Actividad antibacteriana de un gel experimental de Eucalyptus globulus Labill frente a Porphyromonas gingivalis / Antibacterial activity of an experimental Eucalyptus globulus Labill gel against Porphyromonas gingivalis

Introducción: Las plantas medicinales han demostrado poseer propiedades antibacterianas para el control de la periodontitis. Objetivo: Determinar la actividad antibacteriana frente a Porphyromonas gingivalis ATCC 33277 de un gel experimental compuesto por aceite esencial de Eucalyptus globulus Labill. Métodos: Se realizó un estudio experimental in vitro. Se empleó el programa EPi InfoTM para el cálculo de las repeticiones. El aceite esencial se obtuvo por el método de arrastre de vapor; se identificó su composición química por cromatografía de gases acoplada a espectrometría de masas. Se evaluó la concentración mínima inhibitoria (CMI) y concentración mínima bactericida (CMB). Se realizó un ensayo de difusión en Agar para medir los halos de inhibición del gel experimental al 4,46 por ciento frente a P. gingivalis, la comparación con clorhexidina al 0,12 por ciento se evaluó con la prueba U de Mann-Whitney. Se adoptó un nivel de significancia del 5 por ciento . Resultados: Se identificaron 11 constituyentes en el aceite esencial, los principales componentes químicos fueron 3-heptadecene, (Z)- (36,13 por ciento ), 1-tridecene (14,7 por ciento ) y 1,8-cineole (9,72 por ciento ). La CMI del aceite esencial fue 36,195 mg/mL y la CMB fue 39,114 mg/mL. Los halos de inhibición del gel experimental de P. gingivalis fueron 25,533 mm ± 0,960. mm. Se observaron diferencias estadísticamente significativas frente a clorhexidina al 0,12 por ciento (23,282 ± 0,345) (p < 0,05). Conclusiones: El gel experimental al 4,46 por ciento compuesto por aceite esencial de Eucalyptus globulus Labill presentó una actividad antibacteriana importante frente a Porphyromonas gingivalis ATCC 33277(AU)

Introduction: Medicinal plants have proved to have antibacterial properties for the control of periodontitis. Objective: Determine the antibacterial activity against Porphyromonas gingivalis ATCC 33277 of an experimental gel composed of essential Eucalyptus globulus Labill oil. Methods: An in vitro experimental study was conducted. The software EPi InfoTM was used to estimate the repetitions. The essential oil was obtained by steam entrainment, and its chemical composition was determined by gas chromatography / mass spectrometry. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also evaluated. An agar diffusion test was performed to measure the inhibition haloes of the 4.46 percent experimental gel against P. gingivalis. Comparison with 0.12 percent chlorhexidine was evaluated with the Mann-Whitney U test. A 5 percent significance level was adopted. Results: A total 11 constituents were identified in the essential oil. The main chemical components were 3-Heptadecene, (Z)- (36.13 percent), 1-Tridecene (14.7 percentand 1,8-cineole (9.72 percent). MIC of the essential oil was 36.195 mg/ml, whereas MBC was 39.114 mg/ml. The inhibition haloes of the experimental P. gingivalis gel were 25.533 mm ± 0.960 mm. Statistically significant differences were observed versus 0.12 percent chlorhexidine (23.282 ± 0.345) (p < 0.05). Conclusions: The 4.46 percent experimental gel composed of Eucalyptus globulus Labill essential oil displayed considerable antibacterial activity against Porphyromonas gingivalis ATCC 33277(AU)

In situ microemulsion-gel obtained from bioadhesive hydroxypropyl methylcellulose films for transdermal administration of zidovudine.

This study aims to develop in situ microemulsion-gel (ME-Gel) obtained from hydroxypropyl methylcellulose (HPMC) films for transdermal administration of Zidovudine (AZT). Firstly, HPMC films containing propylene glycol (PG) and eucalyptus oil (EO) were obtained and characterized. Later, a pseudo-ternary phase diagram composed of water, EO, tween 80 and PG was obtained and one microemulsion (ME) with a similar proportion of the film components was obtained. ME was transformed in ME-Gel by the incorporation of HPMC. Finally, HPMC films were hydrated with Tween 80 solution to yield in situ ME-Gel and its effect on AZT skin permeation was compared with HPMC film hydrated with water (F5hyd). The results showed that the ME and ME-Gel presented a droplet size of 16.79 and 122.13 µm, respectively, polydispersity index (PDI) < 0.39 and pH between 5.10 and 5.40. The incorporation of HPMC resulted in viscosity about 2 times higher than the use of ME. The presence of AZT did not alter the formulation properties. The in situ ME-Gel promoted a two-fold increase in the permeated amount of AZT compared to F5hyd. The results suggest that it was possible to obtain an ME-Gel in situ from HPMC films and that its effect on transdermal permeation of AZT was significant.

Evaluation of the effect of coating vertex denture lining material with plant oils on microbial growth and hardness

Aims: To evaluate the effect of surface coating with natural plant oils (Salvia officinalis, ginger and eucalyptus) on Candida growth and the hardness of Vertex denture lining material. Materials and method: Forty five specimens were prepared from soft acrylic lining material, twenty five of which were 10x10x2mm in size for testing antifungal activity, and twenty samples were 20mm in diameter and 12mm in thickness, for testing shore A hardness after coating samples with three types of natural oils (Salvia officinalis, ginger and eucalyptus oils). Significant differences among the groups at (p≤0.05) level of significance were determined statistically with one way analysis of variance and Duncan's multiple range test Result: Antifungal assay showed a significant difference between five groups regarding Candida albicans growth (p≤ 0.05). For the hardness test, comparing different times of storage in water (1, 7, 14, 30 days) revealed a significant difference within all groups (p≤0. 05). While comparing the groups coated with natural oils with the control group, significant differences were found between different times of storage in water (1, 7, 30 day) (p≤0.05), except at 14 days of water storage there was no significant difference between groups (p>0.05). Conclusion: All tested natural oils were effective as fungicidal agents and increased the softness and duration of soft acrylic lining material.

Avaliação in vitro da atividade antifúngica de extratos de plantas e óleo de eucalipto sobre Trichophyton mentagrophytes / In vitro evaluation of the antifungal activity of plant extracts and eucalyptus oil on Trichophyton mentagrophytes

O presente estudo teve como objetivo determinar a ação antifúngica de extratos de plantas medicinais e óleo de eucalipto frente ao dermatófito Trichophyton mentagropytes, visando a utilização da fitoterapia no controle. As plantas utilizadas na obtenção dos extratos foram arruda (Ruta graveolens), citronela (Cymbopogon nardus), cravo de defunto (Tagetes minuta), eucalipto (Eucalyptus spp), graviola (Annona muricata), fruta do conde (Annona spp), manga (Mangifera indica), romã (Punica granatum), flores e folhas de primavera (Bougainvillea spectabilis). Verificou-se que uso de 0,5 por cento óleo de eucalipto no combate ao T. mentagropytes foi eficaz, já os extratos de citronela (4 por cento) eucalipto (5 por cento) e romã (8 por cento) atuaram como fungistáticos e os restantes não devem ser usados contra este dermatófito porque não causaram nenhum efeito.

The aim of this study was to assess the antifungal action of medicinal plant extracts and eucalyptus oil against the dermatophyte Trichophyton mentagrophytes in order to employ phytotherapy for its control. The plants used for extract production were common rue (Ruta graveolens), citronella (Cymbopogon nardus), wild marigold (Tagetes minuta), eucalyptus (Eucalyptus spp), sweetsop (Annona muricata), custard apple (Annona spp), mango (Mangifera indica), pomegranate (Punica granatum), besides flowers and leaves of bougainvillea (Bougainvillea spectabilis). The use of 0.5 percent eucalyptus oil was effective in controlling Trichophyton mentagrophytes; however, citronella (4 percent), eucalyptus (5 percent) and pomegranate (8 percent) extracts acted as fungistatic, and the remaining extracts should not be used against this dermatophyte since they did not have any effect.