Nasal Reconstruction Using Rib Grafting with Cold Light Source Technology.

BACKGROUND: Although costal cartilage is a reliable source of cartilage for rhinoplasty and provides a strong scaffold for total nasal reconstruction, traditional collection techniques may cause complications at the donor site. In this paper, we report a simple and safe technique for harvesting full-length costal cartilage and its application in total nasal reconstruction. METHODS: From May 2018 to December 2020, 24 patients with nasal defects, including 16 females and eight males, received nasal reconstruction in the Rhinoplasty and Repair Center of the Plastic Surgery Hospital of the Chinese Academy of Medical Sciences. Clinical outcomes were evaluated during the postoperative stay at the hospital and at the 6-30-month follow-up. RESULTS: The operative time of cartilage harvesting ranged from 30 to 60 min. The patients could walk freely one day after surgery. The average ± standard deviation of Visual Analog Scale scores for pain at the harvested site were 2.583 ± 0.717 (at rest) and 4.750 ± 0.794 (during coughing) 6 h after surgery. We observed no complications (e.g., pleural perforation, pneumothorax, or massive bleeding) due to rib grafts in any patients. During the 6-30 months of follow-up, all patients had complete healing of both donor and recipient sites. The surgical results were rated as satisfactory or good by the patients and surgeons. CONCLUSIONS: This new cold light source-assisted costal cartilage harvest technique allows full-length costal cartilage to be obtained for total nasal reconstruction, with minimal donor site complications, short operation time, fast postoperative recovery, and high satisfaction among patients and surgeons. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Efficient Cascade Resonance Energy Transfer in Dynamic Nanoassembly for Intensive and Long-Lasting Multicolor Chemiluminescence.

Light emission induced by chemical reactions, known as chemiluminescence (CL), has been widely used for bioassays, biosensors, imaging, and illumination applications. Most known CL systems exhibit flash-type single-color light emissions, which limit their applications. Long-lasting multicolor CL in aqueous solutions is highly desirable, especially for biological applications, but remains a challenge. Herein, we report a simple strategy of achieving highly efficient cascade Förster resonance energy transfer (FRET) in the dynamic nanoassembly of ß-cyclodextrin (ß-CD), CL reagents, and fluorophores in aqueous solution, which emits intensive multicolor CL with adjustable wavelength within 410-610 nm. ß-CD can bind CL reagents and fluorophores to form a dynamic nanoassembly. These nanoassemblies can bring the included luminescent intermediate and fluorophores into close proximity and proper alignment, which should greatly enhance the FRET efficiency between luminescent intermediate and fluorophores. Indeed, the cascade FRET efficiency in this supramolecular nanoassembly reaches up to 92%, which is comparable with the cascade FRET systems based on covalently linked donors and acceptors. By using hydroxypropyl methylcellulose as the thickener to slow the diffusion (to elongate the CL emission), and using Ca(OH)2 solid (a low solubility strong base) as buffer to maintain the pH in the optimal range for the CL reaction, this nanoassembly system has been further developed to achieve slow-diffusion-controlled catalytic CL reactions, which enables long-lasting multicolor CL in aqueous solution that is visible to naked eyes and lasts for more than 20 h. The multicolor CL systems can be used to prepare transformable two-dimensional multicolor codes for encryption application.

The Effect of Endoscopic Tympanoplasty on Cochlear Function.

OBJECTIVES: The aim of this prospective clinical study was to measure the audiologic outcomes of the patients that underwent endoscopic transcanal cartilage tympanoplasty, and to investigate the effects on cochlear function. METHODS: Thirty-three patients (33 ears) who were diagnosed with noncomplicated chronic otitis media and underwent endoscopic transcanal cartilage tympanoplasty technique were included. Pre- and postoperative first month distortion product otoacoustic emission (DPOAE) signal-to-noise ratio (SNR), bone conduction hearing levels and air bone gap (ABG) values were measured and total endoscope usage time was noted. RESULTS: Preoperative median SNR measurements at 0.5, 1, 2, and 4 kHz were 6 dB (1-11; interquartile range [IQR], 1), 6 dB (4-20; IQR, 1), 7 dB (3-26; IQR, 5) and 5.50 dB (0-9; IQR, 3), respectively. Postoperative median SNR measurements at 0.5, 1, 2, and 4 kHz were 6 dB (3-9; IQR, 1), 6 dB (2-21; IQR, 3), 7 dB (2-20; IQR, 3), and 6 dB (0-10; IQR, 7), respectively. Regarding the DPOAE measurements, there was no statistically significant difference between the SNR values of all given frequencies (P>0.05). Regarding the pure tone audiometry (PTA) measurements, bone conduction was significantly better at 0.5 and 1 kHz, postoperatively (P<0.05) and there was statistically significant difference at 2 and 4 kHz (P>0.05). Additionally, no statistically significant correlation was found between the SNR and PTA measurements and the endoscope usage time (P>0.05). CONCLUSION: We suggested that cochlear functions and sensorineural hearing remained stable after endoscopic transcanal cartilage tympanoplasty and cold light source doesn't cause significant adverse effects cochlear functions.

The Effect of Endoscopic Tympanoplasty on Cochlear Function

OBJECTIVES: The aim of this prospective clinical study was to measure the audiologic outcomes of the patients that underwent endoscopic transcanal cartilage tympanoplasty, and to investigate the effects on cochlear function. METHODS: Thirty-three patients (33 ears) who were diagnosed with noncomplicated chronic otitis media and underwent endoscopic transcanal cartilage tympanoplasty technique were included. Pre- and postoperative first month distortion product otoacoustic emission (DPOAE) signal-to-noise ratio (SNR), bone conduction hearing levels and air bone gap (ABG) values were measured and total endoscope usage time was noted. RESULTS: Preoperative median SNR measurements at 0.5, 1, 2, and 4 kHz were 6 dB (1–11; interquartile range [IQR], 1), 6 dB (4–20; IQR, 1), 7 dB (3–26; IQR, 5) and 5.50 dB (0–9; IQR, 3), respectively. Postoperative median SNR measurements at 0.5, 1, 2, and 4 kHz were 6 dB (3–9; IQR, 1), 6 dB (2–21; IQR, 3), 7 dB (2–20; IQR, 3), and 6 dB (0–10; IQR, 7), respectively. Regarding the DPOAE measurements, there was no statistically significant difference between the SNR values of all given frequencies (P>0.05). Regarding the pure tone audiometry (PTA) measurements, bone conduction was significantly better at 0.5 and 1 kHz, postoperatively (P 0.05). Additionally, no statistically significant correlation was found between the SNR and PTA measurements and the endoscope usage time (P>0.05). CONCLUSION: We suggested that cochlear functions and sensorineural hearing remained stable after endoscopic transcanal cartilage tympanoplasty and cold light source doesn’t cause significant adverse effects cochlear functions.

Research on development of automatic color LED medical cold light source / 中国医学装备

According to the clinical needs, to meet the different requirements of users,to provide a kind of energy-saving, environmental,Protection, safety,reliable automatic color LED medical cold light source; using LED as light source medical cold light source, LED used red, yellow, green The three primary colors, with the three drive control; light intensity using the total luminosity regulation combined with red, yellow, green three primary color Festival; automatic regulation, tracking and artificial regulation combined. According to the camera, display and individual different regulating the best color,taking LED as the light source, using the total light intensity and color separation light (red light, yellow light, green light intensity detection), and can realize thetracking, to develop the automatic tracking set color and intensity automatic colorLED medical cold light source. Conclusion automatic color LED medical cold light source of safe use, reliable performance, energy saving and environmental protection; the different needs can be suitable for different users; and can realize automatic control and manual adjustment of light intensity and light intensity of the total.

Surgical treatment of calcified lumbar disc protrusion through a limited-incision under cold light source / 中国微创外科杂志

Objective To investigate curative outcomes of the limited-incision surgery under cold light source for treating calcified lumbar disc protrusion. Methods A total of 36 patients with calcified lumbar disc protrusion underwent limited-incision surgery under cold light visualization from January 2000 to June 2005. The patients were maintained at a prone position. The operation was carried out under local anesthesia. A longitudinal skin incision around 3 cm in length was made. After the inferior border of the vertebral plate and the intervertebral space were exposed, a lamina retractor was inserted above the superior border of the articular process. Then a fenestration and a discectomy were performed under cold light visulization. Results The operative time was 75.3?3.5 min and the intraoperative blood loss was 100.6?5.5 ml. Cerebrospinal fluid leakage occurred in 3 patients. All the 36 patients were followed for 6~60 months (mean, 27 months). Symptoms of low back pain, lower limb pain, and intermittent limping disappeared completely in all the 36 patients. According to the Nakai standard, the rate of excellent or good results was 94.4% ( 34/36 ). Conclusions Limited-incision surgery under cold light source for the treatment of calcified lumbar disc protrusion offers advantages of simplicity of performance, minimal invasion, and satisfactory outcomes.