Use of Thermography to Evaluate Alternative Crops for Off-Season in the Cerrado Region.

Future predictions due to climate change are of decreases in rainfall and longer drought periods. The search for new tolerant crops is an important strategy. The objective of this study was to evaluate the effect of water stress on the physiology and productivity of crops with potential for growing in the off-season period in the Cerrado, and evaluate correlations with the temperature of the canopy obtained by means of thermography. The experiment was conducted under field conditions, with experimental design in randomized blocks, in a split-plot scheme and four replications. The plots were: common bean (Phaseolus vulgaris); amaranth (Amaranthus cruentus); quinoa (Chenopodium quinoa); and buckwheat (Fagopyrum esculentum). The subplots were composed of four water regimes: maximum water regime (WR 535 mm), high-availability regime (WR 410 mm), off-season water regime (WR 304 mm) and severe water regime (WR 187 mm). Under WR 304 mm, the internal concentration of CO2 and photosynthesis were reduced by less than 10% in amaranth. Common bean and buckwheat reduced 85% in photosynthesis. The reduction in water availability increased the canopy temperature in the four crops and, in general, common bean was the most sensitive species, while quinoa had the lowest canopy temperatures. Furthermore, canopy temperature correlated negatively with grain yield, biomass yield and gas exchange across all plant species, thus thermal imaging of the canopy represents a promising tool for monitoring crop productivity for farmers, For the identification of crops with high water use management for research.

Effective removal of non-steroidal anti-inflammatory drug from wastewater by adsorption process using acid-treated Fagopyrum esculentum husk.

In this work, buckwheat husks (Fagopyrum esculentum) were modified by acid treatment and posteriorly employed to remove the ketoprofen in batch adsorption. The characterization results indicated that a more irregular surface with new empty spaces was generated after acid treatment. The adsorptive process was favored at acidic pH = 3. The dosage of 0.85 g L-1 was fixed for the kinetic and isothermal tests, obtaining good removal and capacity indications. The kinetic studies were better represented by pseudo-second-order, obtaining an experimental capacity of 74.3 mg g-1 for 200 mg L-1 of ketoprofen. An increase in temperature negatively affected the adsorption isotherm curves, resulting in a maximum capacity of 194.1 mg g-1. Thermodynamic results confirmed the exothermic nature of the process with physical forces acting. The adsorbent presented high efficiency in treating a synthetic effluent containing different drugs and salts, 71.2%. Therefore, adsorbent development from buckwheat husks treated with a strong acid is an excellent alternative, given the good removal results and the low cost for its preparation.

Influence of base saturation on buckwheat grain and flavonoid production / Influência da saturação por bases na produção de grãos e de flavonoides de trigo-mourisco

Fagopyrum esculentum is highly nutritious due to its proteins of high biological value and high fiber content. Its most relevant property is nonetheless its antioxidant activity, provided by the presence of flavonoids. It is an important pseudocereal in agriculture, animal production, and human food. The objective of study was to evaluate the grain yield and flavonoid production of Fagopyrum esculentum cultivars as a function of base saturation. The experiment was carried out in a greenhouse, in a completely randomized design with four replicates. Treatments were represented by cultivars IPR 91 and IPR 92 and five base saturation levels (9, 31, 53, 75, and 97 %), in a factorial arrangement (2 × 5). Agronomic attributes and total-flavonoid and rutin contents and production were evaluated. Subsequently, the data were subjected to statistical analysis. Base saturation does not affect flavonoid production. However, base saturation influences shoot dry matter yield (maximum at 62.0% base saturation) and grain yield (maximum at 9.00%) in IPR 92. The higher-yielding cultivar is IPR 92. This is the first scientific report of base saturation in the species.(AU)

Fagopyrum esculentum é muito nutritivo por conter proteínas com alto valor biológico e alto teor de fibras, a propriedade mais relevante dele é a atividade antioxidante, devido à presença de flavonoides. É um pseudocereal importante na agricultura, produção animal e alimentação humana. O objetivo desse trabalho foi avaliar a produção de grãos e de flavonoides em cultivares de Fagopyrum esculentum em função da saturação por bases. O experimento foi realizado em casa de vegetação em delineamento inteiramente casualizado, com quatro repetições. Os tratamentos foram as cultivares IPR 91 e IPR 92 e cinco saturações por bases (9, 31, 53, 75 e 97 %), em esquema fatorial (2 × 5). Foram avaliados atributos agronômicos e teor e produção de flavonoides totais e de rutina. Posteriormente os dados foram submetidos a análises estatísticas. Para a produção de flavonoides, a saturação por bases não influencia nos resultados. Porém, para a produção de matéria seca da parte aérea (produção máxima aos 62,0 % de saturação por bases) e para a produção de grãos da IPR 92 (produção máxima aos 9,00 %), a saturação por bases influencia. A cultivar de maior produção é a IPR 92. Esse é o primeiro relato científico de saturação por bases da espécie.(AU)

Sowing dates and node emission in buckwheat cultivars / Datas de semeadura e emissão de nós em cultivares de trigo mourisco

The aim of this study was to determine the rate of node appearance (RNA), the final number of nodes (FNN) and the period of node emission (PNE) in two buckwheat cultivars (Fagopyrum esculentum Moench) for different sowing dates, and to check variability in the RNA, FNN and PNE between the cultivars and sowing dates. The IPR91-Baili and IPR92-Altar cultivars were evaluated for 29 and 31 sowing dates from October to May in the 2017/2018 and 2018/2019 agricultural years respectively. In each experiment, a completely randomised design with five replications was used. The number of nodes (NN) on the main stem was counted twice a week in each plant (replication), from emission of the first node until the end of the cycle. The linear regression (y = a + bx) of the number of nodes (NN, y) was adjusted for each plant based on the number of days after emergence (DAE, x). The rate of node appearance (RNA), in days node-1, was determined from the inverse of the slope of the linear regression between NN and DAE (RNA = 1/b). The RNA was calculated for the period of node emission (PNE, in days), i.e. from emergence to emission of the last node. The final number of nodes (FNN) was counted at the end of node emission. Variability was found in the rate of node appearance (RNA), the final number of nodes (FNN) and the period of node emission (PNE) between the cultivars and sowing dates.(AU)

Os objetivos deste trabalho foram determinar a taxa de aparecimento de nós (TAN), o número final de nós (NFN) e o período de emissão de nós (PEN) em duas cultivares de trigo mourisco (Fagopyrum esculentum Moench) em datas de semeadura e verificar a variabilidade da TAN, do NFN e do PEN entre as cultivares e as datas de semeadura. As cultivares IPR91-Baili e IPR92-Altar foram avaliadas em 29 e 31 datas de semeadura, nos anos agrícolas 2017/2018 e 2018/2019, respectivamente, durante os meses de outubro a maio. Nos dois experimentos foi utilizado o delineamento inteiramente casualizado com cinco repetições. Em cada planta (repetição) foi contado o número de nós (NN) da haste principal, duas vezes por semana, a partir da emissão do primeiro nó até o final do ciclo. Para cada planta foi ajustada a regressão linear (y=a+bx) do número de nós (NN, y) em função do número de dias após a emergência (DAE, x). A taxa de aparecimento de nós (TAN), em dias nó-1, foi determinada pelo inverso do coeficiente angular da regressão linear entre o NN e o DAE (TAN=1/b). A TAN foi calculada para o período de emissão de nós (PEN, em dias), ou seja, entre a emergência até a emissão do último nó. O número final de nós (NFN) foi contabilizado na avaliação em que a planta cessou a emissão de nós. Há variabilidade da taxa de aparecimento de nós (TAN), do número final de nós (NFN) e do período de emissão de nós (PEN) entre as cultivares e as datas de semeadura. A taxa de aparecimento de nós oscila entre 2,20 e 8,23 dias nó-1, o número final de nós entre 2,20 e 17,20 nós e o período de emissão de nós entre 16,60 e 49,20 dias. As plantas das cultivares IPR91-Baili e IPR92-Altar, apresentam melhor desenvolvimento vegetativo (menor TAN, maior NFN e maior PEN) nas semeaduras nos meses de novembro, dezembro, janeiro e fevereiro em comparação com as semeaduras realizadas em outubro, março, abril e maio.(AU)

Estimation of buckwheat leaf area by leaf dimensions / Estimação de área foliar de trigo mourisco por dimensões foliares

The objective of this work was to model and identify the best models for estimating the leaf area, determined by digital photos, of buckwheat (Fagopyrum esculentum Moench) of the cultivars IPR91-Baili and IPR92-Altar, as a function of length (L), width (W) or length x width product (LW) of the leaf blade. Ten uniformity trials (blank experiments) were carried out, five with IPR91-Baili cultivar and five with IPR92-Altar cultivar. The trials were performed on five sowing dates. In each trial and cultivar, expanded leaves were collected at random from the lower, middle and upper segments of the plants, totaling 1,815 leaves. In these 1,815 leaves, L and W were measured and the LW of the leaf blade was calculated, which were used as independent variables in the model. The leaf area of each leaf was determined using the digital photo method (Y), which was used as a dependent variable of the model. For each sowing date, cultivar and thirds of the plant, 80% of the leaves (1,452 leaves) were randomly separated for the generation of the models and 20% of the leaves (363 leaves) for the validation of the models of leaf area estimation as a function of linear dimensions. For buckwheat, IPR91-Baili and IPR92-Altar cultivars, the quadratic model (Ŷ = 0.5217 + 0.6581LW + 0.0004LW2, R2 = 0.9590), power model (Ŷ = 0.6809LW1.0037, R2 = 0.9587), linear model (Ŷ = 0.0653 + 0.6892LW, R2 = 0.9587) and linear model without intercept (Ŷ = 0.6907LW, R2 = 0.9587) are indicated for the estimation of leaf area determined by digital photos (Y) based on the LW of the leaf blade (x), and, preferably, the linear model without intercept can be used, due to its greater simplicity.(AU)

O objetivo deste trabalho foi modelar e identificar os melhores modelos para a estimação da área foliar, determinada por fotos digitais, de trigo mourisco (Fagopyrum esculentum Moench) das cultivares IPR91-Baili e IPR92-Altar, em função do comprimento (C), da largura (L) ou do produto comprimento vezes largura (CL) do limbo foliar. Foram conduzidos dez ensaios de uniformidade (experimentos em branco), sendo cinco com a cultivar IPR91-Baili e cinco com a cultivar IPR92-Altar. Os ensaios foram realizados em cinco datas de semeadura. Em cada ensaio e cultivar foram coletadas, aleatoriamente, folhas expandidas dos terços inferior, médio e superior das plantas, totalizando 1.815 folhas. Nessas 1.815 folhas, foram mensurados o C e a L e calculado o CL do limbo foliar, os quais foram utilizados como variáveis independentes no modelo. Determinou-se a área de cada folha por meio do método de fotos digitais (Y) e a mesma foi utilizada como variável dependente do modelo. Para cada data de semeadura, cultivar e terços da planta foram separadas, aleatoriamente, 80% das folhas (1.452 folhas) para a geração de modelos e 20% das folhas (363 folhas) para a validação dos modelos de estimação da área foliar em função das dimensões lineares. Para o trigo mourisco, cultivares IPR91-Baili e IPR92-Altar, os modelos quadrático (Ŷ = 0,5217 + 0,6581CL + 0,0004CL2, R2 = 0,9590), potência (Ŷ = 0,6809CL1,0037, R2 = 0,9587), linear (Ŷ = 0,0653 + 0,6892CL, R2 = 0,9587) e linear sem intercepto (Ŷ = 0,6907CL, R2 = 0,9587), são indicados para a estimação da área foliar determinada por fotos digitais (Y) com base no CL do limbo foliar (x), podendo, preferencialmente, ser utilizado o modelo linear sem intercepto, devido a sua maior simplicidade.(AU)

Sowing dates and node emission in buckwheat cultivars / Datas de semeadura e emissão de nós em cultivares de trigo mourisco

The aim of this study was to determine the rate of node appearance (RNA), the final number of nodes (FNN) and the period of node emission (PNE) in two buckwheat cultivars (Fagopyrum esculentum Moench) for different sowing dates, and to check variability in the RNA, FNN and PNE between the cultivars and sowing dates. The IPR91-Baili and IPR92-Altar cultivars were evaluated for 29 and 31 sowing dates from October to May in the 2017/2018 and 2018/2019 agricultural years respectively. In each experiment, a completely randomised design with five replications was used. The number of nodes (NN) on the main stem was counted twice a week in each plant (replication), from emission of the first node until the end of the cycle. The linear regression (y = a + bx) of the number of nodes (NN, y) was adjusted for each plant based on the number of days after emergence (DAE, x). The rate of node appearance (RNA), in days node-1, was determined from the inverse of the slope of the linear regression between NN and DAE (RNA = 1/b). The RNA was calculated for the period of node emission (PNE, in days), i.e. from emergence to emission of the last node. The final number of nodes (FNN) was counted at the end of node emission. Variability was found in the rate of node appearance (RNA), the final number of nodes (FNN) and the period of node emission (PNE) between the cultivars and sowing dates.

Os objetivos deste trabalho foram determinar a taxa de aparecimento de nós (TAN), o número final de nós (NFN) e o período de emissão de nós (PEN) em duas cultivares de trigo mourisco (Fagopyrum esculentum Moench) em datas de semeadura e verificar a variabilidade da TAN, do NFN e do PEN entre as cultivares e as datas de semeadura. As cultivares IPR91-Baili e IPR92-Altar foram avaliadas em 29 e 31 datas de semeadura, nos anos agrícolas 2017/2018 e 2018/2019, respectivamente, durante os meses de outubro a maio. Nos dois experimentos foi utilizado o delineamento inteiramente casualizado com cinco repetições. Em cada planta (repetição) foi contado o número de nós (NN) da haste principal, duas vezes por semana, a partir da emissão do primeiro nó até o final do ciclo. Para cada planta foi ajustada a regressão linear (y=a+bx) do número de nós (NN, y) em função do número de dias após a emergência (DAE, x). A taxa de aparecimento de nós (TAN), em dias nó-1, foi determinada pelo inverso do coeficiente angular da regressão linear entre o NN e o DAE (TAN=1/b). A TAN foi calculada para o período de emissão de nós (PEN, em dias), ou seja, entre a emergência até a emissão do último nó. O número final de nós (NFN) foi contabilizado na avaliação em que a planta cessou a emissão de nós. Há variabilidade da taxa de aparecimento de nós (TAN), do número final de nós (NFN) e do período de emissão de nós (PEN) entre as cultivares e as datas de semeadura. A taxa de aparecimento de nós oscila entre 2,20 e 8,23 dias nó-1, o número final de nós entre 2,20 e 17,20 nós e o período de emissão de nós entre 16,60 e 49,20 dias. As plantas das cultivares IPR91-Baili e IPR92-Altar, apresentam melhor desenvolvimento vegetativo (menor TAN, maior NFN e maior PEN) nas semeaduras nos meses de novembro, dezembro, janeiro e fevereiro em comparação com as semeaduras realizadas em outubro, março, abril e maio.

Estimation of buckwheat leaf area by leaf dimensions / Estimação de área foliar de trigo mourisco por dimensões foliares

The objective of this work was to model and identify the best models for estimating the leaf area, determined by digital photos, of buckwheat (Fagopyrum esculentum Moench) of the cultivars IPR91-Baili and IPR92-Altar, as a function of length (L), width (W) or length x width product (LW) of the leaf blade. Ten uniformity trials (blank experiments) were carried out, five with IPR91-Baili cultivar and five with IPR92-Altar cultivar. The trials were performed on five sowing dates. In each trial and cultivar, expanded leaves were collected at random from the lower, middle and upper segments of the plants, totaling 1,815 leaves. In these 1,815 leaves, L and W were measured and the LW of the leaf blade was calculated, which were used as independent variables in the model. The leaf area of each leaf was determined using the digital photo method (Y), which was used as a dependent variable of the model. For each sowing date, cultivar and thirds of the plant, 80% of the leaves (1,452 leaves) were randomly separated for the generation of the models and 20% of the leaves (363 leaves) for the validation of the models of leaf area estimation as a function of linear dimensions. For buckwheat, IPR91-Baili and IPR92-Altar cultivars, the quadratic model (Ŷ = 0.5217 + 0.6581LW + 0.0004LW2, R2 = 0.9590), power model (Ŷ = 0.6809LW1.0037, R2 = 0.9587), linear model (Ŷ = 0.0653 + 0.6892LW, R2 = 0.9587) and linear model without intercept (Ŷ = 0.6907LW, R2 = 0.9587) are indicated for the estimation of leaf area determined by digital photos (Y) based on the LW of the leaf blade (x), and, preferably, the linear model without intercept can be used, due to its greater simplicity.

O objetivo deste trabalho foi modelar e identificar os melhores modelos para a estimação da área foliar, determinada por fotos digitais, de trigo mourisco (Fagopyrum esculentum Moench) das cultivares IPR91-Baili e IPR92-Altar, em função do comprimento (C), da largura (L) ou do produto comprimento vezes largura (CL) do limbo foliar. Foram conduzidos dez ensaios de uniformidade (experimentos em branco), sendo cinco com a cultivar IPR91-Baili e cinco com a cultivar IPR92-Altar. Os ensaios foram realizados em cinco datas de semeadura. Em cada ensaio e cultivar foram coletadas, aleatoriamente, folhas expandidas dos terços inferior, médio e superior das plantas, totalizando 1.815 folhas. Nessas 1.815 folhas, foram mensurados o C e a L e calculado o CL do limbo foliar, os quais foram utilizados como variáveis independentes no modelo. Determinou-se a área de cada folha por meio do método de fotos digitais (Y) e a mesma foi utilizada como variável dependente do modelo. Para cada data de semeadura, cultivar e terços da planta foram separadas, aleatoriamente, 80% das folhas (1.452 folhas) para a geração de modelos e 20% das folhas (363 folhas) para a validação dos modelos de estimação da área foliar em função das dimensões lineares. Para o trigo mourisco, cultivares IPR91-Baili e IPR92-Altar, os modelos quadrático (Ŷ = 0,5217 + 0,6581CL + 0,0004CL2, R2 = 0,9590), potência (Ŷ = 0,6809CL1,0037, R2 = 0,9587), linear (Ŷ = 0,0653 + 0,6892CL, R2 = 0,9587) e linear sem intercepto (Ŷ = 0,6907CL, R2 = 0,9587), são indicados para a estimação da área foliar determinada por fotos digitais (Y) com base no CL do limbo foliar (x), podendo, preferencialmente, ser utilizado o modelo linear sem intercepto, devido a sua maior simplicidade.

Fagopyrum esculentum at early stages copes with aluminum toxicity by increasing ABA levels and antioxidant system.

Aluminum toxicity (Al) is one of the main constraints for plant growth on acid soils. While most plants are sensitive to Al, some species have developed strategies to cope with this metal. Fagopyrum esculentum, Moench., var Mancan (Polygonaceae), despite being an aluminum-tolerant plant, shows root inhibition as a seedling during the first hours of exposure to Al, whereas at later times, it fully recovers. In this study, we assessed whether abscisic acid (ABA) levels and the antioxidant system might be involved in the early tolerance mechanisms of F. esculentum. The results showed that seedlings exposed to 50 µM Al for 3, 6, 12, 24, and 48 h showed decreases in the relative root growth (RRG), and there was an accumulation of Al in the root apex from 3 to 24 h. In addition, reactive oxygen species (ROS) levels increased, and were detected early after Al exposure; endogenous ABA levels increased and antioxidant enzyme activity increased, including catalase (CAT, EC1.11.1.6), glutathione reductase (GR, EC 1.6.4.2), ascorbate peroxidase (APX, EC 1.11.1.11), and superoxide dismutase (SOD, EC 1.15.1.1) activity. Seedlings treated with exogenous ABA also showed increased ROS levels and CAT and APX activity. The results suggest that after the first 12 h of Al treatment, root growth declines while ROS levels increase due to the entrance of Al into the root. However, the enzyme antioxidant system is promoted, which may impact the recovery of the root growth at later times and increasing levels of ABA might mediate this effect.

Characterization of the transcriptional profiles in common buckwheat (Fagopyrum esculentum) under PEG-mediated drought stress

BACKGROUND: Common buckwheat (Fagopyrum esculentum) is an important staple food crop in southwest China, where drought stress is one of the largest limiting factors that lead to decreased crop production. To reveal the molecular mechanism of common buckwheat in response to drought stress, we performed a comprehensive transcriptomics study to evaluate gene expression profiles of common buckwheat during PEG-mediated drought treatment. RESULTS: In total, 45 million clean reads were assembled into 53,404 unigenes with an average length of 749 bp and N50 length of 1296 bp. A total of 1329 differentially expressed genes (DEGs) were identified by comparing wellwatered and drought-treated plants, out of which 666 were upregulated and 663 were downregulated. Furthermore, we defined the functional characteristics of DEGs using GO and KEGG classifications. GO enrichment analysis showed that the DEGs were significantly overrepresented in four categories, namely, "oxidoreductase activity," "oxidation­reduction process," "xyloglucan:xyloglucosyl transferase activity," and "apoplast." Using KEGG pathway analysis, a large number of annotated genes were overrepresented in terms such as "plant hormone signal transduction," "phenylpropanoid biosynthesis," "photosynthesis," and "carbon metabolism." Conclusions: These results can be further exploited to investigate the molecular mechanism of common buckwheat in response to drought treatment and could supply with valuable molecular sources for abiotic-tolerant elite breeding programs in the future.