How do invasive predators and their native prey respond to prescribed fire?

Fire shapes animal communities by altering resource availability and species interactions, including between predators and prey. In Australia, there is particular concern that two highly damaging invasive predators, the feral cat (Felis catus) and European red fox (Vulpes vulpes), increase their activity in recently burnt areas and exert greater predation pressure on the native prey due to their increased exposure. We tested how prescribed fire occurrence and extent, along with fire history, vegetation, topography, and distance to anthropogenic features (towns and farms), affected the activity (detection frequency) of cats, foxes, and the native mammal community in south-eastern Australia. We used camera traps to quantify mammal activity before and after a prescribed burn and statistically tested how the fire interacted with these habitat variables to affect mammal activity. We found little evidence that the prescribed fire influenced the activity of cats and foxes and no evidence of an effect on kangaroo or small mammal (<800 g) activity. Medium-sized mammals (800-2000 g) were negatively associated with prescribed fire extent, suggesting that prescribed fire has a negative impact on these species in the short term. The lack of a clear activity increase from cats and foxes is likely a positive outcome from a fire management perspective. However, we highlight that their response is likely dependent upon factors like fire size, severity, and prey availability. Future experiments should incorporate GPS-trackers to record fine-scale movements of cats and foxes in temperate ecosystems immediately before and after prescribed fire to best inform management within protected areas.

Small islands as potential model ecosystems for parasitology: climatic influence on parasites of feral cats.

The influence of climate on parasite distribution has been demonstrated in different regions worldwide. Despite its small size, Gran Canaria (Canary Islands, Spain) constitutes a 'biodiversity laboratory' due to the huge climatic differences between municipalities. Feral cats may represent a threat to biodiversity due to their predatory behaviour. In addition, they may be a source of pathogens zoonotic to humans. To study the climatic/seasonal influence and prevalence of feral cat parasites throughout the island, a total of 290 stool samples from 29 feral cat colonies were analysed following standard concentration protocols (sodium chloride, formol-ether and zinc sulphate). In total, 13 feline parasitic taxa were found, with the most common species being Ancylostoma spp., which, together with Toxocara spp., Toxoplasma gondii and Giardia spp., are considered a concern for human health. Nematodes were the most common parasites in all areas. Nematodes and protozoans were significantly more prevalent in temperate mild (75.0% and 30.0%) than in dry desert areas (29.3% and 18.7%). In contrast, cestodes were significantly more prevalent in dry desert than in temperate mild areas (26.0% and 13.3%). Only protozoans exhibited statistically significant seasonal patterns, mostly in the wet season. Data reported in this study endorse the usage of small and diverse islands such as Gran Canaria to study the climatic influence on parasitic communities in wild/feral animals. Cat colonies require better management to reduce their threat to endemic wildlife, domestic animals and public health, being invasive species that harbour zoonotic parasites.

Review and synthesis of the global literature on domestic cat impacts on wildlife.

A vast global literature documents that free-roaming domestic cats (Felis catus) have substantial negative effects on wildlife, including through predation, fear, disease and competition-related impacts that have contributed to numerous wildlife extinctions and population declines worldwide. However, no study has synthesized this literature on cat impacts on wildlife to evaluate its overarching biases and major gaps. To direct future research and conservation related to cat impacts on wildlife, we conducted a global literature review that entailed evaluation and synthesis of patterns and gaps in the literature related to the geographic context, methods and types of impacts studied. Our systematic literature search compiled 2245 publications. We extracted information from 332 of these meeting inclusion criteria designed to ensure the relevance of studies analysed. This synthesis of research on cat impacts on wildlife highlights a focus on oceanic islands, Australia, Europe, and North America, and on rural areas, predation, impacts of unowned cats, and impacts at population and species levels. Key research advances needed to better understand and manage cat impacts include more studies in underrepresented, highly biodiverse regions (Africa, Asia, and South America), on cat impacts other than predation, and on methods designed to reduce impacts on wildlife. The identified areas of needed research into cat impacts on wildlife will be critical to further clarifying the role of cats in global wildlife declines and to implementing science-driven policy and management that benefit conservation efforts.

The Implications of Policies on the Welfare of Free-Roaming Cats in New Zealand.

A lack of national legislation for cat management in New Zealand poses challenges for ensuring that practices are consistently humane and effective. In this paper, we review the current cat management policies in New Zealand and the implications they have on the welfare of free-roaming cats (from here on, referred to as 'cats'). Our review demonstrates that there are multiple policy mechanisms used to manage cats in New Zealand for a variety of reasons, including animal welfare, pest management, and nuisance, and that these different policies have both positive and negative implications for cat welfare. We provide context pertaining to New Zealanders' acceptance of current or future laws and regulations and compare the New Zealand policy landscape with other countries, with a particular emphasis on Australia, to identify potential directions and outcomes of increased regulation. We discuss the future of the regulatory environment in New Zealand, including the need to better understand the impact of policies on cats, people, and other animals in urban, rural, and wild spaces. We further discuss the need to better understand the cat-human relationship for future policy decisions and offer a solution based on national cat legislation.

Guidance for management of free-roaming community cats: a bioeconomic analysis.

OBJECTIVES: This study used computer simulation modeling to estimate and compare costs of different free-roaming cat (FRC) management options (lethal and non-lethal removal, trap-neuter-return, combinations of these options and no action) and their ability to reduce FRC population abundance in open demographic settings. The findings provide a resource for selecting management approaches that are well matched for specific communities, goals and timelines, and they represent use of best available science to address FRC issues. METHODS: Multiple FRC management approaches were simulated at varying intensities using a stochastic individual-based model in the software package Vortex. Itemized costs were obtained from published literature and expert feedback. Metrics generated to evaluate and compare management scenarios included final population size, total cost and a cost efficiency index, which was the ratio between total cost and population size reduction. RESULTS: Simulations suggested that cost-effective reduction of FRC numbers required sufficient management intensity, regardless of management approach, and greatly improved when cat abandonment was minimized. Removal yielded the fastest initial reduction in cat abundance, but trap-neuter-return was a viable and potentially more cost-effective approach if performed at higher intensities over a sufficient duration. Of five management scenarios that reduced the final population size by approximately 45%, the three scenarios that relied exclusively on removal were considerably more expensive than the two scenarios that relied exclusively or primarily on sterilization. CONCLUSIONS AND RELEVANCE: FRCs present a challenge in many municipalities, and stakeholders representing different perspectives may promote varying and sometimes incompatible population management policies and strategies. Although scientific research is often used to identify FRC impacts, its use to identify viable, cost-effective management solutions has been inadequate. The data provided by simulating different interventions, combined with community-specific goals, priorities and ethics, provide a framework for better-informed FRC policy and management outcomes.

"State of the Mewnion": Practices of Feral Cat Care and Advocacy Organizations in the United States.

Over the last several decades, feral cats have moved from the fringes to the mainstream in animal welfare and sheltering. Although many best practice guidelines have been published by national non-profits and veterinary bodies, little is known about how groups "in the trenches" actually operate. Our study sought to address that gap through an online survey of feral cat care and advocacy organizations based in the United States. Advertised as "The State of the Mewnion," its topics included a range of issues spanning non-profit administration, public health, caretaking and trapping, adoptions of friendly kittens and cats, veterinary medical procedures and policies, data collection and program efficacy metrics, research engagement and interest, and relationships with wildlife advocates and animal control agencies. Respondents from 567 organizations participated, making this the largest and most comprehensive study on this topic to date. Respondents came primarily from grassroots organizations. A majority reported no paid employees (74.6%), served 499 or fewer feral cats per year (75.0%), engaged between 1 and 9 active volunteers (54.9%), and did not operate a brick and mortar facility (63.7%). Some of our findings demonstrate a shared community of practice, including the common use of a minimum weight of 2.0 pounds for spay/neuter eligibility, left side ear tip removals to indicate sterilization, recovery holding times after surgery commonly reported as 1 night for male cats and 1 or 2 nights for females, requiring or recommending to adopters of socialized kittens/cats that they be kept indoor-only, and less than a quarter still engaging in routine testing of cats for FIV and FeLV. Our survey also reveals areas for improvement, such as most organizations lacking a declared goal with a measurable value and a time frame, only sometimes scanning cats for microchips, and about a third not using a standardized injection site for vaccines. This study paints the clearest picture yet available of what constitutes the standard practices of organizations serving feral and community cats in the United States.

Two Methods of Monitoring Cats at a Landscape-Scale.

Feral cats are difficult to manage and harder to monitor. We analysed the cost and the efficacy of monitoring the pre- and post-bait abundance of feral cats via camera-traps or track counts using four years of data from the Matuwa Indigenous Protected Area. Additionally, we report on the recovery of the feral cat population and the efficacy of subsequent Eradicat® aerial baiting programs following 12 months of intensive feral cat control in 2019. Significantly fewer cats were captured in 2020 (n = 8) compared to 2019 (n = 126). Pre-baiting surveys for 2020 and 2021 suggested that the population of feral cats on Matuwa was very low, at 5.5 and 4.4 cats/100 km, respectively, which is well below our target threshold of 10 cats/100 km. Post-baiting surveys then recorded 3.6 and 3.0 cats/100 km, respectively, which still equates to a 35% and 32% reduction in cat activity. Track counts recorded significantly more feral cats than camera traps and were cheaper to implement. We recommend that at least two methods of monitoring cats be implemented to prevent erroneous conclusions.

SARS-CoV-2 infection in domestic and feral cats: current evidence and implications.

Current evidence indicates that cats play a limited role in COVID-19 epidemiology, and pets are probably dead-end hosts of SARS-CoV-2 and pose negligible risks of transmission to humans. Still, one health concept is to be adopted widely as a component of mitigation strategies to tackle the ongoing pandemic. Therefore, in terms of the magnitude of infection and potential to transmit SARS-CoV-2 to humans, our surveillance efforts should mainly focus on mustelids (especially minks, ferrets, and others) for early detection and control of infection. This will ensure that SARS-CoV-2 will not get established in the wild animal population of these susceptible species. We agree with Dr. Passarella Teixeira on the possibility of domestic and feral cats acting as an urban reservoir, subsequently transmitting the virus to human beings. However, it is less likely that such a phenomenon will be reported even if it has occurred due to the efficient and extensive human-to-human transmission of SARS-CoV-2.

Managing ecosystems in a sea of uncertainty: invasive species management and assisted colonizations.

Managing ecosystems in the face of complex species interactions, and the associated uncertainty, presents a considerable ecological challenge. Altering those interactions via actions such as invasive species management or conservation translocations can result in unintended consequences, supporting the need to be able to make more informed decisions in the face of this uncertainty. We demonstrate the utility of ecosystem models to reduce uncertainty and inform future ecosystem management. We use Phillip Island, Australia, as a case study to investigate the impacts of two invasive species management options and consider whether a critically endangered mammal is likely to establish a population in the presence of invasive species. Qualitative models are used to determine the effects of apex predator removal (feral cats) and invasive prey removal (rabbits, rats, and mice). We extend this approach using Ensemble Ecosystem Models to consider how suppression, rather than eradication influences the species community; and consider whether an introduction of the critically endangered eastern barred bandicoot is likely to be successful in the presence of invasive species. Our analysis revealed the potential for unintended outcomes associated with feral cat control operations, with rats and rabbits expected to increase in abundance. A strategy based on managing prey species appeared to have the most ecosystem-wide benefits, with rodent control showing more favorable responses than a rabbit control strategy. Eastern barred bandicoots were predicted to persist under all feral cat control levels (including no control). Managing ecosystems is a complex and imprecise process. However, qualitative modeling and ensemble ecosystem modeling address uncertainty and are capable of improving and optimizing management practices. Our analysis shows that the best conservation outcomes may not always be associated with the top-down control of apex predators, and land managers should think more broadly in relation to managing bottom-up processes as well. Challenges faced in continuing to conserve biodiversity mean new, bolder, conservation actions are needed. We suggest that endangered species are capable of surviving in the presence of feral cats, potentially opening the door for more conservation translocations.

Prevalence of Feline Immunodeficiency Virus and Toxoplasma gondii in Feral Cats on St. Kitts, West Indies.

Toxoplasma gondii (T. gondii) is a cosmopolitan protozoan parasite that infects all warm-blooded species including humans. The definitive hosts of T. gondii are felid vertebrates including the domestic cat. Domestic cats shed oocysts for approximately two weeks in their feces after the primary infection. It has been shown that feline immunodeficiency virus (FIV) positive cats have a higher prevalence of and a higher titer of antibodies to T. gondii than those of FIV-negative cats. The main purposes of this study were to determine FIV prevalence and to investigate the oocysts shedding in FIV-positive and FIV-negative feral cats on St. Kitts. Fecal samples were collected from feral cats while their FIV statues were determined using a commercial SNAP kit. Total fecal DNA of each cat was tested for the presence of T. gondii DNA using a polymerase chain reaction (PCR) consistently detecting one genome equivalent. A FIV-positive status was detected in 18 of 105 (17.1%, 95% confidence interval (CI): 9.9%-24.3%) feral cats sampled. Furthermore, males were three times more likely to be FIV positive than females (p = 0.017) with an odds ratio of 3.93 (95% CI: 1.20-12.89). Adults were found to have at least twice the prevalence of FIV compared to cats younger than one year of age (p = 0.056) with an odds ratio of 3.07 (95% CI: 0.94-10.00). Toxoplasma gondii DNA was not detected in the feces of any of the 18 FIV-positive (95% CI: 0%-0.18%) and 87 FIV-negative cats (95% CI: 0%-0.04%). A follow-up study with a much bigger sample size is needed to prove or disprove the hypothesis that FIV-positive cats have a higher prevalence of shedding T. gondii oocysts than FIV-negative cats.

A triple threat: high population density, high foraging intensity and flexible habitat preferences explain high impact of feral cats on prey.

Alien mammalian carnivores have contributed disproportionately to global loss of biodiversity. In Australia, predation by the feral cat and red fox is one of the most significant causes of the decline of native vertebrates. To discover why cats have greater impacts on prey than native predators, we compared the ecology of the feral cat to a marsupial counterpart, the spotted-tailed quoll. Individual prey are 20-200 times more likely to encounter feral cats, because of the combined effects of cats' higher population densities, greater intensity of home-range use and broader habitat preferences. These characteristics also mean that the costs to the prey of adopting anti-predator behaviours against feral cats are likely to be much higher than adopting such behaviours in response to spotted-tailed quolls, due to the reliability and ubiquity of feral cat cues. These results help explain the devastating impacts of cats on wildlife in Australia and other parts of the world.

Corrigendum: "State of the Mewnion": Practices of Feral Cat Care and Advocacy Organizations in the United States.

[This corrects the article DOI: 10.3389/fvets.2021.791134.].

Measuring, evaluating and improving the effectiveness of invasive predator control programs: Feral cat baiting as a case study.

Reducing the impacts of invasive predators is a key objective for conservation managers, livestock producers and human health agencies globally. The efficacy of invasive predator control programs, however, is highly variable. To improve control efficacy, managers require a fundamental understanding of the factors that contribute to the success or failure of a control program. Using a predator baiting program as a case study, we measured the efficacy of baiting as a control tool to significantly reduce feral cat (Felis catus) populations. We used camera traps and cat-borne GPS collars to monitor changes in feral cat populations at a baited site and an unbaited site, using a Before-After, Control-Impact (BACI) design. We also identified five key elements required for a successful baiting program (bait encounter rate, availability, attractiveness, palatability and lethality) and simultaneously measured these to identify areas for potential improvement. Baiting was ineffective at reducing feral cat populations; collared cat mortality was only 11% (1/9), with camera traps revealing negligible reductions in the number of cat detection events (9%), naïve occupancy (15%), and no significant change in the relative abundance of feral cats (F1,54 = 0.8641, P = 0.357). Several factors contributed to the poor control efficacy. Bait encounter rates were low, with cats active along tracks (where baits were laid) < 4% of the time. Cats encountered only 14% (7/50) of monitored baits, but none were eaten. Initially, baits appeared attractive to cats; however meat ants and desiccation rapidly decreased bait palatability. Bait availability to cats declined rapidly, with 36% of monitored baits (18/50) removed by non-target species within the first 48 h. The mortality of one collared cat and chemical assays confirmed that, on average, each bait contained sufficient 1080 to kill a large (>5 kg) feral cat. Our findings suggest that altering bait deployment patterns, increasing bait densities and improving bait palatability could potentially improve the efficacy of baiting programs to reduce feral cat populations. Our study provides a framework to measure and evaluate the key elements that contribute to efficacy of pest control programs, and to identify opportunities for improving outcomes of future control programs.

Development of the Mata Hari Judas Queen (Felis catus).

Cats (Felis catus) are significant predators of mammals, birds, frogs and reptiles and are implicated in mammal species extinctions in Australia. Current controls fail to eradicate entire populations allowing survivors to re-establish. The use of the Mata Hari Judas (MHJ) technique, i.e., inducing prolonged oestrus using hormone implants, can enhance the eradication of remnant animals and would greatly improve conservation efforts. The hypotheses tested were that hormone implants could induce prolonged oestrus in queens (adult female cats), and that prolonging oestrus would result in sustained attractiveness to toms (adult male cats). Queens (n = 14) were randomly allocated to five treatments including a control and four treatments using hormone implants. Queens were observed daily; alone and during indirect contact with a tom for 30 consecutive days. There were significant increases (p < 0.001) in oestrus duration (19 to 27 days) for entire and ovariohysterectomised queens given Compudose100™ implants (1/8 or 1/4 implant). This study shows that it is possible to induce and prolong oestrus in queens using Compudose100™ implants where these queens are attractive to toms. The MHJ queen is a new tool with the potential to enhance the detection and thus the control of feral cats in remnant populations.

Space use and habitat selection of an invasive mesopredator and sympatric, native apex predator.

BACKGROUND: Where mesopredators co-exist with dominant apex predators, an understanding of the factors that influence their habitat and space use can provide insights that help guide wildlife conservation and pest management actions. A predator's habitat use is defined by its home range, which is influenced by its selection or avoidance of habitat features and intra- and inter-specific interactions within the landscape. These are driven by both innate and learned behaviour, operating at different spatial scales. We examined the seasonal home ranges and habitat selection of actively-managed populations of a native apex predator (dingo Canis dingo) and invasive mesopredator (feral cat Felis catus) in semi-arid Western Australia to better understanding their sympatric landscape use, potential interactions, and to help guide their management. METHODS: We used kernel density estimates to characterise the seasonal space use of dingoes and feral cats, investigate inter- and intra-species variation in their home range extent and composition, and examine second-order habitat selection for each predator. Further, we used discrete choice modelling and step selection functions to examine the difference in third-order habitat selection across several habitat features. RESULTS: The seasonal home ranges of dingoes were on average 19.5 times larger than feral cats. Feral cat seasonal home ranges typically included a larger proportion of grasslands than expected relative to availability in the study site, indicating second-order habitat selection for grasslands. In their fine-scale movements (third-order habitat selection), both predators selected for roads, hydrological features (seasonal intermittent streams, seasonal lakes and wetlands), and high vegetation cover. Dingoes also selected strongly for open woodlands, whereas feral cats used open woodlands and grasslands in proportion to availability. MANAGEMENT RECOMMENDATIONS: Based on these results, and in order to avoid unintended negative ecological consequences (e.g. mesopredator release) that may stem from non-selective predator management, we recommend that feral cat control focuses on techniques such as trapping and shooting that are specific to feral cats in areas where they overlap with apex predators (dingoes), and more general techniques such as poison baiting where they are segregated.

Cat ownership and Proximity to Significant Ecological Areas Influence Attitudes Towards Cat Impacts and Management Practices.

Cat ownership is increasing globally, representing a growing threat to urban wildlife. Although some cities have policies and strategies for managing owned cats, the companionship value placed on cats makes such management contentious. Prioritizing cat management in urban residential zones adjacent to large significant ecological areas (SEAs; areas designated on the basis of representativeness, threat status or rarity, diversity, connectedness, or uniqueness) could maximize return on management effort. Residents in these areas may place a relatively higher value on nature than residents in suburbs with minimal or no SEAs, and therefore may be comparatively more likely to perceive cats' wildlife impacts as important. We used a quantitative survey to compare SEA and non-SEA suburbs' residents' attitudes towards cat impacts and management in Tamaki Makaurau-Auckland, Aotearoa-New Zealand. Participants were asked to rate the importance of different feral and owned cat impacts, the importance of feral-cat control in different locations, and various ownership behaviors in terms of acceptability and best practice. SEA suburb residents placed more importance on wildlife predation impacts of feral cats and were more likely to regard 24-h cat confinement as best practice than non-SEA suburb residents. However, we also found that cat ownership and youth were negatively associated with perception of cat impacts, and owners were less likely to accept belled collars and cat confinement than nonowners. Therefore, although targeting SEA adjacent areas for cat management holds promise for reducing resident contention, proximity to such areas is a relatively minor influence for cat owners.

Managing feral cats through an adaptive framework in an arid landscape.

Adaptive management is the systematic acquisition and application of reliable information to improve natural resource management over time. We have employed an adaptive management framework in the control and monitoring of feral cats (Felis catus) on the Matuwa Indigenous Protected Area over the past 16 years. We used 120 Reconyx PC900 camera-traps and a rapid survey technique called the cat track activity index (TAI) to determine if aerial baiting with Eradicat® was more efficient and/or cost-effective than track baiting plus leg-hold trapping. We found that aerial baiting at $0.54 per percent decrease in cat detections is more cost-effective than track-baiting alone at $0.56 per percent decrease in cat detections. Track baiting plus leg-hold trapping, however, is more cost-effective than aerial baiting alone at reducing the number of feral cats detections at $0.39 per percent decrease in cat detections. Aerial baiting plus trapping was the most effective method of suppressing feral cats in an arid landscape with 97.7% reduction in cat detections. Trapping reduced the proportion of the population made up of adult cats from 51.5% to 38.7%, which may influence the efficacy of Eradicat®. Additionally, we found that cats were twice as likely to be detected on spinifex sandplain habitats than stony or hardpan habitats. We make several recommendations for refining feral cat management programs and future research.

Application of a high-quality, high-volume trap-neuter-return model of community cats in Seoul, Korea.

BACKGROUND: This study was performed to determine the characteristics of community cats that were admitted to trap-neuter-return(TNR) programs and the feasibility of the high-quality, high-volume spay/neuter (HQHVSN) model in Seoul, Korea. METHODS: TNR programs were performed eight times from 2017 to 2018, and a total of 375 community cats from the pilot areas were admitted. The pilot areas were selected regions wherein caregivers voluntarily participated in our TNR program. Each cat was anesthetized, assessed for health status, sterilized, vaccinated against feline viral rhinotracheitis, calicivirus and panleukopenia (FVR-CP), and rabies, and treated with insecticide after surgery. The time from anesthesia to recovery was evaluated to verify the efficiency of surgical time of the program. The TNR program at a local animal hospital and the program in this study were compared to assess the veterinary treatment administered and the cost for each cat. RESULTS: A total of 375 cats were underwent TNR in this study, including 192 (51.2%) intact females, 180 (48%) intact males, and three (0.8%) sterilized cats. Following surgery, 372 cats (99.2%) were returned to their original locations. Three cats (0.8%) died postoperatively. On average, 21.9% of the cats were pregnant during the TNRs, and the highest percentage of cats (63.9%) were pregnant in March. All cats presented to the TNR program were considered healthy enough to be part of the program after examination. For neutering one animal at a time, similar to conventional TNR ($140), it took 53 ± 16 mins for females and 30 ± 9 mins for males from anesthesia to recovery. In contrast, the standardized procedure can neuter multiple cats simultaneously, similar to the conveyor system, at an estimated rate of 6.8 mins per cat ($45 per female cat, $30 per male cat). CONCLUSIONS: The TNR strategy in Seoul should be implemented by establishing dedicated clinics to concentrate on HQHVSN surgeries of cats. Through this pilot study, we were able to demonstrate that it is possible to effectively apply HQHVSN clinics in Korea. Future studies that perform intensive sterilization in targeted areas are necessary to confirm the efficacy of the TNR strategy.

A native apex predator limits an invasive mesopredator and protects native prey: Tasmanian devils protecting bandicoots from cats.

Apex predators can limit the abundance and behaviour of mesopredators, thereby reducing predation on smaller species. We know less about whether native apex predators are effective in suppressing invasive mesopredators, a major global driver of vertebrate extinctions. We use the severe disease-induced decline of an apex predator, the Tasmanian devil, as a natural experiment to test whether devils limit abundance of invasive feral cats and in turn protect smaller native prey. Cat abundance was c. 58% higher where devils had declined, which in turn negatively affected a smaller native prey species. Devils had a stronger limiting effect on cats than on a native mesopredator, suggesting apex predators may have stronger suppressive effects on evolutionarily naive species than coevolved species. Our results highlight how disease in one species can affect the broader ecosystem. We show that apex predators not only regulate native species but can also confer resistance to the impacts of invasive populations. Apex predators could therefore be a powerful but underutilised tool to prevent biodiversity loss.

EVIDENCE OF LEPTOSPIRA SEROVARS IN WILDLIFE AND LEPTOSPIRAL DNA IN WATER SOURCES IN A NATURAL AREA IN EAST-CENTRAL ILLINOIS, USA.

We identified seven Leptospira serovars in wildlife and the presence of leptospiral DNA in water sources at a natural area within a fragmented habitat in Illinois, US. These serovars have been implicated in domestic animal and human leptospirosis, a reemerging zoonotic disease, whose reservoirs include wildlife and domestic animals. We live trapped medium-sized mammals (n=351) near building (H-sites) or forest sites (F-sites). Using serology, we evaluated exposure to Leptospira (L. interrogans serovars Autumnalis, Bratislava, Canicola, Icterohaemorrhagiae, Pomona; L. kirschneri serovar Grippotyphosa; L. borgpetersenii serovar Hardjo). Using PCR, we tested for the presence of leptospires in eight water samples (ponds, creeks, and rainwater runoff) collected near trapping sites. We identified antibody titers in raccoons (Procyon lotor; 121/221) and Virginia opossums (Didelphis virginiana; 60/112), but not in feral cats (Felis catus; 0/18). We found significant differences in overall Leptospira seroprevalence between years (P=0.043) and animal's age in 2008 (P=0.005) and 2009 (P=0.003). Serovars Autumnalis, Bratislava, and Grippotyphosa showed significant differences among age groups with the highest seroprevalence in adults. Females had a higher seroprevalence for Icterohaemorragiae in 2008 (P=0.003) and Hardjo in 2009 (P=0.041). Risk of exposure to Leptospira was higher at F-sites compared to H-sites (odds ratio 2.3, 95% confidence interval 1.3-3.9, P=0.002). We captured more animals with titers >1:800 at H-sites, but there was no association between titer levels and capture site. Six of eight water sources were Leptospira-positive; however, there was no correlation between trapping locations of seropositive animals and positive water sources. Natural areas create opportunities for interspecies interactions, favoring leptospires transmission across species. Understanding that Leptospira serovars are present in natural areas is an integral part of the safe human and pet recreational use of these areas. Our study should raise awareness and build on public education designed to prevent disease transmission between species.