Epidemiological Relevance of Colletotrichum Species Isolated from Glomerella Leaf Spot Causing Symptoms in Apple Fruit.

Colletotrichum isolates from apple leaves with symptoms of Glomerella leaf spot (GLS) can cause fruit rot and several small lesion spots, here called Colletotrichum fruit spot (CFS). This work investigated the epidemiological relevance of Colletotrichum species obtained from leaves with GLS in causing diseases in immature apple fruit by comparing different fruit sizes (phenological stages) for symptom development. In the first experiment, five Colletotrichum species were inoculated in 'Gala' (Ø = 5.5 cm) and 'Eva' (Ø = 4.8 cm) fruit in the field (2016/17 season). Subsequently, C. chrysophilum and C. nymphaeae were inoculated in fruit of different sizes (Ø = 2.4 to 6.3 cm) in the field (2017/18 and 2021/22 seasons) and in the laboratory according to the phenological stages of growing fruit. At harvest of the immature inoculated fruit in the field, only CFS symptoms were observed in both cultivars. For Gala, the CFS incidence reached 50% regardless of season, pathogen species, and fruit size. For Eva, CFS symptoms were observed after inoculation with C. melonis in the 2016/17 season and in smaller fruit inoculated with C. chrysophilum and C. nymphaeae in 2021/22. During postharvest, bitter rot symptoms developed, but did not seem to come from CFS symptoms. It can be concluded that the Gala cultivar has a high susceptibility to CFS caused by the two Colletotrichum species of the greatest epidemiological importance for GLS in Brazil in all fruit sizes tested.

Protective efficacy of the peptide Subolesin antigen against the cattle tick Rhipicephalus microplus under natural infestation.

The cattle tick Rhipicephalus microplus affect animal health, welfare, and cattle production in tropical and subtropical zones of the world. Anti-tick vaccines have been an effective alternative for cattle tick control instead of traditional chemical products. To date, Subolesin antigen has shown efficacy for the control of tick infestation in cattle, and previous studies showed that one peptide derived from this protein has demonstrated to elicit a strong and specific humoral immune response. Based on these findings, herein we characterized the efficacy of the peptide Subolesin for the control of cattle tick, R. microplus infestation under field conditions. Twenty-four female calves were assigned to four experimental groups and immunized with three subcutaneous doses of the peptide Subolesin, Bm86, both antigens (dual vaccine) and adjuvant/saline alone, respectively. Serum antibody levels (IgG) were assessed by ELISA and confirmed by Western blot; also, reproductive performance of naturally infested R. microplus was determined. The results showed that immunizations with the experimental antigens reduced tick infestations with vaccine's efficacy of 67 % (peptide Subolesin), 56 % (Bm86), and 49 % (dual vaccine) based on adult tick numbers, oviposition, and egg fertility between vaccinated and control animals. Peptide Subolesin-immunized calves developed a strong humoral immune response expressed by high anti-pSubolesin IgG levels, and the Western blot analysis confirmed that it is immunogenic. Cattle receiving Bm86 and dual vaccine showed less protection, although Bm86 was within the range reported previously. The negative correlation between antibody levels and reduction of naturally infested R. microplus strongly suggested that the effect of the vaccine was the result of the antibody response in immunized cattle. In conclusion, it was demonstrated that the peptide Subolesin induced a specific immune response in cattle under field conditions, resulting in reduced R. microplus populations in subsequent generations. Finally, integrated tick control must consider anti-tick vaccines as a cost-effective, sustainable, and successful tool for controlling cattle tick infestations.

Qualidade da silagem de sorgo no período de desensilagem: avaliação de híbridos, estratos do silo e estrutura da massa / Quality of sorghum silage in the feed-out period: evaluation of hybrids, silo layers, and mass structure

O objetivo deste trabalho foi avaliar a qualidade químico-fermentativa da silagem de dois híbridos de sorgo nos diferentes estratos da face do silo e a interferência do tempo de exposição aeróbia da face do silo estruturada e/ou desestruturada (após desensilagem). A silagem do híbrido AG-2005E apresentou maior teor de proteína bruta e pH mais elevado em comparação à do híbrido AG-60298 (6,33% e 4,0 contra 6,06% e 3,8, respectivamente). As silagens do estrato de 0 a 20 cm tinham o menor teor de matéria seca e o maior teor de proteína bruta (38% e 6,35%, respectivamente). O pH não diferiu entre os estratos e o teor de NH3 foi maior no estrato de 60 a 80 cm (4,5%). Com o avanço das 72 horas de exposição aeróbia, a silagem desestruturada apresentou maior incremento de temperatura em relação à silagem da face estruturada, mas esse tempo não foi suficiente para mostrar diferenças entre os teores de matéria seca, matéria mineral, NH3 e pH. O híbrido de sorgo e o estrato do silo interferem diretamente na qualidade químico-fermentativa da silagem. Após a exposição aeróbia, a silagem do painel do silo estruturado parece sofrer menor interferência em relação à silagem após ser desensilada.

This study aimed to evaluate the chemical and fermentation quality of silage from two sorghum hybrids in different layers of the silo feed-out face, as well as the interference of the aerobic exposure time of structured and/or unstructured silo face (after feed-out). The AG-2005E hybrid silage presented higher crude protein content and higher pH compared to hybrid AG-60298 (6.33% and 4.0 versus 6.06% and 3.8, respectively). Silages from stratum 0 to 20cm had the lowest dry matter content and the highest crude protein content (38% and 6.35%, respectively). The pH did not differ between layers, and the NH3 content was higher in the stratum 60 to 80cm (4.5%). During 72 hours of aerobic exposure, the unstructured silage presented a higher increase in temperature than the silage from the structured face, but this period was not enough to result in differences in dry matter, mineral matter, NH3, and pH. The chemical and fermentation quality of the silage was directly influenced by hybrid and silo stratum. After aerobic exposure, silage from the structured face seems to suffer less interference compared to the silo feed-out face.

Quality of sorghum silage in the feed-out period: evaluation of hybrids, silo layers, and mass structure / Qualidade da silagem de sorgo no período de desensilagem: avaliação de híbridos, estratos do silo e estrutura da massa

This study aimed to evaluate the chemical and fermentation quality of silage from two sorghum hybrids in different layers of the silo feed-out face, as well as the interference of the aerobic exposure time of structured and/or unstructured silo face (after feed-out). The AG-2005E hybrid silage presented higher crude protein content and higher pH compared to hybrid AG-60298 (6.33% and 4.0 versus 6.06% and 3.8, respectively). Silages from stratum 0 to 20cm had the lowest dry matter content and the highest crude protein content (38% and 6.35%, respectively). The pH did not differ between layers, and the NH3 content was higher in the stratum 60 to 80cm (4.5%). During 72 hours of aerobic exposure, the unstructured silage presented a higher increase in temperature than the silage from the structured face, but this period was not enough to result in differences in dry matter, mineral matter, NH3, and pH. The chemical and fermentation quality of the silage was directly influenced by hybrid and silo stratum. After aerobic exposure, silage from the structured face seems to suffer less interference compared to the silo feed-out face.

O objetivo deste trabalho foi avaliar a qualidade químico-fermentativa da silagem de dois híbridos de sorgo nos diferentes estratos da face do silo e a interferência do tempo de exposição aeróbia da face do silo estruturada e/ou desestruturada (após desensilagem). A silagem do híbrido AG-2005E apresentou maior teor de proteína bruta e pH mais elevado em comparação à do híbrido AG-60298 (6,33% e 4,0 contra 6,06% e 3,8, respectivamente). As silagens do estrato de 0 a 20 cm tinham o menor teor de matéria seca e o maior teor de proteína bruta (38% e 6,35%, respectivamente). O pH não diferiu entre os estratos e o teor de NH3 foi maior no estrato de 60 a 80 cm (4,5%). Com o avanço das 72 horas de exposição aeróbia, a silagem desestruturada apresentou maior incremento de temperatura em relação à silagem da face estruturada, mas esse tempo não foi suficiente para mostrar diferenças entre os teores de matéria seca, matéria mineral, NH3 e pH. O híbrido de sorgo e o estrato do silo interferem diretamente na qualidade químico-fermentativa da silagem. Após a exposição aeróbia, a silagem do painel do silo estruturado parece sofrer menor interferência em relação à silagem após ser desensilada.

Evaluation of consistent use, barriers to use, and microbiological effectiveness of three prototype household water treatment technologies in Haiti, Kenya, and Nicaragua.

Household water treatment (HWT) can improve drinking water quality and reduce diarrheal disease. New HWT technologies are typically evaluated under ideal conditions; however, health gains depend on consistent, effective household use, which is less often evaluated. We conducted four evaluations of three prototype HWT technologies: two filters and one electrochlorinator. Evaluations consisted of a baseline survey, HWT distribution to households (ranging from 60 to 82), and four visits (ranging from 1 week-14 months after distribution). Each visit included a survey, observation of treated water presence (confirmed use), and microbiological analysis of treated and untreated samples for E. coli. Consistent use was defined as the proportion of total visits with confirmed use. Overall, confirmed use declined 2.54% per month on average, and 2-72% of households demonstrated 100% consistent use. Consistent use was positively associated with baseline HWT knowledge and practice and belief that drinking water was unsafe, and negatively associated with technological problems. Reported barriers to use were behavioral, such as forgetting or when outside the home, and technological failures. Technologies demonstrated 68-96% E. coli reductions, with 18-70% of treated samples having detectable E. coli. Results highlight the importance of household use evaluations within prototype HWT technology design cycles, the need for standard evaluation metrics, and difficulties in achieving both consistent use and microbiological effectiveness with HWT technologies.

Enterobacter sp. strain Fs-11 adapted to diverse ecological conditions and promoted sunflower achene yield, nutrient uptake, and oil contents.

Plant growth-promoting rhizobacteria are under extensive investigation to supplement the chemical fertilizers due to cost-effective and eco-friendly nature. However, their consistency in heterogeneous soil and diverse ecological settings is unclear. The current study presents in vitro and field evaluation of pre-characterized PGPR strain Enterobacter sp. Fs-11 (GenBank accession # GQ179978) in terms of its potential to enhance sunflower yield and oil contents under diverse environmental conditions. Under in vitro conditions, strain Fs-11 showed optimal growth at a range of temperature (15 to 40 °C) and pH values (6.5 to 8.5). Extracellular and intracellular localizations of the strain Fs-11 in sunflower root cortical cells through transmission electron microscopy confirmed its epiphytic and endophytic colonization patterns, respectively. In field experiments, conducted at three different agro-climatic locations, inoculation of strain Fs-11 at 50% reduced NP fertilizer resulted in a significant increase in growth, achene yield, nutrient uptake, and oil contents. Inoculation also responded significantly in terms of increase in mono- and polyunsaturated fatty acids (oleic and linoleic acids, respectively) without rising saturated fatty acid (palmitic and stearic acids) contents. We concluded that Enterobacter sp. Fs-11 is a potential candidate for biofertilizer formulations to supplement chemical fertilizer requirements of sunflower crop under diverse climatic conditions.

A scoping review on the field validation and implementation of rapid diagnostic tests for vector-borne and other infectious diseases of poverty in urban areas.

BACKGROUND: Health personnel face challenges in diagnosing vector-borne and other diseases of poverty in urban settings. There is a need to know what rapid diagnostic technologies are available, have been properly assessed, and are being implemented to improve control of these diseases in the urban context. This paper characterizes evidence on the field validation and implementation in urban areas of rapid diagnostics for vector-borne diseases and other diseases of poverty. MAIN BODY: A scoping review was conducted. Peer-reviewed and grey literature were searched using terms describing the targeted infectious diseases, diagnostics evaluations, rapid tests, and urban setting. The review was limited to studies published between 2000 and 2016 in English, Spanish, French, and Portuguese. Inclusion and exclusion criteria were refined post hoc to identify relevant literature regardless of study design and geography. A total of 179 documents of the 7806 initially screened were included in the analysis. Malaria (n = 100) and tuberculosis (n = 47) accounted for the majority of studies that reported diagnostics performance, impact, and implementation outcomes. Fewer studies, assessing mainly performance, were identified for visceral leishmaniasis (n = 9), filariasis and leptospirosis (each n = 5), enteric fever and schistosomiasis (each n = 3), dengue and leprosy (each n = 2), and Chagas disease, human African trypanosomiasis, and cholera (each n = 1). Reported sensitivity of rapid tests was variable depending on several factors. Overall, specificities were high (> 80%), except for schistosomiasis and cholera. Impact and implementation outcomes, mainly acceptability and cost, followed by adoption, feasibility, and sustainability of rapid tests are being evaluated in the field. Challenges to implementing rapid tests range from cultural to technical and administrative issues. CONCLUSIONS: Rapid diagnostic tests for vector-borne and other diseases of poverty are being used in the urban context with demonstrated impact on case detection. However, most evidence comes from malaria rapid diagnostics, with variable results. While rapid tests for tuberculosis and visceral leishmaniasis require further implementation studies, more evidence on performance of current tests or development of new alternatives is needed for dengue, Chagas disease, filariasis, leptospirosis, enteric fever, human African trypanosomiasis, schistosomiasis and cholera.

Validity of a portable force platform for assessing biomechanical parameters in three different tasks.

The aim of this study was to determine the precision and accuracy of the vertical and anterior-posterior force components of the portable PASCO PS-2142 force plate. Impulse, peak force, and time to peak force were assessed and compared to a gold standard force plate in three different tasks: vertical jump, forward jump, and sprint start. Two healthy male participants performed ten trials for each task, resulting in 60 trials. Data analyses revealed good precision and accuracy for the vertical component of the portable force plate, with relative bias and root mean square (RMS) error values nearly the same in all tasks for the impulse, time to peak force, and peak force parameters. Precision and accuracy of the anterior-posterior component were lower for the impulse and time to peak force, with relative bias and RMS error values nearly the same between tasks. Despite the lower precision and accuracy of the anterior-posterior component of the portable force plate, these errors were systematic, reflecting a good repeatability of the measure. In addition, all variables presented good agreement between the portable and gold standard platforms. Our results provide a good perspective for using the aforementioned portable force plate in sports and clinical biomechanics.

Field Evaluation of Different Wavelengths Light-Emitting Diodes as Attractants for Adult Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae).

In recent years, light traps with light-emitting diodes (LEDs) have been widely used in integrated pest management. The spiralling whitefly, Aleurodicus dispersus Russell, a highly invasive pest which causes heavy damage to fruit trees and ornamental plants, exhibits positive phototaxis, and light trap is the most appropriate tool for monitoring. We evaluated the use of LEDs as an inexpensive light source and examined the relationship between the captured number and the population density of adult A. dispersus in the field. We found that the violet (405 nm) LED traps captured the most adults of A. dispersus, and the captured numbers were significantly higher than those of blue (460 nm), green (520 nm), yellow (570 nm), and red (650 nm) LED traps. The adults of A. dispersus captured by light traps equipped with violet LEDs and smeared with liquid paraffin had a significant positive correlation with the population density of adult A. dispersus in a guava orchard, with a correlation coefficient of 0.828. In general, the light traps with 15 violet LED bulbs hung into 550-mL plastic bottles and smeared with liquid paraffin were the portable devices for attraction of adult A. dispersus. The results have potential use for improving the efficiency of light traps at attracting and trapping the adult spiralling whitefly.

Evaluación de plantas de Curcuma longa L. obtenidas por cultivo de tejidos en condiciones de organopónico / Evaluation of Curcuma longa L. plants obtained by tissue culture in organoponic conditions

La cúrcuma es una planta utilizada como condimento y colorante que posee múltiples propiedades medicinales. La evaluación de la estabilidad genética y la respuesta en condiciones de producción constituye una etapa muy importante en los sistemas de propagación de plantas por cultivo de tejidos. En este trabajo se evaluó la respuesta morfoagronómica de plantas de cúrcuma obtenidas por cultivo de tejido y por rizomas en condiciones de organopónico. Los resultados muestran una alta supervivencia (100%), para las plantas provenientes del cultivo in vitro, así como para las obtenidas a partir de los rizomas. Se logró un mayor crecimiento de las plantas propagadas por el cultivo de tejidos durante todo el ciclo de cultivo, sin cambios en las características morfológicas evaluadas. Los rendimientos en las plantas provenientes del cultivo de tejidos fueron superiores en comparación con las plantas propagadas por el método tradicional.

Turmeric is a plant uses like condiment and coloring. It has multiples medicinal properties. The genetic stability and response in production conditions is an important stage in plant propagation system by tissue culture. In this paper was evaluated the morphoagronomic response of turmeric plants obtained by tissue culture in organoponic conditions. Results show a high survival (100%) for a plant from in vitro culture as well as for the plant obtained by rizomes . A bigger growth of the plants obtained by tissue culture was observed during the whole cultured cycle without changes in the morphologycal characteristic evaluated. The yields of the plants obtained by tissue culture were higher that the plants planted by the traditional method.