils. Batch equilibration adsorption data are shown by Freundlich adsorption isotherms. Glyphosate adsorption is clearly affected by equilibration concentrations, but the nonlinear AMPA adsorption isotherms indicate saturation of the adsorption sites with increasing equilibrium concentrations. pHCaCl2 (i.e. experimental pH) is the major parameter governing glyphosate and AMPA adsorption in soils. However, considering pHCaCl2 values, available phosphate amount, and amorphous iron and aluminium oxide contents by using a nonlinear multiple regression equation, obtains the most accurate and powerful pedotransfer rule for predicting the adsorption constants for these two molecules. As amorphous iron and aluminium oxide contents in soil are not systematically determined, we also propose a pedotransfer rule with two variables-pHCaCl2 values and available phosphate amount-that remains acceptable for both molecules. Moreover, the use of the commonly measured pHwater or pHKCl values gives less accurate results compared to pHCaCl2 measurements. To our knowledge, this study is the first AMPA adsorption characterization for a significant number of temperate climate soils.

Microbial responses to southward and northward Cambisol soil transplant.

Soil transplant serves as a proxy to simulate climate changes. Recently, we have shown that southward transplant of black soil and northward transplant of red soil altered soil microbial communities and biogeochemical variables. However, fundamental differences in soil types have prevented direct comparison between southward and northward transplants. To tackle it, herein we report an analysis of microbial communities of Cambisol soil in an agriculture field after 4 years of adaptation to southward and northward soil transplants over large transects. Analysis of bare fallow soils revealed concurrent increase in microbial functional diversity and coarse-scale taxonomic diversity at both transplanted sites, as detected by GeoChip 3.0 and DGGE, respectively. Furthermore, a correlation between microbial functional diversity and taxonomic diversity was detected, which was masked in maize cropped soils. Mean annual temperature, soil moisture, and nitrate (NO3 ¯-N) showed strong correlations with microbial communities. In addition, abundances of ammonium-oxidizing genes (amoA) and denitrification genes were correlated with nitrification capacity and NO3 ¯-N contents, suggesting that microbial responses to soil transplant could alter microbe-mediated biogeochemical cycle at the ecosystem level.

Long-term combined chemical and manure fertilizations increase soil organic carbon and total nitrogen in aggregate fractions at three typical cropland soils in China.

Soil organic carbon (SOC), total nitrogen (TN), microbial biomass carbon (MBC) and nitrogen (MBN) are important factors of soil fertility. However, effects of the combined chemical fertilizer and organic manure or straw on these factors and their relationships are less addressed under long-term fertilizations. This study addressed changes in SOC, TN, MBC and MBN at 0-20 cm soil depth under three 17 years (September 1990-September 2007) long-term fertilization croplands along a heat and water gradient in China. Four soil physical fractions (coarse free and fine free particulate organic C, cfPOC and ffPOC; intra-microaggregate POC, iPOC; and mineral associated organic C, MOC) were examined under five fertilizations: unfertilized control, chemical nitrogen (N), phosphorus (P) and potassium (K) (NPK), NPK plus straw (NPKS, hereafter straw return), and NPK plus manure (NPKM and 1.5NPKM, hereafter manure). Compared with Control, manure significantly increased all tested parameters. SOC and TN in fractions distributed as MOC > iPOC > cfPOC > ffPOC with the highest increase in cfPOC (329.3%) and cfPTN (431.1%), and the lowest in MOC (40.8%) and MTN (45.4%) under manure. SOC significantly positively correlated with MBC, cfPOC, ffPOC, iPOC and MOC (R(2) = 0.51-0.84, P < 0.01), while TN with cfPTN, ffPTN, iPTN and MTN (R(2) = 0.45-0.79, P < 0.01), but not with MBN, respectively. Principal component analyses explained 86.9-91.2% variance of SOC, TN, MBC, MBN, SOC and TN in each fraction. Our results demonstrated that cfPOC was a sensitive SOC indicator and manure addition was the best fertilization for improving soil fertility while straw return should take into account climate factors in Chinese croplands.

Long-term fertilisation causes excess supply and loss of phosphorus in purple paddy soil.

BACKGROUND: Phosphorus (P) loss from cropland is accelerating the eutrophication of waters around the world such as the Three Gorges Reservoir (TGR) in China. We investigated whether purple paddy soil under long-term P applications was a major source of P efflux to the TGR. RESULTS: Substantial surplus P in the plough layer (0-20 cm) was evident after 15-year P applications. Available P (Olsen-P) in the plough layer ranged from 1.9 to 42.4 mg kg(-1) and some of which will exceed the threshold of 30 mg kg(-1) for environmental concern within 7 years of P application (inorganic NPK with or without organic fertiliser). Between 30 and 70% of Olsen-P was leached out of the 0-30 cm soil layer. Surplus P resulted in high P concentrations in the surface water during the rice cropping season, and these concentrations exceeded those in most waters of the TGR and exceeded the critical level for eutrophication (0.1 mg L(-1)) during the first 10 days after rice planting. Furthermore, total P in run-off due to rainfall events exceeded the level for eutrophication, with a total loss of 43.2-147.9 g P ha(-1) depending on the fertilisation. CONCLUSION: Current agronomic P management in purple paddy soil is environmentally unsustainable in terms of the adverse impact on surface water quality. Integrated P management practices are urgently required to optimise crop yield while minimising P loss in order to protect surface water quality in the TGR region.

Teores de nutrientes na parte aérea da batateira em resposta ao boro / Nutrient contents of the potato shoot plants in response of to the boron levels

O trabalho foi desenvolvido com o objetivo de avaliar as alterações nos teores e acúmulo de nutrientes da parte aérea da batateira, em função de doses de boro em amostras de um Latossolo Vermelho (LV) e de um Cambissolo (CX). O delineamento experimental foi em blocos casualizados (DBC) em esquema fatorial 4 x 2, constituído de quatro doses de B (0,0; 0,75; 1,50 e 3,0 mg dm-3) e duas cultivares de batata (Asterix e Monalisa), 2 tipos de solos (Latossolo e Cambissolo) e três repetições. Foram avaliados os teores de N, P, P Ca, Mg, S, B, Cu,Fe, Mn, e Zn nas folhas e acúmulo na parte aérea de cada cultivar. Os tratamentos de forma individual e/ou em interação influenciaram tanto os teores como o acúmulo dos nutrientes na batateira. Dependendo do tipo de solo e cultivar, houve influencia sobre os teores de Mg, P e Mn nas folhas e o acúmulo de N, P, K, Ca, Mg, S, B, Cu, Fe, Mn e Zn na parte aérea. As doses de B, independentemente das cultivares, elevaram o teor e acúmulo de B e reduziram os de S, Cu, Fe e Zn, conforme o tipo de solo. Promoveram também, conforme o tipo de solo, aumento nos teores K, B e Fe e acúmulo de S na cultivar Asterix e, aumento nos teores de P e B, acúmulo de N, Cu, e Zn e redução nos teores de K, Ca, Mn e Fe na cultivar Monalisa.

The study was carried out to evaluate the nutrient content alterations of the potato shoots submitted to boron rates. The experiment had a randomized blocks outline in 4 x 3 factorial arrangement, using four boron rates (0.0, 0.75; 1.50 and 3.0 mg dm-3) and two potato cultivars (Asterix and Monalisa) with three replicates. Two soil types were used, Latosol and Cambisol. Leaf and shoots N, P, K, Ca, Mg, S, Fe, Cu, Mn, Zn and B contents of each cultivar were evaluated. The treatments as in individual or in interaction form influenced the potatoes nutrient content and its nutrient accumulation on the plant. Depending on the soil type Mg, P and Mn content were influenced on the leaves and the N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn accumulation content on the aerial part. Depending on soil type and cultivar there was influence on the Mg, P and Mn content on the leaves and accumulation of the N, P, K, Ca, Mg, S, B, Cu, Fe, Mn and Zn in aerial part. The B rates independely from cultivars elevated the boron content and accumulation and reduced the S, Cu, Fe and Zn contents according to soil type. They also promoted according to soil type, increases on K, B and Fe and S accumulation on Asterix cultivar and, increases on and P and B content, and accumulation of N, Cu, and Zn and content reduction on K, Ca, Mn and Fe, on Monalisa cultivar.