Plants of Conservation Interest in a Protected Area: A Case Study of the Gran Sasso and Monti Della Laga National Park (Central Italy).

The National Park of Gran Sasso and Monti della Laga (PNGSL) is located in Central Italy and covers an area of 143.311 ha across three administrative regions (Abruzzo, Marche, and Lazio). It is the protected area hosting the highest number of vascular plants in both Europe and the Mediterranean basin. The plan of the park recognizes the need to establish a list of plants of conservation interest to prioritize for protection. The aim of this study is to identify plants (vascular and bryophytes) for inclusion on a protection list, taking into account their phytogeographic importance as well as the threat of extinction, and subsequently propose an original categorization (protection classes) suggesting specific conservation actions and measures. We used original criteria to select plants of conservation interest among the 2678 plant taxa listed in the national park. We identified 564 vascular plant species and subspecies (including nine hybrids) and one bryophyte to be included in the proposed protection list. The case study of the PNGSL could be a model for other protected areas.

Methodological Selection of Optimal Features for Object Classification Based on Stereovision System.

With the expansion of green energy, more and more data show that wind turbines can pose a significant threat to some endangered bird species. The birds of prey are more frequently exposed to collision risk with the wind turbine blades due to their unique flight path patterns. This paper shows how data from a stereovision system can be used for an efficient classification of detected objects. A method for distinguishing endangered birds from common birds and other flying objects has been developed and tested. The research focused on the selection of a suitable feature extraction methodology. Both motion and visual features are extracted from the Bioseco BPS system and retested using a correlation-based and a wrapper-type approach with genetic algorithms (GAs). With optimal features and fine-tuned classifiers, birds can be distinguished from aeroplanes with a 98.6% recall and 97% accuracy, whereas endangered birds are delimited from common ones with 93.5% recall and 77.2% accuracy.

Comparing the effectiveness of flagship species in zoo interpretation videos involving dialogic-based narrative approaches.

Zoological institutions frequently engage in indirect conservation activities as global conservation targets suggest a need for raising public awareness and engagement in biodiversity conservation. However, research suggests that while members of the public are typically aware of general conservation issues, they are often uncertain of simple and practical actions they take that will be impactful. In light of current conservation goals and targets, and the need for social science research to address the environmental learning and behavior change gap, this study builds upon prior action-based environmental education research and tests the efficacy of ex situ environmental education in supporting in situ conservation. Zoos typically employ flagship species to center their conservation messaging due to the purported draw of charismatic species. Using outreach videos with a dialogic-based narrative approach, we evaluated the efficacy of different flagships for conservation, comparing both species-focused versus generic conservation messaging and charismatic versus less charismatic species ambassadors. We found that zoo conservation outreach videos using dialogic-based narrative approaches were equally impactful regardless of the level of focus (i.e., species vs. broader biodiversity) or charisma level of the focal taxa.

Age and sex effects on histological features and in vitro culturing of Antillean manatee (Trichechus manatus manatus, Linnaeus 1758) dermis.

The biobanks from dermal biopsies represent an interesting strategy for biodiversity conservation. Nevertheless, the morphological and cellular patterns of the dermis can be influenced by the age and sex of the individual. Therefore, evaluating these factors is interesting for forming biobanks of Antillean manatees. These animals, representatives of marine fauna, have had their population reduced, and biobanks are essential for their conservation. Then, we evaluated the effects of age (3.5 years vs. 3.6-16 years vs. 23.6 years) and sex (males vs. females) on morphological and cellular parameters using histological and in vitro culture techniques. Regardless of age, no differences were observed for dermal thickness, collagen fibres, tissue proliferative activity and viable cell recovery. Nonetheless, fibroblast reduction was observed in groups aged 23.6 years compared to other animals (p < 0.05). Additionally, cells from animals aged 3.6-16 years showed more significant mitochondrial damage than the other groups (p < 0.05). Regardless of sex, no differences were observed for dermal thickness, collagen fibres, tissue proliferative activity and viable cell recovery; however, females had fewer fibroblasts than males (p < 0.05). Cells from females showed lower mitochondrial damage when compared to cells from males. In summary, although age and sex do not influence dermal thickness and cell recovery, variations in the number of fibroblasts and mitochondrial characteristics were observed among the groups. These differences may be significant for understanding the dermis aspects to be correlated to biobank systems.

 Myrsinecirrhosa (Primulaceae), a distinctive new shrub species from Kaua'i, Hawaiian Islands.

Myrsinecirrhosa Lorence & K.R.Wood (Primulaceae), a new single-island endemic shrub species from Kaua'i, Hawaiian Islands, is described and illustrated. Notes on its distribution, ecology and conservation status are included. The new species is known from an area with ca. 45 individuals, where it is restricted to the remote central windward region of Kaua'i in open bogs and along open windy ridges. Suggested IUCN Red List status is CR (Critically Endangered). It differs from its Kaua'i congeners by its longer petals and narrowly elliptic leaves with strongly undulate margins and tendril-like apex. Phylogenetic analysis using RADseq data supports the recognition of this new species.

Bio-Inspired Fluorescent Calcium Sulfate for the Conservation of Gypsum Plasterwork.

In this work, the potential of bio-inspired strategies for the synthesis of calcium sulfate (CaSO4·nH2O) materials for heritage conservation is explored. For this, a nonclassical multi-step crystallization mechanism to understand the effect of calcein- a fluorescent chelating agent with a high affinity for divalent cations- on the nucleation and growth of calcium sulfate phases is proposed. Moving from the nano- to the macro-scale, this strategy sets the basis for the design and production of fluorescent nano-bassanite (NB-C; CaSO4·0.5H2O), with application as a fully compatible consolidant for the conservation of historic plasterwork. Once applied to gypsum (CaSO4·2H2O) plaster specimens, cementation upon hydration of nano-bassanite results in a significant increase in mechanical strength, while intracrystalline occlusion of calcein in newly-formed gypsum cement improves its weathering resistance. Furthermore, under UV irradiation, the luminescence produced by calcein molecules occluded in gypsum crystals formed upon nano-bassanite hydration allows the easy identification of the newly deposited consolidant within the treated gypsum plaster without altering the substrate's appearance.

Livestock predation, crop raiding, and community attitudes towards sustainable wildlife conservation in and around Mankira Forest, Southwest Ethiopia.

Crop raiding and livestock predation negatively impact the views of the local community towards wildlife conservation. Farmers across the African continent, especially those in rural regions, incur financial losses as a result of crop raiding and livestock depredation. The sustainability of the forest relies heavily on comprehending the essential connection between a harmonious park-people relationship and the coexistence of humans and wildlife. The primary aim of this study is to evaluate the predation of livestock, the raiding of crops, and the attitudes of the community towards wildlife in the Mankira Forest located in southwest Ethiopia. This particular area has been lacking in scientific research, making it crucial to conduct this assessment. The data were collected between November 2021 and September 2022 via a structured questionnaire. This study used a sample of 241 randomly selected respondents from the four villages, and responses were compared using chi-square tests. Pearson correlation was also used to test the relationship between the distance of farmland and the extent of crop raiding. The majority of the respondents (95%) reported the presence of crop raiding and livestock predation in the area. These losses were caused by the Papio anubis (39%), the Chlorocebus aethiops (24.1%), the Hystrix cristata (15.3%), the Canis aures (58.3%), and the Crocutacrocuta (29.5%). Maize stood out as the crop type most susceptible to crop raiders. Most of the respondents (56.7%) had a negative attitude towards wildlife conservation. There was a significant difference among age groups of respondents related to their attitude towards wildlife conservation (p < 0.05). The study highlights the need to address several gaps in understanding and managing human-wildlife conflict through research on predation, raiding, and community attitudes. Therefore, to fulfill the dual goals of community support and conservation of wildlife, rigorous management and planning are needed.

Quality of Life after Mastectomy with or without Breast Reconstruction and Breast-Conserving Surgery in Breast Cancer Survivors: A Cross-Sectional Study at a Tertiary Hospital in Ghana.

(1) Background: Breast cancer is the leading malignancy worldwide, and in Ghana, it has a poor overall survival rate. However, approximately 50% of cases are cases of early-stage disease, and with advances in breast cancer treatment and improvements in survival, quality of life (QOL) is becoming as important as the treatment of the disease. (2) Methodology: This was a cross-sectional study of survivors who had breast-conserving surgery (BCS), mastectomy only (M) and mastectomy with breast reconstruction (BRS) from 2016 to 2020 at a tertiary hospital in Ghana, comparatively assessing their QOL using EORTC QLQ C-30 and EORTC QLQ BR-23. (3) Results: The study participants had an overall global health status (GHS) median score of 83.3 [IQR: 66.7-91.7] with no significant differences between the surgery types. The BRS group had statistically significant lower median scores for the functional scale (82.8 and 51.0) and the highest scores for the symptomatic scale (15.7 and 16.5). Body image was significantly lowest for the BRS group (83.3) [68.8-91.7] and highest (100) [91.7-100] for the BCS group (p < 0.001). (4) Conclusion: There is a need to develop support systems tailored at improving the QOL of breast cancer survivors taking into consideration the type of surgery performed.

3D documentation and classification of incense tree Aquilaria sinensis (Lour.) Spreng. wounds by photogrammetry and its potential conservation applications.

In recent years, illegal felling of and damage to the incense tree Aquilaria sinensis (Lour.) Spreng. have been reported in Hong Kong. Their native populations are under increasingly severe threat. Therefore, the development of a standard and efficient method to classify and document wounds on vulnerable trees is urgently needed for conservation purposes. In this study, photogrammetry was used to document wounds in A. sinensis through 3D modeling. A total of 752 wound records from 484 individual A. sinensis trees from Hong Kong were included to establish a new wound classification system. Our major findings include a novel standardized procedure for photogrammetric documentation and a wound classification system. The results of this study will facilitate A. sinensis conservation, by enhancing wound documentation and information transfer to law enforcement and education.

Forecasting climate change impacts on neotropical Myotis: Insights from ecological niche models for conservation strategies.

Myotis originated during the Oligocene in Eurasia and has become one of the most diverse bat genera, with over 140 species. In the case of neotropical Myotis, there is a high degree of phenotypic conservatism. This means that the taxonomic and geographic limits of several species are not well understood, which constrains detailed studies on their ecology and evolution and how to effectively protect these species. Similar to other organisms, bats may respond to climate change by moving to different areas, adapting to new conditions, or going extinct. Ecological niche models have become established as an efficient and widely used method for interpolating (and sometimes extrapolating) species' distributions and offer an effective tool for identifying species conservation requirements and forecasting how global environmental changes may affect species distribution. How species respond to climate change is a key point for understanding their vulnerability and designing effective conservation strategies in the future. Thus, here, we assessed the impacts of climate change on the past and future distributions of two phylogenetically related species, Myotis ruber and Myotis keaysi. The results showed that the species are influenced by changes in temperature, and for M. ruber, precipitation also becomes important. Furthermore, M. ruber appears to have been more flexible to decreases in temperature that occurred in the past, which allowed it to expand its areas of environmental suitability, unlike M. keaysi, which decreased and concentrated these areas. However, despite a drastic decrease in the spatial area of environmental suitability of these species in the future, there are areas of potential climate stability that have been maintained since the Pleistocene, indicating where conservation efforts need to be concentrated in the future.

Long-term data reveal fitness costs of anthropogenic prey depletion for a subordinate competitor, the African wild dog (Lycaon pictus).

Within carnivore guilds, dominant competitors (e.g., lions, Panthera leo) are limited primarily by the density of prey, while subordinate competitors (e.g., African wild dogs, Lycaon pictus) have been limited by the density of dominant competitors. Historically, the fitness and population density of subordinate competitors have not been tightly linked to prey density. However, populations of large herbivores have declined substantially across sub-Saharan Africa due to human impacts, and where prey depletion is severe, fitness costs for competitive subordinates may begin to outweigh the benefits of competitive release. Using long-term intensive monitoring of African wild dogs in Zambia's Luangwa Valley Ecosystem (LVE), we tested the effects of prey depletion on survival and reproduction. We hypothesized that African wild dog fitness would be lower in prey-depleted areas, despite lower lion densities. Our study area included four contiguous regions that varied in protection level, prey density, and lion density. We fit Bayesian Cormack-Jolly-Seber and closed-capture models to estimate effects on survival and population density, and generalized linear models to estimate effects on reproductive success. We found that the LVE is a stronghold for African wild dogs, with an estimated median density of 4.0 individuals/100 km2. Despite this high density, survival and reproduction differed among regions, and both components of fitness were substantially reduced in the region with the lowest prey density. Anthropogenic prey depletion is becoming an important limiting factor for African wild dogs. If prey depletion (or any other form of habitat degradation) becomes severe enough that its fitness costs outweigh the benefits of competitive release, such changes can fundamentally alter the balance between limiting factors for competitively subordinate species.

The devil is in the detail: Environmental variables frequently used for habitat suitability modeling lack information for forest-dwelling bats in Germany.

In response to the pressing challenges of the ongoing biodiversity crisis, the protection of endangered species and their habitats, as well as the monitoring of invasive species are crucial. Habitat suitability modeling (HSM) is often treated as the silver bullet to address these challenges, commonly relying on generic variables sourced from widely available datasets. However, for species with high habitat requirements, or for modeling the suitability of habitats within the geographic range of a species, variables at a coarse level of detail may fall short. Consequently, there is potential value in considering the incorporation of more targeted data, which may extend beyond readily available land cover and climate datasets. In this study, we investigate the impact of incorporating targeted land cover variables (specifically tree species composition) and vertical structure information (derived from LiDAR data) on HSM outcomes for three forest specialist bat species (Barbastella barbastellus, Myotis bechsteinii, and Plecotus auritus) in Rhineland-Palatinate, Germany, compared to commonly utilized environmental variables, such as generic land-cover classifications (e.g., Corine Land Cover) and climate variables (e.g., Bioclim). The integration of targeted variables enhanced the performance of habitat suitability models for all three bat species. Furthermore, our results showed a high difference in the distribution maps that resulted from using different levels of detail in environmental variables. This underscores the importance of making the effort to generate the appropriate variables, rather than simply relying on commonly used ones, and the necessity of exercising caution when using habitat models as a tool to inform conservation strategies and spatial planning efforts.

Genetic diversity and population structure of a rare flowering tree endemic to Appalachia, Stewartia ovata.

Stewartia ovata (cav.) Weatherby, commonly known as mountain stewartia, is an understory tree native to the southeastern United States (U.S.). This relatively rare species occurs in isolated populations in Virginia, Kentucky, Tennessee, North Carolina, South Carolina, Georgia, Alabama, and Mississippi. As a species, S. ovata has largely been overlooked, and limited information is available regarding its ecology, which presents obstacles to conservation efforts. Stewartia ovata has vibrant, large white flowers that bloom in summer with a variety of filament colors, suggesting potential horticultural traits prized by ornamental industry. However, S. ovata is relatively slow growing and, due to long seed dormancy, propagation is challenging with limited success rates. This has created a need to assess the present genetic diversity in S. ovata populations to inform potential conservation and restoration of the species. Here, we employ a genotyping-by-sequencing (GBS) approach to characterize the spatial distribution and genetic diversity of S. ovata in the southern Appalachia region of the eastern United States. A total of 4475 single nucleotide polymorphisms (SNPs) were identified across 147 individuals from 11 collection sites. Our results indicate low genetic diversity (He = 0.216), the presence of population structure (K = 2), limited differentiation (F ST = 0.039), and high gene flow (Nm = 6.16) between our subpopulations. Principal component analysis corroborated the findings of STRUCTURE, confirming the presence of two distinct S. ovata subpopulations. One subpopulation mainly contains genotypes from the Cumberland Plateau, Tennessee, while the other consists of genotypes present in the Great Smoky Mountain ranges in Tennessee, North Carolina, and portions of Nantahala, Chattahoochee-Oconee national forests in Georgia, highlighting that elevation likely plays a major role in its distribution. Our results further suggested low inbreeding coefficient (F IS = 0.070), which is expected with an outcrossing tree species. This research further provides necessary insight into extant subpopulations and has generated valuable resources needed for conservation efforts of S. ovata.

Endangered status and threatened population ecological factors in Salvia daiguii, an endemic species from Hunan, China.

Many species of Salvia have excellent ornamental, culinary, and medicinal values. Salvia daiguii, is an ornamental and highly medicinal perennial herb endemic to the prefecture-level city of Zhangjiajie in Hunan Province, China, with a narrow geographical distribution. However, currently, it has only been assessed as a Critically Endangered species according to the IUCN classification criteria, but its conservation has not yet been studied. This study investigated the distribution and niche characteristics of S. daiguii, and compared the differences in growth, flowering characteristics, and soil nutrients between the wild and ex situ populations. We also analyzed the effects of soil nutrients on plant growth and flowering characteristics. During the survey, we found 274 individuals on a rock approximately 200 m from ZEFR1. Nevertheless, S. daiguii were still restricted in three populations, TNFP, TGM, and ZEFR in Zhangjiajie City, with a total of about 500 plants and less than 250 mature individuals. Our results show that aspects such as adverse environmental conditions, low seedling renewal rate, a lack of soil nutrients, and competition for the characteristic niche of this and other dominant plants in the natural community are the main ecological factors affecting the growth, flowering, and geographic distribution of S. daiguii. Based on the results of field surveys, we recommend that (1) S. daiguii be classified as Critically Endangered C2b and China's List of Plant Species with Extremely Small Populations. (2) Comprehensive conservation strategies were developed, such as the establishment of nature reserves, reintroduction, public education, and institutional development to provide management recommendations related to the conservation of S. daiguii and other endangered plants.

Characterisation of the Cinnamomumparthenoxylon (Jack) Meisn (Lauraceae) transcriptome using Illumina paired-end sequencing and EST-SSR markers development for population genetics.

Cinnamomumparthenoxylon is an endemic and endangered species with significant economic and ecological value in Vietnam. A better understanding of the genetic architecture of the species will be useful when planning management and conservation. We aimed to characterize the transcriptome of C.parthenoxylon, develop novel molecular markers, and assess the genetic variability of the species. First, transcriptome sequencing of five trees (C.parthenoxylon) based on root, leaf, and stem tissues was performed for functional annotation analysis and development of novel molecular markers. The transcriptomes of C.parthenoxylon were analyzed via an Illumina HiSeqTM 4000 sequencing system. A total of 27,363,199 bases were generated for C.parthenoxylon. De novo assembly indicated that a total of 160,435 unigenes were generated (average length = 548.954 bp). The 51,691 unigenes were compared against different databases, i.e. COG, GO, KEGG, KOG, Pfam, Swiss-Prot, and NR for functional annotation. Furthermore, a total of 12,849 EST-SSRs were identified. Of the 134 primer pairs, 54 were randomly selected for testing, with 15 successfully amplified across nine populations of C.parthenoxylon. We uncovered medium levels of genetic diversity (PIC = 0.52, Na = 3.29, Ne = 2.18, P = 94.07%, Ho = 0.56 and He = 0.47) within the studied populations. The molecular variance was 10% among populations and low genetic differentiation (Fst = 0.06) indicated low gene flow (Nm = 2.16). A reduction in the population size of C.parthenoxylon was detected using BOTTLENECK (VP population). The structure analysis suggested two optimal genetic clusters related to gene flow among the populations. Analysis of molecular variance (AMOVA) revealed higher genetic variation within populations (90%) than among populations (10%). The UPGMA approach and DAPC divided the nine populations into three main clusters. Our findings revealed a significant fraction of the transcriptome sequences and these newlydeveloped novel EST-SSR markers are a very efficient tool for germplasm evaluation, genetic diversity and molecular marker-assisted selection in C.parthenoxylon. This study provides comprehensive genetic resources for the breeding and conservation of different varieties of C.parthenoxylon.

Analyzing the relative importance of habitat quantity and quality for boosting pollinator populations in agricultural landscapes.

To increase pollinator populations, international policy targets minimum levels of seminatural habitat cover, but it is unknown whether improving the quality of existing habitats could bring similar benefits without the need of reducing cropland area. Using data we collected in 26 Italian agricultural landscapes during the entire flying season, we explored the relative importance of habitat quantity (seminatural habitat cover) and quality (flower availability) on pollinator densities in seminatural habitats. We obtained transect-based counts and estimated the effect of habitat quantity (proportion of seminatural habitat) and quality (flower cover and richness) on wild bee and hoverfly densities. We used the relationships revealed in the data to simulate pollinator population sizes in landscapes with varying habitat quantity and quality. Wild bee densities were only related to flower availability, whereas hoverfly densities were additionally related to seminatural habitat cover. We found that in complex agricultural landscapes (above 15% seminatural habitat cover), improving habitat quality increased pollinator populations more effectively than increasing habitat quantity. However, increasing habitat quantity was by far the most effective approach for boosting pollinator populations in simple landscapes.

Análisis de la importancia relativa de la cantidad y calidad del hábitat para incrementar las poblaciones de polinizadores en los paisajes agrícolas Resumen Las políticas internacionales buscan que existan niveles mínimos de cobertura seminatural del hábitat para incrementar las poblaciones de polinizadores y se desconoce si mejorar la calidad de los hábitats existentes podría brindar beneficios similares sin tener que reducir el área de cultivo. Usamos datos recolectados en 26 paisajes agrícolas de Italia durante la temporada de vuelo para analizar la importancia relativa de la cantidad (cobertura de hábitat seminatural) y calidad (disponibilidad de flores) del hábitat para la densidad de polinizadores en los hábitats seminaturales. Obtuvimos conteos basados en transectos y estimamos el efecto de la cantidad (proporción del hábitat seminatural) y calidad (riqueza y cobertura de flores) del hábitat sobre la densidad de las abejas silvestres y los sírfidos. Usamos la relación revelada por los datos para simular el tamaño poblacional de los polinizadores en los paisajes con diferente calidad y cantidad de hábitat. La densidad de las abejas silvestres sólo se relacionó con la disponibilidad de flores cuando la densidad de sírfidos se relacionó con la cobertura del hábitat seminatural. Descubrimos que en los paisajes agrícolas complejos (por encima del 15% de cobertura de hábitat seminatural) cuya calidad mejoraba, las poblaciones de polinizadores incrementaban de manera más eficiente que cuando se mejoraba la cantidad. Sin embargo, incrementar la cantidad del hábitat fue por mucho la estrategia más efectiva para acrecentar las poblaciones de polinizadores en paisajes simples.

The dual protective role of accountability: Mitigating missed nursing care and nurse moral distress in a nested diary study design.

AIMS: To examine a novel moderated-mediation model, investigating whether personal accountability moderates the link between nurse workload and missed nursing care and whether missed nursing care mediates the association between workload and moral distress. DESIGN: Nested diary study. METHODS: Data spanning from February 2019 to February 2023 were collected from 137 nurses working in various inpatient wards in two medium-sized hospitals. Nurses reported care given to specific patients on three to five occasions across different shifts, establishing nurse-patient dyads. Validated measures of missed nursing care, personal accountability, moral distress and workload were analyzed using mixed linear models to test the nested moderated-mediation model. RESULTS: Under high workload conditions, nurses with higher personal accountability reported lower frequencies of missed nursing care compared to those with lower personal accountability. In contrast, under low workload conditions, personal accountability did not significantly influence missed nursing care occurrences. Furthermore, the interaction between workload and personal accountability indirectly affected nurses' moral distress through missed nursing care. Specifically, higher personal accountability combined with lower missed nursing care contributed to reduced levels of moral distress among nurses. CONCLUSION: The study highlights accountability's dual role-safeguarding against care omissions and influencing nurses' moral distress amid rising workload pressures. IMPLICATION FOR THE PROFESSION AND/OR PATIENT CARE: Cultivating a culture of accountability within healthcare settings can serve as a protective factor against the negative effects of workload on patient care quality and nurse psychological distress, highlighting the need for organizational interventions to promote accountability among nursing staff. IMPACT: By recognizing accountability's pivotal role, organizations can implement targeted interventions fostering accountability among nurses, including training programs focused on enhancing responsibility/ownership in care delivery and creating supportive environments prioritizing accountability to achieve positive patient outcomes. REPORTING METHOD: The study has adhered to STROBE guidelines. PATIENT OR PUBLIC CONTRIBUTION: No patient or public contribution.

Collaborative effect of the energy conservation and emission reduction fiscal policy in China.

Government-led national comprehensive demonstration cities for Energy Conservation and Emission Reduction Fiscal Policy (ECERFP) are pivotal for China in addressing environmental governance. Using a panel dataset covering 278 Chinese cities from 2003 to 2019, this study adopts the staggered difference-in-differences (DID) approach to investigate the synergistic impacts of ECERFP on pollution and carbon reduction. The findings indicate that ECERFP contributes to a 3% improvement in pollution reduction performance, a 1.5% enhancement in carbon reduction performance, and a 4% overall increase in combined pollution and carbon reduction efforts. Furthermore, the study examines the heterogeneous effects of ECERFP on environmental performance. ECERFP significantly influences the synergistic efforts in pollution and carbon reduction by fostering green innovation, enhancing energy allocation, and optimizing industrial structures. This study both theoretically and empirically outlines the specific pathways and mechanisms through which "incentive-based" green fiscal policy promotes synergistic pollution and carbon reduction, thus providing a pragmatic foundation for enhancing the role of fiscal policy in environmental governance.

Path for recovery: an ecological overview of the Jambato Harlequin Toad (Bufonidae: Atelopus ignescens) in its last known locality, Angamarca Valley, Ecuador.

The Jambato Harlequin toad (Atelopus ignescens), a formerly abundant species in the Andes of Ecuador, faced a dramatic population decline in the 1980s, with its last recorded sighting in 1988. The species was considered Extinct by the IUCN until 2016, when a fortuitous discovery of one Jambato by a local boy reignited hope. In this study, we present findings from an investigation conducted in the Angamarca parish, focusing on distribution, abundance, habitat preferences, ecology, disease susceptibility, and dietary habits of the species. In one year we identified 71 individuals at different stages of development in various habitats, with a significant presence in agricultural mosaic areas and locations near water sources used for crop irrigation, demonstrating the persistence of the species in a complex landscape, with considerable human intervention. The dietary analysis based on fecal samples indicated a diverse prey selection, primarily comprising arthropods such as Acari, Coleoptera, and ants. Amphibian declines have been associated with diseases and climate change; notably, our study confirmed the presence of the pathogen Batrachochytrium dendrobatidis (Bd), but, surprisingly, none of the infected Jambatos displayed visible signs of illness. When analyzing climatic patterns, we found that there are climatic differences between historical localities and Angamarca; the temporal analysis also exposes a generalized warming trend. Finally, in collaboration with the local community, we developed a series of management recommendations for terrestrial and aquatic environments occupied by the Jambato.

Metagenomics Analysis Reveals the Composition and Functional Differences of Fecal Microbiota in Wild, Farm, and Released Chinese Three-Keeled Pond Turtles (Mauremys reevesii).

The intestine of living organisms harbors different microbiota associated with the biological functioning and health of the host and influences the process of ecological adaptation. Here, we studied the intestinal microbiota's composition and functional differences using 16S rRNA and metagenomic analysis in the wild, farm, and released Chinese three-keeled pond turtle (Mauremys reevesii). At the phylum level, Bacteroidota dominated, followed by Firmicutes, Fusobacteriota, and Actinobacteriota in the wild group, but Chloroflexi was more abundant in the farm and released groups. Moreover, Chryseobacterium, Acinetobacter, Comamonas, Sphingobacterium, and Rhodobacter were abundant in the released and farm cohorts, respectively. Cetobacterium, Paraclostridium, Lysobacter, and Leucobacter showed an abundance in the wild group. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that the relative abundance of most pathways was significantly higher in the wild turtles (carbohydrate metabolism, lipid metabolism, metabolism of cofactors, and vitamins). The comprehensive antibiotic resistance database (CARD) showed that the antibiotic resistance gene (ARG) subtype macB was the most abundant in the farm turtle group, while tetA was higher in the wild turtles, and srpYmcr was higher in the released group. Our findings shed light on the association between the intestinal microbiota of M. reevesii and its habitats and could be useful for tracking habitats to protect and conserve this endangered species.