Equilibrium Moisture Mediated Esterification Reaction to Achieve Over 100% Lignocellulosic Nanofibrils Yield.

Lignocellulosic nanofibrils (LCNFs) isolation is recognized as an efficient strategy for maximizing biomass utilization. Nevertheless, achieving a 100% yield presents a formidable challenge. Here, an esterification strategy mediated by the equilibrium moisture in biomass is proposed for LCNFs preparation without the use of catalysts, resulting in a yield exceeding 100%. Different from anhydrous chemical thermomechanical pulp (CTMP0%), the presence of moisture (moisture content of 7 wt%, denoted as CTMP7%) introduces a notably distinct process for the pretreatment of CTMP, comprising the initial disintegration and the post-esterification steps. The maleic acid, generated through maleic anhydride (MA) hydrolysis, degrades the recalcitrant lignin-carbohydrate complex (LCC) structures, resulting in esterified CTMP7% (E-CTMP7%). The highly grafted esters compensate for the mass loss resulting from the partial removal of hydrolyzed lignin and hemicellulose, ensuring a high yield. Following microfluidization, favorable LCNF7% with a high yield (114.4 ± 3.0%) and a high charge content (1.74 ± 0.09 mmol g-1) can be easily produced, surpassing most previous records for LCNFs. Additionally, LCNF7% presented highly processability for filaments, films, and 3D honeycomb structures preparation. These findings provide valuable insights and guidance for achieving a high yield in the isolation of LCNFs from biomass through the mediation of equilibrium moisture.

Translation Notes: Heidegger, Derrida, and the Chance for (a) Philosophy.

This paper aims to show that translation is not only a fully-fledged philosophical problem, but also a specific philosophical praxis and a test bed for extracting the core of different philosophical frameworks. For this purpose, I will take into consideration the respective philosophies of Martin Heidegger and Jacques Derrida. Even if Heidegger often practices translation from the Greek in his own works and adds a few remarks towards an original investigation of this activity, he ultimately understands translation as a 'makeshift' or as a 'shipwreck'. Throughout his contestation of Heidegger's position, Derrida shows the trap of the endless appropriation of the experience of the origin structure. He also frees up the discourse by putting the hierarchical polarization between the original and the translation into question. Thus, translation becomes a chance for philosophy, even for Derrida's deconstruction, a chance to generate new paths for investigation and to keep its question open.

Gut microbiome-derived hydrolases-an underrated target of natural product metabolism.

In recent years, there has been increasing interest in studying gut microbiome-derived hydrolases in relation to oral drug metabolism, particularly focusing on natural product drugs. Despite the significance of natural product drugs in the field of oral medications, there is a lack of research on the regulatory interplay between gut microbiome-derived hydrolases and these drugs. This review delves into the interaction between intestinal microbiome-derived hydrolases and natural product drugs metabolism from three key perspectives. Firstly, it examines the impact of glycoside hydrolases, amide hydrolases, carboxylesterase, bile salt hydrolases, and epoxide hydrolase on the structure of natural products. Secondly, it explores how natural product drugs influence microbiome-derived hydrolases. Lastly, it analyzes the impact of interactions between hydrolases and natural products on disease development and the challenges in developing microbial-derived enzymes. The overarching goal of this review is to lay a solid theoretical foundation for the advancement of research and development in new natural product drugs and personalized treatment.

Enhanced fermentation and deconstruction of natural wheat straw by Trichoderma asperellum T-1 and its positive transcriptional response.

Microorganisms harvest energy from agricultural waste by degrading its structure. By comparing with Trichoderma reesei QM6a in cellulase production, straw deconstruction and transcriptome response, Trichoderma asperellum T-1 was identified to be prioritized for the fermentation of natural straw. Cellulase activity of T-1 was 50%-102% higher than QM6a. And the degradation rate of hemicellulose and ligin in wheat straw by T-1 reached 40% and 42%. Time-driven changes in the gene expression of extracellular proteins involved in polysaccharide, xylan, and hemicellulose metabolism and hydrolysis indicated that T-1 positively responded in both solid state fermentation and submerged fermentation for lignocellulose degradation. A significantly enriched category encoding carbohydrate-binding modules is considered critical for the deconstruction of the natural structure by T-1. The findings highlight the superiority of T. asperellum T-1 in straw fermentation, base on which, the construction of efficient microbial agents is expected to enhance the utilization of biomass.

As teorias pós-modernas: os princípios e o método da desconstrução generalizada / Théories postmodernes: principes et méthode de déconstruction généralisée / Postmodern theories: the principles and method of generalized deconstruction

Este artigo faz parte de um projeto de trabalho sobre as relações entre feminismo e patriarcado na pós-modernidade. Parto da seguinte questão: qual é o mal-estar que se apresenta nas teorias feministas do século XXI e de inspiração pós-moderna? O mal-estar no feminismo ainda é sexual, como evidenciam as teorias que discutem as relações entre os sexos como a matriz da dominação masculina sobre a mulher? Como passo preliminar a esta análise, faz-se necessário apresentar as ferramentas teóricas que me permitem examinar o enlace entre as teorias feministas e as teorias pós-modernas. Desse modo, neste artigo, pretendo elencar os principais elementos teóricos das chamadas teorias críticas. Estas são a base da ideologia multiculturalista pós-moderna e constituem uma referência dominante da literatura feminista no século XXI. As teorias críticas se desenvolveram a partir do eco da descrença, da decepção, da destituição do projeto da modernidade científica da sociedade ocidental. Estas teorias estruturam-se em dois eixos fundamentais: um ceticismo radical em relação ao conhecimento objetivo, científico e a primazia das relações de poder como a força constituinte da sociedade. Dessa perspectiva, constitui-se um método da desconstrução generalizada de todas as referências simbólicas, culturais da sociedade ocidental, consideradas artifícios de dominação da elite sobre as minorias subalternas, dominadas. Como eixos desse método, destaco os seguintes pontos: o locus privilegiado do campo de ação do método de desconstrução é o campo da linguagem e os seus principais instrumentos são o chamado lugar de fala e o movimento politicamente correto.

Cet article s'inscrit dans le cadre d'un projet de travail sur les relations entre féminisme et patriarcat dans la postmodernité. Je commence par la question suivante : quel est le malaise qui apparaît dans les théories féministes du XXIe siècle et d'inspiration postmoderne ? Le malaise du féminisme est-il toujours sexuel, comme en témoignent les théories qui discutent des relations entre les sexes comme matrice de la domination masculine sur les femmes ? En guise d'étape préliminaire à cette analyse, il est nécessaire de présenter les outils théoriques qui me permettent d'examiner le lien entre les théories féministes et les théories postmodernes. C'est pourquoi, dans cet article, j'ai l'intention de lister les principaux éléments théoriques des théories dites critiques. Celles-ci constituent la base de l'idéologie multiculturaliste postmoderne et constituent une référence dominante dans la littérature féministe du XXIe siècle. Les théories critiques se sont développées à partir de l'écho de l'incrédulité, de la déception et du rejet du projet de modernité scientifique dans la société occidentale. Ces théories se structurent autour de deux axes fondamentaux : un scepticisme radical à l'égard des connaissances objectives et scientifiques et la primauté des relations de pouvoir comme force constitutive de la société. De ce point de vue, il constitue une méthode de déconstruction généralisée de toutes les références symboliques et culturelles de la société occidentale, considérées comme des dispositifs de domination des élites sur des minorités subordonnées et dominées. Comme axes de cette méthode, je souligne les points suivants : le lieu privilégié du champ d'action de la méthode de déconstruction est le champ du langage et ses principaux instruments sont ce qu'on appelle le lieu de parole et le mouvement politiquement correct.

This article is part of a work project on the relationship between feminism and patriarchy in postmodernity. I start with the following question: what is the malaise that appears in 21st century and postmodern-inspired feminist theories? Is the malaise in feminism still sexual, as evidenced by theories that discuss relations between the sexes as the matrix of male domination over women? As a preliminary step to this analysis, it is necessary to present the theoretical tools that allow me to examine the link between feminist theories and postmodern theories. Therefore, in this article, I intend to list the main theoretical elements of the so-called critical theories. These are the basis of postmodern multiculturalist ideology and constitute a dominant reference in feminist literature in the 21st century. Critical theories developed from the echo of disbelief, disappointment, and dismissal of the project of scientific modernity in Western society. These theories are structured around two fundamental axes: a radical skepticism in relation to objective, scientific knowledge and the primacy of power relations as the constituent force of society. From this perspective, it constitutes a method of generalized deconstruction of all symbolic and cultural references of Western society, considered devices of elite domination over subordinate, dominated minorities. As axes of this method, I highlight the following points: the privileged locus of the field of action of the deconstruction method is the field of language and its main instruments are the so-called place of speech and the politically correct movement.

Investigation of chemical, physical and morpho-mechanical properties of banana-plantain stalk fibers for ropes and woven fabrics used in composite and limited-lifespan geotextile.

This study aimed to assess the potential of banana-plantain stalk fibers (BPSF) as a raw material for ropes and fabrics used in composites and geotextiles. Fibers were obtained by Biological retting and ropes used for geotextile weaving were obtained by three-strand twisting in order to optimize the mechanical properties of geostalk. The thermal, physical, chemical and mechanical characteristics of the fibers were studied in order to assess the impact of the extraction process on fiber performance. In addition, the microstructure of fibers and ropes was analyzed using Scanning Electron Microscopy (SEM) and the results highlighted the presence of cellulose microfibrils parallel to fiber axis and hemicellulose linked by lignin matrix. These constituents are organized in three concentric layers around the lumen. Elementary chemical analyses using X-ray energy dispersion (EDS), Fourier Transform Infrared (FTIR) and chemical deconstruction using Jayme-Wise protocol were carried out to determine the chemical composition of BPSF, which consists of 51.5 % Carbon, 47.07 % Oxygen and mineral salts that can be highly contribute to soil fertilization after degradation. These chemical constituents represent 40 % cellulose, 21.5 % hemicellulose, 24 % lignin, 0.34 % pectin, 7.2 % lip soluble extractable and 7.36 % water-soluble sugars present in BPSF. Thermal properties of BPSF have been investigated showing the initial degradation around 200 °C. Physical analysis and uniaxial tensile testing were performed to determine the multi-scale physical and mechanical properties of geostalk. Statistical evaluation using Weibull distribution established an increasing rate of physical and mechanical properties from the finest scale to the macroscopic scale. Thus, from the BPSF to the ropes, titer increases from 42.5 ± 4.5 g/km to 7983.4 ± 132 g/km and elongation at break increases from 0.75 ± 0.29 mm for the fibers to 52.42 ± 18.91 mm for geostalk. With mass per unit area of 1869 g/m2, the tensile stress of 1281.05 ± 273 MPa and maximum strength of 15.4 ± 1.74 kN/m, geostalk is a sustainable woven fabric alternative to geosynthetics for soil reinforcement as other limited lifespan geotextiles (geojute, geocoir and geosisal). In addition, the thermal stability and high mechanical properties of fibers and ropes suggest their potential application as reinforced phases in composite materials.

Amazons in Mali? Women's experiences of breast cancer and gender (re)negotiation.

Breast cancer is the second most common cancer, with more than 2.31 million cases diagnosed worldwide in 2022. Cancer medicine subjects the body to invasive procedures in the hope of offering a chance of recovery. In the course of treatment, the body is pricked, burned, incised and amputated, sometimes shattering identity and often changing the way women perceive the world. In sub-Saharan Africa, incidence rates are steadily increasing and women are particularly young when they develop breast cancer. Despite this alarming situation, the scientific literature on breast cancer in sub-Saharan Africa is poor and largely dominated by medical literature. Using a qualitative approach and a theoretical framework at the intersection of the sociology of gender and the sociology of the body, we explore the discourse strategies of women with breast cancer in Mali regarding their relationship to the body and to others. Based on 25 semi-directive interviews, we analyse the experiences of these women. Using the image of the Amazon woman, whose struggle has challenged gender because of its masculine attributes, we explore whether these women's fight against their breast cancer could be an opportunity to renegotiate gender relations. The experience of these women is characterised by the deconstruction of their bodies, pain and suffering. The masculinisation of their bodies and their inability to perform certain typically female functions in society (such as cooking or sexuality) challenges their female identity. The resistance observed through the sorority, discreet mobilisation and display of their bodies does not seem to be part of a renegotiation of gender relations, but it does play an active role in women's acceptance of the disease and their reconstruction.

Engineering the cellulolytic bacterium, Clostridium thermocellum, to co-utilize hemicellulose.

Consolidated bioprocessing (CBP) of lignocellulosic biomass holds promise to realize economic production of second-generation biofuels/chemicals, and Clostridium thermocellum is a leading candidate for CBP due to it being one of the fastest degraders of crystalline cellulose and lignocellulosic biomass. However, CBP by C. thermocellum is approached with co-cultures, because C. thermocellum does not utilize hemicellulose. When compared with a single-species fermentation, the co-culture system introduces unnecessary process complexity that may compromise process robustness. In this study, we engineered C. thermocellum to co-utilize hemicellulose without the need for co-culture. By evolving our previously engineered xylose-utilizing strain in xylose, an evolved clonal isolate (KJC19-9) was obtained and showed improved specific growth rate on xylose by ∼3-fold and displayed comparable growth to a minimally engineered strain grown on the bacteria's naturally preferred substrate, cellobiose. To enable full xylan deconstruction to xylose, we recombinantly expressed three different ß-xylosidase enzymes originating from Thermoanaerobacterium saccharolyticum into KJC19-9 and demonstrated growth on xylan with one of the enzymes. This recombinant strain was capable of co-utilizing cellulose and xylan simultaneously, and we integrated the ß-xylosidase gene into the KJC19-9 genome, creating the KJCBXint strain. The strain, KJC19-9, consumed monomeric xylose but accumulated xylobiose when grown on pretreated corn stover, whereas the final KJCBXint strain showed significantly greater deconstruction of xylan and xylobiose. This is the first reported C. thermocellum strain capable of degrading and assimilating hemicellulose polysaccharide while retaining its cellulolytic capabilities, unlocking significant potential for CBP in advancing the bioeconomy.

Comparison of Peyton's Four-Step Approach With the Conventional Bedside Technique in Teaching Clinical Examination Skills to Medical Students.

BACKGROUND:  Conventional bedside teaching (CBT) is an integral and classical method for imparting clinical skills to undergraduates in medical schools. It is a traditionally successful approach, especially when it comes to imparting patient-doctor relationship skills and knowledge on clinical management. Peyton's four-step approach (PFSA) is one of the newer structured instructional approaches for teaching-learning, especially for imparting procedural and complex psychomotor skills. The present study compares the application of PFSA in teaching complex systemic examination skills to the CBT technique in teaching the same skill to MBBS students. The impact of the acquisition of this examination skill was assessed statistically to compare PFSA and CBT methodologies. METHODOLOGY:  The target population was MBBS (Bachelor of Medicine and Bachelor of Surgery) students; for this study, the phase II MBBS students were considered as the study population since they were relatively naïve to clinical bedside examination skills. Students were allotted groups and they were taught clinical skills through CBT and PFSA separately. Using the OpenEpi toolkit Version 3 open-source sample size calculator for comparing two means, the sample size was 30 students in each group. The students were assessed for their competency and were also made to fill out a feedback questionnaire to compare the two methods of education dispensing.  Results: The results of this study showed that PFSA is definitely suitable for teaching clinical examination skills. The acquisition of skills was found non-inferior to CBT while the retention of these skills was found to be equally good or even superior with PFSA than with CBT.  Conclusion: PFSA has already been proven to be a good teaching method for the acquisition of complex procedural skills. This study expands the role of PFSA in teaching clinical bedside examination skills to medical students. Further large-scale studies may clarify the learning impact and outcomes of PFSA in clinical bedside teaching.

The 3-Dimensional Temporal Bone Dissection Manual: Operable Stepwise Models for Teaching Otologic Surgery.

Deconstructing surgeries into steps and providing instructions with illustrations has been the staple of surgical textbooks for decades. However, it may be difficult for the novice surgeon to interpret 2-dimensional (2D) illustrations into 3D surgeries. The objective of this study is to create operable models that demonstrate the progression of surgery in 3D and allow for mastering the final steps of the operation first. Mastoidectomy was performed in a stepwise fashion to different end points on 5 identical 3D-printed temporal bone models to represent 5 major steps of the operation. The drilled models were computed tomography scanned and the subsequent images were used to create 3D model copies of each step. This is the first study to demonstrate that it is possible to create, scan, and copy stepwise, operable, patient-specific 3D-printed models, which the trainee can both reference as a 3D dissection guide and can operate on repeatedly and in any order.

Targeted rRNA depletion enables efficient mRNA sequencing in diverse bacterial species and complex co-cultures.

IMPORTANCE: Microbes present one of the most diverse sources of biochemistry in nature, and mRNA sequencing provides a comprehensive view of this biological activity by quantitatively measuring microbial transcriptomes. However, efficient mRNA capture for sequencing presents significant challenges in prokaryotes as mRNAs are not poly-adenylated and typically make up less than 5% of total RNA compared with rRNAs that exceed 80%. Recently developed methods for sequencing bacterial mRNA typically rely on depleting rRNA by tiling large probe sets against rRNAs; however, such approaches are expensive, time-consuming, and challenging to scale to varied bacterial species and complex microbial communities. Therefore, we developed EMBR-seq+, a method that requires fewer than 10 short oligonucleotides per rRNA to achieve up to 99% rRNA depletion in diverse bacterial species. Finally, EMBR-seq+ resulted in a deeper view of the transcriptome, enabling systematic quantification of how microbial interactions result in altering the transcriptional state of bacteria within co-cultures.

Characterization of lignin and hemicellulose degrading bacteria isolated from cow rumen and forest soil: Unveiling a novel enzymatic model for rice straw deconstruction.

Application of greener pretreatment technology using robust ligninolytic bacteria for short duration to deconstruct rice straw and enhance bioethanol production is currently lacking. The objective of this study is to characterize three bacterial strains isolated from the milieux of cow rumen and forest soil and explore their capabilities of breaking down lignocellulose - an essential process in bioethanol production. Using biochemical and genomic analyses these strains were identified as Bacillus sp. HSTU-bmb18, Bacillus sp. HSTU-bmb19, and Citrobacter sp. HSTU-bmb20. Genomic analysis of the strains unveiled validated model hemicellulases, multicopper oxidases, and pectate lyases. These enzymes exhibited interactions with distinct lignocellulose substrates, further affirmed by their stability in molecular dynamic simulations. A comprehensive expression of ligninolytic pathways, including ß-ketoadipate, phenyl acetate, and benzoate, was observed within the HSTU-bmb20 genome. The strains secreted approximately 75-82 U/mL of cellulase, xylase, pectinase, and lignin peroxidase. FT-IR analysis of the bacterial treated rice straw fibers revealed that the intensity of lignin-related peaks decreased, while cellulose-related peaks sharpened. The values of crystallinity index for the untreated control and the treated rice straw with either HSTU-bmb18, or HSTU-bmb19, or HSTU-bmb20 were recorded to be 34.48, 28.49, 29.36, 31.75, respectively, which are much higher than that of 13.53 noted for those treated with the bacterial consortium. The ratio of fermentable cellulose in rice straw increased by 1.25-, 1.79-, 1.93- and 2.17-fold following treatments with HSTU-bmb18, HSTU-bmb20, HSTU-bmb19, and a mixed consortium of these three strains, respectively. These aggregative results suggested a novel model for rice straw deconstruction utilizing hydrolytic enzymes of the consortium, revealing superior efficacy compared to individual strains, and advancing cost-effective, affordable, and sustainable green technology.

Transdisciplinary pragmatic melioration for the plastic life cycle: Why the social, natural, and technical sciences should prioritize reducing harm.

Plastics underpin modern society but also threaten to choke it. Only 9 % of all plastic waste is recycled, usually with loss of quality ("downcycling"); the rest is landfilled or dumped (79 %) or incinerated (12 %). Put bluntly, the "plastic age" needs a "sustainable plastic culture." Consequently, we urgently need to develop a global and transdisciplinary approach not only to fully recycle plastics but also to manage the harms across their life cycle. The past decade has witnessed an explosion in research on new technologies and interventions that purport to help solve the plastic waste challenge; however, this work has, in most cases, been carried forward within single disciplines (for example, researching novel chemical and bio-based technologies for plastic degradation, engineering processing equipment innovations, and mapping recycling behaviours). In particular, although there has been vast progress within individual scientific fields, such work does not address the complexities of various plastic types and waste management systems. Meanwhile, research on the social contexts (and constraints) of plastic use and disposal is rarely in conversation with the sciences to drive innovation. In short, research on plastics typically lacks a transdisciplinary perspective. In this review, we urge the adoption of a transdisciplinary approach that focuses on pragmatic melioration; such an approach combines the natural and technical sciences with the social sciences to focus on the mitigation of harms across the plastic life cycle. To illustrate our case, we review the status of plastic recycling from these three scientific perspectives. Based on this, we advocate 1) foundational studies to identify sources of harm and 2) global/local interventions aimed at those plastics and aspects of the plastic life cycle that cause maximal harm, both in terms of planetary welfare and social justice. We believe this approach to plastic stewardship can be a showcase for tackling other environmental challenges.

Deconstructed Plastic Substrate Preferences of Microbial Populations from the Natural Environment.

Over half of the world's plastic waste is landfilled, where it is estimated to take hundreds of years to degrade. Given the continued use and disposal of plastic products, it is vital that we develop fast and effective ways to utilize plastic waste. Here, we explore the potential of tandem chemical and biological processing to process various plastics quickly and effectively. Four samples of compost or sediment were used to set up enrichment cultures grown on mixtures of compounds, including disodium terephthalate and terephthalic acid (monomers of polyethylene terephthalate), compounds derived from the chemical deconstruction of polycarbonate, and pyrolysis oil derived from high-density polyethylene plastics. Established enrichment communities were also grown on individual substrates to investigate the substrate preferences of different taxa. Biomass harvested from the cultures was characterized using 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing. These data reveal low-diversity microbial communities structured by differences in culture inoculum, culture substrate source plastic type, and time. Microbial populations from the classes Alphaproteobacteria, Gammaproteobacteria, Actinobacteria, and Acidobacteriae were significantly enriched when grown on substrates derived from high-density polyethylene and polycarbonate. The metagenomic data contain abundant aromatic and aliphatic hydrocarbon degradation genes relevant to the biodegradation of deconstructed plastic substrates used here. We show that microbial populations from diverse environments are capable of growth on substrates derived from the chemical deconstruction or pyrolysis of multiple plastic types and that paired chemical and biological processing of plastics should be further developed for industrial applications to manage plastic waste. IMPORTANCE The durability and impermeable nature of plastics have made them a popular material for numerous applications, but these same qualities make plastics difficult to dispose of, resulting in massive amounts of accumulated plastic waste in landfills and the natural environment. Since plastic use and disposal are projected to increase in the future, novel methods to effectively break down and dispose of current and future plastic waste are desperately needed. We show that the products of chemical deconstruction or pyrolysis of plastic can successfully sustain the growth of low-diversity microbial communities. These communities were enriched from multiple environmental sources and are capable of degrading complex xenobiotic carbon compounds. This study demonstrates that tandem chemical and biological processing can be used to degrade multiple types of plastics over a relatively short period of time and may be a future avenue for the mitigation of rapidly accumulating plastic waste.

Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate).

Upcycling processes via tandem chemical deconstruction and biological transformation has shown promise for poly(ethylene terephthalate) (PET) waste open-loop management. Under this framework, postconsumer PET becomes a low-cost and abundant starting material for the synthesis of high-value chemicals.

The shiny new object: Deconstructing the patient-oriented paradigm in health sciences.

RATIONALE: A 'patient-oriented' research paradigm, also known as patient and public engagement, has infiltrated the field of health sciences and continues to spread. At first blush, it is difficult to reprove anything labelled 'patient-oriented'; however, the patient-oriented paradigm may easily become an ideological 'good', leading to unintended consequences that may well prove more detrimental than beneficial. While patient-oriented research has its roots in more radical forms of patient and public engagement, its recent instantiation betrays its roots and forecloses on more radical forms of engagement, such as critical participatory research. AIM AND OBJECTIVES: The objective of this article is to deconstruct the patient-oriented research narrative and to demonstrate how such a discourse imposes itself as a dominant approach in health sciences. APPROACH: Following Derrida's deconstructive approach, we bring to light the unexamined presuppositions, false pretences, and presumed 'goodness' and 'naturalness' of patient-oriented discourse. DISCUSSION: By deconstructing the patient-oriented narrative we demonstrate how pre-existing power structures (biomedical, economic, etc.) shape the conduct of the approach and serve to depoliticize the truly participatory aspects of research. Rather than being modelled on the evidence-based movement or seen as its natural 'evolution', patient-oriented research should resist by affirming itself as a radical form that is both participatory and emancipatory.

Effectiveness of four deconstructive meditative practices on well-being and self-deconstruction: study protocol for an exploratory randomized controlled trial.

INTRODUCTION: The efficacy of interventions based on mindfulness and compassion has been demonstrated in both clinical and general population, and in different social contexts. These interventions include so-called attentional and constructive meditation practices, respectively. However, there is a third group, known as deconstructive meditation practices, which has not been scientifically studied. Deconstructive practices aim to undo maladaptive cognitive patterns and generate knowledge about internal models of oneself, others and the world. Although there are theoretical and philosophical studies on the origin of addiction to the self or on the mechanisms of action associated with the deconstruction of the self, there are no randomized controlled trials evaluating these techniques in either a healthy population or clinical samples. This study aims to evaluate the effect of three deconstructive techniques by comparing them to mindfulness in the general population. METHODS AND ANALYSIS: A randomized controlled clinical trial will be conducted with about 240 participants allocated to four groups: (a) mindful breathing, (b) prostrations, according to Tibetan Buddhist tradition; (c) the Koan Mu, according to Zen Buddhist tradition; and (d) the mirror exercise, according to Toltec tradition. The primary outcome will be the qualities of the non-dual experience and spiritual awakening, measured by the Nondual Embodiment Thematic Inventory, assessed at pre- and post-treatment and at 3- and 6-month follow-ups. Other outcomes will be mindfulness, happiness, compassion, affectivity and altered state of consciousness. Quantitative data will be compared using mixed-effects linear regression models, and qualitative data will be analysed through thematic analysis and using the constant comparative method from grounded theory. ETHICS AND DISSEMINATION: Approval was obtained from the Research Ethics Committee of Aragon, Spain. The results will be submitted to peer-reviewed specialized journals, and brief reports will be sent to participants on request. TRIAL REGISTRATION: ClinicalTrials.gov NCT05317754. Registered on August 2,2022.

BsLPMO10A from Bacillus subtilis boosts the depolymerization of diverse polysaccharides linked via ß-1,4-glycosidic bonds.

Lytic polysaccharide monooxygenase (LPMO) is known as an oxidatively cleaving enzyme in recalcitrant polysaccharide deconstruction. Herein, we report a novel AA10 LPMO derived from Bacillus subtilis (BsLPMO10A). A substrate specificity study revealed that the enzyme exhibited an extensive active-substrate spectrum, particularly for polysaccharides linked via ß-1,4 glycosidic bonds, such as ß-(Man1 â†’ 4Man), ß-(Glc1 â†’ 4Glc) and ß-(Xyl1 â†’ 4Xyl). HPAEC-PAD and MALDI-TOF-MS analyses indicated that BsLPMO10A dominantly liberated native oligosaccharides with a degree of polymerization (DP) of 3-6 and C1-oxidized oligosaccharides ranging from DP3ox to DP6ox from mixed linkage glucans and beechwood xylan. Due to its synergistic action with a variety of glycoside hydrolases, including glucanase IDSGLUC5-38, xylanase TfXYN11-1, cellulase IDSGLUC5-11 and chitinase BtCHI18-1, BsLPMO10A dramatically accelerated glucan, xylan, cellulose and chitin saccharification. After co-reaction for 72 h, the reducing sugars in Icelandic moss lichenan, beechwood xylan, phosphoric acid swollen cellulose and chitin yielded 3176 ± 97, 7436 ± 165, 649 ± 44, and 2604 ± 130 µmol/L, which were 1.47-, 1.56-, 1.44- and 1.25-fold higher than those in the GHs alone groups, respectively (P < 0.001). In addition, the synergy of BsLPMO10A and GHs was further validated by the degradation of natural feedstuffs, the co-operation of BsLPMO10A and GHs released 3266 ± 182 and 1725 ± 107 µmol/L of reducing sugars from Oryza sativa L. and Arachis hypogaea L. straws, respectively, which were significantly higher than those produced by GHs alone (P < 0.001). Furthermore, BsLPMO10A also accelerated the liberation of reducing sugars from Celluclast® 1.5 L, a commercial cellulase cocktail, on filter paper, A. hypogaea L. and O. sativa L. straws by 49.58 % (P < 0.05), 72.19 % (P < 0.001) and 54.36 % (P < 0.05), respectively. This work has characterized BsLPMO10A with a broad active-substrate scope, providing a promising candidate for lignocellulosic biomass biorefinery.

From domestication to imperial patronage: Deconstructing the biomedicalisation of occupational therapy.

Occupational therapy knowledge emerged in the 19th century as reformist movements responded to the industrialisation of society and capitalist expansion. In the Global North, it was institutionalised by State apparatuses during the First and Second World Wars. Although biomedicine contributed to the rapid expansion and establishment of occupational therapy as a health discipline, its domestication by the biomedical model led to an overly regulated profession that betrays its reformist ideals. Drawing on the work of Deleuze and Guattari, our aim in this article is to deconstruct the biomedicalisation of occupational therapy and demonstrate how resistance to this process is critical for the future of this discipline. The use of arts and crafts in occupational therapy may be conceptualised as a 'nomad science' aesthetically resisting the domination of industrialism and medical reductionism. Through the war efforts, a coalition of progressive nurses, social workers, teachers, artisans and activists metamorphosed into occupational therapists. As it did with nursing, biomedicine proceeded to domesticate occupational therapy through a form of 'imperial' patronage subsequently embodied in the evidence-based movement. 'Occupational' jargon is widely used today and may be viewed as the product of a profession trying to establish itself as an autonomous discipline that imposes its own regime of truth. Given the symbolic violence underlying this patronage, the future of occupational therapy should not mean behaving according to biomedicine's terms. As a discipline, occupational therapy must resist the appropriation of its 'war machine' and craft its own terms through the release of new creative energy.

Insights into key factors affecting bioconversion efficiency of rattan biomass: The supramolecular structural variations of cellulose.

Global energy concerns urged us to search for sufficient utilization of biomass to renewable energy. Herein, rattan biomass displaying herbaceous species-like anatomy and hardwood-like chemical composition was used as model of lignocellulose to determine its recalcitrance inhibiting efficient bioconversion. Delignification and continuous mild alkaline treatments were applied for deconstruction of rattan cane (Calamus simplicifolius) followed by cellulase enzymatic hydrolysis. Cellulose supramolecular structural variations were proved to be the major reason for the enhanced hydrolysis in addition to the removal of lignin and hemicelluloses matrix. Lowered crystallinity (50-65 %) as well as swelled crystallite sizes (4.8-5.0 nm) during allomorphic transformation favored the enhanced hydrolysis, rather than the crystalline cellulose II. Moreover, well-distributed separation and fibrillation of cellulose elementary fibrils also contributed to glucose yield promotion. The study will provide new insights to the strategy to efficient bioconversion of lignocellulosic biomass.