Development of novel green methods for preparation of lead-free preserved pidan (duck egg).

Pidan, a pickled duck egg, is a traditional Chinese cuisine and generally produced by soaking in metal ion containing strong alkaline solution such as NaOH solution. However, nowadays consumers possess negative perception for using strong alkali in food processing. Therefore, the objective of the current study was to determine the potential of incinerated eggshell powder and alkaline electrolyzed oxidized (EO) water for pidan production rather than harmful NaOH use. This study aims to obtain the optimal physicochemical and sensory qualities of pidan. Various dosing (1-5%) of the incinerated eggshell powder solution or alkaline EO water was used as a basic pickling solution. Duck eggs were pickled at 25-27 °C for 15-30 days with 3 days of an observation interval. Actual commercial process commonly undergoes for 14 days of ripening, after 25 days of picking process with incinerated eggshell powder or EO water. Results showed that physicochemical and sensory attributes of pidan obtained by incinerated eggshell powder solution and alkaline EO water were not significantly different (P < 0.05) from the commercial product. This study reports a cost-effective and green alternative method for pidan processing by replacing costly NaOH without compromising their physico-chemical and sensory attributes.

Bactericidal Effect of Electrolyzed Oxidized Water Generated by WATRIX(R)-10G against Clinical Isolates / 병원감염관리

Background: Disinfection is essential for the prevention of hospital infection. The electrolyzed oxidized water generated by WATRIX(R)-10G(Microbank co., Korea) was recently developed as a disinfectant. This is not costly and does not cause any clinical problems and environmental pollution. We evaluated bactericidal activity of the electrolyzed oxidized water generated by WATRIX(R)-10G against several clinical isolates of bacteria and yeasts. Methods: 23 strains of bacteria and four strains of yeast were exposed to the electrolyzed oxidized water(40&50ppm HOel) generated by WATRIX(R)-10G for the various periods (0.5, 1, 2, 4, 10, 15, 30, 60minutes). After the exposure to electrolyzed oxidized water, 0.01mL of mixture of microorganisms and electrolyzed oxidized water was inoculated into brain-heart infusion broth or onto Sabouraud dextrose agar and incubated at 35degrees C for 48 hours. Results: All strains of bacteria and yeast were killed within 30 seconds after an exposure to 40 ppm of electrolyzed oxidized water. All vegetative forms of Bacillus subtills were killed within 10, 4 and 2 minutes in 30, 40 and 50 ppm of electrolyzed oxidized water. respectively. But, all spore forms of Bacillus subtilis were killed within 30, 15 and 10 minutes in 30, 40 and 50 ppm of electrolyzed oxidized water respectively. Conclusions: This study showed that the electrolyzed oxidized water generated by WATRIX(R)-10G was effective not only for the disinfection of commonly isolated bacteria and yeast from hospital but also against spore-forming bacteria. It may be recommended that WATRIX(R)-10G should be used for the effective disinfection of skin, instruments ,hospital floors and hand-washing.

Bactericidal Effect of Electrolyzed Oxidized Water Generated by WATRIX(R)-10G against Clinical Isolates / 병원감염관리

Background: Disinfection is essential for the prevention of hospital infection. The electrolyzed oxidized water generated by WATRIX(R)-10G(Microbank co., Korea) was recently developed as a disinfectant. This is not costly and does not cause any clinical problems and environmental pollution. We evaluated bactericidal activity of the electrolyzed oxidized water generated by WATRIX(R)-10G against several clinical isolates of bacteria and yeasts. Methods: 23 strains of bacteria and four strains of yeast were exposed to the electrolyzed oxidized water(40&50ppm HOel) generated by WATRIX(R)-10G for the various periods (0.5, 1, 2, 4, 10, 15, 30, 60minutes). After the exposure to electrolyzed oxidized water, 0.01mL of mixture of microorganisms and electrolyzed oxidized water was inoculated into brain-heart infusion broth or onto Sabouraud dextrose agar and incubated at 35degrees C for 48 hours. Results: All strains of bacteria and yeast were killed within 30 seconds after an exposure to 40 ppm of electrolyzed oxidized water. All vegetative forms of Bacillus subtills were killed within 10, 4 and 2 minutes in 30, 40 and 50 ppm of electrolyzed oxidized water. respectively. But, all spore forms of Bacillus subtilis were killed within 30, 15 and 10 minutes in 30, 40 and 50 ppm of electrolyzed oxidized water respectively. Conclusions: This study showed that the electrolyzed oxidized water generated by WATRIX(R)-10G was effective not only for the disinfection of commonly isolated bacteria and yeast from hospital but also against spore-forming bacteria. It may be recommended that WATRIX(R)-10G should be used for the effective disinfection of skin, instruments ,hospital floors and hand-washing.

Bactericidal Effect of Disinfectant HICLO-S(R) / 병원감염관리

BACKGROUND: Disinfection is essential for the prevention of hospital infection. HICLO-S (Soosan environment Co., Yongin, Korea), one of the electrolyzed oxidized water was recently developed as a disinfectant. This is not costly and does not cause any clinical problems and environmental pollution. We evaluated the disinfective activity of HICLO-S against several clinical isolates of bacteria and yeasts. METHODS: 25 strains of bacteria and two strains of yeast were exposed to HICLO-S (30 & 50 ppm HICLO-S) disinfectant for the various periods (0.5, 1, 2, 4, 8, 10, 15, 20 minutes). After the exposure to HICLO-S disinfectant, 0.01 mL of mixture of microorganisms and HICLO-S was inoculated into brain-heart infusion broth or onto Sabouraud dextrose agar and incubated at 35degrees C for 48hours. RESULTS: All strains of bacteria and yeast were killed within 30 seconds after an exposure to 30 ppm of HICLO-S. All of three strains of spore forming Bacillus subtilis were killed within 4 minutes after an exposure to 30ppm of HICLO-S, but all of three strains were killed within 30 seconds in 50 ppm of HICLO-S. CONCLUSIONS: This study showed that HICLO-S disinfectant was effective for the disinfection of commonly isolated bacteria and yeast from hospital, but less effective against spore-forming bacteria. It may be recommended that HICLO-S should he used for the effective disinfection of skin, instruments and hospital floors.