Mercury concentrations and differences in isotopic niches of fish from upstream and downstream of an Amazon reservoir dam.

Reservoir construction promotes many environmental impacts, including the enhancement of mercury concentrations in fish. The processes that can influence mercury concentrations in fish in Amazonian reservoirs are still little explored in depth, especially when we consider the possible particularities of the ecosystems in question. This study aims to investigate how mercury concentrations in fish could be influenced by the Tucuruí dam, considering possible changes in their feeding and trophic position according to the dam position (up or downstream). Fish were sampled upstream and downstream of the Tucuruí reservoir, and total mercury (THg) and stable isotopes of carbon and nitrogen (δ15N and δ13C) were measured in muscles. We observed three different Hg bioaccumulation patterns influenced by the dam. These differences occurred due to species trophic niche changes corroborated by the isotope analysis. Higher THg concentrations downstream compared to those upstream ones were only observed for Geophagus proximus. On the contrary, Plagioscion squamosissimus, from downstream, presented lower concentrations than upstream ones. The isotopic niche of these two species presented different changes according to the sampled site. THg biomagnification was higher upstream compared to downstream, considering that the regression slope was approximately two times higher upstream versus downstream. THg concentrations in fish were explained by the differences in their feeding habits according to their location in relation to the dam. The difference in THg biomagnification was able to reflect differences in structure of the food web chain in ecosystems under the dam's influence.

Evidence of microplastics in the polychaete worm (capitellids-Capitella capitata) (Fabricicus, 1780) along Thoothukudi region.

Research on the occurrence of microplastics in invertebrates of the Thoothukudi region is limited. Capitellids are non-selective suspension feeders and are usually used as bioindicator of water pollution. Hence, an investigation was carried out to identify the microplastic occurrence in the capitellids (Capitella capitata) (Fabricius, 1780) collected from the Vellapatti and Spic Nagar sites of the Thoothukudi region. Result from this investigation showed the occurrence of 0.21 ± 0.17 items/indiv and a mean abundance of 13.33% in Thoothukudi coast. The mean microplastic abundance in the capitellids was significantly higher in the Spic Nagar (0.26 ± 0.19 MPs/indiv), probably due to the dumping of plastic waste, fishing and recreational activities. However, no significant difference was observed between seasons. Only fragments (Vellapatti 66.66% and Spic Nagar 33.33%) and fibre-shaped microplastics (Vellapatti 50% and Spic Nagar 50%) were identified. The size and colour of the microplastics dominant in both sites were 1-2 mm (Vellapatti 77.77% and Spic Nagar 75%) and blue (Vellapatti 88.88% and Spic Nagar 87.5%), respectively. The results of Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) analysis revealed the presence of polyethylene (PE) and polypropylene (PP) polymers in the capitellids. PE polymer is one of the most common sources of microplastics contamination globally and it is also frequently found in the coastal waters of Thoothukudi. This accounted for the high occurrence of PE polymers in the capitellids with the occurrence rate of 77% in Vellapatti and 58.52% in Spic Nagar. The present study provides baseline data on the occurrence, characterization (shapes, sizes and colours) and qualitative analysis of the microplastics in the capitellids, and their presence was influenced by their non-selective feeding habits. Further, future studies have to be conducted to identify the levels of microplastics in different polychaetes and other invertebrates to better understand the effects of microplastic pollution in invertebrate communities.

Inocellia (Amurinocellia) calida (Raphidioptera, Inocelliidae) was first observed as a predator of Monochamussaltuarius (Coleoptera, Cerambycidae) in China, the vector of Bursaphelenchusxylophilus (Aphelenchida, Aphelenchoididae).

Monochamussaltuarius Gebler (Coleoptera, Cerambycidae) serves as the primary carrier of Bursaphelenchusxylophilus (Steiner & Buhrer) (Aphelenchida, Aphelenchoididae) in the middle-temperate zone of China. Pine wilt disease caused by B.xylophilus leads to serious losses to pine forestry around the world. It is necessary to study the biological control of M.saltuarius to effectively prevent the further spread of B.xylophilus. To explore the insect resources that act as natural enemies of M.saltuarius, investigations were conducted on natural enemy insects by splitting Pinuskoraiensis Siebold & Zucc (Pinales, Pinaceae) damaged by M.saltuarius and dissecting their trunks in Yingpan Village, Fushun County, Fushun City, Liaoning Province, China, in 2023. A larva of Inocellia (Amurinocellia) calida (H. Aspöck & U. Aspöck) (Raphidioptera, Inocelliidae) was discovered in the trunk of an infested P.koraiensis. Additionally, the feeding habits of I.calida were preliminarily examined under indoor conditions and a description of its morphological characteristics was provided. When placed in an indoor environment, the I.calida larva began pupating after a period of 21 days, during which time it consumed and attacked a total of 23 M.saltuarius larvae. Ultimately, after a pupal period of ten days, the I.calida larva emerged successfully as an adult. This discovery marks the first recorded presence of I.calida in Liaoning Province and the first documentation of I.calida in China, serving as a natural predatory enemy of M.saltuarius.

Tongue microarchitecture and functional characterization of the lingual papillae in the desert hedgehog (Paraechinus aethiopicus).

The present work attempted to provide a comprehensive description of the morphoanatomical, histological, and ultrastructural characteristics of the tongue in the desert hedgehog (Paraechinus aethiopicus), and to correlate lingual modifications to the feeding lifestyle. Five adult male hedgehogs were utilized in our investigation. The macroscopic observations revealed elongated, with a moderately pointed apex, tongue and the tongue dorsum lacks both lingual prominence and median sulcus. The main subdivisions of the tongue are radix linguae (root), corpus linguae (body), and apex linguae (apex). The tongue dorsum carries two types of mechanical (conical and filiform) and gustatory (fungiform and circumvallate) papillae. The lingual apex is characterized by the existence of a unique encapsulated muscular structure. Additionally, the lingual glands were interposed between the muscular strands and no lingual glands were detected on the lingual apex. The dorsal surface of the lingual apex exhibited the highest level of keratinization as revealed by histochemical staining while the root showed moderate staining. The topography of the tongue was investigated by scanning electron microscopy (SEM). The obtained results are important to provide basic knowledge that can contribute to better understanding of the nourishment, feeding habits and behavior in this species. Furthermore, the addition of the newly investigated species may help us to determine the evolutionary relationships among species.

A comparison of digestive strategies for fishes with different feeding habits: Digestive enzyme activities, intestinal morphology, and gut microbiota.

Fish feeding habit determines the digestive tract structure and intestinal microflora. However, the relationship between feeding habit, digestive intestinal morphology, and microbial diversity of omnivorous, herbivorous, plankton feeder, and carnivorous fish from the same environment has not been compared. This study compared the digestive enzyme activities, intestinal morphology, and intestinal microflora of omnivorous (Carassius auratus), herbivorous (Ctenopharyngodon idellus), carnivorous (Siniperca chuatsi), and plankton feeder (Schizothorax grahami) fishes and predicted the potential functions of specific microflora on different nutrients. Twelve intestine samples were collected from each of the four fishes from Dianchi Lake. The composition and diversity of microbial communities were determined by using high-throughput sequencing of 16S rDNA. The results showed that the carnivorous fish (S. chuatsi) had higher trypsin and pancrelipase activities in the hepatopancreas and enteropeptidase in the intestine, but lower amylase activities in the intestine. The carnivorous fish intestine had more microvilli branches and complex structures than other fish species in the order carnivorous > herbivorous > plankton feeder > omnivorous. The intestinal microflora diversity was higher in the omnivorous fish and followed the order omnivorous > herbivorous > plankton feeder > carnivorous. Acinetobacter species and Bacteroides species were the most dominant flora in the carnivorous and herbivorous fishes, respectively. Acinetobacter species and Pseudomonas species might help the host to digest protein, while Bacteroidetes species may help the host to digest cellulose. Taken together, feeding habit determines the digestive enzyme activities, intestinal tissue morphology, and differential colonization of fish intestinal flora. The knowledge obtained is useful in feed formulation and feeding practices for the studied fish species.

Biological features, distribution, and conservation of the near-threatened Gangetic leaf fish Nandus nandus (Hamilton, 1822): A review.

Nandus nandus (Hamilton, 1822) is a small indigenous species (SIS) and commercially important food fish found in Bangladesh, India, Nepal, Pakistan, Myanmar, and Thailand. Concerns have arisen due to the gradual decline in its abundance, reflected by poor catches and limited availability of this species in local markets. Habitat loss and overexploitation pose significant threats to this species, leading to its classification as near threatened by the IUCN Bangladesh. Despite the absence of dedicated conservation efforts for N. nandus in Indian subcontinent, this study aims to investigate the species' biology, ecology, distribution within the country and globally, as well as its threats, conservation strategies, and research needs. Additionally, a proposed conservation framework for this near-threatened species is presented, which can be adapted for implementation in other nations facing similar challenges.

Application of high-throughput sequencing to analyze the diet of black croaker (Atrobucca nibe) in southern coastal waters of Zhejiang.

This study investigated the feeding habits of black croaker (Atrobucca nibe) to fully understand its role in the food web in southern coastal waters of Zhejiang, China. In total, 225 black croakers were collected in a bottom trawl survey, of which 195 were used for the stomach contents analysis and 30 for high-throughput sequencing (HTS). Both approaches showed that the main prey was shrimp, with Alpheus japonicus being the most predominant. In addition, black croaker also fed on other benthic species, suggesting that it was a demersal predator. Feeding intensity also varied with black croaker body length, as did prey preference, from small-sized species to larger species with increases in fish size; in addition, prey species were more diverse in the large-bodied prey group. Canonical correspondence analysis indicated that the feeding habit of black croaker was most significantly correlated with temperature and dissolved oxygen. HTS was shown to be a feasible high precision semi-quantitative analytical approach, although it must be combined with morphological methods to obtain more complete feeding data; thus, it could provide an effective scientific basis for feeding ecology studies of marine organisms.

Morphometry and food preference in relation to sex and hematological values of Eurasian collared dove (Streptotella decaocto).

Objective: The study investigated the gut content and recorded morphometric and hematological parameters in the Eurasian collared dove (Streptopelia decaocto). Materials and Methods: 24 samples of healthy birds (12 from each sex) were collected from different wetlands in Punjab, Pakistan, from December 2022 until February 2023. Birds were captured live for blood samples, morphometric, and gut analyses. Results: The current study revealed that mensural measurements showed no significant differences in all parameters except tail length, which was significantly longer in males (14.59 ± 0.30) compared to females (12.88 ± 0.43). Hematological parameters were hemoglobin, 23.95 gm/dl; red blood cells, 3.97 × 106/µl; white blood cells, 429.9.67 × 103/µl; hematocrit, 72.14%; mean corpuscular volume, 183.24 FL; mean corpuscular hemoglobin, 61.70 pg; mean corpuscular hemoglobin concentration, 32.37 pg; platelets, 7.01/µl; and red cell distribution width, 110.86/µl. The percentages of neutrophils, lymphocytes, monocytes, and eosinophils were 71.33%, 23.03%, 3.30%, and 1.43%, respectively. The gut content of the Eurasian collared dove mainly consisted of rice, wheat, corn, and millet seeds. Some stony materials were also present. Conclusion: Our study concluded that male and female Eurasian collared doves are alike in biometrics (except tail length) and hematological profiles. Gut content and weight were also similar. Males were slightly larger than females. The gut content showed that the Eurasian collared dove mainly feeds on rice, wheat, corn, and millet seeds. In this study, hematological parameters were also studied.

Comparative study of the digestion and metabolism related genes' expression changes during the postnatal food change in different dietary mammals.

The changes in the expression of genes related to digestion and metabolism may be various in different dietary mammals from juvenile to adult, especially, the giant panda (Ailuropoda melanoleuca) and red panda (Ailurus fulgens), which were once carnivores but have shifted to being specialized bamboo eaters, are unique features of their changes are more unclear. To elucidate the changing patterns of gene expression related to digestion and metabolism from juvenile to adult in different dietary mammals, we performed transcriptome analysis of the liver or pancreas in giant and red pandas, herbivorous rabbits (Oryctolagus cuniculus) and macaques (Macaca mulatta), carnivorous ferrets (Mustela putorius furo), and omnivorous mice (Mus musculus) from juvenile to adult. During the transition from juvenile to adulthood, giant and red pandas, as well as rabbits and macaques, show significant upregulation of key genes for carbohydrate metabolism, such as starch hydrolysis and sucrose metabolism, and unsaturated fatty acid metabolism, such as linoleic acid, while there is no significant difference in the expression of key genes for fatty acid ß-oxidation. A large number of amino acid metabolism related genes were upregulated in adult rabbits and macaques compared to juveniles. While adult giant and red pandas mainly showed upregulation of key genes for arginine synthesis and downregulation of key genes for arginine and lysine degradation. In adult stages, mouse had significantly higher expression patterns in key genes for starch hydrolysis and sucrose metabolism, as well as lipid and protein metabolism. In contrast to general expectations, genes related to lipid, amino acid and protein metabolism were significantly higher expressed in adult group of ferrets, which may be related to their high metabolic levels. Our study elucidates the pattern of changes in the expression of genes related to digestion and metabolism from juvenile to adult in different dietary mammals, with giant and red pandas showing adaptations associated with specific nutritional limitations of bamboo.

Dataset of microplastics in the mangrove brachyuran crabs at Setiu Wetlands, Peninsular Malaysia.

The present dataset provides information on the abundance of microplastics (MPs) in relation to different feeding habits of the four mangrove brachyuran crab species namely, Parasesarma eumolpe, Austruca annulipes, Metaplax elegans and Scylla olivacea at Setiu Wetlands in Peninsular Malaysia. Three sites namely, Pulau Layat (upstream, close to the core mangrove zone), Kampung Pengkalan Gelap (midstream, close to the settlements), and Pulau Sutung (downstream, close to the shifted river mouth) were chosen for the sample collection (through hand catch method and traps) in both the dry (Feb-Mar 2021) and the wet (Dec 2021 - Jan 2022) seasons. The cardiac stomach of each crab was dissected, digested in potassium hydroxide and then filtered through a 1.6 µm pore size glass fibre filter using the vacuum pump. The abundance, type and colour of MPs per crab individual were determined under a stereomicroscope (Carl Zeiss Stemi 508, China) attached to the digital camera (Axiocam 208 colour). The general abundance of MPs was found in the order of carnivorous S. olivacea > microphytobenthos feeder A. annulipes > herbivorous P. eumolpe > detritivorous M. elegans. The data also reveal morphometric measurements such as body weight, gut weight, carapace width and carapace length of the crab specimens. The information given in this article is useful for study replications and scientific comparisons, especially with brachyuran crabs and other organisms with similar feeding guilds, in the mangroves of Malaysia and elsewhere.

Effects and regulatory pathway of proopinmelanocortin on feeding habit domestication in mandarin fish.

Proopiomelanocortin (POMC) is a hormone precursor, and has been reported to participate in domestication. However, its effects on feeding habit domestication in fish are poorly understood. Mandarin fish (Siniperca chuatsi) feeds solely on live prey fish since first-feeding. In the present study, the high expression of pomc in mandarin fish, both the pomc siRNA and MC4R inhibitor treatments increased the success rate of domestication from live prey fish to dead prey fish and food intake of dead prey fish, suggesting the role of pomc on the special feeding habit of live prey fish in mandarin fish. In addition, one c-fos binding site was identified in the region that from -1053 bp to -931 bp upstream of the transcription start site of pomc, and this region exhibited positive promoter activity. The mandarin fish brain cells treated with c-fos siRNA displayed suppressed pomc mRNA expression, indicating that c-fos positively regulated pomc expression. Furthermore, the mRNA expression of c-fos was higher in the mandarin fish which were more difficult to domesticate. The results of ChIP assay and inhibitor treatment confirmed that the activation of c-fos gene by histone H3K4me3 was catalyzed by Setd1b in mandarin fish. Three open peaks were found at the upstream regulatory region of setd1b by ATAC-seq, and the mRNA expression of setd1b was higher in the mandarin fish which were more difficult to domesticate. These results indicated that Setd1b could methylate histone H3K4 to activate the c-fos transcription, maintaining the high expression of pomc, which might contribute to the special feeding habit of mandarin fish.

Residue character of polycyclic aromatic hydrocarbons in river aquatic organisms coupled with geographic distribution, feeding behavior, and human edible risk.

Polycyclic aromatic hydrocarbons (PAHs) residues in fish, shrimp and shellfish have attracted attention because they are major species in the aquatic food chain and an important food source for humans. These organisms have various feeding habits and different living environments, and through the food chain, they can directly or indirectly connect particulate organic matter and human consumption. However, little attention has been paid to the bioaccumulation of PAHs in aquatic organism groups representing varied conditions and feeding habits in the food chain. In this study, 17 species of aquatic organisms, comprising fish, shrimp, and shellfish, were captured from 15 locations distributed within the river network of the Pearl River Delta. The concentration of 16 PAHs was measured in the aquatic organisms. The sum of the 16 measured PAHs ranged from 57.39 to 696.07 ng/g, dry weight, while phenanthrene had the highest individual content. The linear mixed effect model was applied to estimate the random effects of PAH accumulation in aquatic organisms. The result showed that the contributed proportion of variance to feeding habits (58.1 %) was higher than that of geographic distribution (11.8 %). In addition, one-way analysis of variance (ANOVA) demonstrated that the concentrations of PAHs depended on the water layer inhabited by the organism and its species status. Specifically, shellfish and carnivorous bottom-dwelling fish showed significantly higher levels than other aquatic organisms.

Assessment of Stock Status, Metal Contents with Human Health Risk of Gudusia chapra from Oxbow lake, Bangladesh.

The current study is focused on a comprehensive stock assessment of Gudusia chapra to assess the stock status along with feeding habits, essential minerals content and risk to human health from exposure to heavy metals. A total of 723 specimens from the Bukvora Baor, Bangladesh were used to estimate the total body length (TL) and body weight (W) which ranged from 5.5 to 14.5 cm and 1.62-26.45 g, respectively. The estimated asymptotic length (L ∞) of this species (15.38) was in comparison with average length at 10 cm from 723 specimens along with the rate of 0.70 yr-1 for approaching to asymptotic length. The obtained growth performance index (φ) 2.2 indicates that this species is not economically feasible to bring under aquaculture. At an average annual water surface temperature of 28 °C, natural mortality, 1.71yr-1 indicating good ecological suitability of Bukvora oxbow lake (Baor). The estimated current exploitation ratio (0.24) reflected under-exploitation status with total instantaneous mortality (2.25 years-1) and fishing mortality (0.55 years-1). The recruitment pattern of this species was observed across the year, with main peak occurring during the period of April to May. The estimated total steady state biomass (3.91 metric ton) and MSY (4.40 metric ton) based on length-structured virtual population analysis (VPA) length-structured virtual population analysis (VPA) through FiSAT II schedule indicating the sustainable production of this species. The obtained values of proximate compositions (% of protein, fat, Moisture, ash) showed no significant variations during different seasons throughout the year. The monthly measurements of GaSI showed significant alterations (p < 0.05). The value of essential minerals (Na and Ca) was recorded 9.18 mg and 245.19 mg per 100 g of fish flesh, respectively. Target hazard quotient and Cancer risk for all detected heavy metals were far below the recommended levels by United States Environmental Protection Agency. Therefore, the target fish species from oxbow lake is safe and have no human health risk. Therefore, the outcomes of this research would be very worthwhile in carrying out specific management for G. chapra in Baor ecosystems.

Feeding habit and pollution indicating food items of Mystus gulio (Hamilton, 1822) in the inward waters of Mumbai coast.

The study considered factors related to feeding habits such as food, frequency of occurrence (F), feeding index (IA) and vacancy index (VI). The aim was to determine the relationship between the feeding habits of a non-selective, euryphagous, voracious and pollution-tolerant fish species in a contaminated habitat. The seasonal food and feeding pattern of Mystus gulio, long whiskered catfish, from Ulhas river estuary and Thane Creek analysed from stomach content depicted the pollution status of the ambient environment based on the feeding pattern using box-whiskered plot, MDS and cluster analysis. Coconut husk fibres, eggshells, chicken feathers, pieces of brick, sand particles, plastic fibres, etc., represented the non-food component of anthropogenic origin. The presence of non-food items indicates the severely degraded conditions in the study areas, especially in Thane Creek. Although a native fish species tolerant of pollution, the survival of M. gulio in Thane Creek is threatened.

Study of feeding biology and diet-associated microplastic contamination in selected creek fishes of northeastern Arabian Sea: A multi-species approach.

This study investigated the diet composition and microplastic contamination in six fish species collected from the creek area of northeastern Arabian Sea. The results show that the diet of the fish is mainly composed of shrimps, algae, fish, and zooplankton, with microplastics constituting up to 4.83 % (Index of Preponderance) of their diet. The average abundance of microplastics ranges from 5.82 to 7.69 items per fish, and their ingestion is influenced by seasonal variation, gut fullness, and trophic level. Microplastic contamination has no significant effect on the condition factor and hepatosomatic index of the fish species. However, polymer hazard index indicates that microplastic pollution in fish is associated with a low to high risk factor, which might cause potential harm to aquatic lives and higher vertebrates via food chain. Therefore, this study highlights the need for immediate attention and effective regulations to reduce microplastic pollution to protect marine life.

Ingestion of microplastics in commercially important species along Thoothukudi coast, south east India.

Microplastics pollute the marine environment and pose a greater risk to marine organisms. The microplastics were observed in the guts of the 12 species, which varied from 0.00 to 1.80 ± 1.19 particles /individual. Most of the microplastics were fibre shaped, 0.5-1 mm sized, blue-coloured, and polyethylene polymers. The abundance of the microplastics was higher for benthic species (0.66 ± 0.13 particles/ individual) than the pelagic species (0.53 ± 0.11 particles/individual), with no significant difference (p > 0.05). According to their feeding habits and trophic level, significantly the microplastics were abundant in the herbivores (1.23 ± 0.61 particles/individual) and quaternary consumers (0.76 ± 0.16 particles/individual), respectively. The present study suggests that microplastic ingestion in commercially important species was influenced by their feeding habits irrespective of their habitat and length and weight. In addition to this, biomagnification of the microplastics (Trophic Magnification Factor, TMF = 1.02) was also observed in the commercially important species with increasing trophic level. This further indicates that the trophic level can serve as the pathway for the transfer of microplastics from lower trophic level organisms to higher trophic level organisms. The present study concludes that the occurrence of biomagnification of microplastics and the pollutants absorbed by them might harm the commercially important species from the Thoothukudi region.

Distribution Pattern of Mangrove Fish Communities in China.

Mangroves are among the most productive marine and coastal ecosystems and play an important role in maintaining the stability and diversity of fish communities. To explore the structure of mangrove fish communities in China, we compiled previous studies, monographs, and two databases on 54 mangrove areas published in the past 30 years. Mangrove fish communities in China comprised Osteichthys (597 species) and Chondrichthyes (14 species), representing 611 species in 344 genera, 117 families, and 28 orders. Perciformes were the predominant taxon, with 350 species in 52 families, accounting for 57% of the total species richness. Reef fish accounted for 29.62%. With regard to feeding groups, there were 328 carnivorous species (53.68%), 214 omnivorous species (35.02%), 41 herbivorous species (6.71%), and 28 detritivores species (4.58%). Classified by body size, 57.61% were small-sized, 24.22% medium-sized, and 18.17% were large-sized fishes. A total of 5.23% (32 species) of these mangrove fish are currently on IUCN red lists, i.e., 2 species are critically endangered, 4 are endangered, 12 are vulnerable, and 14 are near threatened. Cluster analyses shows that Chinese mangroves fish were divided into two categories, i.e., coastal mangrove and island mangrove type. This is closely related to the distribution of reef fish. Moreover, the number of fish species showed a strong positive correlation with mangrove area, but not with latitude. The main reasons may be the subtropical and tropical geographic locations, as well as the characteristics of the South China Sea and the Taiwan Warm Current. The size and integrity of mangrove area are crucial to the local ecosystems; thus, protecting and restoring mangroves is of great significance to large-scale ecosystem-stability and local biodiversity.

Nutritional and metabolic process of the dung beetle Phelotrupes auratus depends on the plant ingredients that the herbivores eat.

BACKGROUND: The dung beetle Phelotrupes auratus is a holometabolous insect belonging to the order Coleoptera, and it is widely distributed in Japan. The P. auratus habitat depends on herbivores. P. auratus eats the dung of the herbivores and carries it underground for its young. In this process, herbivore droppings disappear from the ground, not only keeping the ground hygienic but also maintaining good soil conditions for plant growth. In this way, a rich ecosystem is maintained. In recent years, the population of P. auratus has decreased, and the main cause has been the decrease in grazing land. It seems that Japanese dung beetles are mainly dependent on herbivores for nutrient sources. However, the physiological relationship between herbivores and P. auratus has not been well investigated. Here, we investigated the nutritional metabolism system of P. auratus by performing whole gene expression analysis of individuals collected from two areas where the ecosystem is occupied by different herbivores. RESULTS: We obtained 54,635 transcripts from P. auratus from Nara Park and Cape Toi and identified 2,592 differentially expressed genes in the fat bodies of the Nara Park and Cape Toi groups. We annotated P. auratus transcripts using Homo sapiens and Drosophila melanogaster genes as references; 50.5% of P. auratus transcripts were assigned to H. sapiens genes, and 54.0% of P. auratus transcripts were assigned to D. melanogaster genes. To perform gene set enrichment analysis, we chose H. sapiens genes for P. auratus transcript annotation. Principal component analysis and gene set enrichment analysis revealed that the nutritional metabolism of P. auratus from Cape Toi might differ from that of P. auratus from Nara Park. CONCLUSION: We analyzed the nutritional metabolism system of P. auratus from Cape Toi and Nara Park and found that the characteristics of the nutritional metabolism process might depend on the plants consumed by the herbivores. Our findings will contribute to elucidating the relationships among habitat plants, herbivores, and dung decomposers and may aid in the maintenance of sustainable land health cycles.

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Decapterus maruadsi is an important economic pelagic fish in the southern coastal area of Zhejiang. According to the bottom trawl surveys conducted in May, August, November 2020 and January 2021, the feeding habits of D. maruadsi in the southern coastal area of Zhejiang were examined by both stable isotope (carbon, δ13C, and nitrogen, δ15N) and stomach content analyses. Results showed that the δ13C value of D. maruadsi ranged from -17.76‰ to -15.25‰, with a mean of (-16.55±0.60)‰, while the δ15N value ranged from 9.06‰ and 13.03‰, with a mean of (11.76±0.88)‰. There was a significant negative correlation between the δ13C values and fork length, and a positive relationship between the δ15N with fork length. Results from stomach content analysis showed that the main prey groups of D. maruadsi were fish, shrimp, crabs, Cephalopod, Polychaete, and small crustaceans. As for the stable isotope analysis, the nutritional contribution rate of shrimp was the highest (40%-84%) among all prey groups, followed by Polychaete, small crustaceans, crabs, Cephalopods and fish. Significant ontogenetic dietary changes were found for D. maruadsi. As the fork length of D. maruadsi increased, it tended to eat prey from higher trophic levels.