DRB1 locus alleles of HLA class II are associated with modulation of the immune response in different serological profiles of HIV-1/Epstein-Barr virus coinfection in the Brazilian Amazon region.

Background: Epstein-Barr virus (EBV) infection involves distinct clinical and serological profiles. We evaluated the frequency of alleles of locus DRB1 of HLA class II in different serological profiles of EBV infection among HIV-1 infected patients. Methods: We recruited 19 patients with primary infection, 90 with serological transition and 467 with past infection by EBV, HIV-1 co-infection was 100% in primary infection and approximately 70% in other serological profiles. EBV viral load was quantified by real-time PCR, T lymphocyte quantification and cytokine level analysis were performed by flow cytometry, and HLA locus genotyping was performed by PCR-SSO. Results: The DRB1*09 allele was associated with primary infection (p: 0.0477), and carriers of the allele showed changes in EBV viral load (p: 0.0485), CD8(+) T lymphocyte counts (p: 0.0206), double-positive T lymphocyte counts (p: 0.0093), IL-4 levels (p: 0.0464) and TNF levels (p: 0.0161). This allele was also frequent in HIV-coinfected individuals (p: 0.0023) and was related to the log10 HIV viral load (p: 0.0176) and CD8(+) T lymphocyte count (p: 0.0285). In primary infection, the log10 HIV viral load was high (p: 0.0060) and directly proportional to the EBV viral load (p: 0.0412). The DRB1*03 allele correlated with serological transition (p: 0.0477), EBV viral load (p: 0.0015), CD4(+) T lymphocyte count (p: 0.0112), CD8(+) T lymphocyte count (p: 0.0260), double-negative T lymphocyte count (p: 0.0540), IL-4 levels (p: 0.0478) and IL-6 levels (p: 0.0175). In the serological transition group, the log10 HIV viral load was high (p: 0.0060), but it was not associated with the EBV viral load (p: 0.1214). Past infection was related to the DRB1*16 allele (p: 0.0477), with carriers displaying IgG levels (p: 0.0020), CD4(+) T lymphocyte counts (p: 0.0116) and suggestive CD8(+) T count alterations (p: 0.0602). The DRB01*16 allele was also common in HIV-1 patients with past EBV infection (p: 0.0192); however, the allele was not associated with clinical markers of HIV-1 infection. Conclusion: Our results suggest that HLA class II alleles may be associated with the modulation of the serological profiles of the immune response to Epstein-Barr virus infection in patients coinfected with HIV-1.

HLA-DRB1 and HLA-DQB1 genes in patients diagnosed with systemic lupus erythematosus in Guatemala.

Systemic lupus erythematosus (SLE) is an autoimmune disorder that impacts connective tissue and can affect various organs and systems within the body. One important aspect of this disease is the role of the human leukocyte antigen (HLA) system, a protein complex that plays a role in the immune response. Specifically, the HLA-DRB1 and HLA-DQB1 genes have been implicated in the development of SLE. In order to better understand this relationship in the Guatemalan population, a study was conducted with the objective of characterizing the allelic and haplotype profiles of the HLA-DQB1 and HLA-DRB1 loci in 50 patients diagnosed with SLE who were receiving treatment at a hospital in Guatemala. Allele and haplotype frequencies were determined and compared to 127 healthy Guatemalan subjects as a control group. The results of the analysis showed a reduction in the frequencies of HLA-DQB1*03 and HLA-DRB1*14 in SLE patients, which could suggest a protective effect on the development of the disease. In contrast, a risk association was found between HLA-DRB1*07, HLA-DRB1*08, HLA-DQB1*02 and HLA-DQB1*06 in SLE patients. Finally, we observed an additional protective associated of haplotype HLA-DRB1*04∼DQB1*03 with SLE patients, while haplotypes HLA-DRB1*07∼DQB1*02 and DRB1*08-DQB1*06 showed a risk association.

Guía de atención para pacientes con maloclusión de clase II con dentición permanente en crecimiento activo

Fundamento la maloclusión de clase II presenta la mayor prevalencia entre las alteraciones dentoesqueléticas. Objetivo: proponer una guía de atención para pacientes con maloclusión de clase II con dentición permanente en crecimiento activo. Métodos: se realizó una investigación de innovación tecnológica de enfoque mixto en el Departamento de Ciencias Clínicas de la Facultad de Estomatología de la Universidad de Ciencias Médicas de Villa Clara, en el periodo comprendido entre los años 2020 y 2023. El universo de estudio estuvo constituido por todos los especialistas de Ortodoncia de la provincia de Villa Clara, Sancti Spíritus y Cienfuegos, a partir del cual se realizó un muestreo no probabilístico intencional por criterios y quedó constituida la muestra por 20 especialistas y siete expertos (especialistas de categoría superior) con criterios de inclusión. Resultados: la documentación revisada aportó información valiosa para determinar el estado actual de la atención de la maloclusión de estudio. Los especialistas emitieron diferentes criterios sobre el diagnóstico y tratamiento de la maloclusión de clase II. Los expertos establecieron los elementos invariables para su clasificación. Finalmente se diseñó una guía de atención para los pacientes con maloclusión de clase II con dentición permanente en crecimiento activo, valorada por los especialistas con la categoría de excelente. Conclusiones: la guía propuesta contiene elementos invariables de diagnóstico y pautas de tratamiento con el fin de lograr una atención más integral y eficiente de la maloclusión.

Foundation: Class II malocclusion has the highest prevalence among dentoskeletal disorders. Objective: to propose a care guide for patients with class II malocclusion with actively growing permanent dentition. Method: a mixed approach technological innovation research was carried out in the Clinical Sciences Department from the Stomatology Faculty of the Villa Clara Medical Sciences University, from 2020 to 2023. The studied universe was all the Orthodontic specialists in the Villa Clara province, Sancti Spíritus and Cienfuegos, from which an intentional non-probabilistic sampling was carried out by criteria and the sample was made up of 20 specialists and seven experts (higher category specialists) with criteria of inclusion. Results: The reviewed documentation provided valuable information to determine the current state of care for malocclusion under study. Specialists issued different criteria on the diagnosis and treatment of class II malocclusion. The experts established the invariable elements for their classification. Finally, a care guide was designed for patients with class II malocclusion with actively growing permanent dentition, rated by specialists as excellent. Conclusions: the proposed guide contains invariable diagnostic elements and treatment guidelines in order to achieve more comprehensive and efficient malocclusion care.

A finite element study comparing Advansync® and Twin Block in mandibular anterior repositioning.

OBJECTIVE: This study aims to utilize the finite element method (FEM) to compare the dentoalveolar and mandibular effects associated with anterior mandibular repositioning using AdvanSync® (ADV) and Twin Block (TB). METHODS: A patient with Class II skeletal malocclusion and mandibular retrognathism was selected. A TB appliance was subsequently applied. Computed Tomography (CT) scans were acquired at the beginning of treatment (T1) and 8 months later (T2). Concurrently, a numerical TB model was validated through FEM simulations, which were compared with the T2 results. The ADV appliance was virtually simulated to evaluate stress and deformation on the condyle, symphysis, first lower molar and lower central incisors. RESULTS: Both simulations demonstrated significant mandibular advancement. However, ADV led to less incisor proclination and more molar intrusion compared to TB. ADV exhibited increased stress in the lower molar area, while TB had higher stress in the lower incisor region. Stress and deformations in the condyle and mandibular symphysis were similar in both simulations, with the highest stress observed at the condylar neck and the lowest at the upper pole of the condylar head. CONCLUSIONS: Both appliances achieved similar levels of mandibular advancement, with greater proclination of the lower central incisors and more widespread distribution of stress and molar intrusion when using ADV compared to TB.

Comparative evaluation of the dentoalveolar effects of three Class II correctors: A finite element analysis study.

OBJECTIVE: To compare the stress distribution and total strain applied to the dentition, periodontal ligament (PDL) and cortical and trabecular bones by three Class II correctors using finite element analysis. DESIGN: Three-dimensional analysis of stresses and total strain of the dentition with three Class II correctors. SETTING: Computational study. METHODS: Three-dimensional finite element models of Class II elastics, the Forsus Fatigue Resistant Device (FRD) and the Carriere Motion Appliance (CMA) were constructed from a cone-beam computed tomography (CBTC) image of an orthodontic Class II patient. The distribution of stress (von Mises and principal stress) and the total strain (mm) in maxillo-mandibular dentition, PDL, cortical and trabecular bone were analysed. RESULTS: The highest von Mises yield and the maximum principal stress in the three models were found at the teeth, followed by the cortical bone, trabecular bone and PDL. The maximum stress and total deformation were located at the upper canines and lower molars in the Class II elastics and CMA models, in the upper first molars in the Forsus FRD and CMA, and in the lower first premolars in the Forsus FRD. In addition, stress was distributed in the anterior and posterior regions of the teeth, and the total deformation was found in the distal direction in the upper arch and in the mesial direction in the lower arch. CONCLUSION: The stress concentrations in the three models were located close to the active components of each appliance, producing specific patterns of stress distribution and displacement that should be taken into account when planning the type of appliance to be used for the correction of the Class II malocclusion.

Miniplaca palatina versátil: um leque de possibilidades / Versatile mini palatal plate: a range of possibilities

O palato é uma região considerada ideal para a inserção de dispositivos de ancoragem temporária (DAT's). É conhecido por possuir áreas com boa quantidade e qualidade óssea, longe das raízes dos dentes, com uma gengiva de boa qualidade, e dispor de um fácil acesso para uma correta instalação. O objetivo desse artigo é apresentar uma miniplaca palatina com mecânica originada na Coréia do Sul (BOS ­ Biocreative Orthodontic Strategy) como uma alternativa de ancoragem esquelética, por meio de um caso clínico e a demonstração de um passo a passo de sua instalação. Ela se mostra muito útil e eficiente em casos de distalização de molares superiores, mesialização ou intrusão dos mesmos. Por esta versatilidade, aqui no Brasil esta miniplaca recebe o nome de miniplaca palatina versátil (MPV). A MPV representa uma alternativa bastante eficaz em casos onde é necessário ancoragem esquelética sem incisão, se destacando em pontos como: simplicidade de instalação; estética; controle biomecânico; versatilidade e valor acessível. (AU)

The palate is a region considered ideal for the insertion of temporary anchorage devices (DAT's). It is known for having areas with good bone quantity and quality, away from the roots of the teeth, with good quality gums, and having easy access for correct installation. The objective of this article is to present a mini palatal plate with mechanics originated in South Korea (BOS ­ Biocreative Orthodontic Strategy) as an alternative for skeletal anchorage, through a clinical case and a step-by-step and the installation procedure. It proves to be very useful and efficient in cases of maxillary molar distalization, mesialization or intrusion of the same. Due to this versatility, here in Brazil this miniplate is called versatile mini-palatal plate (MPV). The MPV represents a very effective alternative in cases where skeletal anchorage without incision is required, standing out in points such as: simplicity of installation; aesthetics; biomechanical control; versatility and affordable value (AU)

Utilização do Carriere® Motion 3DTM na correção da má oclusão de Classe II / Use of the Carriere® Motion 3DTM to correct Class II malocclusion

Uma opção de tratamento para a má oclusão de Classe II de Angle é a distalização dos molares superiores, que pode ser obtida através de diferentes estratégias. O Carriere® Motion 3DTM (CM3D) é uma alternativa recente para a correção desse tipo de má oclusão e vem alcançando resultados bastante satisfatórios, por meio da distalização em bloco do segmento posterior superior, com controle tridimensional do movimento dentário. Esse aparelho apresenta um design moderno e pode ser utilizado tanto nos casos de Classe II unilaterais como bilaterais. Este trabalho tem como objetivo apresentar o caso de um paciente de 14 anos, portador de uma má oclusão de Classe II divisão 2, que foi tratado com CM3D e ancoragem inferior com arco lingual de Nance. Inicialmente foi instalado o CM3D por quatro meses para correção sagital, seguido por aparelho fixo total superior e inferior para finalização do caso. Após 24 meses de tratamento, observou-se significante melhora no sorriso e no perfil do paciente. CM3D é uma opção eficaz, prática, estética e de fácil aplicação profissional e bem aceito pelo paciente para o tratamento da má oclusão de Classe II. (AU)

A treatment option for Class II Angle occlusion is the distalization of the upper molars, which can inform through different strategies. The Carriere® Motion 3DTM (CM3D) is a recent alternative for the correction of this type of malocclusion and it has been achieving satisfactory results, utilizing block distalization of the upper posterior segment, with three-dimensional control of tooth movement. This device has a modern design and can be used in both unilateral and bilateral Class II cases. This study aims to present a case of a 14-year-old patient with Class II division 2 malocclusion, who was treated with the CM3D and lower anchorage with Nances lingual arch. Initially, it was installed a CM3D for four months for sagittal correction, followed by a total upper and lower fixed device for finalizing the case. After 24 months of detected treatment, the the patients' smile and profile significantly improved. The CM3D is a useful, practical, aesthetic, and easy to apply professional and well accepted option for the treatment of Class II malocclusion.(AU)

The efficiency of a customized distalizer with Variety SP® screws anchored on palatal miniscrews for upper molar distalization

ABSTRACT Objective: To assess the effectiveness of a customized distalizer with Variety SP® screws anchored on palatal miniscrews for upper molar distalization. Methods: Seventeen patients aged between 12.5 and 24 years underwent distalization with a customized distalizer. Lateral cephalogram and cast analysis were performed before and after distalization. Linear and angular parameters of the upper first molar, first premolar, and central incisor were assessed. Results: Distalization with the force passing near the center of resistance (CRes) of the upper first molars resulted in distal movement, with minimal distal tipping (2.8±0.45°, p<0.05). However, distalization passing occlusal to the CRes led to greater distal tipping (13.6±1.63°, p<0.05). Statistically significant spontaneous distal tipping and distal movement of the upper first premolars occurred, with a mean of 6.2±1.24° (p<0.05) and 0.68±0.34 mm (p<0.05), respectively. The positional change of the upper central incisors presented a mean of -0.23±0.1 mm (p>0.05) and 2.65±1.1° (p<0.05). Upper first molar intrusion was statistically significant, with a mean of 0.88±0.2 mm (p<0.05). Upper right and left first molars rotation towards palatal midline presented mean of 4.1±0.19° (p<0.05) and 3.4 ±0.1° (p<0.05), respectively. Additionally, the distance between upper right and left first molars increased significantly, with a mean of 2.54±0.01 mm (p<0.05). Conclusion: The study successfully demonstrated the efficiency of molar distalization without anchorage loss using a customized distalizer anchored on palatal miniscrews.

RESUMO Objetivo: Avaliar a eficácia de um distalizador personalizado com parafusos Variety SP® ancorado em mini-implantes palatinos para distalização de molares superiores. Métodos: Dezessete pacientes com idade entre 12,5 e 24 anos foram submetidos à distalização com o distalizador customizado. Análises das radiografias cefalométricas laterais e dos modelos de gesso foram realizadas antes e depois da distalização. Foram avaliados parâmetros lineares e angulares dos primeiros molares, primeiros pré-molares e incisivos centrais superiores. Resultados: A distalização com a força passando perto do centro de resistência (CRes) dos primeiros molares superiores resultou em distalização com inclinação mínima (2,8 ± 0,45°, p<0,05). Entretanto, a distalização passando oclusal ao CRes levou a uma maior inclinação para distal (13,6 ± 1,63°, p<0,05). Ocorreu inclinação distal espontânea e movimento distal estatisticamente significativo dos primeiros pré-molares superiores, com média de 6,2 ± 1,24° (p<0,05) e 0,68 ± 0,34 mm (p<0,05), respectivamente. A mudança de posicionamento dos incisivos centrais superiores apresentou média de -0,23 ± 0,1 mm (p>0,05) e 2,65 ± 1,1° (p<0,05). A intrusão dos primeiros molares superiores foi estatisticamente significativa, com média de 0,88 ± 0,2 mm (p<0,05). A rotação dos primeiros molares superiores direito e esquerdo em direção à linha média palatina apresentou média de 4,1 ± 0,19° (p<0,05) e 3,4 ± 0,1° (p<0,05), respectivamente. Além disso, a distância entre os primeiros molares superiores direito e esquerdo aumentou significativamente, com média de 2,54 ± 0,01 mm (p<0,05). Conclusão: O estudo demonstrou com sucesso a eficiência da distalização de molares sem perda de ancoragem utilizando um distalizador customizado ancorado em mini-implantes palatinos.

Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor Binding Protein-3 - Biomarkers for Skeletal Maturity Assessment in Class II Malocclusion

ABSTRACT Objective: To estimate the serum levels of non-radiologic biomarkers, Insulin-like Growth Factor-1 (IGF-1), and Insulin-like Growth Factor Binding Protein-3 (IGFBP-3) to potentially identify the pubertal growth spurt in skeletal Class II malocclusion subjects. Material and Methods: Eighty subjects (M-38, F-42) with skeletal Class II malocclusion in the age range of 11-18 years were recruited for the cross-sectional study. Human serum IGF-1 and IGFBP-3 were quantitatively assessed by enzyme-linked immunosorbent assay, and the cervical stage (CS) was evaluated from a lateral cephalogram. Results: Gender-wise comparison of the mean serum IGF-1 levels revealed that the initial peak was detected at CS2 in both genders, [males (87.87 ng/mL), females (78.49 ng/mL)]. However, there was a cognizable difference in the second peak of the mean serum IGF-1 levels between males (CS5, 68.58 ng/mL) and females (CS4, 74.63 ng/mL). Mean IGFBP-3 serum levels in male subjects were high in CS4 (47.24 ng/mL) with a further spike in CS6 (50.54 ng/mL), and in female subjects, it was found to be highest in CS3 (51.95 ng/mL) and then in CS5 (49.68 ng/mL). Conclusion: Mean IGF-1 levels exhibited both sexes' prepubertal and late pubertal spikes. Mean IGFBP-3 levels revealed a pubertal and a late pubertal spike in both sexes, with an earlier growth trend observed specific to females compared to males.

Finite element analysis of two skeletally anchored maxillary molar distalisation methods.

OBJECTIVE: To compare two methods of maxillary molar distalisation with skeletal anchorage using finite element analysis (FEA). METHODS: Two digitised models were created: the miniscrew-anchored distaliser, which consisted of a distalisation method anchored in a buccal miniscrew between the first molar and second premolar (Model 1), and the miniscrew-anchored palatal appliance, which consisted of a distalisation method anchored in a miniscrew on the anterior region of the palate (Model 2). FEA was used to simulate both methods, assessing teeth displacements and stress concentration. RESULTS: The miniscrew-anchored distaliser showed greater buccal than distal displacement of the first molar, while the opposite was observed in the miniscrew-anchored palatal appliance. The second molar responded similarly in the transverse and anteroposterior perspectives with both appliances. Greater displacements were observed at crown level than in apical regions. Greater stress concentration was observed at the buccal and cervical regions of the crown in the miniscrew-anchored distaliser and the palatal and cervical regions in the palatal appliance. The stress progressively spread in the buccal side of the alveolar bone for the miniscrew-anchored distaliser and in the palatal root and alveolar bone for the palatal appliance. CONCLUSION: FEA assumes that both appliances would promote maxillary molar distalisation. A skeletally anchored palatal distalisation force seems to provide a greater molar bodily movement with less undesirable effects. Greater stress is expected at the crown and cervical regions during distalisation, and the stress concentration in the roots and alveolar bone depends directly on the region the force was applied.

Biomechanical and chemical behavior of various bioactive materials in class II restorations / Desempeño biomecánico y químico de diferentes materiales bioactivos en restauraciones clase II

The aim of this in vitro study was to evaluate the biomechanical and chemical behavior of various bioactive materials in class II MOD restorations. Forty- eight standardized class II MOD cavities were prepared in sound extracted human molar teeth. The specimens were divided into four groups according to the restorative material: Group 1 (Filtek™ Z350 XT), Group 2 (Biodentine™ as a liner, and then restored with Filtek™ Z350 XT), Group 3 (Cention N™), and Group 4 (Activa™ Bioactive-Restorative). The samples were tested for fracture resistance by subjecting them to a compressive load in a Universal testing Machine. The failure modes of each specimen were evaluated. The alkalinizing potential and calcium ion release of the materials were measured. SEM-EDAX analyses were also performed for all materials. Data were analyzed using ANOVA and post hoc Tukey test (p<0.05). Group 1 showed the higher fracture resistance (p<0.05). Group 3 had greater fracture resistance values but no statistical difference from Group 4. Biodentine™ showed greater biomineralization potential. Class II MOD restorations of Group 1 displayed the higher fracture resistance; however, it was highly associated with catastrophic failure. Conversely, Biodentine™ presented a more significant bioactivity potential, and its use, as in Group 2, promoted the most favorable failure mode.

El objetivo de este estudio, in vitro, fue evaluar el desempeño biomecánico y químico de varios materiales bioactivos en restauraciones clase II MOD. Se prepararon cuarenta y ocho cavidades clase II MOD estandarizadas en dientes molares humanos extraídos. Las muestras se dividieron en cuatro grupos según el material de restauración: Grupo 1 (Filtek™ Z350 XT), Grupo 2 (Biodentine™ como base y luego restaurado con Filtek™ Z350 XT), Grupo 3 (Cention N™) y Grupo 4 (Activa™ Bioactivo-Reparador). La prueba de resistencia a la fractura fue realizada en una máquina de ensayo universal Instron. Se evaluaron los modos de falla de cada espécimen. Se midió el pH y la liberación de iones de calcio de los materiales. Se realizaron análisis SEM-EDAX. Los datos se analizaron mediante ANOVA y la prueba post hoc de Tukey (p<0,05). El grupo 1 mostró la mayor resistencia a la fractura (p<0,05). El Grupo 3 tuvo mayores valores de resistencia a la fractura que el Grupo 4, pero sin diferencia estadística. Biodentine™ mostró un mayor potencial de biomineralización. Las restauraciones Clase II MOD del Grupo 1 mostraron la mayor resistencia a la fractura; sin embargo, estuvo altamente asociado con fallas irreparables. Por el contrario, Biodentine™ presentó un potencial de bioactividad más significativo y su uso, como en el Grupo 2, promovió el modo de falla más favorable.

Connection between MHC class II binding and aggregation propensity: The antigenic peptide 10 of Paracoccidioides brasiliensis as a benchmark study.

The aggregation of epitopes that are also able to bind major histocompatibility complex (MHC) alleles raises questions around the potential connection between the formation of epitope aggregates and their affinities to MHC receptors. We first performed a general bioinformatic assessment over a public dataset of MHC class II epitopes, finding that higher experimental binding correlates with higher aggregation-propensity predictors. We then focused on the case of P10, an epitope used as a vaccine candidate against Paracoccidioides brasiliensis that aggregates into amyloid fibrils. We used a computational protocol to design variants of the P10 epitope to study the connection between the binding stabilities towards human MHC class II alleles and their aggregation propensities. The binding of the designed variants was tested experimentally, as well as their aggregation capacity. High-affinity MHC class II binders in vitro were more disposed to aggregate forming amyloid fibrils capable of binding Thioflavin T and congo red, while low affinity MHC class II binders remained soluble or formed rare amorphous aggregates. This study shows a possible connection between the aggregation propensity of an epitope and its affinity for the MHC class II cleft.

Inhibition of ANGPT2 activates autophagy during hypertrophic scar formation via PI3K/AKT/mTOR pathway

Abstract Background Hypertrophic scar (HS), a fibroproliferative disorder caused by aberrant wound healing following skin injuries such as burns, lacerations and surgery, is characterized by invasive proliferation of fibroblasts and excessive extracellular matrix (ECM) accumulation. The dysregulation of autophagy is the pathological basis of HS formation. Previously, angiopoietin-2 (ANGPT2) was found to be overexpressed in HS fibroblasts (HSFs) compared with normal skin fibroblasts. However, whether ANGPT2 participates in the process of HS formation and the potential molecular mechanisms are not clear. Objective This study is intended to figure out the role of ANGPT2 and ANGPT2-mediated autophagy during the development of HS. Methods RT-qPCR was used to detect ANGPT2 expression in HS tissues and HSFs. HSFs were transfected with sh-ANGPT2 to knock down ANGPT2 expression and then treated with MHT1485, the mTOR agonist. The effects of sh-ANGPT2 or MHT1485 on the proliferation, migration, autophagy and ECM accumulation of HSFs were evaluated by CCK-8 assay, Transwell assay and western blotting. The expression of PI3K/Akt/mTOR pathway-related molecules (p-PI3K, p-Akt and p-mTOR) was assessed by western blotting. Results ANGPT2 expression was markedly upregulated in HS tissues and HSFs. ANGPT2 knockdown decreased the expression of p-PI3K, p-Akt and p-mTOR. ANGPT2 knockdown activated autophagy and inhibited the proliferation, migration, and ECM accumulation of HSFs. Additionally, the treatment of MHT1485, the mTOR agonist, on ANGPT2-downregulated HSFs, partially reversed the influence of ANGPT2 knockdown on HSFs. Study limitations The study lacks the establishment of more stable in vivo animal models of HS for investigating the effects of ANGPT2 on HS formation in experimental animals. Conclusions ANGPT2 downregulation represses growth, migration, and ECM accumulation of HSFs via autophagy activation by suppressing the PI3K/Akt/mTOR pathway. Our study provides a novel potential therapeutic target for HS.

Evaluation of the stability of treatment with Herbst appliance associated with Hyrax expander and fixed orthodontics through counterpart analysis: A longitudinal follow-up study.

OBJECTIVE: The aim of this study was to evaluate the stability of treatment with a Herbst appliance associated with Hyrax expander (Stage I), followed by fixed appliances (Stage II) and follow-up for an average of 4 years after Stage II, on dentoskeletal facial structures. METHODS: This study involved 50 adolescents with Angle Class II division 1 malocclusion associated with mandibular retrognathism: Treated Group (TG-25) and Control Group (CG-25). Lateral cephalometric radiographs were taken: T1, immediately before Stage I (TG) or at the beginning of the follow-up period (CG); T2, at the end of Stage I (TG) or the follow-up period (CG); T3, at the end of Stage II (TG); and T4, on average, 4 years after Stage II (TG). Enlow's counterpart analysis and some cephalometric measurements were evaluated. Parametric and non-parametric tests were used (P ≤ 0.05). RESULTS: The ramus alignment variables (P < 0.001), SNB (0.040), ANB (<0.001), 1.PP (P = 0.015), 1.MP (P < 0.001), ms/RLp (P < 0.001), mi/RLp (P < 0.001) and S-LS (P = 0.005) showed differences between TG and CG from T1 to T2. Longitudinally, there were differences in ramus alignment, P = 0.003, T1 > T2 < T3 = T4; SNB, P = 0.016, T1 < T2 = T3 = T4; ANB, P < 0.001, T1 > T2 = T3 = T4; 1.MP, P < 0.001, T1 < T2 = T3 = T4; ms/RLp, P = 0.002, T1 = T2 < T3 = T4; mi/RLp, P < 0.001, T1 < T2 = T3 = T4; S-LS, P < 0.001, T1 > T2 = T3 = T4 and S-LI, P = 0.003, T1 = T2 = T3 > T4. CONCLUSION: The nasomaxillary complex (MCF/PM alignment) tended to a retrusive effect to compensate the degree of mandibular retrusion. The protrusive effect of the lower facial third was evident after the Herbst stage and did not remain stable in the follow-up. The dentoalveolar compensation and improvement in facial profile remained stable.

Inhibition of ANGPT2 activates autophagy during hypertrophic scar formation via PI3K/AKT/mTOR pathway.

BACKGROUND: Hypertrophic scar (HS), a fibroproliferative disorder caused by aberrant wound healing following skin injuries such as burns, lacerations and surgery, is characterized by invasive proliferation of fibroblasts and excessive extracellular matrix (ECM) accumulation. The dysregulation of autophagy is the pathological basis of HS formation. Previously, angiopoietin-2 (ANGPT2) was found to be overexpressed in HS fibroblasts (HSFs) compared with normal skin fibroblasts. However, whether ANGPT2 participates in the process of HS formation and the potential molecular mechanisms are not clear. OBJECTIVE: This study is intended to figure out the role of ANGPT2 and ANGPT2-mediated autophagy during the development of HS. METHODS: RT-qPCR was used to detect ANGPT2 expression in HS tissues and HSFs. HSFs were transfected with sh-ANGPT2 to knock down ANGPT2 expression and then treated with MHT1485, the mTOR agonist. The effects of sh-ANGPT2 or MHT1485 on the proliferation, migration, autophagy and ECM accumulation of HSFs were evaluated by CCK-8 assay, Transwell assay and western blotting. The expression of PI3K/Akt/mTOR pathway-related molecules (p-PI3K, p-Akt and p-mTOR) was assessed by western blotting. RESULTS: ANGPT2 expression was markedly upregulated in HS tissues and HSFs. ANGPT2 knockdown decreased the expression of p-PI3K, p-Akt and p-mTOR. ANGPT2 knockdown activated autophagy and inhibited the proliferation, migration, and ECM accumulation of HSFs. Additionally, the treatment of MHT1485, the mTOR agonist, on ANGPT2-downregulated HSFs, partially reversed the influence of ANGPT2 knockdown on HSFs. STUDY LIMITATIONS: The study lacks the establishment of more stable in vivo animal models of HS for investigating the effects of ANGPT2 on HS formation in experimental animals. CONCLUSIONS: ANGPT2 downregulation represses growth, migration, and ECM accumulation of HSFs via autophagy activation by suppressing the PI3K/Akt/mTOR pathway. Our study provides a novel potential therapeutic target for HS.

Tratamento da Classe II com distalização sequencial dos dentes superiores utilizando alinhadores Invisalign / Class II treatment with sequential distalization of upper teeth using Invisalign aligners

Resumo A utilização de alinhadores no tratamento ortodôntico é uma realidade cada vez mais presente na rotina do ortodontista, especialmente em pacientes adultos que buscam alternativas mais confortáveis e estéticas para os aparelhos fixos convencionais. O tratamento da Classe II com alinhadores representa um grande desafio na Ortodontia atual, uma vez que o planejamento do caso, os princípios mecânicos empregados e os resultados esperados podem variar em relação às mecânicas tradicionais. Considerando a importância e a recorrência do tema na atualidade, o objetivo deste artigo foi relatar o caso de uma paciente adulta com má oclusão de Classe II tratada com a distalização sequencial dos dentes superiores utilizando alinhadores Invisalign, bem como discutir a prescrição utilizada e os resultados do tratamento, esclarecendo os ortodontistas sobre as potenciais vantagens e desvantagens desta modalidade de tratamento (AU)

Abstract The use of aligners in orthodontic treatment is an increasingly reality in the routine of orthodontists, especially in adult patients who seek more comfortable and aesthetic alternatives to conventional fixed appliances. The treatment of Class II with aligners represents a major challenge in current Orthodontics, since case planning, the mechanical principles employed, and the expected results may vary in relation to traditional mechanics. Considering the current importance and recurrence of the topic, the objective of this article was to report the case of an adult patient with Class II malocclusion treated with sequential distalization of the upper teeth using Invisalign, as well as to discuss the prescription employed and the results of the treatment, clarifying orthodontists about the potential advantages and disadvantages of this treatment modality (AU)

Tratamento de má oclusão de Classe II por meio de distalização sequencial posterior com alinhadores ortodônticos ClearCorrect / Treatment of Class II malocclusion through upper distalization with ClearCorrect aligners

Resumo A segunda mais prevalente má oclusão de Angle na população é a de Classe II. Dentre as abordagens de tratamento consideradas pelos profissionais, está a distalização dos dentes posteriores superiores ou exodontias. A distalização, dependendo do nível de complexidade do caso, pode ser desafiadora e necessitar de mecânicas auxiliares para realizar movimentos translacionais e não somente de inclinação coronária. A escolha da abordagem para esses tratamentos depende diretamente da colaboração do paciente, severidade da má oclusão, caracterização da Classe II, idade do paciente e habilidade técnica do profissional responsável. Com o aumento da procura por tratamentos ortodônticos mais estéticos, os alinhadores ortodônticos têm sido frequentemente uma alternativa aos pacientes. Dependendo da severidade da má oclusão, pode-se associar aos auxiliares comumente utilizados na Ortodontia convencional fixa. Portanto, o objetivo deste trabalho é apresentar a resolução de uma má oclusão de Classe II, divisão 1, com impactação do canino superior direito e desvio de linha média superior para a direita tratada com alinhadores ortodônticos ClearCorrect. (AU)

Abstract The second more prevalent Angle's malocclusion on the population is Class II. Among the continuously considered treatment approaches by clinicians, is the distalization of upper teeth or extractions. The distalizations, depending on the case's complexity, can be challenging and need auxiliary mechanics to perform translation movements and not just crown tipping. The choose of the appliance for these treatments relies directly on the option/collaboration of the patient, malocclusion severeness, patient's age, and technical ability of the main professional taking care of the case. With the crescent search for more aesthetic orthodontic treatments, the clear aligners have been frequently looked for by the patients as an alternative and, regularly related to auxiliaries commonly associated to the conventional fixed appliances. Regarding this context, the objective of this study is to present the case report of a patient with Class II, division 1, upper midline deviation to the right and unerupted upper right canine through the ClearCorrect aligners' system. (AU)

Utilização do PowerScope para potencializar o tratamento da Classe II com Invisalign / Use of PowerScope to enhance Class II treatment with Invisalign

Resumo Corrigir a relação molar de Classe II em um paciente adulto não é uma tarefa fácil. E com os alinhadores ortodônticos isso torna-se ainda mais difícil. Os alinhadores têm dificuldade na sua correção, principalmente porque sua eficácia depende do uso de elásticos e, muitas vezes, os pacientes não colaboram o suficiente. Há casos em que o paciente deveria ser tratado com cirurgia ortognática, mas o paciente não aceita ser submetido a ela. Então, podemos tratá-lo de forma compensatória utilizando um propulsor mandibular, como o PowerScope. Este artigo descreve três maneiras de incorporar o PowerScope no tratamento da Classe II com o Invisalign. São descritos os desenhos híbrido, simplificado e complexo, cada um ilustrado por um caso clínico. Desta forma, pode-se concluir que o PowerScope pode ser um grande aliado no tratamento de casos complexos de Classe II junto aos alinhadores Invisalign. A abordagem é muito promissora, mas ainda necessita de alguns ajustes (AU)

Abstract Correcting a Class II molar relationship in an adult patient is not an easy task. And with orthodontic aligners this becomes even more difficult. Aligners have difficulties in Class II correction, mainly because their effectiveness depends on the use of elastics, and patients often do not cooperate enough. There are cases in which patients should be treated with orthognathic surgery, but they do not accept the surgery. So, we can treat them compensatory using a Class II corrector, like the PowerScope. This article describes three ways to incorporate PowerScope into Class II treatment with Invisalign. The designs are described as hybrid, simplified, complex and each one is illustrated by a clinical case. In this way, it can be concluded that PowerScope can be a great ally in the treatment of complex Class II cases with Invisalign aligners. The approach is very promising, but still needs some adjustments (AU)

O distalizador Carriere como coadjuvante no tratamento da má oclusão de Classe II, Divisão 1 / The Carriere distalizer as an adjunct in the treatment of Class II, Division 1 malocclusion

Resumo O objetivo deste relato de caso é descrever o tratamento ortodôntico realizado em uma paciente de 15 anos, sexo feminino, com má oclusão Classe II, Divisão 1, com aparelho Carriere Motion 3D (ACM), seguido de aparelhos fixos completos. O exame intraoral mostrou uma má oclusão de Classe II, Divisão 1, com overbite de 1 mm e overjet de 3,5 mm. O arco superior com morfologia triangular e o mandibular parabólico, além da ausência da coroa clínica do primeiro molar inferior direito. O tratamento iniciou com a utilização simultânea do ACM e de uma contenção Essix para ancoragem do arco mandibular. Nesse período, elásticos de Classe II foram empregados durante 5 meses, obtendo-se a relação molar e canino de Classe I. Posteriormente, bráquetes metálicos MBT .022" foram utilizados em ambas as arcadas. Os resultados demonstraram estabilidade ao longo de um período de dois anos de acompanhamento, com satisfação da paciente (AU)

Abstract The aim of this case report is to describe the orthodontic treatment performed on a 15-year-old female patient, with Class II, Division 1 malocclusion, using the Carriere Motion 3D appliance (ACM) followed by full fixed appliances. The intraoral examination showed a Class II, Division 1 malocclusion, with 1 mm overbite and 3.5 mm overjet. The maxillary arch had a triangular morphology and the mandibular arch was parabolic, in addition to the absence of the clinical crown of the right mandibular first molar. Treatment began with the simultaneous use of ACM and an Essix retainer for anchoring the mandibular arch. During this period, Class II elastics were applied for 5 months, achieving Class I molar and canine relation. Subsequently, MBT .022" metal brackets were used on both arches. The results demonstrated stability over a two-year follow-up period, with patient satisfaction (AU)

Tratamento de uma má oclusão de Classe II com propulsor mandibular Forsus associado à tração de canino inferior impactado devido ao odontoma ­ relato de caso / Treatment of a Class II malocclusion with Forsus appliance associated with traction of an impacted mandibular canine due to odontoma ­ a case report

Resumo O objetivo deste caso clínico foi relatar o tratamento de uma paciente portadora de má oclusão de Classe II com aparelho de propulsão mandibular fixo Forsus associado à tração de canino inferior retido devido a um odontoma. Após exame clínico e radiográfico, foi detectada a retenção prolongada do canino inferior decíduo direito devido à presença desse odontoma. O exame clínico também mostrou que a paciente apresentava uma má oclusão de Classe II. Após a extração do dente decíduo e excisão do odontoma, foi colado um acessório no canino retido e instalado o aparelho fixo. Inicialmente, foi planejada a instalação de um APM devido ao seu baixo custo, no entanto, a paciente não se adaptou e foi instalado um Forsus. Ao final de 48 meses, o canino inferior direito alcançou o plano oclusal e a má oclusão de Classe II foi corrigida. Após 2 anos da finalização do tratamento, os resultados obtidos se mostraram estáveis. O objetivo foi avaliar os efeitos dentários e tecidos moles no tratamento da má oclusão de Classe II leve a moderada de uma paciente no final de crescimento puberal, analisando o antes e depois da face no desenvolvimento dentofacial e os efeitos do aparelho de protração. Concluiu-se que o propulsor mandibular associado ao aparelho fixo corrigiu a má oclusão de Classe II basicamente através da protrusão dentoalveolar do arco inferior. (AU)

Abstract The objective of this case report is to present the treatment of a patient with Class II malocclusion with a Forsus fixed appliance associated with the traction of an impacted mandibular canine due to an odontoma. After clinical and radiographic examination, prolonged right primary mandibular canine retention was detected. In addition, the mandibular canine was impacted due to the presence of this odontoma. Clinical analysis also showed that the patient had a Class II malocclusion. After the extraction of the primary canine and excision of the odontoma, an orthodontic accessory was bonded to the impacted canine, and the fixed appliance was placed. Initially, an APM (mandibular protractor appliance) was planned due to its low cost. However, the patient did not cooperate with its use, and a Forsus was placed. At the end of 48 months, the right mandibular canine reached the occlusal plane, and the Class II malocclusion was corrected. (AU)