ABSTRACT
A separation technology of catalpol from Rehmannia with macroporpus adsorbent resins was investigated. The content of catalpol in the extract was determined by high performance liquid chromatography (HPLC). Nine different kinds of macroporous adsorbent resins were studied on the static capacity of adsorption and desorption, and D101 resin was best for the separation of the extraction solution of Rehmannia. The results showed that D101 resin had the highest static adsorption capacity of 69.2mg/g dry resin and its isotherm curve can be well described by Langmuir and Freudlich equation. The 5% ethanol elution on removal of the solvent under reduced pressure provided a brown powder, which was subjected to an open column chromatography on silica gel eluted with a CHCl3–MeOH gradient. The fraction eluted with CHCl3-MeOH (8∶2) was identified as catalpol and the purity of the compound was more than 90% purity by HPLC analysis. The yield of this separation technology was 6%.
ABSTRACT
OBJECTIVE: To determine the content of Icariin in Yangqiang baoshen pills by column chromatography-HPLC. METHODS: The chromatographic column was octadecylsilane chemically bonded silica(200 mm?4.6 mm, 10 ?m) with mobile phase consisted of 0.075 mol?L-1 phosphoric acid -B cyanide (73∶27) at a detection wavelength of 270 nm. RESULTS: The linear range of Icariin was 10-18 ?g?mL-1(r=0.999 5) and the average recovery was 94.35%(RSD=1.12%,n=6).CONCLUSION: This method is simple, accurate, reproducibility and applicable for the quality control of Yangqiang baoshen pill.
ABSTRACT
OBJECTIVE:To establish quality control method for rupining strapping.METHODS:The qualitation of Herba Epimedii,Rhizoma Bolbostemmae and fructus toosendan in rupining strapping was conducted by TLC.The principal drug-epimedium herb was determined by column chromatography-HPLC.RESULTS:Herba Epimedii,Rhizoma Bolbostemmae and fructus toosendan could all be identified in TLC.Good linear correlation of icariin was observed when the sample size was0.428?g～2.568?g(r=0.9998).The average recovery was94.57%(RSD=1.43%).CONCLUSION:The method is sensi-tive,accurate,and reproducible.It can be used for the quality control of rupining stripping.
ABSTRACT
OBJECTIVE:To establish a method for determining the content of icariin in Ruzenning tablets.METHODS:Column chromatography-HPLC was adopted.C 18 column was used with acetonitrile-water(30∶70)as mobile phase and the detection wavelength was set at270nm.RESULTS:The linear range was10?g～18?g for the icariin(Y=0.9995).The average recovery was94.6%,RSD=1.95%(n=6).CONCLUSION:The method is simple and accurate with a good reproducibility and can be used as a quantitative analysis method for this preparation.
