ABSTRACT
INTRODUCTION: It has been reported that microbiological diagnosis of Cutibacterium spp. infection requires a prolonged incubation time (up to 14 days). We present our experience with regard to incubation time for detection of Cutibacterium spp. in orthopaedic samples over a 10-year period. METHODS: One hundred and nineteen samples were included in this retrospective study. Fifty-three were implants (having previously undergone sonication), 64 were periprosthetic tissue biopsies and two were synovial fluids. Atkins's criteria were used for interpreting the isolates. Quantification and number of days until a culture became positive for Cutibacterium spp. were evaluated. RESULTS: The median number of days to detection of a clinically significant isolate and a contaminant was 4 days. No clinically significant isolates grew after day eight. CONCLUSION: Most clinically significant isolates of Cutibacterium spp. are detected in the first 7 days of incubation, although a recommendation of prolonged incubation (up to 14 days) appears to be necessary for detecting other organisms.
Subject(s)
Orthopedics , Propionibacteriaceae , Prosthesis-Related Infections , Humans , Prosthesis-Related Infections/diagnosis , Retrospective Studies , SonicationABSTRACT
Introduction: It has been reported that microbiological diagnosis of Cutibacterium spp. infection requires a prolonged incubation time (up to 14 days). We present our experience with regard to incubation time for detection of Cutibacterium spp. in orthopaedic samples over a 10-year period. Methods: One hundred and nineteen samples were included in this retrospective study. Fifty-three were implants (having previously undergone sonication), 64 were periprosthetic tissue biopsies and two were synovial fluids. Atkins's criteria were used for interpreting the isolates. Quantification and number of days until a culture became positive for Cutibacterium spp. were evaluated. Results: The median number of days to detection of a clinically significant isolate and a contaminant was 4 days. No clinically significant isolates grew after day eight. Conclusion: Most clinically significant isolates of Cutibacterium spp. are detected in the first 7 days of incubation, although a recommendation of prolonged incubation (up to 14 days) appears to be necessary for detecting other organisms.(AU)
Introducción: Se ha reportado que el diagnóstico microbiológico de las infecciones por Cutibacterium spp. requiere un tiempo de incubación prolongado (hasta 14 días). Presentamos nuestra experiencia al respecto en muestras ortopédicas durante un período de 10 años. Métodos: Se incluyeron en este estudio retrospectivo 119 muestras de las que 53 fueron implantes (previa sonicación), 64 biopsias de tejido periprotésico y dos líquidos sinoviales. Para la interpretación se siguieron los criterios de Atkins. Se evaluó la cuantificación y el número de días hasta que el cultivo fue positivo para Cutibacterium spp. Resultados: La mediana del número de días para detectar un aislado clínicamente significativo y un contaminante fue de cuatro días. Ningún aislado clínicamente relevante creció después del día ocho. Conclusión: La mayoría de aislados clínicamente significativos de Cutibacterium spp. se detectan durante los siete primeros días de incubación, sin embargo, parece necesaria una incubación de hasta 14 días para la detección de otros microorganismos.(AU)
