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Introduction: Interspecies interactions are a crucial driving force of species evolution. The genes of each coexisting species play a pivotal role in shaping the structure and function within the community, but how to identify them at the genome-wide level has always been challenging. Methods: In this study, we embed the Lotka-Volterra ordinary differential equations in the theory of community ecology into the systems mapping model, so that this model can not only describe how the quantitative trait loci (QTL) of a species directly affects its own phenotype, but also describe the QTL of the species how to indirectly affect the phenotype of its interacting species, and how QTL from different species affects community behavior through epistatic interactions. Results: By designing and implementing a co-culture experiment for 100 pairs of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), we mapped 244 significant QTL combinations in the interaction process of the two bacteria using this model, including 69 QTLs from E. coli and 59 QTLs from S. aureus, respectively. Through gene annotation, we obtained 57 genes in E. coli, among which the genes with higher frequency were ypdC, nrfC, yphH, acrE, dcuS, rpnE, and ptsA, while we obtained 43 genes in S. aureus, among which the genes with higher frequency were ebh, SAOUHSC_00172, capF, gdpP, orfX, bsaA, and phnE1. Discussion: By dividing the overall growth into independent growth and interactive growth, we could estimate how QTLs modulate interspecific competition and cooperation. Based on the quantitative genetic model, we can obtain the direct genetic effect, indirect genetic effect, and genome-genome epistatic effect related to interspecific interaction genes, and then further mine the hub genes in the QTL networks, which will be particularly useful for inferring and predicting the genetic mechanisms of community dynamics and evolution. Systems mapping can provide a tool for studying the mechanism of competition and cooperation among bacteria in co-culture, and this framework can lay the foundation for a more comprehensive and systematic study of species interactions.
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Based on knowledge sharing, a new kind of scientific research is up and coming in university interdisciplinary research teams via the current environment of organizational models. The success, however, depends on the knowledge inventory, the creative ability of the team members and their future insights. An attempt is made in this study to conceptualize a framework of an interdisciplinary research team based on game theory to analyze the dynamic propagation process of knowledge-sharing. Through simulation verification, a multi-symmetry evolution game model was built to analyze the impact of a member in selecting a decision-making strategy for the other member. The analysis reveals that the knowledge-sharing depends on mutual cooperation and trust between the researchers. Finally, reasonable suggestions are proposed in solving the problems in the process of building and developing the university interdisciplinary research team.
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Aerobic methane oxidation coupled with denitrification (AME-D) executed in membrane biofilm bioreactors (MBfRs) provides a high promise for simultaneously mitigating methane (CH4) emissions and removing nitrate in wastewater. However, systematically experimental investigation on how oxygen partial pressure affects the development and characteristics of counter-diffusional biofilm, as well as its spatial stratification profiles, and the cooperative interaction of the biofilm microbes, is still absent. In this study, we combined Optical Coherence Tomography (OCT) with Confocal Laser Scanning Microscopy (CLSM) to in-situ characterize the development of counter-diffusion biofilm in the MBfR for the first time. It was revealed that oxygen partial pressure onto the MBfR was capable of manipulating biofilm thickness and spatial stratification, and then managing the distribution of functional microbes. With the optimized oxygen partial pressure of 5.5 psig (25% oxygen content), the manipulated counter-diffusional biofilm in the AME-D process obtained the highest denitrification efficiency, due mainly to that this biofilm had the proper dynamic balance between the aerobic-layer and anoxic-layer where suitable O2 gradient and sufficient aerobic methanotrophs were achieved in aerobic-layer to favor methane oxidation, and complete O2 depletion and accessible organic sources were kept to avoid constraining denitrification activity in anoxic-layer. By using metagenome analysis and Fluorescence in situ hybridization (FISH) staining, the spatial distribution of the functional microbes within counter-diffused biofilm was successfully evidenced, and Rhodocyclaceae, one typical aerobic denitrifier, was found to survive and gradually enriched in the aerobic layer and played a key role in denitrification aerobically. This in-situ biofilm visualization and characterization evidenced directly for the first time the cooperative path of denitrification for AME-D in the counter-diffused biofilm, which involved aerobic methanotrophs, heterotrophic aerobic denitrifiers, and heterotrophic anoxic denitrifiers.
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Denitrification , Methane , Biofilms , Bioreactors , In Situ Hybridization, Fluorescence , Oxidation-Reduction , OxygenABSTRACT
Currently, urbanization driven by global capital flows entails a main trend in many large cites in China, while global capital investment in small inland cities especially in western China is extremely scarce, where their globalization characters the powerful nationalization power and market activation. Dunhuang, a small inland city in western China, has transformed successfully from an agricultural county to an international tourist city, a platform for worldwide cultural communication, and a node city in the Belt and Road region because of its unique and brilliant resources: Mogao Grottoes and Dunhuangology. Therefore, this paper develops a conceptual framework of the multiple cooperative mechanisms and globalization path (MCMGP) of Dunhuang, elaborating the process of industrial transformation, urban globalization, and multiple cooperative mechanisms between government and market actors based on interviewing records and statistics. Findings show that the MCMGP features government-led intervention, resource orientation, and centralization that embodies the driver of state-owned enterprises (SOEs). Also, the MCM in Dunhuang's globalization contains the mechanism of enrolment, mobilization and action, governance and global marketing, distributed in the two phases. Equally important, in response to the Belt and Road Initiative (BRI) and Silk Road (Dunhuang) International Cultural Expo (SRDICE) from the state, the city government has significantly reinvested and refined cultural tourism via governance mechanisms, carving out a key node city in the Silk Road and elevating an international tourist city. Environmentally, Dunhuang's tourism internationalization enhances the process of the development of a sustainable shared mobility industry. Furthermore, its tourism development and social-ecology system maintain the synergistic relationships which international tourism promotes such as urban ecosystem and public welfare and in turn, social-ecological enhancement serve Dunhuang's international tourism well. Practical implications of how Dunhuang's experience may have lessons for others are discussed in China's peculiar socialist market economy discourse.
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Ecosystem , Internationality , China , Cities , Economic Development , SilkABSTRACT
The pollution control of the Rhine is a successful management mode for comprehensive cooperation in environmental governance, which could provide an important reference for the Yangtze River governance. This study explored river basin management and various pollution problems faced by these two rivers at different times, and also includes governance and management effects. The changes in water quality and aquatic life before and after policy implementation had also been compared. The results showed that the International Commission for the Protection of the Rhine (ICPR) made outstanding contributions to the water-quality improvement, demonstrating its management mode was efficient and effective. Cooperative communication and targeted policies are effective ways to improve the water quality of large river basins. Clear division of labor system and phased cooperation-governance objectives are also significant for the inter-provincial cooperative governance of Yangtze River pollution.
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Conservation of Natural Resources , Rivers , China , Environmental Monitoring , Environmental Policy , EuropeABSTRACT
From the perspective of industrial clusters, the rapid development of the Chinese medicinal materials industry faces the challenges of improving quality and efficiency and transforming and upgrading. The core problem is how to improve the anti-risk ability, resilience, and competition of the Chinese medicinal material supply chain through collaborative management of the supply chain. The particularity of Chinese medicinal materials determines the formation of Chinese medicinal materials industrial clusters with specific natural, social, and industrial conditions. From the perspective of industrial clusters, the supply chain of Chinese medicinal materials also presents unique structural characteristics. Based on the analysis of the formation conditions of the traditional Chinese medicine industry cluster and the structural characteristics of the traditional Chinese medicine supply chain, on the basis of clarifying the connotation of collaborative management of the traditional Chinese medicine supply chain from the perspective of the industrial cluster, it is proposed to establish a supply chain collaborative management mechanism at the macro level, the meso level reconstructs the supply chain business process system and optimizes the supply chain function coordination mode at the micro level to build a collaborative management system for the supply chain of Chinese medicinal materials.
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The construction of medical big data includes several problems that need to be solved, such as integration and data sharing of many heterogeneous information systems, efficient processing and analysis of large-scale medical data with complex structure or low degree of structure, and narrow application range of medical data. Therefore, medical big data construction is not only a simple collection and application of medical data but also a complex systematic project. This paper introduces China's experience in the construction of a regional medical big data ecosystem, including the overall goal of the project; establishment of policies to encourage data sharing; handling the relationship between personal privacy, information security, and information availability; establishing a cooperation mechanism between agencies; designing a polycentric medical data acquisition system; and establishing a large data centre. From the experience gained from one of China's earliest established medical big data projects, we outline the challenges encountered during its development and recommend approaches to overcome these challenges to design medical big data projects in China more rationally. Clear and complete top-level design of a project requires to be planned in advance and considered carefully. It is essential to provide a culture of information sharing and to facilitate the opening of data, and changes in ideas and policies need the guidance of the government. The contradiction between data sharing and data security must be handled carefully, that is not to say data openness could be abandoned. The construction of medical big data involves many institutions, and high-level management and cooperation can significantly improve efficiency and promote innovation. Compared with infrastructure construction, it is more challenging and time-consuming to develop appropriate data standards, data integration tools and data mining tools.
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Big Data , China , Data MiningABSTRACT
The anaerobic ammonia oxidation (anammox) and sulfocompound-oxidizing autotrophic denitrification coupling system (A/SAD) was initiated in an expanded granular sludge bed (EGSB) reactor for nitrogen removal from high-strength wastewater. Owing to cooperation between anammox and partial sulfocompound-oxidation autotrophic denitrification coupling system (PSAD), the highest nitrogen removal efficiency (NRE) of 98.1%⯱â¯0.4% achieved at the optimal influent conditions of conversion efficiency of ammonium (CEA) of 55% and S2O32--S/NO3--N (S/N) of 1.4â¯molâ¯mol-1. The activity of the short-cut sulfocompound-oxidizing autotrophic denitrification (SSAD) was also regulated to cope with dynamic CEA in the influent by changing the S/N, which was demonstrated to be effective in alleviating nitrite accumulation when the CEA was between 57% and 61%. Both the anammox and SAD bacteria enriched in the reactor after long-term incubation. Candidatus Brocadia and Candidatus Jettenia might be potentially contributing the most to anammox, while the Thiobacillus was the dominant taxa related to SAD.
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Bioreactors , Denitrification , Anaerobiosis , Autotrophic Processes , Nitrogen , Oxidation-Reduction , SewageABSTRACT
Traditional Chinese medicine (TCM) has been the focus and highlight of the ancient Silk Road trade activities since ancient times. Since the Belt and Road Initiative was proposed for the first time in China in 2013, the internationalized development of TCM has made positive progress. Based on the theory of international mobility of production factors, this paper qualitatively analyzes the "five mechanisms" of international cooperation between China and the countries along the routes in the background of the Belt and Road Initiative: The intergovernmental exchange and cooperation mechanism; cooperation mechanism of TCM inheritance and innovation; TCM service trade cooperation mechanism; TCM resources sharing cooperation mechanism; cooperation mechanism of TCM culture and humanities exchanges. A qualitative and quantitative analysis combined with partial equilibrium analysis were used to analyze the welfare effects before and after the production factors of TCM transnational movement, and make a further investigation on the difficulties and challenges faced by the current international cooperation of TCM in China, and put forward the corresponding countermeasures and suggestions.
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Objective To explore the management of the development of medical research.Methods This paper analyzes the construction mode and experience of the National Center for Clinical Medicine Research in promoting the transformation of medical research,and discusses the cooperation mechanism,talent construction,performance evaluation and institution construction.Results Based on proposed development stage medical institutions and resources,and gradually improve the "transformation chain" management thinking.Conclusions Medical institutions should rely on basic and clin ical resources,based on the concept of medical transformation,strengthen the top design,and gradually change the management model.
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Objective:To study the problem of the interests decision-making about sharing medical resources between private hospitals and public hospitals research,and to explore the internal symbiosis mechanism of private hospitals and public hospitals.Methods:The maximization method was applied to study in depth the decision-making and benefit distribution mechanism of private bospitals and public hospitals.Results:The symbiotic cooperation of private hospitals and public hospitals could bring the total additional benefits of the medical service market when the supply of the sharing medical resources was greater than demand.Collaborative decision was better than independent decision-making between private hospitals and public hospitals.When the supply of sharing in medical resources was less than demand situation,the symbiotic cooperation decisions of both hospitals could not bring additional benefits from the medical service market.Conclusion:In order to make full use of medical resources,provide better service to the residents and maximize the total benefit of the medical service market,it was recommended to build the information platform,form effctive coordination mechanism and establish collaborative performance evaluation.
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Objective:To analyze the concerning guarantee mechanisms during the transformation of the com-munity health service mode. Method:Purposive sampling was adopted. Twelve community health service centers in Beijing, Shanghai, Zhengzhou, and Chengdu, where the transformation of the community health service mode was pi-loted earlier and representative, were selected as the field survey sites. The qualitative method was used to collect da-ta accompanied by the quantitative method. Results: The guarantee mechanisms related to the transformation of the community health service mode could be concluded into four main types:collaboration mechanism, health profession-al training mechanism, incentive mechanism, and policy guarantee mechanism. All of the four mechanisms contribu-ted to the improvement of general teamwork, dual referral systems, and the development of contract and appointment services. Conclusion:During the transformation of the community health service mode, priority strategies include top-down design, health professional training mechanisms, and performance-based incentive mechanism, all of which should be implemented in the future.
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Energy theory of drug and cooperation mechanism of energy for active ingredients of Chinese materia medica (CMM) were put forward Concepts of "correlation energy of disease" and "synthetic Chinese formulated medicine" were given The third way: "simplicity→complexity" for modernization of CMM was proposed Science theory in Chinese formulated medicine was discussed by cooperation mechanism of energy for active ingredients which suggested that CMM be indispensable for recovery of some diseases With the aid of computing chemistry, molecular simulation, biology informatics and pharmacokinetics, it is possible to investigate and confirm correlation energy of different diseases and combinations of active ingredients of Chinese formulated medicines Problems of different producing areas, confusions of species and instability of CMM quality can be solved by returning from chemical synthetic drug to synthesize the Chinese formulated medicine
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OBJECTIVE:To discuss the relation between sino-foreign cooperation mechanism and the internationalization of Chinese medicine products. METHODS: We discussed the positive correlation between sino-foreign cooperation mechanism and the internationalization of Chinese medicine products in theory and practice. RESULTS & CONCLUSION: The internationalization of Chinese medicine products can be achieved by setting up sino-foreign cooperation mechanism in Chinese medicine industry according to WTO regulations.
