Optimal growth conditions to enhance Chlorella vulgaris biomass production in indoor phyto tank and quality assessment of feed and culture stock.

Commercial microalgae cultivation is a dynamic field with ongoing efforts to improve efficiency, reduce costs, and explore new applications. We conducted a study to examine how different light exposure periods affect Chlorella vulgaris's growth. We employed a Phyto tank batch system of approximately 3.5 L with LED light control, controlled airflow, and sterilized bags, maintained at 22.0 ± 2.0 °C indoors. Various methods, including spectrophotometry, and cell counter were employed to monitor Chlorella vulgaris growth under different light exposure cycles. Additionally, quality analysis as feed source was employed by proximate, amino acid, beta-glucan, and microbial content analysis. The results revealed significant variations in C. vulgaris biomass production based on light exposure duration. Notably, the 16:8-h light-dark photoperiod exhibited the highest biomass concentration, reaching 6.48 × 107 ± 0.50 cells/mL with an optical density (OD) of 1.165 absorbance at 682 nm. The 12:12-h light-dark photoperiod produced the second-highest biomass concentration, with 2.305 × 106 ± 0.60 cells/mL at an OD of 0.489. Proximate analysis of dry algae powder revealed low lipid content (0.48 %), high protein content (37.61 %), variable ash concentration (average 10.75 %), and a significant carbohydrate fraction (51.16 %) during extended daylight and shorter dark periods. Amino acid analysis identified nine essential amino acids, with glutamic acid being the most abundant (17.7 %) and methionine the least (0.4 %). Furthermore, quality analysis and microbiological assays demonstrated that the C. vulgaris biomass is well-suited for fish and livestock use as a feed source and possibility as human nutraceuticals. These findings can be considered more environmentally friendly and ethically sound due to the absence of genetic modification.

Effect of Replacing Corn Meal with Winged Bean Tuber (Psophocarpus tetragonolobus) Pellet on Gas Production, Ruminal Fermentation, and Degradability Using In Vitro Gas Technique.

The objective of this study is to evaluate the effects of replacing corn meal in ruminant diets with winged bean (Psophocarpus tetragonolobus) tubers (WBT) on ruminal fermentation, gas production parameters, and in vitro degradability. The study employed a completely random design (CRD) in its execution. The experimental design employed was a completely randomized design (CRD), featuring eleven levels of corn meal substitution with winged bean tubers pellet (WBTP) at 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. The levels were grouped into four categories of replacement: control (0% in the diet), low levels (10%, 20%, and 30% in the diet), medium levels (40%, 50%, 60%, and 70% in the diet), and high levels (80%, 90%, and 100% in the diet). The experimental results indicated that substituting corn meal with WBTP at moderate and high levels in the diet could improve the performance of the fermentation process by increasing the gas production rate constant from the insoluble fraction (p < 0.01). The IVDMD exhibited a higher degree of in vitro degradation after 12 h (h), with the mean value being higher in the high group compared to the medium until the high group (p < 0.05). At the 4 h mark, the groups that substituted corn meal with WBTP exhibited a decrease in pH value (p < 0.05) in comparison to the control group. The substitution of corn meal with WBTP resulted in the lowest protozoal count after 8 h in the median group (p < 0.05). A significant difference in the effect of WBTP on total volatile fatty acid (TVFA) concentration was observed at 8 h after incubation (p < 0.05). The medium and high levels of WBTP replacement resulted in the lowest TVFA concentration at 8 h (p < 0.05). The mean proportion of acetic acid (C2) linearly declined and was lowest when a high level of WBTP replaced cornmeal (p < 0.05). The concentration of propionic acid (C3) at 8 h after incubation and average values were linearly significantly different when various levels of WBTP were utilized. Replacing corn meal with WBTP at a high level showed the highest concentration of C3. Moreover, substituting medium and high concentrations of WBTP for corn meal resulted in a significant reduction in both the C2:C3 ratio at 8 h and the mean value (p < 0.05). In conclusion, WBTP exhibits a nutritional composition that is advantageous and may be an energetic substitute for corn meal.

Proteolytic enzyme-treated seaweed co-product (Ulva laetevirens) inclusion in corn-soybean and European broiler diets to improve digestibility, health, and performance.

The impact of addition of an Ulva laetevirens (previously Ulva rigida) co-product treated with a broad-spectrum endo-protease when added to a standard corn-soy (S) based diet and a diet based on European protein sources (EU) on performance, in vivo digestibility and multiple gastrointestinal characteristics was investigated in broilers. In total, 624 Ross 308 one-day-old male broilers were fed one of 6 experimental diets (8 replicates) consisting of a basal diet (S or EU), or a basal diet including the U. laetevirens co-product (U) treated without (U-) or with (U+) a proteolytic enzyme. Starter diets contained 0 (wk 1) and 2.5 (wk 2), and the grower diets (wk 3 and 4) 5% seaweed co-product. In the last 2 wk, birds fed the S vs. EU grower diets showed a higher BW, BWG, and FI, as well as a lower FCR (-0.05 g/g) in wk 3 (P < 0.05). Heavier gizzards (+13%; P < 0.001) and heavier gizzard contents (+92%; P < 0.001) were observed in birds fed the EU vs. S diets, as well as longer villi (+8%; P = 0.010). U diets had a higher water holding capacity than the basal diets (+19%). In wk 4, U inclusion resulted in increased FCR (+0.06 g/g; P < 0.001), water intake (+7%; P < 0.001), and duodenal cross section (+5%; P = 0.033). Enzyme treatment did not affect digestibility of any nutrients, except for ash which was increased in birds fed U+ vs. U- diets (+60%; P < 0.001). U in S diets led to higher, and U in EU diets led to lower apparent pre-cecal digestibility of all nutrients (P < 0.001 for all nutrients). Although for both diet types performance was decreased, dietary U. laetevirens inclusion had different effects when added to a standard corn-soy diet and a diet based on European protein sources. No obvious health effects were observed, leading to the conclusion of the absence of performance of health promoting bioactive components in the U. laetevirens co-product, or of diminishing of these effects due to the proteolytic enzyme treatment.

A proteolytic enzyme treatment to improve Ulva laetevirens and Solieria chordalis seaweed co-product digestibility, performance, and health in broilers.

To explore the potential use of seaweed co-products for broiler diets, this study investigates whether an enzyme treatment of seaweed co-products improves performance, in vivo digestibility and health in broilers. In total, 360 Ross 308 male broilers were fed one of 5 experimental diets: a basal diet, or a basal diet including the U. laetevirens or S. chordalis co-product, with or without proteolytic enzyme treatment of the seaweed, using 6 replicate pens of 12 birds each. The starter (d 0-13) and grower (d 14-21) diet contained 5 and 10% (w/w) seaweed product, respectively. A general linear model with contrast statements was used after model assumptions and goodness of fit were evaluated through normal distribution of residuals. Inclusion of seaweed in the broiler diets increased body weight gain (+14%; P = 0.002), and feed intake (+12%; P = 0.001) in the third week of the experiment. Birds fed the U. laetevirens compared to the S. chordalis diets had a higher body weight gain (+11%; P = 0.007), and a lower feed conversion ratio (FCR; -7%; P < 0.001). Seaweed inclusion reduced apparent pre-cecal digestibility of all nutrients (P < 0.05). Birds fed U. laetevirens vs. S. chordalis diets had a 10% reduced villus length (P < 0.001). Enzymatic treatment reduced the digestibility of most nutrients, and increased crypt depth in birds fed the U. laetevirens diets, whereas the opposite was observed for the birds fed the S. chordalis diets (Seaweed × Enzyme P = 0.035). Untreated vs. treated seaweed in the diets led to lower (-60%) plasma Interleukin-13 levels (P = 0.035). In conclusion, the proteolytic enzyme treatment of the seaweed co-products did not improve performance nor health-related parameters, and reduced digestibility of the diets. Dietary inclusion of U. laetevirens co-products did improve performance based on growth and FCR, whereas inclusion of S. chordalis did not. Inclusion of U. laetevirens in broiler diets slightly reduced duodenal villus length and crypt depth. The inflammation response was strongly reduced, specifically in birds fed the untreated U. laetevirens diet, making the U. laetevirens co-product of interest for future research.

Meat Quality and Sensory Traits of Finisher Broiler Chickens Fed with Black Soldier Fly (Hermetia Illucens L.) Larvae Fat as Alternative Fat Source.

At present, there is limited knowledge about the possible utilization of the fat fraction derived from Hermetia illucens (HI) larvae processing. The objective of the present study was to evaluate the replacement of soybean oil with HI larvae fat in broiler finisher diet, on the quality and sensory traits of their meat. At 21 days of age, 120 male broiler chickens were randomly allocated to three experimental groups (5 replicates and 8 birds/pen): a basal control diet (C), and two groups in which either 50% or 100% of the soybean oil was replaced with HI larvae fat (the HI50 and HI100 group, respectively). At day 48, 15 birds (3 birds/pen) per group were slaughtered, and breasts and legs were excised and used for meat quality evaluations. Breast and leg physical meat quality, nutritional composition and sensory profile remained substantially unaffected by the dietary treatments. In contrast, the dietary incorporation of HI larvae fat modified the fatty acid (FA) profile of both the breast and leg meat cuts: the proportion of saturated fatty acids increased (p < 0.0001) to the detriment of the polyunsaturated (PUFA) fraction (p < 0.0001). Moreover, the meat n-6/n-3 ratio increased in the HI50 and HI100 groups compared to the C group. HI larvae fat dietary inclusion decreased the monounsaturated fatty acids in the breast (p = 0.0012) but not in the leg meat. Further research should focus on the improvement of the FA profile of the larvae through substrate modulation, or by combining HI larvae with a PUFA-rich feedstuff in feed formulations.

Fontes florais usadas por abelhas (Hymenoptera, Apoidea) em área de cerrado no município de Cassilândia, Mato Grosso do Sul, Brasil / Floral sources used by bees (Hymenoptera, Apoidea) in a savannah area of Cassilândia county, Mato Grosso do Sul, Brazil

Conduziu-se este trabalho, com o objetivo de inventariar as plantas visitadas por abelhas em uma área de cerrado no município de Cassilândia/MS (19°06'48"S; 51°44'03"W), classificando-as para a elaboração de um catálogo de pasto apícola. Os dados foram obtidos quinzenalmente, de março/2003 a fevereiro/2004, em uma trilha com 3000 metros de extensão. A flora apícola foi representada por 49 espécies pertencentes a 41 gêneros e 26 famílias. A família Malpighiaceae apresentou maior número de espécies visitadas (12,2 por cento) e a família Sapindaceae o maior número de abelhas coletadas (18 por cento). Com relação ao nicho trófico ocupado pelas abelhas, apenas Apis mellifera e Trigona spinipes apresentaram atividade de forrageamento em um grande número de plantas, sendo 36,7 por cento do total de espécies identificadas visitados pelas duas espécies. Os diferentes períodos de florescimento das espécies vegetais existentes no cerrado garantem oferta de recurso alimentar durante todo o ano.

The aim of this study is listing the plants visited by bees in a savannah area of Cassilândia/MS, Brazil (19°06'48"S; 51°44'03"W) to develop bee plant catalogue. The data were obtained twice a month from March/2003 to February/2004, along a 3 km track. The flora was represented by 49 species, 41 genera and 26 families. The Malpighiaceae family presented the highest number of visited species (12,2 percent) and Sapindaceae family the highest number of bees collected (18 percent). Regarding to the trofic niche used by bees, only Apis mellifera and Trigona spinipes presented collection activity in a great number of plants so that, 36,7 percent of the total of identified plant species was visited by the two species. The different blossom periods of plant species in savannah guarantee the occurrence of feed source for bees during all year long.