Foliar Spraying with ZnSO4 or ZnO of Vitis vinifera cv. Syrah Increases the Synthesis of Photoassimilates and Favors Winemaking.

Zinc enrichment of edible food products, through the soil and/or foliar application of fertilizers, is a strategy that can increase the contents of some nutrients, namely Zn. In this context, a workflow for agronomic enrichment with zinc was carried out on irrigated Vitis vinifera cv. Syrah, aiming to evaluate the mobilization of photoassimilates to the winegrapes and the consequences of this for winemaking. During three productive cycles, foliar applications were performed with ZnSO4 or ZnO, at concentrations ranging between 150 and 1350 g.ha-1. The normal vegetation index as well as some photosynthetic parameters indicated that the threshold of Zn toxicity was not reached; it is even worth noting that with ZnSO4, a significant increase in several cases was observed in net photosynthesis (Pn). At harvest, Zn biofortification reached a 1.2 to 2.3-fold increase with ZnSO4 and ZnO, respectively (being significant relative to the control, in two consecutive years, with ZnO at a concentration of 1350 g.ha-1). Total soluble sugars revealed higher values with grapes submitted to ZnSO4 and ZnO foliar applications, which can be advantageous for winemaking. It was concluded that foliar spraying was efficient with ZnO and ZnSO4, showing potential benefits for wine quality without evidencing negative impacts.

The sound of lichens: ultrasonic acoustic emissions during desiccation question cavitation events in the hyphae.

Lichens are a mutualistic symbiosis between a fungus and one or more photosynthetic partners. They are photosynthetically active during desiccation until relative water contents (RWC) as low as 30% (on dry mass). Experimental evidence suggests that during desiccation, the photobionts have a higher hydration level than the surrounding fungal pseudo-tissues. Explosive cavitation events in the hyphae might cause water movements towards the photobionts. This hypothesis was tested in two foliose lichens by measurements of ultrasonic acoustic emissions (UAE), a method commonly used in vascular plants but never in lichens, and by measurements of photosystem II efficiency, water potential and RWC. Thallus structural changes were characterised by low-temperature scanning electron microscopy. The thalli were silent between 380% and 30% RWCs, i.e. when explosive cavitation events should cause movements of liquid water. Nevertheless, the thalli emitted UAE at approximately 5% RWC. Accordingly, the medullary hyphae were partially shrunk at about 15% RWC, whereas they were completely shrunk below 5% RWC. These results do not support the hypothesis of hyphal cavitation and suggest that the UAE originate from structural changes at hyphal level. The shrinking of hyphae is proposed as an adaptation to avoid cell damage at very low RWCs.

Photosynthetic Performance and Yield Losses of Winter Rapeseed (Brassica napus L. var. napus) Caused by Simulated Hail.

Winter oilseed rape (Brassica napus L.), Europe's foremost oilseed crop, is significantly impacted by hailstorms, leading to substantial yield reductions that are difficult to predict and measure using conventional methods. This research aimed to assess the effectiveness of photosynthetic efficiency analysis for predicting yield loss in winter rapeseed subjected to hail exposure. The aim was to pinpoint the chlorophyll fluorescence parameters most affected by hail stress and identify those that could act as non-invasive biomarkers of yield loss. The study was conducted in partially controlled conditions (greenhouse). Stress was induced in the plants by firing plastic balls with a 6 mm diameter at them using a pneumatic device, which launched the projectiles at speeds of several tens of meters per second. Measurements of both continuous-excitation and pulse-modulated-amplitude chlorophyll fluorescence were engaged to highlight the sensitivity of the induction curve and related parameters to hail stress. Our research uncovered that some parameters such as Fs, Fm', ΦPSII, ETR, Fo, Fv/Fm, and Fv/Fo measured eight days after the application of stress had a strong correlation with final yield, thus laying the groundwork for the creation of new practical protocols in agriculture and the insurance industry to accurately forecast damage to rapeseed crops due to hail stress.

Screening rate constants in the simulation of rapid kinetics of chlorophyll a fluorescence using the Morris method.

Chlorophyll a fluorescence, a sensitive and cost-effective probe, is widely used in photosynthetic research. Its rapid phase, occurring within 1 second under intense illumination, displays complex O-J-I-P transients, providing valuable insights into various aspects of photosynthesis. In addition to employing experimental approaches to measure the rapid Fluorescence Induction (FI) kinetics, mathematical modeling serves as a crucial tool for understanding the underlying mechanisms that drive FI dynamics. However, the significant uncertainty and arbitrary nature of selecting model parameters amplify concerns about the effectiveness of modeling tools in aiding photosynthesis research. Therefore, there is a need to gain a deeper understanding of how these models operate and how arbitrary parameter choices may influence their outcomes. In this study, we employed the Morris method, a global Sensitivity Analysis (SA) tool, to assess the significance of rate constants employed in an existing fluorescence model, particularly those linked to the entire electron transport chain, in shaping the rapid FI dynamics. In summary, utilizing the insights gained from the Morris SA allows for targeted refinement of the photosynthesis model, thereby improving our understanding of the complex processes inherent in photosynthetic systems.

Cold Acclimation and Deacclimation of Winter Oilseed Rape, with Special Attention Being Paid to the Role of Brassinosteroids.

Winter plants acclimate to frost mainly during the autumn months, through the process of cold acclimation. Global climate change is causing changes in weather patterns such as the occurrence of warmer periods during late autumn or in winter. An increase in temperature after cold acclimation can decrease frost tolerance, which is particularly dangerous for winter crops. The aim of this study was to investigate the role of brassinosteroids (BRs) and BR analogues as protective agents against the negative results of deacclimation. Plants were cold-acclimated (3 weeks, 4 °C) and deacclimated (1 week, 16/9 °C d/n). Deacclimation generally reversed the cold-induced changes in the level of the putative brassinosteroid receptor protein (BRI1), the expression of BR-induced COR, and the expression of SERK1, which is involved in BR signal transduction. The deacclimation-induced decrease in frost tolerance in oilseed rape could to some extent be limited by applying steroid regulators. The deacclimation in plants could be detected using non-invasive measurements such as leaf reflectance, chlorophyll a fluorescence, and gas exchange monitoring.

Individual architecture and photosynthetic performance of the submerged form of Drosera intermedia Hayne.

Drosera intermedia grows in acidic bogs in parts of valleys that are flooded in winter, and that often dry out in summer. It is also described as the sundew of the most heavily hydrated habitats in peatlands, and it is often found in water and even underwater. This sundew is the only one that can tolerate long periods of submersion, and more importantly produces a typical submerged form that can live in such conditions for many years. Submerged habitats are occupied by D. intermedia relatively frequently. The aim of the study was to determine the environmental conditions and architecture of individuals in the submerged form of D. intermedia. The features of the morphological and anatomical structure and chlorophyll a fluorescence of this form that were measured were compared with analogous ones in individuals that occurred in emerged and peatland habitats. The submerged form occurred to a depth of 20 cm. Compared to the other forms, its habitat had the highest pH (4.71-4.92; Me = 4.71), the highest temperature and substrate hydration, and above all, the lowest photosynthetically active radiation (PAR; 20.4-59.4%). This form differed from the other forms in almost all of the features of the plant's architecture. It is particularly noteworthy that it had the largest main axis height among all of the forms, which exceeded 18 cm. The number of living leaves in a rosette was notable (18.1 ± 8.1), while the number of dead leaves was very low (6.9 ± 3.8). The most significant differences were in the shape of its submerged leaves, in which the length of the leaf blade was the lowest of all of the forms (0.493 ± 0.15 mm; p < 0.001) and usually the widest. The stem cross-sectional area was noticeably smaller in the submerged form than in the other forms, the xylem was less developed and collaterally closed vascular bundles occurred. Our analysis of the parameters of chlorophyll fluorescence in vivo revealed that the maximum quantum yield of the primary photochemistry of photosystem II is the highest for the submerged form (Me = 0.681), the same as the maximum quantum yield of the electron transport (Me φE0 = 0.183). The efficiency of energy use per one active reaction center of photosystem II (RC) was the lowest in the submerged form (Me = 2.978), same as the fraction of energy trapped by one active RC (Me = 1.976) and the non-photochemical energy dissipation (DI0/RC; Me = 0.916). The ET0/RC parameter, associated with the efficiency of the energy utilization for electron transport by one RC, in the submerged plant reached the highest value (Me = 0.489). The submerged form of D. intermedia clearly differed from the emerged and peatland forms in its plant architecture. The submerged plants had a thinner leaf blade and less developed xylem than the other forms, however, their stems were much longer. The relatively high photosynthetic efficiency of the submerged forms suggests that most of the trapped energy is utilized to drive photosynthesis with a minimum energy loss, which may be a mechanism to compensate for the relatively small size of the leaf blade.

Crop-Specific Responses to Cold Stress and Priming: Insights from Chlorophyll Fluorescence and Spectral Reflectance Analysis in Maize and Soybean.

This study aimed to investigate the impact of cold stress and priming on photosynthesis in the early development of maize and soybean, crops with diverse photosynthetic pathways. The main objectives were to determine the effect of cold stress on chlorophyll a fluorescence parameters and spectral reflectance indices, to determine the effect of cold stress priming and possible stress memory and to determine the relationship between different parameters used in determining the stress response. Fourteen maize inbred lines and twelve soybean cultivars were subjected to control, cold stress, and priming followed by cold stress in a walk-in growth chamber. Measurements were conducted using a portable fluorometer and a handheld reflectance instrument. Cold stress induced an overall downregulation of PSII-related specific energy fluxes and efficiencies, the inactivation of RCs resulting in higher energy dissipation, and electron transport chain impairment in both crops. Spectral reflectance indices suggested cold stress resulted in pigment differences between crops. The effect of priming was more pronounced in maize than in soybean with mostly a cumulatively negative effect. However, priming stabilized the electron trapping efficiency and upregulated the electron transfer system in maize, indicating an adaptive response. Overall, this comprehensive analysis provides insights into the complex physiological responses of maize and soybean to cold stress, emphasizing the need for further genotype-specific cold stress response and priming effect research.

Light Response Curves in Land Plants.

Light is the driving force for photosynthesis. Two techniques are commonly employed to help characterize the relationship between the light environment and photosynthesis in plants.Chlorophyll a fluorescence analysis is used to examine both the capacity for and the efficiency of the conversion of absorbed light into energy for photosynthesis. Additionally, gas exchange analysis is used to assess the utilization of that energy for carbon fixation. These techniques are used either in isolation or in combination to acquire light response curves that measure the response of the plant to sequential changes in irradiance. Light response curves can help users understand photosynthetic mechanisms, evaluate how plants respond to light conditions, or assess the extent of physiological plasticity within plants. In this chapter, we provide a generalized method for acquiring light response curves suitable for both chlorophyll a fluorescence and gas exchange techniques using commercially available apparatus. Depending on the equipment available, these methods can be applied individually or combined to acquire data simultaneously. The methods are broadly applicable to most land plants but are ideally suited to help those that are unfamiliar with these techniques.

Effects of lipids on the rate-limiting steps in the dark-to-light transition of Photosystem II core complex of Thermostichus vulcanus.

In our earlier works, we have shown that the rate-limiting steps, associated with the dark-to-light transition of Photosystem II (PSII), reflecting the photochemical activity and structural dynamics of the reaction center complex, depend largely on the lipidic environment of the protein matrix. Using chlorophyll-a fluorescence transients (ChlF) elicited by single-turnover saturating flashes, it was shown that the half-waiting time (Δτ 1/2) between consecutive excitations, at which 50% of the fluorescence increment was reached, was considerably larger in isolated PSII complexes of Thermostichus (T.) vulcanus than in the native thylakoid membrane (TM). Further, it was shown that the addition of a TM lipid extract shortened Δτ 1/2 of isolated PSII, indicating that at least a fraction of the 'missing' lipid molecules, replaced by detergent molecules, caused the elongation of Δτ 1/2. Here, we performed systematic experiments to obtain information on the nature of TM lipids that are capable of decreasing Δτ 1/2. Our data show that while all lipid species shorten Δτ 1/2, the negatively charged lipid phosphatidylglycerol appears to be the most efficient species - suggesting its prominent role in determining the structural dynamics of PSII reaction center.

Variation in leaf carbon economics, energy balance, and heat tolerance traits highlights differing timescales of adaptation and acclimation.

Multivariate leaf trait correlations are hypothesized to originate from natural selection on carbon economics traits that control lifetime leaf carbon gain, and energy balance traits governing leaf temperatures, physiological rates, and heat injury. However, it is unclear whether macroevolution of leaf traits primarily reflects selection for lifetime carbon gain or energy balance, and whether photosynthetic heat tolerance is coordinated along these axes. To evaluate these hypotheses, we measured carbon economics, energy balance, and photosynthetic heat tolerance traits for 177 species (157 families) in a common garden that minimizes co-variation of taxa and climate. We observed wide variation in carbon economics, energy balance, and heat tolerance traits. Carbon economics and energy balance (but not heat tolerance) traits were phylogenetically structured, suggesting macroevolution of leaf mass per area and leaf dry matter content reflects selection on carbon gain rather than energy balance. Carbon economics and energy balance traits varied along a common axis orthogonal to heat tolerance traits. Our results highlight a fundamental mismatch in the timescales over which morphological and heat tolerance traits respond to environmental variation. Whereas carbon economics and energy balance traits are constrained by species' evolutionary histories, photosynthetic heat tolerance traits are not and can acclimate readily to leaf microclimates.

Promoting effect of low concentration strontium on photosynthetic performance of Chinese cabbage seedlings: Combined leaf characteristics, photosynthetic carbon assimilation and chlorophyll fluorescence.

Low concentration strontium (LC-Sr) can promote the growth of plants. In order to explore its promoting mechanism from the aspect of photosynthesis, the leaf characteristics, CO2 assimilation and chlorophyll (Chl) a fluorescence kinetics were investigated with hydroponically LC-Sr-treated Chinese cabbage seedlings. After a 28-d treatment to SrCl2 at different concentrations (0.1, 0.2, 0.5, and 1.0 mmol L-1), we observed an increase in the specific leaf weight (SLW) of Chinese cabbage compared with the control group. Notably, as the strontium concentration increased, a more pronounced improvement trend in the contents of Chl and protein in the leaves was observed, contributing to the enhancement of photosynthesis. However, the statistical differences in Pn among various LC-Sr treatments were not significant. Nevertheless, the leaf starch content exhibited a significant increase after LC-Sr treatments. Additionally, Chl a fluorescence transient has been used as a sensitive indicator of the promotional effect of LC-Sr on photosynthesis. The results of fluorescence parameters showed that LC-Sr treatments accelerated the light reaction speed of leaves (Tfm, dV/dto, dVG/dto), improved the energy utilization efficiency of photosystem (PSI and PSII) (ETo/CSo, ψET,ψRE, δRo, φRo), and ultimately enhanced the photosynthetic performance of leaves (PIabs, SFIabs, DFabs). The increased RCs/CSo and Sm contributed to the enhancement of the light reaction activity of strontium-treated leaves. The LC-Sr treatments had no interference with the calcium absorption, and notably enhanced the photosynthetic capacity of Chinese cabbage, shedding light on potential benefits of LC-Sr for crop cultivation.

Mechanisms of cadmium mitigation in tomato plants under orthophosphate and polyphosphate fertilization regimes.

Cadmium (Cd) is one of the most toxic elements in soil, affecting morphological, physiological, and biochemical processes in plants. Mineral plant nutrition was tested as an effective approach to mitigate Cd stress in several crop species. In this regard, the present study aimed to elucidate how different phosphorus (P) fertilization regimes can improve some bio-physiological processes in tomato plants exposed to Cd stress. In a hydroponic experiment, the impact of two phosphorus fertilizer forms (Polyphosphate (poly-P): condensed P-form with 100% polymerization rate and orthophosphate (ortho-P): from orthophosphoric acid) on the photosynthetic activity, plant growth, and nutrient uptake was assessed under three levels of Cd stress (0, 12, and 25 µM of CdCl2). The obtained results confirmed the negative effects of Cd stress on the chlorophyll content and the efficiency of the photosynthesis machinery. The application of poly-P fertilizer significantly improved the chlorophyll stability index (82%) under medium Cd stress (Cd12), as compared to the ortho-P form (55%). The analysis of the chlorophyll α fluorescence transient curve revealed that the amplitude of Cd effect on the different steps of electron transfer between PSII and PSI was significantly reduced under the poly-P fertilization regime compared to ortho-P, especially under Cd12. The evaluation of the RE0/RC parameter showed that the electron flux reducing end electron acceptors at the PSI acceptor side per reaction center was significantly improved in the poly-P treatment by 42% under Cd12 compared to the ortho-P treatment. Moreover, the use of poly-P fertilizer enhanced iron uptake and its stoichiometric homeostasis in the shoot tissue which maintained an adequate absorption of iron under Cd stress conditions. Findings from this study revealed for the first time that inorganic polyphosphate fertilizers can reduce Cd toxicity in tomato plants by enhancing photosynthesis activity, nutrient uptake, plant growth, and biomass accumulation despite the high level of cadmium accumulation in shoot tissues.

Photosynthetic Responses of Racomitrium japonicum L. to Strontium Stress Evaluated through Chlorophyll a Fluorescence OJIP Transient Analysis.

Nuclides pollution and its biological effects are of great concern, especially for bryophytes during their terrestrial adaptation. Understanding PSII activity and electron transport response is vital for comprehending moss abiotic stress reactions. However, little is known about the photosynthetic performance of moss under nuclide treatment. Therefore, this study aimed to evaluate the chlorophyll fluorescence of Racomitrium japonicum L. The moss was subjected to Sr2+ solutions at concentrations of 5, 50, and 500 mg/L to evaluate chlorophyll a fluorescence using the OJIP test. Moderate and high Sr2+ stress led to inner cell membrane dissolution and reduced chlorophyll content, indicating impaired light energy absorption. At 5 mg/L Sr2+, fluorescence kinetics showed increased light energy capture, energy dissipation, and total photosynthetic driving force, thus stimulating transient photosynthetic activity of PSII and improving PSI reduction. Linear electron transfer and PSII stability significantly decreased under moderate and high Sr2+ stress, indicating potential photosynthetic center damage. Cyclic electron transfer (CEF) alleviated photosynthetic stress at 5 mg/L Sr2+. Thus, low Sr2+ levels stimulated CEF, adjusting energy flux and partitioning to protect the photosynthetic apparatus. Nevertheless, significant damage occurred due to inefficient protection under high Sr2+ stress.

Effects of Simulated Acid Rain on Photosynthesis in Pinus massoniana and Cunninghamia lanceolata in Terms of Prompt Fluorescence, Delayed Fluorescence, and Modulated Reflection at 820 nm.

The effects of simulated acid rain (SAR) on the photosynthetic performance of subtropical coniferous species have not been thoroughly investigated. In this study, we treated two coniferous species, Pinus massoniana (PM) and Cunninghamia lanceolata (CL), with four gradients of SAR and then analyzed their photosynthetic activities through measurements of gas exchange, prompt fluorescence (PF), delayed fluorescence (DF), and modulated reflection at 820 nm (MR820). Gas exchange analysis indicated that the decrease in the net photosynthetic rate (Pn) in PM and CL was unrelated to stomatal factors. For the PF transients, SAR induced positive K-band and L-band, a significant reduction in photosynthetic performance index (PIABS), the quantum yield of electron transfer per unit cross-section (ETO/CSm), and maximal photochemical efficiency of photosystem II (Fv/Fm). Analysis of the MR820 kinetics showed that the re-reduction kinetics of PSI reaction center (P700+) and plastocyanin (PC+) became slower and occurred at later times under SAR treatment. For the DF signals, a decrease in the amplitude of the DF induction curve reduced the maximum value of DF (I1). These results suggested that SAR obstructed photosystem II (PSII) donor-side and acceptor-side electron transfer capacity, impaired the connectivity between PSII and PSI, and destroyed the oxygen-evolving complex (OEC). However, PM was better able to withstand SAR stress than CL, likely because of the activation of a protective mechanism.

Comparison of Growth and Physiological Effects of Soil Moisture Regime on Plantago maritima Plants from Geographically Isolated Sites on the Eastern Coast of the Baltic Sea.

The aim of the present study was to evaluate the morphological and physiological responses of P. maritima plants from five geographically isolated sites growing in habitats with different conditions to different substrate moisture levels in controlled conditions. Plants were produced from seed and cultivated in a greenhouse at four relatively constant soil moisture regimes: at 25, 50, and 75% soil water content and in soil flooded 3 cm above the surface (80% F). The two morphological traits that varied most strikingly among P. maritima accessions were the number of flower stalks and the number of leaves. Only plants from two accessions uniformly produced generative structures, and allocation to flowering was suppressed by both low moisture and flooding. Optimum shoot biomass accumulation for all accessions was at 50 and 75% soil moisture. The Performance Index Total was the most sensitive among the measured photosynthesis-related parameters, and it tended to decrease with an increase in soil water content for all P. maritima accessions. The initial hypothesis-that plants from relatively dry habitats will have a higher tolerance against low soil water levels, but plants from relatively wet habitats will have a higher tolerance against waterlogged or flooded soil-was not proven. The existence of three ecotypes of P. maritima within the five accessions from geographically isolated subpopulations on the eastern coast of the Baltic Sea at the level of morphological responses to soil water content can be proposed. P. maritima plants can be characterized as extremely tolerant to soil waterlogging and highly tolerant to soil flooding and low soil water content.

Comparative modeling of fluorescence and P700 induction kinetics for alga Scenedesmus sp. obliques and cyanobacterium Synechocystis sp. PCC 6803. Role of state 2-state 1 transitions and redox state of plastoquinone pool.

The model of thylakoid membrane system (T-M model) (Belyaeva et al. Photosynth Res 2019, 140:1-19) has been improved in order to analyze the induction data for dark-adapted samples of algal (Scenedesmus obliques) and cyanobacterial (Synechocystis sp. PCC 6803) cells. The fluorescence induction (FI) curves of Scenedesmus were measured at light exposures of 5 min, while FI and P700 redox transformations of Synechocystis were recorded in parallel for 100 s intervals. Kinetic data comprising the OJIP-SMT fluorescence induction and OABCDEF P700+ absorbance changes were used to study the processes underlying state transitions qT2→1 and qT1→2 associated with the increase/decrease in Chl fluorescence emission. A formula with the Hill kinetics (Ebenhöh et al. Philos Trans R Soc B 2014, 369:20130223) was introduced into the T-M model, with a new variable to imitate the flexible size of antenna AntM(t) associated with PSII. Simulations revealed that the light-harvesting capacity of PSII increases with a corresponding decrease for that of PSI upon the qT2→1 transition induced by plastoquinone (PQ) pool oxidation. The complete T-M model fittings were attained on Scenedesmus or Synechocystis fast waves OJIPS of FI, while SMT wave of FI was reproduced at intervals shorter than 5 min. Also the fast P700 redox transitions (OABC) for Synechocystis were fitted exactly. Reasonable sets of algal and cyanobacterial electron/proton transfer (ET/PT) parameters were found. In the case of Scenedesmus, ET/PT traits remained the same irrespective of modeling with or without qT2→1 transitions. Simulations indicated a high extent (20%) of the PQ pool reduction under dark conditions in Synechocystis compared to 2% in Scenedesmus.

Compensatory growth of Microcystis aeruginosa after copper stress and the characteristics of algal extracellular organic matter (EOM).

Cyanobacterial blooms can impair drinking water quality due to the concomitant extracellular organic matter (EOM). As copper is often applied as an algicide, cyanobacteria may experience copper stress. However, it remains uncertain whether algal growth compensation occurs and how EOM characteristics change in response to copper stress. This study investigated the changes in growth conditions, photosynthetic capacity, and EOM characteristics of M. aeruginosa under copper stress. In all copper treatments, M. aeruginosa experienced a growth inhibition stage followed by a growth compensation stage. Notably, although chlorophyll-a fluorescence parameters dropped to zero immediately following high-intensity copper stress (0.2 and 0.5 mg/L), they later recovered to levels exceeding those of the control, indicating that photosystem II was not destroyed by copper stress. Copper stress influenced the dissolved organic carbon (DOC) content, polysaccharides, proteins, excitation-emission matrix spectra, hydrophobicity, and molecular weight (MW) distribution of EOM, with the effects varying based on stress intensity and growth stage. Principal component analysis revealed a correlation between the chlorophyll-a fluorescence parameters and EOM characteristics. These results imply that copper may not be an ideal algicide. Further research is needed to explore the dynamic response of EOM characteristics to environmental stress.

Psammophytes Alyssum desertorum Stapf and Secale sylvestre Host Are Sensitive to Soil Flooding.

While morphological and functional traits enable hydrophytes to survive under waterlogging and partial or complete submergence, the data on responses of psammophytes-sand plants-to flooding are very limited. We analyzed the effect of 5- and 10-day soil flooding on the photosynthetic apparatus and the synthesis of alcohol dehydrogenase (ADH), heat shock proteins 70 (HSP70), and ethylene in seedlings of psammophytes Alyssum desertorum and Secale sylvestre using electron microscopy, chlorophyll a fluorescence induction, and biochemical methods. It was found that seedlings growing under soil flooding differed from those growing in stationary conditions with such traits as chloroplast ultrastructure, pigment content, chlorophyll fluorescence induction, and the dynamics of ADH, HSP, and ethylene synthesis. Although flooding caused no apparent damage to the photosynthetic apparatus in all the variants, a significant decrease in total photosynthesis efficiency was observed in both studied plants, as indicated by decreased values of φR0 and PIABS,total. More noticeable upregulation of ADH in S. sylvestre, as well as increasing HSP70 level and more intensive ethylene emission in A. desertorum, indicate species-specific differences in these traits in response to short-term soil flooding. Meanwhile, the absence of systemic anaerobic metabolic adaptation to prolonged hypoxia causes plant death.

Heat Pre-Treatment Modified Host and Non-Host Interactions of Powdery Mildew with Barley Brassinosteroid Mutants and Wild Types.

High temperatures associated with climate change may increase the severity of plant diseases. This study investigated the effect of heat shock treatment on host and non-host barley powdery mildew interactions using brassinosteroid (BR) mutants of barley. Brassinosteroids are plant steroid hormones, but so far little is known about their role in plant-fungal interactions. Wild type barley cultivar Bowman and its near-isogenic lines with disturbances in BR biosynthesis or signalling showed high compatibility to barley powdery mildew race A6, while cultivar Delisa and its BR-deficient mutants 522DK and 527DK were fully incompatible with this pathogen (host plant-pathogen interactions). On the other hand, Bowman and its mutants were highly resistant to wheat powdery mildew, representing non-host plant-pathogen interactions. Heat pre-treatment induced shifts in these plant-pathogen interactions towards higher susceptibility. In agreement with the more severe disease symptoms, light microscopy showed a decrease in papillae formation and hypersensitive response, characteristic of incompatible interactions, when heat pre-treatment was applied. Mutant 527DK, but not 522DK, maintained high resistance to barley powdery mildew race A6 despite heat pre-treatment. By 10 days after heat treatment and infection, a noticeable shift became apparent in the chlorophyll a fluorescence and in various leaf reflectance parameters at all genotypes.

Effect of Na, K and Ca Salts on Growth, Physiological Performance, Ion Accumulation and Mineral Nutrition of Mesembryanthemum crystallinum.

Mesembryanthemum crystallinum L. is an obligatory halophyte species showing optimum growth at elevated soil salinity levels, but the ionic requirements for growth stimulation are not known. The aim of the present study was to compare the effects of sodium, potassium and calcium in the form of chloride and nitrate salts on the growth, physiological performance, ion accumulation and mineral nutrition of M. crystallinum plants in controlled conditions. In a paradoxical way, while sodium and potassium had comparable stimulative effect on plant growth, the effect of calcium was strongly negative even at a relatively low concentration, eventually leading to plant death. Moreover, the effect of Ca nitrate was less negative in comparison to that of Ca chloride, but K in the form of nitrate had some negative effects. There were three components of the stimulation of biomass accumulation by NaCl and KCl salinity in M. crsytallinum: the increase in tissue water content, increase in ion accumulation, and growth activation. As optimum growth was in a salinity range from 20 to 100 mM, the increase in the dry biomass of plants at a moderate (200 mM) and high (400 mM) salinity in comparison to control plants was mostly due to ion accumulation. Among physiological indicators, changes in leaf chlorophyll concentration appeared relatively late, but the chlorophyll a fluorescence parameter, Performance Index Total, was the most sensitive to the effect of salts. In conclusion, both sodium and potassium in the form of chloride salts are efficient in promoting the optimum growth of M. crystallinum plants. However, mechanisms leading to the negative effect of calcium on plants need to be assessed further.