Enhancing polycyclic aromatic hydrocarbon soil remediation in cold climates using immobilized low-temperature-resistant mixed microorganisms.

Polycyclic aromatic hydrocarbons (PAHs), widespread organic pollutants, significantly impact human health and environmental integrity. Recent approaches to ameliorate PAH-contaminated soils, particularly in cold environments, have been insufficient. This study investigates the use of immobilized low-temperature-resistant mixed microorganisms (LTRMM) for enhancing the degradation of PAHs in soils from coke plants and the Shenfu irrigation area. Our results demonstrate that treatment with immobilized mixed microorganisms (MC-HS) is more effective than treatments with free bacteria (H-S) and control (CK). Specifically, the degradation rates in the MC-HS1 treatment were 10.10 %-41.13 % higher than those in the coking plant soil treated with CK1 and H-S1. Similarly, in the Shenfu irrigation area soil, MC-HS2 showed improvements of 6.00 % to 52.56 % over CK2 and H-S2. A kinetic model was used to analyze the enhanced degradation capabilities, revealing that the half-life of PAHs under the immobilized mixed microorganism treatment (T3) was significantly shorter compared to the free bacteria (T2) and control treatments (T1). These findings suggest that employing immobilized LTRMM could significantly improve the remediation efficiency of PAH-contaminated soils in cold climates.

An anthocyanin-rich extract from Zea mays L. var. ceratina alleviates neuronal cell death caused by hydrogen peroxide-induced cytotoxicity in SH-SY5Y cells.

The incidence of dementia is rising, with neuronal cell death from oxidative stress and apoptosis recognized as a significant contributor to its development. However, effective strategies to combat this condition are lacking, necessitating further investigation. This study aimed to assess the potential of an anthocyanin-rich extract from Zea mays L. var. ceratina (AZC) in alleviating neuronal cell death.Neurotoxicity was induced in SH-SY5Y cells using hydrogen peroxide (H2O2) at a concentration of 200 µM. Cells were pretreated with varying doses (31.25 and 62.5 µg/mL) of AZC. Cell viability was assessed using the MTT assay, and molecular mechanisms including reactive oxygen species (ROS) levels, antioxidant enzyme activities (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px)), malondialdehyde (MDA) levels for oxidative stress, and the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2), cAMP response element-binding protein (CREB), and apoptotic factors (B-cell lymphoma 2 (Bcl-2), caspase 3) were explored.Results showed that AZC significantly improved cell viability, reduced ROS production and MDA levels, and downregulated caspase 3 expression. It enhanced CAT, SOD, and GSH-Px activities, activated ERK1/2 and CREB, and upregulated Bcl-2 expression. These findings support the neuroprotective effects of AZC, suggesting it activates ERK1/2, leading to CREB activation and subsequent upregulation of Bcl-2 expression while suppressing caspase 3. AZC may mitigate neuronal cell death by reducing ROS levels through enhanced scavenging enzyme activities.In conclusion, this study underscores the potential of AZC as a neuroprotective agent against neuronal cell death. However, further investigations including toxicity assessments, in vivo studies, and clinical trials are necessary to validate its benefits in neuroprotection.

Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli.

Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a platform substance with diversified applications. To eliminate catabolite repression and enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding glucose transporter EIICBGlc and pgi encoding phosphoglucose isomerase were deleted. With four heterologous enzymes including xylose dehydrogenase, xylonolactonase, xylonate dehydratase, α-ketoacid decarboxylase and endogenous YqhD, E. coli BT-10 can produce 36.63 g/L 1,2,4-butanetriol with a productivity of 1.14 g/[L·h] using xylose as substrate. When corn cob hydrolysate was used as the substrate, 43.4 g/L 1,2,4-butanetriol was generated with a productivity of 1.09 g/[L·h] and a yield of 0.9 mol/mol. With its desirable characteristics, E. coli BT-10 is a promising strain for commercial 1,2,4-butanetriol production.

Preparation and extraction property study of corn cob-like magnetic mosaic carbon materials derived from MOF-on-MOF composites.

BACKGROUND: Large accumulations of pesticide residues in the environment eventually enter the human body with food. Based on magnetic solid-phase extraction (MSPE) technology, it is possible to achieve efficient extraction of trace pesticide residues in foodstuffs, and the construction of MSPE adsorbents with excellent magnetic properties and many active sites is still one of the main research topics. Based on this, we developed a new strategy for the preparation of "MOF-on-MOF" composites, which were carbonized and used as adsorbents for the extraction of pesticide residues in cereals. RESULTS: A novel corn cob core-shell composite Fe2O3@C@ZIF-8 was created by embedding ZIF-8 onto the surface of Fe2O3@C derived from MIL-88A(Fe), and used for the extraction of benzoylurea insecticides(BUs) from cereals. The adsorption behaviour between Fe2O3@C@ZIF-8 and BUs was investigated by static and kinetic experiments and the adsorption mechanism was elaborated. For the trace analysis of BUs, a magnetic solid-phase extraction combined with high performance liquid chromatography-ultraviolet detector (HPLC-UV) approach was also developed. Under the optimized experimental conditions, the limits of detection and quantification were 0.015-0.03 µg L-1 and 0.05-0.1 µg L-1, and the relative standard deviations for the intra-day and inter-day ranges were 1.82%-2.13 % and 3.85%-4.59 %, respectively. The spiked recoveries of the four cereals ranged from 82.72% to 104.45 %. After 10 cycles of use of Fe2O3@C@ZIF-8, the recoveries of BUs ranged from 77.90% to 96.74 %. SIGNIFICANCE: The analytical method based on Fe2O3@C@ZIF-8 as adsorbent has a low limit of detection, a wide linear range and is highly applicable to the analysis of real samples. In addition, the strategy developed in this study for the preparation of thermoplastic polymer-assisted "MOF-on-MOF" composites has promising applications.

Performance and mechanism of benzene adsorption on ZnCl2 one-step modified corn cob biochar.

Utilizing cost-effective corn cob, zinc chloride-modified biochar was synthesized through one-step method for benzene adsorption from air. Study on impregnation ratio impact showed optimal benzene adsorption at ZnCl2:CC ratio of 1.5:1, with capacity reaching 170.53 mg g-1. Characterization using BET, SEM, FTIR, and XPS was conducted. BET results indicated specific surface area of Zn1.5BC at 1260.63 m2 g-1 and maximum pore volume of 0.546 m3 g-1. SEM analysis revealed microporous-mesoporous structure in Zn1.5BC, marking significant improvement over original biomass. DFT pore size distribution and FTIR analysis suggested post-modification dehydration and elimination reactions, leading to volatile compound release, functional group reduction, and pore widening. XPS analysis showed decrease in O = C-OH content with increased impregnation ratio, enhancing biochar's π-π electron diffusion for benzene. Langmuir isotherm and pseudo-second-order kinetic models effectively described experimental data, indicating multilayer benzene adsorption on biochar controlled by complex physicochemical adsorption and pore diffusion. Adsorption condition assessment, including adsorption temperature (20-120 â„ƒ) and benzene concentration in inlet phase (159.73-383.36 mg L-1), was performed. Yoon-Nelson model fitting indicated adsorption site loss at higher temperatures and reduced capture ability due to increased adsorbate molecule kinetic energy. Higher adsorbate concentrations aided adsorption molecule diffusion to biochar surface and internal pores, increasing adsorption rate and shortening equilibrium time. Overall, zinc chloride-modified biochar facilitates benzene adsorption through pore filling and π-π interactions, with pore filling as primary mechanism. Produced biochar shows excellent regeneration properties and reusability.

Preparation and Improvement of Physicochemical and Functional Properties of Dietary Fiber from Corn Cob Fermented by Aspergillus niger.

Corn cobs were fermented with Aspergillus niger to produce soluble dietary fiber (SDF) of high quality and excellent food safety. In this work, the fermentation process was optimized by single-factor test and response surface methodology (RSM). The optimal fermentation conditions were determined to be a material-liquid ratio of 1:30, an inoculum concentration of 11%, a temperature of 32°C, a time of 6 days, and a shaking speed of 200 r/min. Under these conditions, the SDF yield of corn cob increased from 2.34% to 11.92%, and the ratio of soluble dietary fiber to total dietary fiber (SDF/TDF) reached 19.08%, meeting the requirements for high-quality dietary fiber (SDF/TDF of more than 10%). Scanning electron microscopy (SEM) and Fourier-transformed infrared spectroscopy (FT-IR) analysis revealed that the fermentation effectively degraded part of cellulose and hemicellulose, resulting in the formation of a loose and porous structure. After fermentation the water swelling capacity, water-holding capacity, and oil-holding capacity of the corn cob SDF were significantly improved and the adsorption capacity of glucose, cholesterol, and nitrite ions all increased by more than 20%. Moreover, the total phenolic content increased by 20.96%, which correlated with the higher antioxidant activity of SDF. Overall, the fermentation of corn cobs by A. niger increased the yield and enhanced the functional properties of dietary fiber (DF) as well.

Adsorption of aflatoxin B1 to corn by-products.

The adsorption of aflatoxin B1 (AFB1) to natural fiber materials prepared from corn by-products was investigated in this study. The results showed that corn cob powder (CCP) dose, particle size, time (0.25-24 h), temperature (4, 20, 37, 50 and 100 °C) and pH (2-8), had significant effects on adsorption. The maximum adsorption (98%) was with particles 500-355 µm in size at 20 °C for 8 h, at the dose of 50 mg mL-1. The adsorption fitted pseudo-second-order model and Langmuir isotherm well. Besides, CCP had a higher adsorption capacity to AFB1 than any single cell wall components of corn, which indicated that capillary effect happened in cell wall might be the main reason for adsorption. The results also suggested that CCP could reduce AFB1 content from both liquid and solid food matrixes. Briefly, CCP displayed promising properties that could be developed in nature-based practical applications for food aflatoxin decontamination.

Immobilized Cellulase on NH2-MIL-88(Fe) and Its Performance as a Biocatalyst.

To broaden pH range and improve thermal stability, reusability, storage stability, and organic solvent tolerance of natural enzymes, a magnetic material (NH2-MIL-88(Fe)) was synthesized as a new material to immobilize cellulase. The results showed that the optimal temperature and pH of cellulase immobilized on NH2-MIL-88(Fe) showed a wider range compared to free cellulase, and 74% and 83% of the initial activity could be retained after 10 cycles and storage for 49 days, respectively. Moreover, the tolerance for organic solvents was improved compared with free enzyme. The reducing sugar yields from sodium carboxymethylcellulose (CMC) and corn cob hydrolyzed with cellulase immobilized on NH2-MIL-88(Fe) were higher than observed with the free enzyme, which demonstrated the better biocatalytic performance of cellulase immobilized on NH2-MIL-88(Fe).

Environmentally Friendly Masonry Mortar Blended with Fly Ash, Corn Cob Ash or Ceramic Waste Powder.

Implementing a circular approach through waste valorization in mortar production with environmentally efficient mix design is a viable pathway for relieving the ecological burden of greenhouse gas emissions, resource depletion and waste management. The main objective of this paper is to evaluate the feasibility of using fly ash (FA), corn cob ash (CCA), and ceramic waste powder (CWP) as supplementary cementitious materials (SCM) in cement-lime masonry mortars. As part of an extensive experimental study, twelve mortar mixtures were made: three reference and nine blended, with mixing ratios of 1:1:5, 1:0.7:4.2, and 1:1:4 ((cement + SCM)/lime/sand), by volume. The examined properties include workability, compressive and flexural strengths, dry bulk density, capillary water absorption, adhesive bond strength, and water vapor permeability. The compressive and flexural strengths of tested mortars were notably impaired, with reductions of up to 60%, while the capillary water absorption coefficient rose by 100% compared to the reference values. The adhesive bond strength of some blended mortars exceeded the strength of the reference mortars. Nevertheless, all blended mortars fulfilled the requirements for general-purpose mortars, while the majority met the criteria for structural masonry applications. In addition, a performance-based index and weighting triangle were used for the comparison and ranking of all analyzed mortar mixtures. The findings of this study may herald a novel use of FA, CCA, and CWP as more eco-friendly binding materials in contemporary construction leading to the reduction in the process's carbon footprint, the improvement in cost efficiency, and the mitigation of the detrimental environmental impact of waste disposal.

Corn cob hemicelluloses as stabilizer for ice recrystallization inhibition in ice cream.

Food stabilizers, such as guar gum and locust bean gum (LBG), are often added to ice cream to improve its texture and to combat its main shelf-life concern - ice recrystallization. Recently these gums have become increasingly expensive due to the limited supplies. In this study, holocellulose nanocrystals (holoCNCs) and hemicelluloses (hemiCs) were prepared from readily available corn cobs and tested for ice recrystallization inhibition (IRI) activities in the 25.0 % sucrose solution and ice cream mixes (ICMs). In the sucrose solution, holoCNCs were not IRI active at a concentration of 0.5 %, but hemiCs demonstrated a good IRI activity, even at 0.1 %. In the ICMs, the IRI activity of hemiCs was better than those of guar gum and LBG at a concentration of 0.2 %. Adding 0.2-0.5 % hemiCs had no negative influences on the physicochemical properties of ICMs and ice cream, including viscosity profile, particle size distribution, overrun, hardness, and meltdown rate. These research findings demonstrated corn cob hemiCs' potential as a more sustainable ice cream stabilizer.

Electrochemical Performance of Corn Waste Derived Carbon Electrodes Based on the Intrinsic Biomass Properties.

The exploration of cost-effective and sustainable biomass-derived carbon materials as electrodes for energy conversion and storage has gained extensive attention in recent research studies. However, the selection of the biomass and the electrochemical performance regulation of the derived biochar, as well as their interrelationship still remain challenging for practical application. Herein, corn wastes with high carbon content (>40%), corn cob and corn silk, were selected as precursors for the preparation of high value-added and high yield carbon materials via a modified synthetic process. Uniquely, this work put emphasis on the theoretical and experimental investigations of how the biomass properties influence the composition and nanostructure regulation, the electrolyte ion adsorption free energy, and the electrical conductivity of the derived carbon materials as well as their electrochemical performance optimization. Owing to the favorable specific surface area, the hierarchical porous structure, and the diverse elemental distribution, corn cob and corn silk derived carbon materials (CBC and SBC) present great potential as promising electrodes for alkaline aqueous zinc batteries and supercapacitors. The assembled CBC//Zn and SBC//Zn zinc batteries deliver high energy densities of 63.0 Wh kg-1 and 39.1 Wh kg-1 at a power density of 575 W kg-1, with excellent cycling performance of 91.1% and 84.3% capacitance retention after 10,000 cycles. As for the assembled symmetric supercapacitors, high energy densities of 14.9 Wh kg-1 and 13.6 Wh kg-1, and superior long-term cycling stability of 99.3% and 96.6% capacitance retention after 20,000 cycles could be achieved. This study highlights the advantages of utilizing corn cob and corn silk as carbon sources on the designed synthesis of carbon electrodes, and presents a meaningful perspective in the investigation of biomass-derived carbon materials and their potential applications in rechargeable devices.

Development of intelligent and active potato starch films based on purple corn cob extract and molle essential oil.

The present study aims to develop an active and "intelligent" film that uses potato starch as a polymeric matrix, anthocyanins from purple corn cob as a natural dye, and molle essential oil as an antibacterial compound. The color of anthocyanin solutions is pH-dependent, and the developed films show a visual color change from red to brown after immersion in solutions with pH values ranging from 2 to 12. SEM and FTIR analyses suggested that anthocyanins have favorable dispersibility and good compatibility with the starch-glycerol matrix. The study found that both anthocyanins and molle essential oil significantly enhanced the ultraviolet-visible light barrier performance. Tensile strength, elongation at break, and elastic modulus reached values of 3.21 MPa, 62.16 %, and 12.87 MPa, respectively. The biodegradation rate in vegetal compost also accelerated during the three-week period, achieving a weight loss of 95 %. Moreover, the film presented an inhibition halo for Escherichia coli, indicating its antibacterial activity. The results suggest that the developed film has the potential to be used as food-packaging material.

High-level production of Aspergillus niger prolyl endopeptidase from agricultural residue and its application in beer brewing.

BACKGROUND: Prolyl endopeptidase from Aspergillus niger (AN-PEP) is a prominent serine proteinase with various potential applications in the food and pharmaceutical industries. However, the availability of efficient and low-cost AN-PEP remains a challenge owing to its low yield and high fermentation cost. RESULTS: Here, AN-PEP was recombinantly expressed in Trichoderma reesei (rAN-PEP) under the control of the cbh1 promoter and its secretion signal. After 4 days of shaking flask cultivation with the model cellulose Avicel PH101 as the sole carbon source, the extracellular prolyl endopeptidase activity reached up to 16.148 U/mL, which is the highest titer reported to date and the secretion of the enzyme is faster in T. reesei than in other eukaryotic expression systems including A. niger and Komagataella phaffii. Most importantly, when cultivated on the low-cost agricultural residue corn cob, the recombinant strain was found to secret a remarkable amount of rAN-PEP (37.125 U/mL) that is twice the activity under the pure cellulose condition. Furthermore, treatment with rAN-PEP during beer brewing lowered the content of gluten below the ELISA kit detection limit (< 10 mg/kg) and thereby, reduced turbidity, which would be beneficial for improving the non-biological stability of beer. CONCLUSION: Our research provides a promising approach for industrial production of AN-PEP and other enzymes (proteins) from renewable lignocellulosic biomass, which provides a new idea with relevant researchers for the utilization of agricultural residues.

Magnetic Biochar Obtained by Chemical Coprecipitation and Pyrolysis of Corn Cob Residues: Characterization and Methylene Blue Adsorption.

Biochar is a carbonaceous and porous material with limited adsorption capacity, which increases by modifying its surface. Many of the biochars modified with magnetic nanoparticles reported previously were obtained in two steps: first, the biomass was pyrolyzed, and then the modification was performed. In this research, a biochar with Fe3O4 particles was obtained during the pyrolysis process. Corn cob residues were used to obtain the biochar (i.e., BCM) and the magnetic one (i.e., BCMFe). The BCMFe biochar was synthesized by a chemical coprecipitation technique prior to the pyrolysis process. The biochars obtained were characterized to determine their physicochemical, surface, and structural properties. The characterization revealed a porous surface with a 1013.52 m2/g area for BCM and 903.67 m2/g for BCMFe. The pores were uniformly distributed, as observed in SEM images. BCMFe showed Fe3O4 particles on the surface with a spherical shape and a uniform distribution. According to FTIR analysis, the functional groups formed on the surface were aliphatic and carbonyl functional groups. Ash content in the biochar was 4.0% in BCM and 8.0% in BCMFe; the difference corresponded to the presence of inorganic elements. The TGA showed that BCM lost 93.8 wt% while BCMFe was more thermally stable due to the inorganic species on the biochar surface, with a weight loss of 78.6%. Both biochars were tested as adsorbent materials for methylene blue. BCM and BCMFe obtained a maximum adsorption capacity (qm) of 23.17 mg/g and 39.66 mg/g, respectively. The obtained biochars are promising materials for the efficient removal of organic pollutants.

High yield production of nanocrystalline cellulose from corn cob through a chemical-mechanical treatment under mild conditions.

Agricultural biomass waste such as corn cob is available in large quantities and can be used as renewable materials for various applications. Corn cob was converted into nanocrystalline cellulose by using mild sulfuric acid concentrations (30 % w/v) at low temperature (50 °C) and a relatively shorter time extraction (30 min) combined with mechanical treatment using a conventional high-speed blender. NCC from cellulose and α-cellulose from corn cobs have been successfully isolated with relatively high yields and crystallinities of 50.07-65.33 % and 65.5-69.9 %, respectively. Scanning electron microscopy (SEM) evaluated the morphological variation and dimension from corn cob fiber (CF), delignification fiber (DF), cellulose, and α-cellulose, which shows that each pretreatment stage causes a decrease in fiber diameter from 16.56 to 5.48 µm. Transmission electron microscopy (TEM) images confirmed the nano-scale dimension with fiber diameters ranging between 9.35 nm and 6.51 nm. Thermogravimetric analysis shows that NCC has relatively high thermal stability ranging from 429 to 437 °C. Thus, this characteristic of NCC has the potential to be applied as a reinforcing agent in various fields of polymer composites. Finally, this study presents a method for isolating NCC from corncob waste using a conventional high-speed blender in a mild condition process with a relatively low cost, environmentally friendly pathway, and high yield that was still preserved.

Structural Characterization, In Vitro Digestion Property, and Biological Activity of Sweet Corn Cob Polysaccharide Iron (III) Complexes.

This study aimed to enhance the utilization value of sweet corn cob, an agricultural cereal byproduct. Sweet corn cob polysaccharide-ron (III) complexes were prepared at four different temperatures (40 °C, 50 °C, 60 °C, and 70 °C). It was demonstrated that the complexes prepared at different temperatures were successfully bound to iron (III), and there was no significant difference in chemical composition; and SCCP-Fe-C demonstrated the highest iron content. The structural characterization suggested that sweet corn cob polysaccharide (SCCP) formed stable ß-FeOOH iron nuclei with -OH and -OOH. All the four complexes' thermal stability was enhanced, especially in SCCP-Fe-C. In vitro iron (III) release experiments revealed that all four complexes were rapidly released and acted as iron (III) supplements. Moreover, in vitro antioxidant, α-glucosidase, and α-amylase inhibition studies revealed that the biological activities of all four complexes were enhanced compared with those of SCCP. SCCP-Fe-B and SCCP-Fe-C exhibited the highest in vitro antioxidant, α-glucosidase, and α-amylase inhibition abilities. This study will suggest using sweet corn cobs, a natural agricultural cereal byproduct, in functional foods. Furthermore, we proposed that the complexes prepared from agricultural byproducts can be used as a potential iron supplement.

Effects of Grinding Methods and Water-to-Binder Ratio on the Properties of Cement Mortars Blended with Biomass Ash and Ceramic Powder.

To combat environmental challenges-such as the depletion of natural resources and a high carbon footprint-and contribute to the effort of achieving zero-waste technology and sustainable development, the use of agricultural and industrial wastes in the cement industry has created a research interest. This study explores the potential of two types of harvest residue ash (HRA) and three types of ceramic waste (CP) as supplementary cementitious materials (SCMs) through: (1) the characterization of raw materials and (2) examining the physical properties and mechanical performance of cement-based mortar samples prepared with 10%, 30% and 50%wt of the selected SCMs ground into powder form as cement replacement. Two main variables were the water-to-binder ratio (w/b) and the effect of different grinding procedures. Experimental results demonstrated that flexural and compressive strengths were not significantly impaired by SCM additions of up to 50%, but higher replacement levels led to an increased permeability and higher capillary water absorption due to the dilution effect. Also, a lower w/b was shown to effectively reduce the porosity of mortar and increase its mechanical properties, allowing for higher shares of SCMs to be utilized. This study verifies the technical feasibility of cob corn ash and ceramic powder application as SCMs in mortar formulations, further promoting the practice of incorporating industrial and agricultural by-products in greener cementitious composites.

Denitrification efficiency, microbial communities and metabolic mechanisms of corn cob hydrolysate as denitrifying carbon source.

In this study, the denitrification efficacy of corn cob hydrolysate (CCH) was compared and analyzed with that of glucose and acetate to determine its feasibility as an additional carbon source, and its metabolic mechanism as a denitrification carbon source was investigated in depth. By constructing a denitrification reactor, it was found that the TN removal rate exceeded 97% and the effluent COD remained below 70 mg/L during the stable operation with CCH as the carbon source, and the denitrification effect was comparable to that of the glucose stage (GS) and the acetate stage (AS). The analysis of the microbial community showed that the dominant phylum was Proteobacteria and Bacteroidota, where the abundance of Bacteroidota in the hydrolysate stage (HS) (24.37%) was significantly higher than that of GS (4.89%) and AS (11.93%). And the analysis at the genus level showed the presence of a large number of genera of organic matter hydrolysis and acid production in HS that were almost absent in other stages, such as Paludibacter (12.83%), Gracilibacteria (4.27%), f__Prolixibacteraceae_Unclassified (2.94%). In addition, the higher fatty acid metabolism and lower sugar metabolism of HS during carbon metabolism were similar to the ratio of AS, suggesting that CCH was mainly fermented to acids and then involved in the tricarboxylic acid (TCA) cycle. During nitrogen metabolism, the high relative abundance of narG, nirS, and nosZ ensured the denitrification process. The results of this study were expected to provide a theoretical basis and data support for promoting denitrification from novel carbon sources.

H2O2-assisted detection of melamine using fluorescent probe based on corn cob carbon dots-Ionic Liquid-Silver nanoparticles.

In this work, a new sustainable melamine detection method was developed. Biomass-derived carbon dots (CDs) were successfully prepared by ionic liquid (1-Allyl-3-methylimidazolium chloride, IL) hydrothermal method using agricultural waste corn cob as the carbon source, and combined with silver nanoparticles (AgNPs) to construct fluorescent probe CDs@IL-AgNPs. The probe was characterized and the formation mechanism of the probe was discussed. The direct detection of H2O2 and the indirect sensitive detection of melamine were realized. The detection limit of melamine was 0.94 µmol/L, which is far lower than the minimum allowable amount of melamine in milk powder (7.95 µmol/L). The high sensitivity and selectivity probe was used to detect melamine in commercial dairy products, and the recovery rate of standard addition was between 94 % and 110 %. This study provides valuable new application ideas for the detection of melamine in dairy products and the low-carbon and environmentally friendly treatment of agricultural waste.

Enhanced biodegradation of oil-contaminated soil oil in shale gas exploitation by biochar immobilization.

The enhanced biodegradation of oil-contaminated soil by fixing microorganisms with corn cob biochar was investigated. It was found that the components of oil in the test soil were mainly straight-chain alkanes and branched alkanes. When using corn cob biochar as a carrier to immobilize microorganisms, the best particle size of corn cob biochar as an immobilization carrier was 0.08 mm, and the best immobilization time was 18 h. SEM analysis confirmed that the microorganisms were immobilized on the corn cob biochar. Immobilized microorganisms exhibited high biodegradability under stress to high concentrations of petroleum pollutants, heavy metals, and organic pollutants. Infrared spectroscopy analysis showed that oxygen-containing groups such as hydroxyl, carboxyl, and methoxy on the surface of biochar were involved in the complexation of heavy metals. The mechanism of immobilization promoted microbial degradation of oil contamination was explained by gas chromatography mass. First, alkanes and aromatics were adsorbed by corn cob biochar and passed to immobilized microorganisms to promote their degradation. Their bioavailability increased, especially for aromatics. Second, biochar provided a more suitable environment for microorganisms to degrade. Third, the conversion of ketones to acids was accelerated during the biodegradation of alkanes, and the biodegradation of alkanes was accelerated by immobilization. The biodegradable efficiency of oil by immobilized microorganisms in soil was 70.10% within 60 days, 28.80% higher than that of free microorganisms. The degradation of immobilized microorganisms was highly correlated with the activities of catalase, urease, and polyphenol oxidase.