ABSTRACT
AIM: To compare the potential protective effects of epi-gallocatechin gallate (EGCG) and ellagic acid (EA) in an experimental cataract model. METHODS: Twenty-eight Spraque-Dawley rat pups were assigned into four groups. All the rats, except for those in the control group, were injected subcutaneously sodium selenite to induce experimental cataract on the postpartum ninth day, and between 10th and 14th days. Rats in the sham, EGCG, and EA groups were intraperitoneally administered 50 mg/(kg·d) saline solution, 50 mg/(kg·d) EGCG and 200 mg/(kg·d) EA, respectively. The reduced glutathione (GSH) and malondialdehyde (MDA) levels, total antioxidant status (TAS) and total oxidant status (TOS) in lens supernatants were measured. RESULTS: The mean cataract gradings in EGCG and EA groups were found to be significantly lower than that in sham group (P<0.001). The mean GSH levels and TASs in EGCG and EA groups were significantly higher than that in sham group while mean MDA levels and TOSs in EGCG and EA groups were significantly lower than that in the sham group (P<0.001). CONCLUSION: EGCG and EA have protective effects on cataract development via the inhibition of oxidative stress.
ABSTRACT
OBJECTIVES: To investigate the potential protective effects of sesamol in an experimental cataract model. MATERIALS AND METHODS: Twenty-one Spraque Dawley rat pups were randomly assigned into three groups, seven rats in each. All the rats except for those in the control group were injected subcutaneously with a single dose of sodium selenite on postpartum day 9. On days 10-14, rats in the sham group were intraperitoneally administered 50 mg/kg/day saline solution, while rats in the sesamol group were given 50 mg/kg/day sesamol by the same route. Following cataract grading, the lenses and capsules were extracted and the mean levels of reduced glutathione (GSH), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS) in lens supernatants were biochemically analyzed. RESULTS: The control group did not show any development of cataract. It was found that the mean cataract grade in the sesamol group was significantly lower than that of the sham group (p<0.05). The mean GSH level and TAS in the sesamol group were significantly higher than those of the sham group while the mean TOS and MDA level in the sesamol group were significantly lower than those of the sham group (p<0.05). CONCLUSION: Our study shows that sesamol reduces TOS and MDA level and increases TAS and GSH level in the lens and inhibits cataract formation.
ABSTRACT
The purpose of this study was to determine whether α-lipoic acid and fisetin have protective effects against cataract in a streptozotocin-induced experimental cataract model. Twenty-eight male BALB/C mice were made diabetic by the intraperitoneal administration of streptozotocin (200 mg/kg). Three weeks after induction of diabetes, mice were divided randomly into 4 groups in which each group contained 7 mice; fisetin-treated group (group 1), α-lipoic acid-treated group (group 2), fisetin placebo group (group 3), α-lipoic acid placebo group (group 4). Fisetin and α-lipoic acid were administered intraperitoneally weekly for 5 weeks. Cataract development was assessed at the end of 8 weeks by slit lamp examination, and cataract formation was graded using a scale. All groups developed at least grade 1 cataract formation. In the fisetin-treated group, the cataract stages were significantly lower than in the placebo group (p = 0.02). In the α-lipoic acid-treated group, the cataract stages were lower than in the placebo group but it did not reach to a significant value. Both fisetin and α-lipoic acid had a protective effect on cataract development in a streptozotocin-induced experimental cataract model. The protective effect of fisetin appears as though more effective than α-lipoic acid.
Subject(s)
Cataract/prevention & control , Diabetes Complications/prevention & control , Diabetes Mellitus, Experimental , Flavonoids/administration & dosage , Lens, Crystalline/pathology , Thioctic Acid/administration & dosage , Animals , Antioxidants/administration & dosage , Cataract/diagnosis , Cataract/etiology , Diabetes Complications/diagnosis , Diabetes Complications/etiology , Disease Progression , Drug Therapy, Combination , Flavonols , Lens, Crystalline/drug effects , Male , Mice , Mice, Inbred BALB C , Treatment OutcomeABSTRACT
OBJECTIVE:To compare the therapeutic effects of taurine and catalin eye drops.METHODS:All the mice were divided into the normal control group,the cataract control group,the taurine group and the catalin eye drops group.Except the normal control group,the rest ones were used to establish the galactose cataract models and then received eyedrop administration everyday.The contents of taurine,glycin and glutamic acid in crystalline lens were measured by high perfor?mance liquid chromatography.RESULTS:The contents of amino-acids were not found lower in the taurine group as com?pared with the catalin eye drops group.CONCLUSION:Taurine shows the preventive and therapeutic effects on the experi?mental cataract,which can be comparable to that of catalin eye drops or even more advantageous.
