Insights into the Biological Activities and Substituent Effects of Pyrrole Derivatives: The Chemistry-Biology Connection.

Pyrrole, with its versatile heterocyclic ring structure, serves as a valuable template for generating a diverse range of lead compounds with various pharmacophores. Researchers and scientists globally are intrigued by pyrrole and its analogues for their broad pharmacological potential, prompting thorough investigations aimed at advancing human welfare. This comprehensive review delves into the diverse activities exhibited by pyrrole compounds, encompassing their synthesis, reactions, and pharmacological properties alongside their derivatives. In addition to detailing the characteristics of pyrrole and its derivatives within the context of green chemistry, the review also examines microwave-assisted reactions. It provides insights into their chemical structures, natural occurrences, and potential applications across various domains. Furthermore, the article investigates structural alterations of pyrrole compounds and their implications on their functionality, highlighting their versatility as foundational elements for both functional materials and bioactive compounds. Ultimately, the review underscores the imperative for continued research and development in the realm of pyrrole compounds to unearth novel activities and advantages.

Antioxidant polysaccharide-enriched fractions obtained from olive leaves by ultrasound-assisted extraction with α-amylase inhibition, and antiproliferative activities.

Polysaccharide-rich materials were extracted from the alcohol-insoluble solids of Olea europaea l. **leaves. Structural characteristics were determined by colorimetric techniques, FT-IR, GC-MS, SEC/MALS/VD/DRI, and NMR (1H,13C). The extract and its main macromolecular components were characterized to assess their ability toward antioxidant, α-amylase inhibition, and antiproliferative activities. Results revealed that the ultrasound olive leave extract comprises polysaccharides with uronic acid, galactose, arabinose, and glucose in molar percentages of 11.7%, 11.3%, 7.5%, and 4.9% respectively, constituting 41% of the total mass. In addition, polyphenols (21%) and proteins (9%) are associated with these polysaccharides. Further, the extract showed noticeable ORAC and free radical scavenging abilities, in addition to high in vitro antiproliferative activity against Caco-2 colon carcinoma cell lines. Similarly, the extract exhibited a strong, uncompetitive inhibition of α-amylase by 75% in the presence of the extract with 0.75 µg/mL of concentration. This research concludes that ultrasound extraction method can be used for the extraction of polysaccharide-polyphenol-protein complexes. These conjugates exhibit the potential for combined biological activities resulting from a synergistic effect of its compounds, making them promising ingredients for the development of functional food.

Application of metagenomic next-generation sequencing (mNGS) in diagnosing pneumonia of adults.

INTRODUCTION: Accurate identification of pathogens that cause pulmonary infections is essential for effective treatment and hastening recovery in adults diagnosed with pneumonia. At present, despite metagenomic next-generation sequencing (mNGS) technology has been widely used in clinical practice for pathogen identification, the clinical significance and necessity of detecting pathogen in bronchoalveolar lavage fluid (BALF) for pneumonia-stricken adults remain ambiguous. METHODOLOGY: In this study, 80 patients suffering from pulmonary infection were enrolled, who were admitted to the Affiliated Changzhou Second People's Hospital of Nanjing Medical University between January 2020 and September 2022. The diagnostic performances of mNGS and conventional methods (CM) were systematically analyzed based on BALF samples, and we further investigated the influence of mNGS and CM in diagnosis modification and treatment. RESULTS: We found a significantly higher positive rate for the mNGS method in contrast to CM. Bacteria were the most common pathogens, and Streptococcus pneumoniae was the most commonly identified pathogen. Candida albicans and Epstein-Barr virus were the most frequently identified fungus and virus. Atypical pathogens such as Mycobacterium tuberculosis, virus Nontuberculous mycobacteria, and Chlamydia psittaci were also identified. A total of 77 patients were identified with mixed infections by mNGS. As the disease progressed and recurrent antibiotic treatment persisted, significant dynamic changes in the clinical manifestation from the BALF samples could be found by mNGS. CONCLUSIONS: This study underscores the efficacy of mNGS in detecting pathogens in BALF samples from patients suffering pulmonary infections. Compared with the CM, mNGS significantly enhanced the positive diagnosis ratio, particularly in diagnosing Mycobacterium tuberculosis, atypical pathogens, and viral or fungal infections.

The Impact of Team Triage Method on Emergency Department Performance Indexes: A quasi-interventional study.

Introduction: One of the effective methods of patient triage in the emergency department (ED) is the use of team triage, including physicians and nurses. Considering that there is no conclusive evidence about the effectiveness of team triage, this study aimed to investigate the effect of the team triage method on ED performance indexes. Methods: The present study is a quasi-interventional study in which 200 patients were referred to the ED in the hospitals of Tabriz in 2020. Participants were randomly assigned to two groups (team triage and conventional triage) and were evaluated. Data were collected by a three-part questionnaire including the participants' demographic characteristics, the five-level triage form, and Press-Ganey satisfaction questionnaire were used. Data were analyzed by SPSS.22 statistical software. Results: The results showed that the mean score of waiting time for the first physician visit in team triage was statistically significantly lower than the conventional triage (P = 0.001). Furthermore, the mean score of waiting time for receiving the first treatment in team triage was statistically significantly lower than the conventional triage (P = 0.001). Finally, the mean score of patients' satisfaction in team and conventional triage was statistically significantly higher in team triage (P = 0.001). Conclusion: The study findings revealed that the team triage method, in comparison to conventional triage, decrease the waiting time for receiving the first service and length of stay, but leads to more patient's satisfaction. Therefore, to improve the performance indicators of the ED, it is recommended that hospital managers use the team triage method.

Objective Structured Practical Examination (OSPE) as a Tool for the Formative Assessment of Practical Skills in the Subject of Physiology: A Comparative Cross-Sectional Analysis.

BACKGROUND: Practical assessments hold a critical role in evaluating medical education. However, achieving objectivity, consistency, authenticity, reliability, and practical usefulness in student evaluations can be a formidable challenge. The Objective Structured Practical Examination (OSPE) stands out as a promising technique tailored to assess performance in a realistic educational setting. OSPE offers a unique approach to aligning assessment methods with the educational objectives of a given activity, making it possible to comprehensively gauge the attainment of pedagogical goals. OBJECTIVE: This study aimed to overcome the limitations associated with traditional practical tests and explore the potential advantages of OSPE in improving the objectivity, consistency, authenticity, and reliability of student evaluations in the context of medical education. Through a comparative analysis, this research endeavors to illuminate the practical applicability of OSPE. The primary goal of this research was to introduce and assess the feasibility of employing the OSPE as a formative assessment tool for appraising the practical capabilities of Physiology students. METHODOLOGY:  Fifty students from 1st year MBBS were included in this study after their written consent. They were divided into two groups of 25 students each; two practical procedures, (a) hemoglobin estimation, and (b) performing blood group. Students were assessed at two different sessions. Students of each group assessed by the conventional method in the first session were assessed by OSPE in the second session of the same practical and vice versa. At the completion of the assessment process, both students and teachers were asked to rate the various assessment techniques on a Likert scale. Student test results and instructor and student opinions were statistically examined using the paired t-test. A significance level of 0.05 was used. RESULTS:  When evaluated using the OSPE method, students obtained significantly higher mean marks (12.58±2.74) compared to the conventional assessment method (8.44±2.13). A paired t-test confirmed the statistical significance of the improvement in student performance with OSPE (p<0.0001). Student feedback indicated strong agreement (92%) that OSPE encourages greater focus on practical examinations and is an effective assessment and learning method. Teachers expressed unanimous agreement that OSPE is a more comprehensive evaluation tool (100%) and better at highlighting student strengths and weaknesses (75%). The majority of teachers (75%) believed that OSPE should be incorporated into future examinations. CONCLUSION:  The study demonstrates that OSPE significantly enhances student performance and is well-received by both students and teachers as a more effective and comprehensive assessment method.

Comparison of the conventional and immersing powdered crude drugs (IPCD) methods for color and extraction of quantitative indicator ingredients in the Kampo formula decoction of daiokanzoto.

AIM: The immersing powdered crude drugs (IPCD) method is a quick and simple method for preparing decoctions. Here, the conventional and IPCD methods were compared for the color and extraction of quantitative indicator ingredients in the daiokanzoto decoction solution, and the suitability of the IPCD method was assessed. METHODS: The color of decoction solutions was visually observed, and the Commission Internationale de L'éclairage (CIE) L*a*b*color parameters were measured using conventional and IPCD methods. The extracted amounts of sennoside A and glycyrrhizic acid, which are quantitative indicator ingredients of rhubarb and glycyrrhiza, respectively, were quantified. RESULTS: Using both methods, the decoction solution colors were strong for rhubarb alone and daiokanzoto but weak for glycyrrhiza alone. The color change of daiokanzoto was thought to be primarily caused by rhubarb alone. The L*a*b* values of the decoction solution determined by the IPCD method were comparable to those determined by the conventional method (60 min). Using the conventional method, sennoside A and glycyrrhizic acid were mostly extracted in 10 and 30 min, respectively. Using the IPCD method, both sennoside A and glycyrrhizic acid were fully extracted in 2 min. The IPCD method yielded significantly more sennoside A and glycyrrhizic acid (2 times and 1.5 times, respectively) than the conventional method (60 min). CONCLUSION: The IPCD method was found to be comparable to the conventional method in terms of the color, and using IPCD method, the same or greater amounts of quantitative indicator ingredients of crude drugs in the decoction of daiokanzoto compared to the conventional method. It was suggested that there are limitations to assessing the equivalence of decoctions from decoction color. The IPCD method may be a useful method although it is prudent to use the IPCD method for Kampo formula decoction in clinical practice with a certain degree of caution.

Mechanism Models of the Conventional and Advanced Methods of Construction Safety Training. Is the Traditional Method of Safety Training Sufficient?

Cognitive failures at the information acquiring (safety training), comprehension, or application stages led to near-miss or accidents on-site. The previous studies rarely considered the cognitive processes of two different kinds of construction safety training. Cognitive processes are a series of chemical and electrical brain impulses that allow you to perceive your surroundings and acquire knowledge. Additionally, their attention was more inclined toward the worker's behavior during hazard identification on-site while on duty. A study is proposed to fill the knowledge gap by developing the mechanism models of the two safety training approaches. The mechanism models were developed based on cognitive psychology and Bloom's taxonomy and six steps of cognitive learning theory. A worker's safety training is vital in acquiring, storing, retrieving, and utilizing the appropriate information for hazard identification on-site. It is assumed that those trained by advanced techniques may quickly identify and avoid hazards on construction sites because of the fundamental nature of the training, and when they come across threats, they may promptly use their working memory and prevent them, especially for more complex projects. The main benefit of making such a model, from a cognitive point of view, is that it can help us learn more about the mental processes of two different types of construction safety training, and it can also help us come up with specific management suggestions to make up for the approaches' flaws. Future research will concentrate on the organizational aspects and other cognitive failures that could lead to accidents.

A comparative clinical trial for evaluating the posterior palatal seal developed from the conventional method and a novel functional swallow method.

Aims: The aim of this in vivo study was to compare the influence of posterior palatal seal (PPS) developed from the conventional method and a novel functional swallow method on the retention of custom tray and heat cure denture base. Settings and Design: This was a nonrandomized crossover clinical trial. Materials and Methods: Twenty patients requiring maxillary complete dentures were selected. In Group 1, for all twenty patients, the PPS was developed with the conventional functional method during border molding and a conventional cast scoring was performed before processing the denture base. In Group 2, for all the twenty patients, the PPS was developed with a novel functional swallow method and the master cast was "not" scored before processing the denture base. The retention was objectively measured using a dynamometer after border molding and also after processing the denture base for both groups. Statistical Analysis Used: Independent Student's t-test and paired t-test were used for analysis. Results: The mean retention value of Group 2 was significantly higher (P < 0.001) than Group 1 at border molding and after denture base processing. Within Group 1, the retention value significantly increased (P < 0.001) from border molding to the denture base stage, whereas within Group 2, there was no significant change (P > 0.001) between the stages. Conclusions: Within the limitations of the study, the novel functional swallow method of establishing the PPS demonstrated higher retention than the conventional method both during border molding and after processing the denture base.

Patient-specific instrumentation for total knee arthroplasty improves reproducibility in the planned rotational positioning of the tibial component.

BACKGROUND: The purpose of this study was to evaluate the reproducibility of planned component positioning including tibial rotational alignment in patient-specific instrumentation (PSI) for total knee arthroplasty (TKA). METHODS: A total of 100 knees of 100 patients underwent TKA using PSI (n = 50) or the conventional method (n = 50). Full-length anteroposterior radiographs of the lower limb were taken in the standing position, and the coronal alignments of the femoral and tibial components were measured. Computed tomography (CT) images of the lower limb were obtained preoperatively and postoperatively, and the rotational alignments of the femoral and tibial components were measured. The difference from the preoperative planning in tibial rotational alignment was measured using three-dimensionally merged pre- and postoperative images. The mean values and rates of outliers in each measurement were compared between the PSI group and the conventional group. RESULTS: There were no significant differences in coronal alignment of the femoral and tibial components and rotational alignment of the femoral component between the two groups. With respect to rotational alignment of the tibial component from the preoperatively planned reference axis, the PSI group showed a lower rate of outliers (internal rotation > 10°) than the conventional group (p < 0.05). CONCLUSIONS: This study demonstrated that the difference from the preoperative planning in tibial rotational positioning was accurately evaluated using novel three-dimensional measurement method, and PSI could reduce outliers in rotational alignment of the tibial component (internal rotation > 10°). PSI is a useful technique for improving the reproducibility of the planned tibial rotational positioning in TKA.

Fabrication Methods of Electroactive Scaffold-Based Conducting Polymers for Tissue Engineering Application: A Review.

Conductive scaffolds, defined as scaffold systems capable of carrying electric current, have been extensively researched for tissue engineering applications. Conducting polymers (CPs) as components of conductive scaffolds was introduced to improve morphology or cell attachment, conductivity, tissue growth, and healing rate, all of which are beneficial for cardiac, muscle, nerve, and bone tissue management. Conductive scaffolds have become an alternative for tissue replacement, and repair, as well as to compensate for the global organ shortage for transplantation. Previous researchers have presented a wide range of fabrication methods for conductive scaffolds. This review highlights the most recent advances in developing conductive scaffolds, with the aim to trigger more theoretical and experimental work to address the challenges and prospects of these new fabrication techniques in medical sciences.

Comprehensive technological assessment for different treatment methods of leather tannery wastewater.

The leather-making process necessitates large amounts of water and consequently generates tons of liquid waste as leather tannery wastewater (TWW) is disposed of directly in the open environment. Open disposal of untreated TWW into the natural environment causes an accumulation of various polluting compounds, including heavy metals, dyes, suspended solids inorganic matter, biocides, oils, tannins, and other toxic chemicals. It thus poses potential hazards to the environment and human health. This study primarily focuses on providing in-depth insight into the characteristics, treatment strategies, and regulatory frameworks for managing TWW in leather processing industries. Different technologies of conventional physico-chemical (equalization, coagulation, and adsorption), advanced approaches (Fenton oxidation, ozonation, cavitation), thermo-catalytic and biological treatments available to treat TWW, and their integrative approaches were also highlighted. This review also sheds light on the most frequently applied technologies to reduce contaminant load from TWW though there are several limitations associated with it such as being ineffective for large quantities of TWW, waste generation during treatment, and high operational and maintenance (O&M) costs. It is concluded that the sustainable alternatives applied in the current TWW technologies can minimize O&M costs and recirculate the treated water in the environment. The exhaustive observations and recommendations presented in this article are helpful in the industry to manage TWW and recirculate the water in a sustainable manner.

Present and pioneer methods of early detection of food borne pathogens.

Food-borne pathogens are a severe threat to human illness and death world-wide. Researchers have reported more than 250 food-borne diseases. Most of these are infections caused by a wide variety of bacteria, viruses, and parasites. It has a significant economic impact also. Detection of pathogenic microbes is thus essential for food safety. Such identification techniques could meet the following parameters viz., the accuracy of detection techniques that are quick, efficient, economical, highly sensitive, specific, and non-labor intensive. The various available methods for detecting food pathogens are classified into different groups, each having its advantages and disadvantages. The conventional methods are usually the first choice of detection even though they are laborious. Modern techniques such as biosensors, immunological assays, and macromolecule-based (nucleic acid) methods are being developed and refined to overcome traditional methods' limitations. Early detection of pathogens and secure food safety at each stage of food processing to storage, utilizing improved methodologies are mandatory. This review summarizes the deadly food pathogens leading to significant outbreaks and discusses the importance of early detection methods and advanced detection methods in comparison.

Harmful Microalgae Detection: Biosensors versus Some Conventional Methods.

In the last decade, there has been a steady stream of information on the methods and techniques available for detecting harmful algae species. The conventional approaches to identify harmful algal bloom (HAB), such as microscopy and molecular biological methods are mainly laboratory-based and require long assay times, skilled manpower, and pre-enrichment of samples involving various pre-experimental preparations. As an alternative, biosensors with a simple and rapid detection strategy could be an improvement over conventional methods for the detection of toxic algae species. Moreover, recent biosensors that involve the use of nanomaterials to detect HAB are showing further enhanced detection limits with a broader linear range. The improvement is attributed to nanomaterials' high surface area to volume ratio, excellent biological compatibility with biomolecules, and being capable of amplifying the electrochemical signal. Hence, this review presents the potential usage of biosensors over conventional methods to detect HABs. The methods reported for the detection of harmful algae species, ranging from conventional detection methods to current biosensor approaches will be discussed, along with their respective advantages and drawbacks to indicate the future prospects of biosensor technology for HAB event management.

A Comparative Study Across Methods to Identify Adolescents with Syntactic Specific Language Impairment.

Background: Specific language impairment (SLI) is described as a heterogeneous deficit that causes difficulties in various aspects of language. We performed a comparative study of two methods of language assessment with the primary objective of determining the most effective approach for identifying adolescents with syntactic SLI and typical development (TD) in use. Methods: A software-assisted method using E-Prime 2.0 was used to create an experiment. The participants were Malay adolescents aged 13 years old-15 years old. The conventional method was compared with the software-assisted method to assess the participants' comprehension and production performance. Data on reaction time (RT), scoring and no response (NR) were obtained from the adolescents. Results: Based on the two methods, the findings on the selection of participants for the SLI and TD groups was different. The two methods produced similar results in terms of the selection of TD group and most participants in the syntactic SLI group except for two participants who failed in the conventional method but passed the test in the software-assisted method. Conclusion: The descriptive evaluation of the findings suggested selecting software-assisted method as the alternative source because the provided information was detailed and this information enabled the researcher to identify the SLI group.

Antifungal profile of vulvovaginal candidiasis in sexually active females from a tertiary care hospital of Western Rajasthan.

INTRODUCTION: Vulvovaginal candidiasis (VVC) is the commonest form of sexually transmitted infection especially in sexually active females. Various species of Candida i.e., Candida albicans and non-albicans Candida are associated with VVC. More than 75% of women experiences vulvovaginal candidiasis at least once in their lifetime and 10% of it can lead to recurrent VVC. So, this study was planned to evaluate the clinico-mycological profile and antifungal profile of VVC in sexually active female attending tertiary care hospital. MATERIALS AND METHODS: The present two months study was conducted in sexually active females attending Obstetrics -gynecology OPD with VVC in tertiary care hospital. Two high vaginal swabs were taken and fungal culture was done on SDA agar by standard methods. Identification and antifungal susceptibility testing of candidial isolates were done by standard mycological methods. RESULTS: Most of the patients belonged to younger age group between 18 and 29 years (55%). Lower abdominal pain was the most common symptom after vaginal discharge followed by burning sensation and pruritis. Candida glabrata (15) with 58% of all the isolates was the most common Candida species associated with VVC in this study, followed by Candida albicans (5, 19%). Highest antifungal resistance was observed to itraconazole (81%) followed by amphotericin B (35%) and fluconazole (31%). 81% resistance to itraconazole among Candida glabrata and Candida albicans. Voriconazole was maximum susceptible to all Candida species. CONCLUSION: This study highlights the incidence of VVC among sexually active females of reproductive age group as its recurrence may result into obstetric complications and even infertility and also enlightens the common Candida species and their antifungal profile, which would help the treating clinicians to formulate local antifungal treatment policy for VVC.

Comparing novel versus conventional technique of platelet-rich plasma therapy in periorbital hyperpigmentation: A randomized prospective split-face study.

BACKGROUND: Platelet-rich plasma (PRP) has been found to be effective in treating periorbital hyperpigmentation (POH). PRP prepared by double-spin (DS) method and activated by calcium has been used conventionally. PRP can be prepared by single spin (SS) and activated at low temperature (novel method), but the evidence is limited. OBJECTIVE: To compare the novel and conventional PRP in the treatment of periorbital hyperpigmentation. METHODS: We selected 21 patients of POH and randomly divided the face into two halves. One-half of the face (group A) was treated with novel PRP (SS and low-temperature activation). The other half (group B) was treated with conventional PRP (DS and calcium activation). A total of 3 PRP injections were given at 4 weekly intervals. Patients were observed and assessed on 12th week by photography, dermoscopy, visual analog scale (VAS) score, and Dermatology life quality index (DLQI). Platelet counts and growth factors were assessed in PRP. RESULTS: Mean platelet count in novel and conventional PRP was 7.41 ± 1.76 lacs and 8.17 ± 2.23 lacs (p = 0.348). Mean photographic and dermoscopic assessment at the end of the study in group A and group B was 52.33 ± 6.468 and 53.14 ± 6.99 (p = 0.151). Change in VAS in groups A and B was 3.85 ± 1.27 and 3.90 ± 1.04 (p = 0.895). Levels of various growth factors assessed by ELISA did not differ significantly. There was significant decline in DLQI. CONCLUSION: The novel method is not inferior to conventional method of PRP in the treatment of periorbital hyperpigmentation.

Marginal and Internal Fit of Ceramic Restorations Fabricated Using Digital Scanning and Conventional Impressions: A Clinical Study.

This clinical study was designed with the aim of fabricating four ceramic crowns using the conventional method and digital methods with three different intraoral scanners and evaluate the marginal and internal fit as well as clinician satisfaction. We enrolled 20 subjects who required ceramic crowns in the upper or lower molar or the premolar. Impressions were obtained using digital scans, with conventional impressions (polyvinyl siloxane and desktop scanner) and three different intraoral scanners (EZIS PO, i500, and CS3600). Four lithium disilicate glass-ceramic crowns were fabricated for each patient. In the oral cavity, the proximal and occlusal adjustments were performed, and the marginal fit and internal fit were evaluated using the silicone replica technique. The clinician satisfaction score of the four crowns was evaluated as per the evaluations of the proximal and occlusal contacts made during the adjustment process and the marginal and internal fit. For statistical analysis, the differences among the groups were analyzed with one-way analysis of variance and Tukey HSD test as a post-test; Pearson correlation analysis was used for analyzing the correlations (α = 0.05). There was a significant difference in the marginal and internal fit of the ceramic crowns fabricated using three intraoral scanner types and one desktop scanner type (p < 0.001); there was a significant difference in the clinician satisfaction scores (p = 0.04). The clinician satisfaction score and marginal fit were significantly correlated (absolute marginal discrepancy and marginal gap) (p < 0.05). An impression technique should be considered for fabricating a ceramic crown with excellent goodness-of-fit. Further, higher clinician satisfaction could be obtained by reproducing the excellent goodness-of-fit using the intraoral scanning method as compared to the conventional method.

Are postoperative pain and patient satisfaction influenced by the number of ports in VATS for malignant pleural effusion treatment?

Objectives: The aim of this research is by using sociological methods of scientific research for tracking the pain and satisfaction indicators to prove that decreasing the number of ports in the VATS (Video-assisted thoracic surgery) for Malignant Pleural Effusion reduces postoperative pain and improves patient's satisfaction.Methods: Our study included 117 VATS procedures performed in the period from 01 January 2013 to 31 September 2016. The sociological method used to measure the pain indicator was an interview. The severity of postoperative pain was determined and reported according to a ten-point pain visual analogue scale (VAS). The degree of satisfaction was determined and reported according to a six-point and ten-point grading systems on the basis of a research interview procedure.Results: In the single-port method, the verbal pain scale for all the days covered by the research study statistically showed significantly lower values (P Ë‚ 0.0001) in comparison with the conventional method (P Ë‚ 0.0001). With regard to the patient's satisfaction, determined on the basis of the six-point system, the results were as follows: conventional VATS approach - average 3.1 with a standard deviation of 1.1 and ranging from 0 to 5; single-port VATS approach - average 4.3 with a standard deviation of 1.0 and within the range from 0 to 6. Conventional VATS approach - 6.8 - neutral. Single-port VATS approach - 8.1 - prevailing satisfaction.Conclusions: Based on our study and the studies of other authors, it can be concluded that postoperative pain and satisfaction after VATS in patients with MPE (Malignant pleural effusion) are influenced by the number of ports and the one-port technique shows better results than the conventional three-port method.The research study was registered and approved by the Clinical Research and Ethics Committee at the 'Prof. Dr. Stoyan Kirkovich' AD University Multi-Profile Hospital for Active Treatment Hospital, Stara Zagora. According to Protocol No. 11, Ref. No. 12471/30.10.2015 approved are the methods used by the sociological research study which uses predefined indicators to track patients who have undergone conventional VATS and single-port VATS. Indicators: postoperative pain and satisfaction.

DNA-strand molecular beacon optical processor.

Due to the characteristics of the newly developed DNA computing, many researchers are interested in this specialty. One advantage of DNA " Deoxyribonucleic acid" is that it has ability to resolve a Boolean circuit with various types of gates at the same time in a single level. Most of the prior models suffered from the limitations that each level of the circuit requests the gates to be of some kind. The model proposed in this work increases parallelism and reduces human intervention to a tremendous extent. When level-wise simulation is executed, the simulation for each model shows the decrease in the number of nitrogen bases used, which leads to the processing of the largest number of data with the ability to increase the length of a word, in addition to the adoption of the parallel principle of implementation. The model is designed on a mechanism which includes adder and multiplier.

The difference of detection rate of avian influenza virus in the wild bird surveillance using various methods.

Korea is located within the East Asian-Australian flyway of wild migratory birds during the fall and winter seasons. Consequently, the likelihood of introduction of numerous subtypes and pathotypes of the Avian influenza (AI) virus to Korea has been thought to be very high. In the current study, we surveyed wild bird feces for the presence of AI virus that had been introduced to Korea between September 2017 and February 2018. To identify and characterize the AI virus, we employed commonly used methods, namely, virus isolation (VI) via egg inoculation, real-time reverse transcription-polymerase chain reaction (rRT-PCR), conventional RT-PCR (cRT-PCR) and a newly developed next generation sequencing (NGS) approach. In this study, 124 out of 11,145 fresh samples of wild migratory birds tested were rRT-PCR positive; only 52.0% of VI positive samples were determined as positive by rRT-PCR from fecal supernatant. Fifty AI virus specimens were isolated from fresh fecal samples and typed. The cRT-PCR subtyping results mostly coincided with the NGS results, although NGS detected the presence of 11 HA genes and four NA genes that were not detected by cRT-PCR. NGS analysis confirmed that 12% of the identified viruses were mixed-subtypes which were not detected by cRT-PCR. Prevention of the occurrence of AI virus requires a workflow for rapid and accurate virus detection and verification. However, conventional methods of detection have some limitations. Therefore, different methods should be combined for optimal surveillance, and further studies are needed in aspect of the introduction and application of new methods such as NGS.