Tratamiento de punta nasal mestiza en rinoplastía secundaria / Treatment of mestize tip nose in secundary rhinoplasty

ANTECEDENTES: la nariz mestiza constituye un reto en la adecuada trasformación estética y funcional de la rinoplastia, más aún cuando esta se realiza en una segunda intervención y los tejidos nasales ya se encuentran alterados, no pudiendo hacer una adecuada reconstrucción de las estructuras anatómicas sin el uso de injertos cartilaginosos de otras áreas del cuerpo. OBJETIVO: el propósito de este trabajo es de evaluar los resultados obtenidos de una reconstrucción de la punta nasal, realizada en pacientes que ya fueron sometidos a una rinoplastia sea esta secundaria o cuaternaria. METODOLOGIA: estudio retrospectivo, de casos de pacientes que se sometieron a una nueva intervención para mejorar aspectos estéticos y funcionales nasales, que ya fueron operados en más de 1 oportunidad. HALLAZGOS: se tiene un universo de 10 pacientes en los que se realizó una rinoplastia secundaria o cuaternaria donde se utilizó injerto costal para conformar el esqueleto cartilaginoso y el uso de un injerto de extensión septal, todo esto para mejorar la estética y funcionalidad nasal.

Blockchain technologies to mitigate COVID-19 challenges: A scoping review.

Background: As public health strategists and policymakers explore different approaches to lessen the devastating effects of novel coronavirus disease (COVID-19), blockchain technology has emerged as a resource that can be utilized in numerous ways. Many blockchain technologies have been proposed or implemented during the COVID-19 pandemic; however, to the best of our knowledge, no comprehensive reviews have been conducted to uncover and summarise the main feature of these technologies. Objective: This study aims to explore proposed or implemented blockchain technologies used to mitigate the COVID-19 challenges as reported in the literature. Methods: We conducted a scoping review in line with guidelines of PRISMA Extension for Scoping Reviews (PRISMA-ScR). To identify relevant studies, we searched 11 bibliographic databases (e.g., EMBASE and MEDLINE) and conducted backward and forward reference list checking of the included studies and relevant reviews. The study selection and data extraction were conducted by 2 reviewers independently. Data extracted from the included studies was narratively summarised and described. Results: 19 of 225 retrieved studies met eligibility criteria in this review. The included studies reported 10 used cases of blockchain to mitigate COVID-19 challenges; the most prominent use cases were contact tracing and immunity passports. While the blockchain technology was developed in 10 studies, its use was proposed in the remaining 9 studies. The public blockchain technology was the most commonly utilized type in the included studies. All together, 8 different consensus mechanisms were used in the included studies. Out of 10 studies that identified the used platform, 9 studies used Ethereum to run the blockchain. Solidity was the most prominent programming language used in developing blockchain technology in the included studies. The transaction cost was reported in only 4 of the included studies and varied between USD 10-10 and USD 5. The expected latency and expected scalability were not identified in the included studies. Conclusion: Blockchain technologies are expected to play an integral role in the fight against the COVID-19 pandemic. Many possible applications of blockchain were found in this review; however, most of them are not mature enough to reveal their expected impact in the fight against COVID-19. We encourage governments, health authorities, and policymakers to consider all blockchain applications suggested in the current review to combat COVID-19 challenges. There is a pressing need to empirically examine how effective blockchain technologies are in mitigating COVID-19 challenges. Further studies are required to assess the performance of blockchain technologies' fight against COVID-19 in terms of transaction cost, scalability, and/or latency when using different consensus algorithms, platforms, and access types.

Haploinsufficiency of Trp53 dramatically extends the lifespan of Sirt6-deficient mice.

Mammalian sirtuin 6 (Sirt6) is a conserved NAD+-dependent deacylase and mono-ADP ribosylase that is known to be involved in DNA damage repair, metabolic homeostasis, inflammation, tumorigenesis, and aging. Loss of Sirt6 in mice results in accelerated aging and premature death within a month. Here, we show that haploinsufficiency (i.e., heterozygous deletion) of Trp53 dramatically extends the lifespan of both female and male Sirt6-deficient mice. Haploinsufficiency of Trp53 in Sirt6-deficient mice rescues several age-related phenotypes of Sirt6-deficient mice, including reduced body size and weight, lordokyphosis, colitis, premature senescence, apoptosis, and bone marrow stem cell decline. Mechanistically, SIRT6 deacetylates p53 at lysine 381 to negatively regulate the stability and activity of p53. These findings establish that elevated p53 activity contributes significantly to accelerated aging in Sirt6-deficient mice. Our study demonstrates that p53 is a substrate of SIRT6, and highlights the importance of SIRT6-p53 axis in the regulation of aging.

Utility of Computed Tomography in Elbow Trauma Patients with Normal X-Ray Study and Positive Elbow Extension Test.

BACKGROUND: Elbow fractures are a common injury seen among emergency department trauma patients. Despite its high frequency, there is no standardized method of diagnosis using conventional x-ray imaging for trauma patients presenting with elbow pain and restricted elbow movement. OBJECTIVE: We aimed to assess trauma patients, using computed tomography (CT), who present with a positive elbow extension test and have no evident fracture on x-ray study. METHODS: Patients presented to our emergency department with elbow trauma and were evaluated between April 2010 and March 2011. A CT scan of the injured elbow was ordered for patients with pain on elbow extension (a positive elbow extension test) and no evidence of fracture on x-ray study. All CT and x-ray images were evaluated by a designated radiologist. RESULTS: One hundred and forty-eight patients presented to our emergency department with elbow trauma. Two patients were excluded from the study, one with former motion disability and another with an open fracture. In the remaining patients, there were 32 fractures in total. Forty-three of 114 patients without fracture signs had a positive elbow extension test and 4 of these patients refused CT imaging. Fractures were found in 5 (12.8%) of the 39 patients assessed with CT. CT imaging found that two of these patients had a radial head fracture, two others had an olecranon fracture, and one patient had a coronoid fracture. CONCLUSIONS: We recommend CT as an additional evaluation imaging study for trauma patients who have a positive elbow extension test and who present with no apparent fracture on x-ray imaging.

Espaço Univer-cidade e PELC: a experiência do grupo GEPLEC/UFPNA gestão do programa de esporte e lazer na cidade de curitiba / Space Universe-city and PELC: the experience of the group GEPLEC/UFPR managemente sport and leisure program in the city of curitiba

O presente texto visa relatar os caminhos didático-metodológicos norteadores da implantação do PELC/UFPR, no período de 2008 a 2009, desenvolvido pelo Grupo de Estudos e Pesquisas em Lazer, Espaço e Cidade da UFPR (GEPLEC), localizado no CEPELS/DEF/UFPR. Esta ação se consolidou de forma articulada entre ensino-pesquisaextensão, com intervenções práticas no âmbito do lazer para a comunidade e formação continuada para professores e educadores. Para tanto, os conteúdos como jogo, esporte, dança, lutas, teatro, música, capoeira, práticas corporais na natureza, entre outras ações, foram desenvolvidos, ampliados e transformados. Tal ação partiu do princípio que as experiências no tempo-espaço do lazer podem se constituir como uma prática sociocultural, pedagógica e política.

This paper aims at reporting the ways teaching and methodological guide the deployment of PELC/UFPR in the period 2008 to 2009, developed by the Group of Leisure Studies and Research, Space and the City UFPR (GEPLEC), located in CEPELS/DEF/UFPR. This action was consolidated in coordination between education-researchextension, with practical interventions in the context of recreation for the community and continuing education for teachers and educators. For both, the contents such as game, sports, dance, fights, drama, music, capoeira, bodily practices in nature, among other actions, have been developed, expanded and transformed. Such action was assumed that the experiments in space-time leisure can be constituted as a sociocultural practice, pedagogy and politics.

Improving the affinity of an anti-TNF? scFv by error prone PCR and staggered extention process(StEP) / 中国免疫学杂志

Objective:To improve the affinity of an anti TNF? scFv.Methods:Starting from an anti TNF? scFv gene a mutant phage antibody library was generated by error prone PCR.Affinity improved clones were selected and subjected to staggered extension process to shuffle the mutated sites.Mutants with further improved affinity were selected by bio panning.Affinity was judged by dot blot ELISA and thiocyanate elusion ELISA.Results:Seven affinity improved mutants were obtained from library constructed by error prone PCR.By StEP mediated shuffling of these 7 clones and via bio panning,mutants with further improved affinity were obtained.Conclusion:Combination of error prone PCR and StEP could be used to improve the affinity of antibodies. [

Construction of chimeric CblN/Grb2 and its expression in prokaryon / 解放军医学杂志

Objective To construct the pGEX-4T-2-CblN/Grb2, express and purify the corresponding chimeric protein; To investigate whether the chimeric CblN/Grb2 possesses ubiquitin ligase activity. Methods Total RNA of SK-BR-3 cells were isolated and reversely transcribed into cDNA, which were used as templates to amplify Grb2 SH2 by PCR. The gene fragment encoding the N-terminal part of Cbl protein (named as CblN) was amplified by PCR using pEFHACbl plasmid encoding human Cbl as templates. BamH I and EcoR V restriction enzyme digestion sites were introduced into both flanks of SH2 by overlapping extension PCR and the modified gene were cloned into pcDNA3.1(+). The pcDNA3.1(+)-CblN/Grb2 was obtained by replacing SH2 of CblN with Grb2SH2 and then used as templates to amplify CblN/Grb2 by PCR. The pGEX-4T-2-CblN/Grb2 was constructed by subcloning CblN/Grb2 into the prokaryotic expressing vector pGEX-4T-2. The GST-CblN/Grb2 fusion protein was expressed in E. coli of DH5? under IPTG induction and further purified with Glutathione Sepharose 4B. In vitro ubiquitination assay was performed to investigate whether the GST-CblN/Grb2 fusion protein is able to mediate auto-ubiquitinating reaction, namely whether it possesses ubiquitin ligase activity. Results The fusion expressing vector of pGEX-4T-2-CblN/Grb2 was successfully constructed; The GST-CblN/Grb2 fusion protein was correctly expressed and purified; In vitro ubiquitination assay indicated that GST-CblN/Grb2 fusion protein is able to mediate auto-ubiquitinating reaction and therefore possesses ubiquitin ligase activity. Conclusion Expression, purification of GST-CblN/Grb2 and identification of its′ activity have laid the foundation for further study of chimeric ubiquitin ligase′ effects on the growth of HER2 positive tumor cells.