ABSTRACT
Objective To study the mechanism of Comus officinalis glucosides treatment of IgA nephropathy.Methods Fifty SD rats were randomly divided into the control group,the IgAN model group,the Dexamethasone group,the Comus officinalis glucosides in the high-dose group and in the low-dose group.The IgA nephropathy rat models were established by feeding bovine serum albumin (BSA),subcutaneous injection of carbon tetrachloride (CCl4),castor oil and injecting tail vein with lipopolysaccharide (LPS).After 10 weeks of administration,the urine red blood cell count,UTP in each phase were observed after the rat models were successfully established.Then Dexamethasone group and Comus officinalis total glucoside group were given medicine for 8 weeks,and urine or serum content of interleukin-18 (IL-18),transforming growth factor-β1 (TGF-β1) in different groups of rats and pathological changes of kidneys were detected.Results Compared with the IgAN model group,24 h-UTP of each group decreased and Comus officinalis glucosides in high-dose group decreased extraordinary [(8.8 ± 3.2) mg/24 h vs.(35.2 ± 5.6) mg/24 h,t =10.511,P =0.000].Compared with the IgAN model group,urine red blood cell count decreased significantly in the high-dose group of Comus officinalis glucosides[(148.2 ±41.8) 个/μL vs.(683.8 ±24.6) 个/μL,t =10.909,P =0.000];but urine red blood cell count in the Dexamethasone group declined slightly,but there was no statistically significant difference(t =3.014,P =0.061).Compared with the control group,IL-18,TGF-β1,and rat urine or serum of IgAN model group was significantly elevated (all P ＜ 0.05).Compared with IgAN model group,IL-18,TGF-β1 of the high-dose group of Comus officinalis glucosides in rats urine or serum decreased significantly(all P ＜ 0.05).The effect of high-dose group of Comus officinalis glucosides on the pathological changes in kidney tissues was the most significant.Conclusions The mechanism of Comus officinalis glucosides may postpone renal lesion that can be associated with the down-regulation of the expression of IL-18,TGF-β1 and the inhibition of the deposition of IgA in the renal tissue.Comus officinalis glucosides can inhibit the proliferation of mesangial cells,tubular atrophy and interstitial fibrosis in IgAN rats,which has a certain curative effect on IgAN rats.
